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Pengfei Qi
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, China

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Article
Published: 17 August 2021 in Molecular Breeding
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Wheat is a major staple food crop worldwide because of the unique properties of wheat flour. High molecular weight glutenin subunits (HMW-GSs), which are among the most critical determinants of wheat flour quality, are responsible for the formation of glutenin polymeric structures via interchain disulfide bonds. We herein describe the identification of a new HMW-GS Dy10 allele (Dy10-m619SN). The amino acid substitution (serine-to-asparagine) encoded in this allele resulted in a partial post-translational cleavage that produced two new peptides. These new peptides disrupted the interactions among gluten proteins because of the associated changes to the number of available cysteine residues for interchain disulfide bonds. Consequently, Dy10-m619SN expression decreased the size of glutenin polymers and weakened glutens, which resulted in wheat dough with improved cookie-making quality, without changes to the glutenin-to-gliadin ratio. In this study, we clarified the post-translational processing of HMW-GSs and revealed a new genetic resource useful for wheat breeding.

ACS Style

Yan Wang; Qing Chen; Yang Li; Zhenru Guo; Caihong Liu; Yongfang Wan; Malcolm Hawkesford; Jing Zhu; Wang Wu; Meiqiao Wei; Kan Zhao; Yunfeng Jiang; Yazhou Zhang; Qiang Xu; Li Kong; Zhien Pu; Mei Deng; Qiantao Jiang; Xiujin Lan; Jirui Wang; Guoyue Chen; Jian Ma; Youliang Zheng; Yuming Wei; Pengfei Qi. Post-translational cleavage of HMW-GS Dy10 allele improves the cookie-making quality in common wheat (Triticum aestivum). Molecular Breeding 2021, 41, 1 -15.

AMA Style

Yan Wang, Qing Chen, Yang Li, Zhenru Guo, Caihong Liu, Yongfang Wan, Malcolm Hawkesford, Jing Zhu, Wang Wu, Meiqiao Wei, Kan Zhao, Yunfeng Jiang, Yazhou Zhang, Qiang Xu, Li Kong, Zhien Pu, Mei Deng, Qiantao Jiang, Xiujin Lan, Jirui Wang, Guoyue Chen, Jian Ma, Youliang Zheng, Yuming Wei, Pengfei Qi. Post-translational cleavage of HMW-GS Dy10 allele improves the cookie-making quality in common wheat (Triticum aestivum). Molecular Breeding. 2021; 41 (8):1-15.

Chicago/Turabian Style

Yan Wang; Qing Chen; Yang Li; Zhenru Guo; Caihong Liu; Yongfang Wan; Malcolm Hawkesford; Jing Zhu; Wang Wu; Meiqiao Wei; Kan Zhao; Yunfeng Jiang; Yazhou Zhang; Qiang Xu; Li Kong; Zhien Pu; Mei Deng; Qiantao Jiang; Xiujin Lan; Jirui Wang; Guoyue Chen; Jian Ma; Youliang Zheng; Yuming Wei; Pengfei Qi. 2021. "Post-translational cleavage of HMW-GS Dy10 allele improves the cookie-making quality in common wheat (Triticum aestivum)." Molecular Breeding 41, no. 8: 1-15.

Journal article
Published: 07 August 2021 in International Journal of Molecular Sciences
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Wheat is a major staple food crop worldwide, due to its total yield and unique processing quality. Its grain yield and quality are threatened by Fusarium head blight (FHB), which is mainly caused by Fusarium graminearum. Salicylic acid (SA) has a strong and toxic effect on F. graminearum and is a hopeful target for sustainable control of FHB. F. graminearum is capable of efficientdealing with SA stress. However, the underlying mechanisms remain unclear. Here, we characterized FgMFS1 (FGSG_03725), a major facilitator superfamily (MFS) transporter gene in F. graminearum. FgMFS1 was highly expressed during infection and was upregulated by SA. The predicted three-dimensional structure of the FgMFS1 protein was consistent with the schematic for the antiporter. The subcellular localization experiment indicated that FgMFS1 was usually expressed in the vacuole of hyphae, but was alternatively distributed in the cell membrane under SA treatment, indicating an element of F. graminearum in response to SA. ΔFgMFS1 (loss of function mutant of FgMFS1) showed enhanced sensitivity to SA, less pathogenicity towards wheat, and reduced DON production under SA stress. Re-introduction of a functional FgMFS1 gene into ∆FgMFS1 recovered the mutant phenotypes. Wheat spikes inoculated with ΔFgMFS1 accumulated more SA when compared to those inoculated with the wild-type strain. Ecotopic expression of FgMFS1 in yeast enhanced its tolerance to SA as expected, further demonstrating that FgMFS1 functions as an SA exporter. In conclusion, FgMFS1 encodes an SA exporter in F. graminearum, which is critical for its response to wheat endogenous SA and pathogenicity towards wheat.

