Introduction: Clopidogrel, which is activated by the CYP2C19 enzyme, is among the drugs for which all major regulatory agencies recommend genetic testing to be performed to identify a patient's CYP2C19 genotype in order to determine the optimal antiplatelet therapeutic scheme. The CYP2C19*2 and CYP2C19*3 variants are loss-of-function alleles, leading to abolished CYP2C19 function and thus have the risk of thrombotic events for carriers of these alleles on standard dosages, while the CYP2C19*17 allele results in CYP2C19 hyperactivity. Aims: Here, we report our findings from a retrospective study to assess whether genotyping for the CYP2C19*2 allele was cost effective for myocardial infarction patients receiving clopidogrel treatment in the Serbian population compared with the nongenotype-guided treatment. Results: We found that 59.3% of the CYP2C19*1/*1 patients had a minor or major bleeding event versus 42.85% of the CYP2C19*1/*2 and *2/*2, while a reinfarction event occurred only in 2.3% of the CYP21C9*1...

This study identifies ankrd1a as a novel modulator of skeletal muscle repair in adult zebrafish. Revealing its dual role in newly forming and intact myofibers near injury, the work highlights ankrd1a’s function in fine-tuning myogenic differentiation during repair. We position ankrd1a as both a marker of myofibers that repopulate wounded tissue and a player in coordination of adaptive responses essential for effective muscle tissue recovery.

In contrast to humans, zebrafish have an outstanding ability to regenerate injured heart through a highly orchestrated process involving all cardiac structures. To replace the lost myocardium, resident cardiomyocytes (CMs) dedifferentiate and proliferate, invading the injured area. The response of the myocardium is preceded by the activation of the epicardium and endocardium, which form active scaffolds to provide mechanical and paracrine support to guide regeneration. New CMs use protrusions to migrate and invade fibrotic injured tissue, replacing it with functional myocardium. Here, we investigated the expression profile of the stress-responsive ankrd1a gene in different cardiac structures, at key time points during regeneration, aiming to gain insight into its precise roles during zebrafish heart regeneration. In the TgBAC(ankrd1a:EGFP) reporter line, transgene upregulation was restricted to the myocardium, initiated as early as 15 h post-cryoinjury, and consistently marked CMs bordering the injury or scar area during regeneration. Transcriptome profiling and immunostaining revealed a potential role of ankrd1a in regulating CMs' dedifferentiation, as well as changes in the expression of genes associated with antigen presentation and extracellular matrix composition in the ankrd1a mutant. Our results indicate that ankrd1a is dispensable for ventricular regeneration after cryoinjury, while it may serve as a marker and fine-tuner in the healing process of injured cardiac muscle.

The increasing demand for natural, safe, and sustainable ingredients is driving innovation in cosmetic science. This study assessed the anti-aging potential of 17 petal extracts using a multidisciplinary bioanalytical approach. In vitro spectrophotometric assays evaluated anti-wrinkle (anti-elastase), anti-pigmentation (anti-tyrosinase), and antioxidant (DPPH, ABTS) activities, while cytotoxicity was tested on HaCaT keratinocytes. Chemical profiling using HPTLC and UHPLC–MS/MS identified 17 phenolic compounds. For the first time, petals from prairie rose (Rosa setigera Michx.), common peony (Paeonia officinalis L.), horse-chestnut cultivars (Aesculus hippocastanum L., Aesculus × carnea Zeyx.), lilac (Syringa vulgaris), mock-orange (Philadelphus pubescens Loisel), orange lily (Lilium bulbiferum L.), garden tulip (Tulipa gesneriana L.), ivy geranium (Pelargonium × peltatum (L.) L’Hér. ex Aiton), and wallflower (Erysimum × cheiri (L.) Crantz) were studied for their skin anti-aging properties. Prairie rose, peony, and ivy geranium extracts showed strong anti-elastase activity; rose and peony also demonstrated high antioxidant potential, while lilac exhibited significant anti-tyrosinase effects. Key phenolic constituents—caffeic acid, p-coumaric acid, and gallic acid—were further examined via molecular docking, which confirmed their inhibitory properties by revealing inhibition mechanisms. All extracts were confirmed to be non-toxic in zebrafish acute toxicity assays at relevant concentrations. This integrative strategy effectively links chemical composition with biological activity, offering valuable insight into the development of safe, plant-derived anti-aging agents for sustainable cosmetic applications.