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In order to improve their bioapplications, inorganic nanoparticles (NPs) are usually functionalized with specific biomolecules. Peptides with short amino acid sequences have attracted great attention in the NP functionalization since they are easy to be synthesized on a large scale by the automatic synthesizer and can integrate various functionalities including specific biorecognition and therapeutic function into one sequence. Conjugation of peptides with NPs can generate novel theranostic/drug delivery nanosystems with active tumor targeting ability and efficient nanosensing platforms for sensitive detection of various analytes, such as heavy metallic ions and biomarkers. Massive studies demonstrate that applications of the peptide–NP bioconjugates can help to achieve the precise diagnosis and therapy of diseases. In particular, the peptide–NP bioconjugates show tremendous potential for development of effective anti-tumor nanomedicines. This review provides an overview of the effects of properties of peptide functionalized NPs on precise diagnostics and therapy of cancers through summarizing the recent publications on the applications of peptide–NP bioconjugates for biomarkers (antigens and enzymes) and carcinogens (e.g., heavy metallic ions) detection, drug delivery, and imaging-guided therapy. The current challenges and future prospects of the subject are also discussed.
Xiaotong Li; Minghong Jian; Yanhong Sun; Qunyan Zhu; Zhenxin Wang. The Peptide Functionalized Inorganic Nanoparticles for Cancer-Related Bioanalytical and Biomedical Applications. Molecules 2021, 26, 3228 .
AMA StyleXiaotong Li, Minghong Jian, Yanhong Sun, Qunyan Zhu, Zhenxin Wang. The Peptide Functionalized Inorganic Nanoparticles for Cancer-Related Bioanalytical and Biomedical Applications. Molecules. 2021; 26 (11):3228.
Chicago/Turabian StyleXiaotong Li; Minghong Jian; Yanhong Sun; Qunyan Zhu; Zhenxin Wang. 2021. "The Peptide Functionalized Inorganic Nanoparticles for Cancer-Related Bioanalytical and Biomedical Applications." Molecules 26, no. 11: 3228.
In this work, a simple enzyme-free flow cytometric assay (termed as TSDR-based flow cytometric assay) has been developed for the detection of papillary thyroid carcinoma (PTC)-related microRNA (miRNA), hsa-miR-146b-5p with high performance through the toehold-mediated strand displacement reaction (TSDR) on magnetic beads (MBs). The complementary single-stranded DNA (ssDNA) probe of hsa-miR-146b-5p was first immobilized on the surface of MB, which can partly hybridize with the carboxy-fluorescein (FAM)-modified ssDNA, resulting in strong fluorescence emission. In the presence of hsa-miR-146b-5p, the TSDR is trigged, and the FAM-modified ssDNA is released form the MB surface due to the formation of DNA/RNA heteroduplexes on the MB surface. The fluorescence emission change of MBs can be easily read by flow cytometry and is strongly dependent on the concentration of hsa-miR-146b-5p. Under optimal conditions, the TSDR-based flow cytometric assay exhibits good specificity, a wide linear range from 5 to 5000 pM and a relatively low detection limit (LOD, 3σ) of 4.21 pM. Moreover, the practicability of the assay was demonstrated by the analysis of hsa-miR-146b-5p amounts in different PTC cells and clinical PTC tissues.
Yue Wu; Jiaxue Gao; Jia Wei; Jingjing Zhou; Xianying Meng; Zhenxin Wang. Development of Flow Cytometric Assay for Detecting Papillary Thyroid Carcinoma Related hsa-miR-146b-5p through Toehold-Mediated Strand Displacement Reaction on Magnetic Beads. Molecules 2021, 26, 1628 .
AMA StyleYue Wu, Jiaxue Gao, Jia Wei, Jingjing Zhou, Xianying Meng, Zhenxin Wang. Development of Flow Cytometric Assay for Detecting Papillary Thyroid Carcinoma Related hsa-miR-146b-5p through Toehold-Mediated Strand Displacement Reaction on Magnetic Beads. Molecules. 2021; 26 (6):1628.
Chicago/Turabian StyleYue Wu; Jiaxue Gao; Jia Wei; Jingjing Zhou; Xianying Meng; Zhenxin Wang. 2021. "Development of Flow Cytometric Assay for Detecting Papillary Thyroid Carcinoma Related hsa-miR-146b-5p through Toehold-Mediated Strand Displacement Reaction on Magnetic Beads." Molecules 26, no. 6: 1628.
