This article presents the results of the Innovation Support Program (ISP), designed to enhance the market readiness of 12 bio-based innovators from six European rural regions: Northern Sweden, Mazovia (Poland), Upper Austria, Pays de la Loire (France), Strumica (Macedonia), and Andalusia (Spain). Over three years, the ISP applied a modular and flexible methodology, beginning with a cross-regional needs analysis to identify knowledge gaps, followed by a call for Expressions of Interest to select promising bio-based solutions, and concluding with tailored support delivered through regional Task Forces. These provided mentoring and capacity-building activities focusing on business modeling, market analysis, and funding opportunities. The program identified market access as a major barrier to scaling up and noted that many solutions followed Social and Solidarity Economy principles, prioritizing social and environmental impact over profit. Through targeted assistance and knowledge exchange, the ISP strengthened local innovation capacity and contributed measurable progress in companies’ Technology Readiness Levels (TRLs) and Key Performance Indicators (KPIs). Positioned within the framework of the EU Bioeconomy Strategy, the ISP demonstrates how combining regional insights with a structured support framework can effectively accelerate the scaling of bio-based solutions, highlighting the need for iterative, long-term support to sustain regional bioeconomy growth.