High-throughput phenotyping has become the frontier to accelerate breeding through linking genetics to crop growth estimation, which requires accurate estimation of leaf area index (LAI). This study developed a hybrid method to train the random forest regression (RFR) models with synthetic datasets generated by a radiative transfer model to estimate LAI from UAV-based multispectral images. The RFR models were evaluated on both (i) subsets from the synthetic datasets and (ii) observed data from two field experiments (i.e., Exp16, Exp19). Given the parameter ranges and soil reflectance are well calibrated in synthetic training data, RFR models can accurately predict LAI from canopy reflectance captured in field conditions, with systematic overestimation for LAI<2 due to background effect, which can be addressed by applying background correction on original reflectance map based on vegetation-background classification. Overall, RFR models achieved accurate LAI prediction from background-corrected reflectance for Exp16 (correlation coefficient () of 0.95, determination coefficient () of 0.90~0.91, root mean squared error (RMSE) of 0.36~0.40 m² m^-2, relative root mean squared error (RRMSE) of 25~28%) and less accurate for Exp19 ( =0.80~0.83, = 0.63~0.69, RMSE of 0.84~0.86 m² m^-2, RRMSE of 30~31%). Additionally, RFR models correctly captured spatiotemporal variation of observed LAI as well as identified variations for different growing stages and treatments in terms of genotypes and management practices (i.e., planting density, irrigation, and fertilization) for two experiments. The developed hybrid method allows rapid, accurate, nondestructive phenotyping of the dynamics of LAI during vegetative growth to facilitate assessments of growth rate including in breeding program assessments.

A major challenge for the estimation of crop traits (biophysical variables) from canopy reflectance is the creation of a high-quality training dataset. To address this problem, this research investigated a conceptual framework by integrating a crop growth model with a radiative transfer model to introduce biological constraints in a synthetic training dataset. In addition to the comparison of two datasets without and with biological constraints, we also investigated the effects of observation geometry, retrieval method, and wavelength range on estimation accuracy of four crop traits (leaf area index, leaf chlorophyll content, leaf dry matter, and leaf water content) of wheat. The theoretical analysis demonstrated potential advantages of adding biological constraints in synthetic training datasets as well as the capability of deep learning. Additionally, the predictive models were validated on real unmanned aerial vehicle-based multispectral images collected from wheat plots contrasting in canopy structure. The predictive model trained over a synthetic dataset with biological constraints enabled the prediction of leaf water content from using wavelengths in the visible to near infrared range based on the correlations between crop traits. Our findings presented the potential of the proposed conceptual framework in simultaneously retrieving multiple crop traits from canopy reflectance for applications in precision agriculture and plant breeding.