Osteosarcoma (OS) is a primary malignant bone tumor with high rate of recurrence and lung metastasis. Matrix metalloproteinases (MMPs) are demonstrated as important biomarkers of tumor invasion and metastasis. Herein, a peptide microarray-based fluorescence assay is proposed to profile multiple MMPs (MMP-1, -2, -3, -7, -9 and -13) activities in the progression of OS by using the mouse-bearing xenograft U-2OS and Saos-2 human OSs. The peptide microarray containing different biotinylated peptide substrate spots is fabricated on the poly(glycidyl methacrylate-co-2-hydroxyethyl methacrylate) brush coated zinc oxide nanorod ([email protected](GMA-HEMA) decorated glass slides, and labeled by cyanine 3 (Cy3) modified avidin (Avidin-Cy3) to produce fluorescence signal. Special cleavage of peptide substrate by MMP resulted the decrease of fluorescence signal. The MMPs activities are positively correlated with the change of fluorescence intensity. This method has excellent selectivity and sensitivity, which enables to detect the activities of cellular secreted MMP-1, -2, -3, -7, -9, and -13 with limit of detection downs to 10 pmol L−1, 30 pmol L−1, 113 pmol L−1, 13 pmol L−1, 93 pmol L−1 and 12 pmol L−1, respectively. Furthermore, it is demonstrated that the activity pattern of MMPs in serum closely relevant to the disease progression and type of tumor.
Minghong Jian; Hua Zhang; Xiaotong Li; Zhenxin Wang. Profiling of multiple matrix metalloproteinases activities in the progression of osteosarcoma by peptide microarray-based fluorescence assay on polymer brush coated zinc oxide nanorod substrate. Sensors and Actuators B: Chemical 2021, 330, 129361 .
AMA StyleMinghong Jian, Hua Zhang, Xiaotong Li, Zhenxin Wang. Profiling of multiple matrix metalloproteinases activities in the progression of osteosarcoma by peptide microarray-based fluorescence assay on polymer brush coated zinc oxide nanorod substrate. Sensors and Actuators B: Chemical. 2021; 330 ():129361.
Chicago/Turabian StyleMinghong Jian; Hua Zhang; Xiaotong Li; Zhenxin Wang. 2021. "Profiling of multiple matrix metalloproteinases activities in the progression of osteosarcoma by peptide microarray-based fluorescence assay on polymer brush coated zinc oxide nanorod substrate." Sensors and Actuators B: Chemical 330, no. : 129361.
The advancements of magnetic resonance imaging contrast agents (MRCAs) are continuously driven by the critical needs for early detection and diagnosis of diseases, especially for cancer, because MRCAs improve diagnostic accuracy significantly. Although hydrophilic gadolinium (III) (Gd3+) complex-based MRCAs have achieved great success in clinical practice, the Gd3+-complexes have several inherent drawbacks including Gd3+ leakage and short blood circulation time, resulting in the potential long-term toxicity and narrow imaging time window, respectively. Nanotechnology offers the possibility for the development of nontoxic MRCAs with an enhanced sensitivity and advanced functionalities, such as magnetic resonance imaging (MRI)-guided synergistic therapy. Herein, we provide an overview of recent successes in the development of renal clearable MRCAs, especially nanodots (NDs, also known as ultrasmall nanoparticles (NPs)) by unique advantages such as high relaxivity, long blood circulation time, good biosafety, and multiple functionalities. It is hoped that this review can provide relatively comprehensive information on the construction of novel MRCAs with promising clinical translation.
Xiaodong Li; Yanhong Sun; Lina Ma; Guifeng Liu; Zhenxin Wang. The Renal Clearable Magnetic Resonance Imaging Contrast Agents: State of the Art and Recent Advances. Molecules 2020, 25, 5072 .
AMA StyleXiaodong Li, Yanhong Sun, Lina Ma, Guifeng Liu, Zhenxin Wang. The Renal Clearable Magnetic Resonance Imaging Contrast Agents: State of the Art and Recent Advances. Molecules. 2020; 25 (21):5072.
Chicago/Turabian StyleXiaodong Li; Yanhong Sun; Lina Ma; Guifeng Liu; Zhenxin Wang. 2020. "The Renal Clearable Magnetic Resonance Imaging Contrast Agents: State of the Art and Recent Advances." Molecules 25, no. 21: 5072.
Here, a heteronanostructure ([email protected]) has been constructed for multimodality molecular imaging (T1-/T2-weighted MRI and CT imaging)-guided PTT of cancer by combination of Au-Fe3O4, PDA shell and DTPA-Gd into one nanoplatform.
Fengqin Xu; Xiaodong Li; Hongda Chen; Minghong Jian; Yanhong Sun; Guifeng Liu; Lina Ma; Zhenxin Wang. Synthesis of heteronanostructures for multimodality molecular imaging-guided photothermal therapy. Journal of Materials Chemistry B 2020, 8, 10136 -10145.
