Pre-treated beef patties (BPs) face storage instability due to lipid/protein oxidation, microbial spoilage, and quality loss. Purple onion pulp (OP), a bioactive-rich product, offers potential as a natural preservative. This study evaluated the utilization of OP (2.5%, 5.0%, 10.0%) in BPs for refrigerated (4 °C) stability. The results showed that during storage, OP addition reduced the pH value, a* value, and b* value of beef, while cooking loss was not significantly affected. At the end of storage, the addition of 10.0% OP decreased the formation of metmyoglobin (MetMb), thiobarbituric acid reactive substances (TBARSs) (with a reduction rate of 15.96%), and carbonyl groups, and also inhibited spoilage bacteria. Sensory evaluation and texture analysis indicated that the addition of OP improved the hardness, juiciness, and odor of beef. Specifically, the incorporation of 10.0% OP extended the shelf life of BPs to 9 days, effectively improving their storage stability. Therefore, adding 10% purple onion pulp OP to BPs can improve the storage stability and sensory quality of refrigerated BPs.
Heightened consumer awareness of health benefits has propelled nutritionally enhanced cereal products to the forefront of food industry innovation. The present work focused on manufacturing polyphenol-enriched biscuits by incorporating grape seed extract (GSE). The study evaluated different formulations of wheat flour with GSE at addition ratios of 0%, 0.2%, 0.4%, 0.6%, 0.8%, and 1% (w/w). Within the 0.2–0.6% GSE range, significant improvements were observed in dough properties and biscuit quality. Specifically, the dough development time and degree of softening decreased, while the dough stability time, peak viscosity, storage modulus, and loss modulus increased. The fortified biscuits had a darker color, lower baking loss rate, and increased spread factor, springiness, and cohesiveness. In addition, total phenolic content (TPC) and total flavonoid content (TFC) of the fortified biscuits increased significantly (p < 0.05), and the antioxidant activity of the biscuits was also enhanced. Sensory evaluation results indicated that biscuits with 0.6% GSE addition achieved the highest overall acceptability score (8.7 ± 0.4 on a 9-point hedonic scale), indicating good consumer acceptance. These findings identify 0.6% GSE as an appropriate fortification level for improving biscuit quality while enhancing polyphenol content and antioxidant capacity, providing a practical basis for the development of polyphenol-enriched bakery products.
The increasing demand for functional products has promoted research on bioactive compounds. Dandelion root polysaccharide (DRP), a key bioactive component of Taraxacum officinale, has anti-inflammatory, antioxidant, hypoglycemic and hypolipidemic activities. This study extracted DRP via microwave-assisted enzymatic extraction (MAEE) to obtain mDRP, and isolated acidic polysaccharide mDRP-1 (7.548 kDa) by column chromatography. HPLC showed that both contain six monosaccharides (Man, Rha, GalA, Glu, Gal, Ara) with different molar ratios. mDRP is heterogeneous (trace protein/nucleic acids, no triple helix, amorphous), while mDRP-1 has a triple-helical, semi-crystalline structure; both have characteristic polysaccharide peaks and pyranose rings, and rough, porous surfaces. mDRP had better thermal stability and significant concentration-dependent in vitro antioxidant and hypoglycaemic effects.