The successful application of assisted reproductive technologies (ARTs), such as in vitro maturation (IVM) and artificial oocyte activation, requires species-specific adaptations. Although these methods are routinely used in laboratory rodents, their use in wild or non-model species remains limited, such as the Spix’s yellow-toothed cavy, a Neotropical species of ecological and reproductive interest. This study evaluated the effects of different concentrations of epidermal growth factor (EGF; 10 or 50 ng/mL) on IVM (Experiment 1) and of 6-dimethylaminopurine (6-DMAP) on artificial oocyte activation (Experiment 2). EGF at 10 ng/mL (93.8% ± 1.6; 84.9% ± 0.7) promoted greater viability and less apoptosis in cumulus cells, compared to 50 ng/mL (83.0% ± 1.6; 78.9% ± 2.7), maintaining cumulus expansion, ultrastructural integrity, and better morphometric quality of oocytes. Thus, this concentration was used in the next step, where oocytes were activated with or without 6-DMAP. After five days, the presence of 6-DMAP increased cleavage rates (69.3% ± 5.0) compared to activation without the compound (53.5% ± 3.5), without significantly affecting morula formation (13.2% ± 3.1 to 17.3% ± 2.9). It is concluded that EGF improves the oocyte microenvironment, while 6-DMAP enhances cleavage, with these being the initial steps in the development of ARTs for Spix’s yellow-toothed cavy.

The Greater Caribbean manatee (Trichechus manatus manatus, Linnaeus 1758), previously classified as Vulnerable by the International Union for Conservation of Nature (IUCN) worldwide (Deutsch et al....