AMA StyleFengqin Xu, Xiaodong Li, Hongda Chen, Minghong Jian, Yanhong Sun, Guifeng Liu, Lina Ma, Zhenxin Wang. Synthesis of heteronanostructures for multimodality molecular imaging-guided photothermal therapy. Journal of Materials Chemistry B. 2020; 8 (44):10136-10145.
Chicago/Turabian StyleFengqin Xu; Xiaodong Li; Hongda Chen; Minghong Jian; Yanhong Sun; Guifeng Liu; Lina Ma; Zhenxin Wang. 2020. "Synthesis of heteronanostructures for multimodality molecular imaging-guided photothermal therapy." Journal of Materials Chemistry B 8, no. 44: 10136-10145.
A facile scalable approach is presented for the rational design of multidimensional, multilayered sand-clock-like UCNPs (denoted as UCCKs) bounded with high index facets, with a tunable Nd3+ content, and without a template or multiple complicated reaction steps. This was achieved using the seed-mediated growth and subsequent longitudinal direction epitaxial growth with the assistance of oleic acid and NH4F. The as-formed UCCKs composed of an inner layer (NaYF4:Yb,Er,Ca), an intermediate layer (NaYF4:Yb,Ca), and an outer layer (NaNdF4:Yb,Ca). The outer shell, enriched with Nd3+ sensitizer, augmented the near-infrared (NIR) photon absorption, whereas the intermediate shell, enriched with Yb3+, acted as a bridge for energy transfer from Nd3+ to Er3+ emitter in the inner core alongside with precluding any deleterious energy back-transfer from Er3+ or quenching effect from Nd3+. These unique structural and compositional properties of UCCKs endowed the UCL intensity of UCCKs by 22 and 10 times higher than that of hexagonal UCNP core (NaYF4:Yb,Er,Ca) and hexagonal UCNP core-shell (NaYF4:Yb,Er,[email protected]:Yb,Ca), respectively. Intriguingly, the UCL intensity increased significantly with increasing the content of Nd3+ in the outer shell. The silica-coated UCCKs were used as excellent long-term luminescence probes for the in vitro bioimaging without any noteworthy cytotoxicity. The presented approach may pave the road for controlling the synthesis of multidimensional UCCKs for various applications. We developed novel multidimensional multilayered sand-clock-like upconversion nanostructures composed of a spherical inner core (NaYF4:Yb,Er,Ca), hexagonal intermediate shell (NaYF4:Yb,Ca) and two up-down outer shell (NaNdF4:Yb,Ca) with controllable Nd3+ as an efficient and safe probe for bioimaging applications without any quenching effect.
Murad M. A. Abualrejal; Kamel Eid; Aboubakr M. Abdullah; Abdulqawi Ahmed Numan; Hongda Chen; Hua Zhang; Zhenxin Wang. Smart design of exquisite multidimensional multilayered sand-clock-like upconversion nanostructures with ultrabright luminescence as efficient luminescence probes for bioimaging application. Microchimica Acta 2020, 187, 1 -13.
AMA StyleMurad M. A. Abualrejal, Kamel Eid, Aboubakr M. Abdullah, Abdulqawi Ahmed Numan, Hongda Chen, Hua Zhang, Zhenxin Wang. Smart design of exquisite multidimensional multilayered sand-clock-like upconversion nanostructures with ultrabright luminescence as efficient luminescence probes for bioimaging application. Microchimica Acta. 2020; 187 (9):1-13.
Chicago/Turabian StyleMurad M. A. Abualrejal; Kamel Eid; Aboubakr M. Abdullah; Abdulqawi Ahmed Numan; Hongda Chen; Hua Zhang; Zhenxin Wang. 2020. "Smart design of exquisite multidimensional multilayered sand-clock-like upconversion nanostructures with ultrabright luminescence as efficient luminescence probes for bioimaging application." Microchimica Acta 187, no. 9: 1-13.
This review highlights recent advances in nanocomposite-based electrochemical aptasensors for the detection of toxins with high assaying performance.
Hongyan Shan; Xiaotong Li; Lin Liu; DaQian Song; Zhenxin Wang. Recent advances in nanocomposite-based electrochemical aptasensors for the detection of toxins. Journal of Materials Chemistry B 2020, 8, 5808 -5825.
AMA StyleHongyan Shan, Xiaotong Li, Lin Liu, DaQian Song, Zhenxin Wang. Recent advances in nanocomposite-based electrochemical aptasensors for the detection of toxins. Journal of Materials Chemistry B. 2020; 8 (27):5808-5825.
Chicago/Turabian StyleHongyan Shan; Xiaotong Li; Lin Liu; DaQian Song; Zhenxin Wang. 2020. "Recent advances in nanocomposite-based electrochemical aptasensors for the detection of toxins." Journal of Materials Chemistry B 8, no. 27: 5808-5825.
A facile method has been developed for the rapid and efficient enrichment of DNAs from different media including synthetic single-strand DNAs (ssDNAs) from buffer solutions and cell-free DNAs (cfDNAs) from blood plasma through electric field-driven adsorption and desorption of DNAs by a polyacrylamide/phytic acid/polydopamine (PAAM/PA/PDA) hydrogel. The as-prepared PAAM/PA/PDA hydrogel possesses regular porosity with a large surface area, strong electric field responsiveness/good conductivity, and a rich aromatic structure, which can be used as an ideal adsorbent for DNA enrichment under a positive electric field. The enriched DNAs can be released efficiently when the positive electric field is converted to a negative electric field. The PAAM/PA/PDA hydrogel-based electrochemical method enables the completion of the process of DNA adsorption and release within 5 min and exhibits reasonable enrichment efficiencies and recovery rates of various DNAs. For instance, the high enrichment sensitivity (0.1 pmol L-1) together with the excellent recovery (>75%) of an ssDNA with 78 nucleotides is obtained. Combined with the PCR amplification technique, the practicability of the as-proposed method is demonstrated by the screening of circulating tumor DNAs (ctDNAs) with a BRAFV600E mutation in cfDNAs from the blood plasma samples of patients with papillary thyroid cancer or thyroid nodule and random patients from a clinical laboratory.
Jia Wei; Zhen Zhao; Jiaxue Gao; Yaoqi Wang; Lina Ma; Xianying Meng; Zhenxin Wang. Polyacrylamide/Phytic Acid/Polydopamine Hydrogel as an Efficient Substrate for Electrochemical Enrichment of Circulating Cell-Free DNA from Blood Plasma. ACS Omega 2020, 5, 5365 -5371.
AMA StyleJia Wei, Zhen Zhao, Jiaxue Gao, Yaoqi Wang, Lina Ma, Xianying Meng, Zhenxin Wang. Polyacrylamide/Phytic Acid/Polydopamine Hydrogel as an Efficient Substrate for Electrochemical Enrichment of Circulating Cell-Free DNA from Blood Plasma. ACS Omega. 2020; 5 (10):5365-5371.
Chicago/Turabian StyleJia Wei; Zhen Zhao; Jiaxue Gao; Yaoqi Wang; Lina Ma; Xianying Meng; Zhenxin Wang. 2020. "Polyacrylamide/Phytic Acid/Polydopamine Hydrogel as an Efficient Substrate for Electrochemical Enrichment of Circulating Cell-Free DNA from Blood Plasma." ACS Omega 5, no. 10: 5365-5371.
Toxin detection is an important issue in numerous fields, such as agriculture/food safety, environmental monitoring, and homeland security. During the past two decades, nanotechnology has been extensively used to develop various biosensors for achieving fast, sensitive, selective and on-site analysis of toxins. In particular, the two dimensional layered (2D) nanomaterials (such as graphene and transition metal dichalcogenides (TMDs)) and their nanocomposites have been employed as label and/or biosensing transducers to construct electrochemical biosensors for cost-effective detection of toxins with high sensitivity and specificity. This is because the 2D nanomaterials have good electrical conductivity and a large surface area with plenty of active groups for conjugating 2D nanomaterials with the antibodies and/or aptamers of the targeted toxins. Herein, we summarize recent developments in the application of 2D nanomaterial-based electrochemical biosensors for detecting toxins with a particular focus on microbial toxins including bacterial toxins, fungal toxins and algal toxins. The integration of 2D nanomaterials with some existing antibody/aptamer technologies into electrochemical biosensors has led to an unprecedented impact on improving the assaying performance of microbial toxins, and has shown great promise in public health and environmental protection.
Zhuheng Li; Xiaotong Li; Minghong Jian; Girma Selale Geleta; Zhenxin Wang. Two-Dimensional Layered Nanomaterial-Based Electrochemical Biosensors for Detecting Microbial Toxins. Toxins 2019, 12, 20 .
AMA StyleZhuheng Li, Xiaotong Li, Minghong Jian, Girma Selale Geleta, Zhenxin Wang. Two-Dimensional Layered Nanomaterial-Based Electrochemical Biosensors for Detecting Microbial Toxins. Toxins. 2019; 12 (1):20.
Chicago/Turabian StyleZhuheng Li; Xiaotong Li; Minghong Jian; Girma Selale Geleta; Zhenxin Wang. 2019. "Two-Dimensional Layered Nanomaterial-Based Electrochemical Biosensors for Detecting Microbial Toxins." Toxins 12, no. 1: 20.