ACS Style

Qing Chen; Lu Lei; Caihong Liu; Yazhou Zhang; Qiang Xu; Jing Zhu; Zhenru Guo; Yan Wang; Qingcheng Li; Yang Li; Li Kong; Yunfeng Jiang; Xiujin Lan; Jirui Wang; Qiantao Jiang; Guoyue Chen; Jian Ma; Yuming Wei; Youliang Zheng; Pengfei Qi. Major Facilitator Superfamily Transporter Gene FgMFS1 Is Essential for Fusarium graminearum to Deal with Salicylic Acid Stress and for Its Pathogenicity towards Wheat. International Journal of Molecular Sciences 2021, 22, 8497 .

AMA Style

Qing Chen, Lu Lei, Caihong Liu, Yazhou Zhang, Qiang Xu, Jing Zhu, Zhenru Guo, Yan Wang, Qingcheng Li, Yang Li, Li Kong, Yunfeng Jiang, Xiujin Lan, Jirui Wang, Qiantao Jiang, Guoyue Chen, Jian Ma, Yuming Wei, Youliang Zheng, Pengfei Qi. Major Facilitator Superfamily Transporter Gene FgMFS1 Is Essential for Fusarium graminearum to Deal with Salicylic Acid Stress and for Its Pathogenicity towards Wheat. International Journal of Molecular Sciences. 2021; 22 (16):8497.

Chicago/Turabian Style

Qing Chen; Lu Lei; Caihong Liu; Yazhou Zhang; Qiang Xu; Jing Zhu; Zhenru Guo; Yan Wang; Qingcheng Li; Yang Li; Li Kong; Yunfeng Jiang; Xiujin Lan; Jirui Wang; Qiantao Jiang; Guoyue Chen; Jian Ma; Yuming Wei; Youliang Zheng; Pengfei Qi. 2021. "Major Facilitator Superfamily Transporter Gene FgMFS1 Is Essential for Fusarium graminearum to Deal with Salicylic Acid Stress and for Its Pathogenicity towards Wheat." International Journal of Molecular Sciences 22, no. 16: 8497.

Research article
Published: 31 May 2021 in Genome
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The basic leucine zipper (bZIP) family of genes encode transcription factors that play key roles in plant growth and development. In this study, a total of 92 HvbZIP genes were identified and compared with previous studies using recently released barley genome data. Two novel genes were characterized in this study, and some misannotated and duplicated genes from previous studies have been corrected. Phylogenetic analysis results showed that 92 HvbZIP genes were classified into 10 groups and three unknown groups. The gene structure and motif distribution of the three unknown groups implied that the genes of the three groups may be functionally different. Expression profiling indicated that the HvbZIP genes exhibited different patterns of spatial and temporal expression. Using qRT-PCR, more than 10 HvbZIP genes were identified with expression patterns similar to those of starch synthase genes in barley. Yeast one-hybrid analysis revealed that two of the HvbZIP genes exhibited in vitro binding activity to the promoter of HvAGP-S. The two HvbZIP genes may be candidate genes for further study to explore the mechanism by which they regulate the synthesis of barley starch.

ACS Style

Xiaojuan Zhong; Xiuqin Feng; Yulong Li; Carlos Guzmán; Na Lin; Qiang Xu; