Sweet basil (Ocimum basilicum L.) is one of the most well-known aromatic herbs, which are economically important for food and pharmaceutical purposes. In vitro regeneration protocols are a fundamental part of molecular approaches, such as genome editing, which are used to enhance crop quality and pathogen resistance. In this research, in vitro regeneration methods were developed to examine the morphogenic aptitude of four different explant types from five commercial cvs of Ocimum basilicum L. (‘Prospera’, ‘Paoletto’, ‘Italiko FT’, ‘Dark opal’, and ‘Bolloso napoletano’). ‘Prospera’ showed the highest direct regeneration efficiency in all of the explant types (100% in the roots, 36% ± 0.02 in the cotyledons, 7.5% ± 0.2 in the hypocotyls, and 50% ± 0.04 in the cotyledonary nodes). The roots were found to be the most effective explant type, producing nodule-like meristems (100% in ‘Prospera’ and ‘FT Italiko’, 95.24% ± 0.01 in ‘Bolloso napoletano’), as precursors of shoots. Histological analysis was confirmed to be a suitable method to detect meristematic activity during the early morphogenic process and to evaluate the explants’ regeneration potential.

Ornamental plants constitute an important sector of the agricultural market, with global relevance. The impacts of climate change also affect flowering plants, increasing susceptibility to stress conditions. The most common effects are related to germination capacity and uniformity, which can indirectly compromise aesthetic value. Seed priming represents a promising environmentally friendly strategy to improve germination performance and stress tolerance in ornamental plants. This review gives an overview of the most recent advances in priming applied to ornamental species, highlighting the physiological, biochemical, and molecular impacts of the technique. Priming approaches can differ (e.g., hydro-, osmotic, hormonal, nano-based), with the common aim of enhancing germination efficiency, seed vigor, and promoting greater uniformity and quality of plantlets under stress conditions. Up to now, a bottleneck in the use of this promising tool has been represented by different responses related to species and protocol standardization. Molecular evaluation is required to monitor the long-term effectiveness of priming. The use of priming represents a sustainable and low-cost tool to enhance the adaptability of ornamental species in stress conditions, supporting the transition to new sustainable solutions for the future of ornamental plant production.

This study evaluated the effect of the culture system (temporary immersion bioreactor (TIB) vs. solid culture) on the micropropagation efficiency of two Lachenalia cultivars, ‘Rainbow Bells’ and ‘Riana’. Morphological and biochemical responses were analyzed under different immersion frequencies (15 min every 24 h, 15 min every 8 h, and 5 min every 8 h) and on solid medium. TIB, regardless of the immersion frequency, was more efficient in terms of biomass growth (3.27–3.95-fold increase) and the number of obtained bulblets (17.80–19.08 bulbs). The response to culture conditions was genotype-dependent. ‘Rainbow Bells’ exhibited higher biomass growth and bulblet number, whereas ‘Riana’ produced fewer but heavier bulblets (4.39 and 2.09 of biomass growth, 23.19 and 9.97 bulbs per 1 g, 0.23 and 0.31 g per bulb, respectively, for ‘Rainbow Bells’ and Riana’). The most effective bulblet multiplication was obtained under the 1 × 15 min regime for ‘Rainbow Bells’; the same frequency promoted bulblet enlargement in ‘Riana’. Principal component analysis (PCA) explained 77% of total variance, revealing strong genotype separation: ‘Rainbow Bells’ clustered with traits linked to growth intensity and phenolic accumulation, while ‘Riana’ correlated with bulb storage parameters. The results support the use of TIBs to improve Lachenalia micropropagation, bulb quality, and future automation, and indicate that further research should focus on optimizing culture parameters for each genotype.