One-pot solvothermal strategy to synthesize PAA-functionalized porous BiF3:Ln3+ nanospheres towards CT imaging-guided and pH-responsive drug delivery.
Shuang Zhao; Rongrong Tian; Baiqi Shao; Yang Feng; Sen-Wen Yuan; Langping Dong; Liang Zhang; Zhenxin Wang; Hongpeng You. One-pot synthesis of Ln3+-doped porous [email protected] nanospheres for temperature sensing and pH-responsive drug delivery guided by CT imaging. Nanoscale 2019, 12, 695 -702.
AMA StyleShuang Zhao, Rongrong Tian, Baiqi Shao, Yang Feng, Sen-Wen Yuan, Langping Dong, Liang Zhang, Zhenxin Wang, Hongpeng You. One-pot synthesis of Ln3+-doped porous [email protected] nanospheres for temperature sensing and pH-responsive drug delivery guided by CT imaging. Nanoscale. 2019; 12 (2):695-702.
Chicago/Turabian StyleShuang Zhao; Rongrong Tian; Baiqi Shao; Yang Feng; Sen-Wen Yuan; Langping Dong; Liang Zhang; Zhenxin Wang; Hongpeng You. 2019. "One-pot synthesis of Ln3+-doped porous [email protected] nanospheres for temperature sensing and pH-responsive drug delivery guided by CT imaging." Nanoscale 12, no. 2: 695-702.
This review highlights the recent advances in nanomaterial-based immunoassays for MMP abundance measurements and nanomaterial-based biosensors for MMP activity determination.
Zhen Lei; Minghong Jian; Xiaotong Li; Jia Wei; Xianying Meng; Zhenxin Wang. Biosensors and bioassays for determination of matrix metalloproteinases: state of the art and recent advances. Journal of Materials Chemistry B 2019, 8, 3261 -3291.
AMA StyleZhen Lei, Minghong Jian, Xiaotong Li, Jia Wei, Xianying Meng, Zhenxin Wang. Biosensors and bioassays for determination of matrix metalloproteinases: state of the art and recent advances. Journal of Materials Chemistry B. 2019; 8 (16):3261-3291.
Chicago/Turabian StyleZhen Lei; Minghong Jian; Xiaotong Li; Jia Wei; Xianying Meng; Zhenxin Wang. 2019. "Biosensors and bioassays for determination of matrix metalloproteinases: state of the art and recent advances." Journal of Materials Chemistry B 8, no. 16: 3261-3291.
Non-invasive theranostics that integrate the advantages of multimodality imaging and therapeutics have great potential in the field of biomedicine. Herein, a new nanohybrid based on Bi2 Se3 -conjugated upconversion nanoparticles (UCNPs) has been successfully developed through a simple in situ growth strategy. Under 808 nm near-infrared laser irradiation, the UCNPs can emit bright visible light, whereas the Bi2 Se3 nanomaterial exhibits efficient photothermal conversion capacity. Moreover, the as-synthesized UCNP-Bi2 Se3 nanohybrid exhibits efficient cell upconversion luminescence (UCL), reasonable CT imaging, and admirable cancer cell ablation capacity, further emphasizing the efficiency of this strategy for simultaneous UCL imaging and photothermal therapy. The designed theranostic strategy guided by dual-modal imaging endowed with real-time dynamic monitoring, remote controllability, and non-invasiveness makes the UCNP-Bi2 Se3 nanohybrid an ideal candidate for non-invasive multimodal imaging-guided photothermal therapy for the precise diagnosis and treatment of cancer.
Shuang Zhao; Rongrong Tian; Baiqi Shao; Yang Feng; Senwen Yuan; Langping Dong; Liang Zhang; Zhenxin Wang; Hongpeng You. UCNP–Bi 2 Se 3 Upconverting Nanohybrid for Upconversion Luminescence and CT Imaging and Photothermal Therapy. Chemistry – A European Journal 2019, 26, 1127 -1135.
AMA StyleShuang Zhao, Rongrong Tian, Baiqi Shao, Yang Feng, Senwen Yuan, Langping Dong, Liang Zhang, Zhenxin Wang, Hongpeng You. UCNP–Bi 2 Se 3 Upconverting Nanohybrid for Upconversion Luminescence and CT Imaging and Photothermal Therapy. Chemistry – A European Journal. 2019; 26 (5):1127-1135.
Chicago/Turabian StyleShuang Zhao; Rongrong Tian; Baiqi Shao; Yang Feng; Senwen Yuan; Langping Dong; Liang Zhang; Zhenxin Wang; Hongpeng You. 2019. "UCNP–Bi 2 Se 3 Upconverting Nanohybrid for Upconversion Luminescence and CT Imaging and Photothermal Therapy." Chemistry – A European Journal 26, no. 5: 1127-1135.
