Nowadays, the rapid improvements in the medical and pharmaceutical fields increase the diversity and use of drugs. However, problems such as the use of multiple or combined drugs in the treatment of diseases and insensible use of over-the-counter drugs have caused concerns about the side-effect profiles and therapeutic ranges of drugs and environmental contamination and pollution problems due to pharmaceuticals waste. Therefore, the analysis of drugs in various media such as biological, pharmaceutical, and environmental samples is an important topic of discussion. Electrochemical methods are advantageous for sensor applications due to their easy application, low cost, versatility, high sensitivity, and environmentally-friendliness. Carbon nanomaterials such as diamond-like carbon thin films, carbon nanotubes, carbon nanofibers, graphene oxide, and nanodiamonds are used to enhance the performance of the electrochemical sensors with catalytic effects. To further improve this effect, it is aimed to create hybrid platforms by using different carbon nanomaterials together or with materials such as conductive polymers and ionic liquids. In this review, the most used carbon nanoforms will be evaluated in terms of electrochemical characterizations and physicochemical properties. Furthermore, the effect of hybrid platforms developed in the most recent studies on electrochemical sensors will be examined and evaluated in terms of drug analysis studies in the last five years.

Drug and biomolecule analysis with high precision, fast response, not expensive, and user-friendly methods have been very important for developing technology and clinical applications. Electrochemical methods are highly capable for assaying the concentration of electroactive drug or biomolecule and supply excellent knowledge concerning its physical and chemical properties such as electron transfer rates, diffusion coefficients, electron transfer number, and oxidation potential. Electrochemical methods have been widely applied because of their accuracy, sensitivity, cheapness, and can applied on-site determinations of various substances. The progress on electronics has allowed developing reliable, more sensitive and less expensive instrumentations, which have significant contribution in the area of drug development, drug and biomolecule analysis. The developing new sensors for electrochemical analysis of these compounds have growing interest in recent years. Screen-printed based electrodes have a great interest in electrochemical analysis of various drugs and biomolecules due to their easy manufacturing procedure of the electrode allow the transfer of electrochemical laboratory experiments for disposable on-site analysis of some compounds. Paper based electrodes are also fabricated by new technology. They can be preferred due to their easy, cheap, portable, disposable, and offering high sensitivity properties for many application field such as environmental monitoring, food quality control, clinical diagnosis, drug, and biomolecules analysis. In this review, the recent electrochemical drug and biomolecule (DNA, RNA, µRNA, Biomarkers, etc.) studies will be presented that involve new trend disposable electrodes.

Bacterial and viruses pathogens are a significant hazard to human safety and health. In the imaging and detection of pathogenic microorganisms, the application of fluorescent nanoparticles is very useful. Carbon dots and quantum dots are preferred in this regard as labels, amplifiers, and/or electrode modifiers because of their outstanding features. However, precise diagnostics to identify numerous harmful bacteria simultaneously still face considerable hurdles, yet it is an inevitable issue. With the growing development of biosensors, nanoproduct-based bio-sensing has recently become one of the most promising methods for accurately identifying and quantifying various pathogens at low cost, high sensitivity, and selectivity, with time savings. The most recent applications of carbon dots in optical and electrochemical-based sensors are discussed in this review, along with some examples of pathogen sensors. Graphical Abstract