The pathological agentsToxoplasma gondii, Ancylostoma caninum,andToxocara canisare widely distributed zoonotic parasites with high prevalence in tropical and subtropical regions of the world. The aim of the present study was to determine the presence of DNA from these parasites in sand samples from the sand playgrounds in the southeastern region of Mexico. Samples of sand were collected from 68 playgrounds in public parks in the city of Merida, Yucatan, which is the main urban area in the southeast of Mexico. The samples were examined using nested PCR to detect the SAG1 gene fromToxoplasma gondii, and endpoint PCR for the amplification of ITS-2 and rRNA-ITS2 genes fromToxocara canisandAncylostoma caninum,respectively. The presence ofT. gondiiDNA was detected in 11.8% (8/68) samples, DNA fromA. caninumandT. caniswas not detected. Results indicate that playgrounds from the studied sandboxes are contaminated withT. gondiioocysts and may represent a risk of infection for people in contact with the sand, especially for preschoolers.