Matrix metalloproteinases (MMPs) are important biomarkers of various tumors. Herein, a peptide microarray-based fluorescence assay is developed for quantitatively profiling of MMP-2 activity in different matrices through the binding of the immobilized biotinylated peptides on the microarray with the fluorescein isothiocyanate (FITC) modified neutravidins. In the presence of MMP-2, the biotin moiety is released from microarray by enzymatic cleavage of peptide substrate, resulting in the decrease of fluorescence signal. The change of fluorescence intensity is correlated with MMP-2 activity. The detection limit down to 14 pg mL−1 in buffer solution is obtained by a selected peptide substrate for MMP-2 from eleven peptide substrate candidates. The cellular secreted MMP-2 activity levels of different living cells are further successfully quantitatively evaluated by the selected peptide substrate. Using mouse-bearing MG-63 human osteosarcoma as a model system, we also demonstrate that the activity of MMP-2 in serum is closely related with the cancer progression.
Minghong Jian; Min Su; Jiaxue Gao; Zhenxin Wang. Peptide microarray-based fluorescence assay for quantitatively monitoring the tumor-associated matrix metalloproteinase-2 activity. Sensors and Actuators B: Chemical 2019, 304, 127320 .
AMA StyleMinghong Jian, Min Su, Jiaxue Gao, Zhenxin Wang. Peptide microarray-based fluorescence assay for quantitatively monitoring the tumor-associated matrix metalloproteinase-2 activity. Sensors and Actuators B: Chemical. 2019; 304 ():127320.
Chicago/Turabian StyleMinghong Jian; Min Su; Jiaxue Gao; Zhenxin Wang. 2019. "Peptide microarray-based fluorescence assay for quantitatively monitoring the tumor-associated matrix metalloproteinase-2 activity." Sensors and Actuators B: Chemical 304, no. : 127320.
The integration of two-dimensional (2D) nanosheets with biocompatible photothermal nanoparticles may produce effective multifunctional nanotheranostic agents.
Yanhong Sun; Hongda Chen; Guifeng Liu; Lina Ma; Zhenxin Wang. The controllable growth of ultrathin MnO2 on polydopamine nanospheres as a single nanoplatform for the MRI-guided synergistic therapy of tumors. Journal of Materials Chemistry B 2019, 7, 7152 -7161.
AMA StyleYanhong Sun, Hongda Chen, Guifeng Liu, Lina Ma, Zhenxin Wang. The controllable growth of ultrathin MnO2 on polydopamine nanospheres as a single nanoplatform for the MRI-guided synergistic therapy of tumors. Journal of Materials Chemistry B. 2019; 7 (45):7152-7161.
Chicago/Turabian StyleYanhong Sun; Hongda Chen; Guifeng Liu; Lina Ma; Zhenxin Wang. 2019. "The controllable growth of ultrathin MnO2 on polydopamine nanospheres as a single nanoplatform for the MRI-guided synergistic therapy of tumors." Journal of Materials Chemistry B 7, no. 45: 7152-7161.
Yu Fu; Xiaodong Li; Hongda Chen; Zhenxin Wang; Wensheng Yang; Huimao Zhang. CXC Chemokine Receptor 4 Antagonist Functionalized Renal Clearable Manganese-Doped Iron Oxide Nanoparticles for Active-Tumor-Targeting Magnetic Resonance Imaging-Guided Bio-Photothermal Therapy. ACS Applied Bio Materials 2019, 2, 3613 -3621.
AMA StyleYu Fu, Xiaodong Li, Hongda Chen, Zhenxin Wang, Wensheng Yang, Huimao Zhang. CXC Chemokine Receptor 4 Antagonist Functionalized Renal Clearable Manganese-Doped Iron Oxide Nanoparticles for Active-Tumor-Targeting Magnetic Resonance Imaging-Guided Bio-Photothermal Therapy. ACS Applied Bio Materials. 2019; 2 (8):3613-3621.
Chicago/Turabian StyleYu Fu; Xiaodong Li; Hongda Chen; Zhenxin Wang; Wensheng Yang; Huimao Zhang. 2019. "CXC Chemokine Receptor 4 Antagonist Functionalized Renal Clearable Manganese-Doped Iron Oxide Nanoparticles for Active-Tumor-Targeting Magnetic Resonance Imaging-Guided Bio-Photothermal Therapy." ACS Applied Bio Materials 2, no. 8: 3613-3621.
Herein, we rationally fabricated three-dimensional upconversion core–double shell nanodendrites as efficient and safe luminescent probes for in vitro and in vivo bioimaging.
