Different techniques have been developed for the remediation of Cu contaminated soils, being the phytoremediation a sustainable and environmentally friendly strategy, but its use in mine tailings is scarce. Arbuscular mycorrhizal fungi (AMF) can decrease the Cu concentration in plants by favouring the stabilization of this metal through different mechanisms such as the production of glomalin, immobilization in the fungal wall of hyphae and spores, and the storage of Cu in vacuoles. Additionally, the use of organic amendments promotes the beneficial effects produced by AMF and improves plant growth. Based on the above, the aim of this study was to determine the effect of AMF inoculation and compost application at different doses on the growth of Oenothera picensis in a Cu mine tailing. One group of plants were inoculated with Claroideoglomus claroideum (CC) and other was non-inoculated (NM). Both CC and NM were grown for two month under greenhouse conditions in pots with the Cu mine tailing, which also had increasing compost doses (0%, 2.5%, 5%, and 10%). Results showed greater biomass production of O. picensis by CC up to 2-fold compared with NM. This effect was improved by the compost addition, especially at doses of 5% and 10%. Therefore, the increase of mycorrhizal and nutritional parameters in O. picensis, and the decreasing of Cu availability in the mine tailing, promoted the production of photosynthetic pigments together with the plant growth, which is of importance to accomplish phytoremediation programs in Cu mine tailings.

The aim of this study was to estimate both the contribution of drinking water and food (raw and cooked) to the total (t-As) and inorganic (i-As) arsenic intake and the exposure of inhabitants of Socaire, a rural village in Chile´s Antofagasta Region, by using urine as biomarker. The i-As intake from food and water was estimated using samples collected between November 2008 and September 2009. A 24-hour dietary recall questionnaire was given to 20 participants. Drinking water, food (raw and cooked) and urine samples were collected directly from the homes where the interviewees lived. The percentage of i-As/t-As in the drinking water that contributed to the total intake was variable (26.8–92.9). Cereals and vegetables are the food groups that contain higher concentrations of i-As. All of the participants interviewed exceeded the reference intake FAO/OMS (149.8 µg∙i-As·day−1) by approximately nine times. The concentration of t-As in urine in each individual ranged from 78 to 459 ng·mL−1. Estimated As intake from drinking water and food was not associated with total urinary As concentration. The results show that both drinking water and food substantially contribute to i-As intake and an increased exposure risk to adult residents in contaminated areas.