Although the pharmacological benefits of fig ( Ficus carica ) leaf, belonging to the Moraceae family, such as antioxidant, anti-inflammatory, antibacterial, antifungal, antidiabetic, and anticancer properties are well-documented, studies on their potential effects on wound healing and the molecular mechanisms involved in this process are limited. This study aims to fill this gap by exploring the anti-inflammatory effects of black fig leaf on wound healing and its potential to promote dermal regeneration through histological and genetic analyses. Excisional skin wounds were created in Wistar albino rats, divided into three groups: control (C), cold cream (CC), and 5% black fig leaf cream (FCC). The gene expression levels of cytokines involved in the inflammatory process (interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α) were evaluated using real-time PCR, and protein expression levels were assessed through immunohistochemistry. Histological evaluation of the wound site was performed using hematoxylin and eosin (H&E) and Masson trichrome staining. Molecular analysis showed that the 5% black fig leaf cream exhibited significantly higher anti-inflammatory activity than the control groups. Histopathological examination revealed increased collagen production, angiogenesis, and re-epithelialization, along with reduced inflammatory cell density and bleeding compared to controls. Due to its observed anti-inflammatory activity, the 5% black fig leaf cream may support skin wound healing and has the potential to be a therapeutic agent for skin wounds.

This study aims to evaluate the cytotoxic effects of methanol (AOME) and ethyl acetate (AOEE) extracts obtained from the flowers of Althaea officinalis L., which is known for its high antioxidant capacity, on prostate cancer cell lines (LNCaP and PC3), as well as their inhibitory effects on cell migration and invasion, and their influence on pro-apoptotic gene expression. Cell viability was assessed using the MTT assay, while cell migration and invasion were analyzed using the scratch assay. Changes in pro-apoptotic gene expression were evaluated by real-time PCR (RT-qPCR), as determined at the IC₅₀ concentrations obtained from cytotoxicity tests. The results showed that AOME and AOEE induced dose-dependent cytotoxicity in both cell lines. The IC₅₀ values were found to be 1.1×10⁻³ μg/mL (AOME) and 11×10⁻⁵ μg/mL (AOEE) for PC3 cells, and 0.21×10⁻³ μg/mL (AOME) and 0.45×10⁻³ μg/mL (AOEE) for LNCaP cells. Scratch assay analyses demonstrated that both extracts significantly inhibited cell migration and invasion compared to the control group. Additionally, RT-qPCR analyses revealed that pro-apoptotic gene expression was significantly increased, and anti-apoptotic gene expression was significantly decreased in treated cells (p

This study aimed to investigate the effect of black fig (Ficus carica) leaf extract on the skin wound healing process. In the survey, Wistar albino female rats with skin wounds on their backs according to the circular excisional wound model were divided into three groups: control, cold cream, and 5% black fig leaf cream. Following wound formation, tissue samples were collected from each group for analysis on the 3rd, 7th, and 14th days. Inflammatory cell density, bleeding, fibroblast proliferation, epithelialization, and collagen formation in the collected wound tissues were determined by hematoxylin and eosin staining and Masson's trichrome staining; VEGF and COL I gene and protein expression levels involved in the wound healing process were determined by real-time PCR and immunohistochemical analysis. As a result of the analysis, it was determined that high re-epithelialization, fibroblast proliferation, angiogenesis development, and collagen formation were observed in the FCC group compared to the C and CC groups. At the end of the study, the positive effect of black fig leaf on wound healing was also shown genetically and histopathologically. Further studies are needed to explore the potential of black fig leaves as a therapeutic drug for the treatment of skin wounds.

Objective: This study aimed to evaluate the cytotoxic and apoptosis-inducing effects of lisinopril and amlodipine in a human hepatocellular carcinoma (Hep3B) cell model. Materials and Methods: The cytotoxic effects of Hep3B cells treated with lisinopril and amlodipine were assessed using the MTT assay. Cell viability was analyzed at 24, 48, and 72 hours, and IC₅₀ values were calculated. Apoptosis-related gene expression levels, including Bcl-2, Bcl-xL, Bax, and Bak, were determined using RT-qPCR. Statistical analyses were performed using one-way ANOVA, and differences with p