Murad M. A. Abualrejal; Kamel Eid; Rongrong Tian; Lin Liu; Hongda Chen; Aboubakr M. Abdullah; Zhenxin Wang. Rational synthesis of three-dimensional core–double shell upconversion nanodendrites with ultrabright luminescence for bioimaging application. Chemical Science 2019, 10, 7591 -7599.
AMA StyleMurad M. A. Abualrejal, Kamel Eid, Rongrong Tian, Lin Liu, Hongda Chen, Aboubakr M. Abdullah, Zhenxin Wang. Rational synthesis of three-dimensional core–double shell upconversion nanodendrites with ultrabright luminescence for bioimaging application. Chemical Science. 2019; 10 (32):7591-7599.
Chicago/Turabian StyleMurad M. A. Abualrejal; Kamel Eid; Rongrong Tian; Lin Liu; Hongda Chen; Aboubakr M. Abdullah; Zhenxin Wang. 2019. "Rational synthesis of three-dimensional core–double shell upconversion nanodendrites with ultrabright luminescence for bioimaging application." Chemical Science 10, no. 32: 7591-7599.
Herein, an array-based in situ fluorescence assay is proposed for high-throughput analysis and localization of multiplex matrix metalloproteinases (MMPs) activities in cell monolayers and tissue sections. Five specific MMPs (MMP-2, -3, -7, -9, and -14) peptide substrates containing FAM/Dabcyl fluorescent resonance energy transfer (FRET) pair are directly spotted on the surface of cell monolayers or tissue sections, and hydrolyzed by localized MMPs, resulting in fluorescence recovery of FAM. MMPs activities are determined by the fluorescence intensity of stained cells/tissues due to the cellular internalization of peptide fragments with FAM moiety. We demonstrate that the array-based in situ fluorescence assay is suitable for identifying the MMPs expression patterns of cells, as well as determining the secreted MMPs activities in cell monolayer with high sensitivity (as low as hundreds of cells per square centimeter). The feasibility of the assay is further confirmed by evaluating inhibition potencies of six compounds toward five MMPs. Profiling of five MMPs activities in the localized parts of 32 thyroid tissues is performed without separation or extraction procedures, demonstrating the good practicality of the method.
Zhen Lei; Minghong Jian; Jia Wei; Yaoqi Wang; Xianying Meng; Zhenxin Wang. Array-based in situ fluorescence assay for profiling multiplex matrix metalloproteinases activities in tissue section. Analytica Chimica Acta 2019, 1078, 112 -118.
AMA StyleZhen Lei, Minghong Jian, Jia Wei, Yaoqi Wang, Xianying Meng, Zhenxin Wang. Array-based in situ fluorescence assay for profiling multiplex matrix metalloproteinases activities in tissue section. Analytica Chimica Acta. 2019; 1078 ():112-118.
Chicago/Turabian StyleZhen Lei; Minghong Jian; Jia Wei; Yaoqi Wang; Xianying Meng; Zhenxin Wang. 2019. "Array-based in situ fluorescence assay for profiling multiplex matrix metalloproteinases activities in tissue section." Analytica Chimica Acta 1078, no. : 112-118.
[email protected] nanoparticles have been used as a T1-weighted MR contrast agent with active-tumor targeting and antitumor ability.
Yixin Chen; Yu Fu; Xiaodong Li; Hongda Chen; Zhenxin Wang; Huimao Zhang. Peptide-functionalized NaGdF4 nanoparticles for tumor-targeted magnetic resonance imaging and effective therapy. RSC Advances 2019, 9, 17093 -17100.
AMA StyleYixin Chen, Yu Fu, Xiaodong Li, Hongda Chen, Zhenxin Wang, Huimao Zhang. Peptide-functionalized NaGdF4 nanoparticles for tumor-targeted magnetic resonance imaging and effective therapy. RSC Advances. 2019; 9 (30):17093-17100.
Chicago/Turabian StyleYixin Chen; Yu Fu; Xiaodong Li; Hongda Chen; Zhenxin Wang; Huimao Zhang. 2019. "Peptide-functionalized NaGdF4 nanoparticles for tumor-targeted magnetic resonance imaging and effective therapy." RSC Advances 9, no. 30: 17093-17100.
Lanthanide-doped upconversion nanoparticles (UCNPs)-based active targeting optical bioimaging has attracted tremendous scientific interest because of its noninvasive real-time signal feedback, superior tissue penetration depth and high spatial resolution in early diagnosis of disease. Herein, we synthesize a novel carboxy-terminated silica coated NaErF4: 10% [email protected]: 40% [email protected]: 10% [email protected]: 20% Yb UCNPs (termed as [email protected]) with 808 nm near-infrared (NIR) excitation and bright 655 nm upconversion luminescence (UCL) emission for realizing deep tissue imaging. Under 808 nm NIR laser excitation (1.5 W cm−2), the UCL of [email protected] with relative low concentration (2 mg mL−1) can be successfully visualized under a chicken breast slice with 10 mm thickness. After conjugated with various molecules including NH2-PEG3400-COOH, peptide D-SP5 and Uelx Europaeus Agglutinin-I (UEA-I), biodistributions, clearance pathways and tumor-targeting capacities of the [email protected] and corresponding bioconjugates (termed as [email protected], [email protected] and [email protected], respectively) were investigated by tracking the UCL intensities of livers, kidneys and tumors. Both of in vitro and in vivo experimental results reveal that there is no significant difference for their in vivo biodistributions and clearance pathways. The [email protected] exhibits much higher SW480 tumor-targeting capacity than those of other bioconjugates. In particular, the as-prepared [email protected] even to visualize ultrasmall (c.a. 3 mm3 in volume) subcutaneous SW480 tumor in Balb/c nude mouse through intravenous administration. The study implies that the red UCL emitted UCNPs with a minimized heating effect is suitable for deep tissue biomedical imaging and [email protected] can serve as an efficient optical probe for early diagnosis of SW480 tumor.
Rongrong Tian; Shuang Zhao; Guifeng Liu; Hongda Chen; Lina Ma; Hongpeng You; Chunming Liu; Zhenxin Wang. Construction of lanthanide-doped upconversion nanoparticle-Uelx Europaeus Agglutinin-I bioconjugates with brightness red emission for ultrasensitive in vivo imaging of colorectal tumor. Biomaterials 2019, 212, 64 -72.
AMA StyleRongrong Tian, Shuang Zhao, Guifeng Liu, Hongda Chen, Lina Ma, Hongpeng You, Chunming Liu, Zhenxin Wang. Construction of lanthanide-doped upconversion nanoparticle-Uelx Europaeus Agglutinin-I bioconjugates with brightness red emission for ultrasensitive in vivo imaging of colorectal tumor. Biomaterials. 2019; 212 ():64-72.
Chicago/Turabian StyleRongrong Tian; Shuang Zhao; Guifeng Liu; Hongda Chen; Lina Ma; Hongpeng You; Chunming Liu; Zhenxin Wang. 2019. "Construction of lanthanide-doped upconversion nanoparticle-Uelx Europaeus Agglutinin-I bioconjugates with brightness red emission for ultrasensitive in vivo imaging of colorectal tumor." Biomaterials 212, no. : 64-72.
Herein, a novel multifunctional nanoplatform was designed towards multimodality imaging and photothermal therapy (PTT). It was found that Bi nanoparticles could grow in situ on the surface of NaYF4:20%Yb,2%[email protected]:40%[email protected] core-shell nanoparticles (labeled as UCNPs). In this structure, UCNPs were mainly employed as upconversion luminescence (UCL) imaging agent, while the Bi nanoparticles worked as effective CT imaging and photothermal agent. Importantly, a dense SiO2 shell was employed to protect the Bi nanoparticles from oxidation, and it also endowed the nanoplatform with excellent hydrophilic ability. The effective UCL/CT imaging and PTT performance were emphasized by a series of in vivo experiments, which suggest that the integrated nanoplatform with imaging and therapy functions show great promise in the biomedical field.
Shuang Zhao; Rongrong Tian; Baiqi Shao; Yang Feng; Senwen Yuan; Langping Dong; Liang Zhang; Kai Liu; Zhenxin Wang; Hong-Peng You. Designing of [email protected]@SiO2 Hybrid Theranostic Nanoplatforms for Simultaneous Multimodal Imaging and Photothermal Therapy. ACS Applied Materials & Interfaces 2018, 11, 394 -402.
AMA StyleShuang Zhao, Rongrong Tian, Baiqi Shao, Yang Feng, Senwen Yuan, Langping Dong, Liang Zhang, Kai Liu, Zhenxin Wang, Hong-Peng You. Designing of [email protected]@SiO2 Hybrid Theranostic Nanoplatforms for Simultaneous Multimodal Imaging and Photothermal Therapy. ACS Applied Materials & Interfaces. 2018; 11 (1):394-402.
Chicago/Turabian StyleShuang Zhao; Rongrong Tian; Baiqi Shao; Yang Feng; Senwen Yuan; Langping Dong; Liang Zhang; Kai Liu; Zhenxin Wang; Hong-Peng You. 2018. "Designing of [email protected]@SiO2 Hybrid Theranostic Nanoplatforms for Simultaneous Multimodal Imaging and Photothermal Therapy." ACS Applied Materials & Interfaces 11, no. 1: 394-402.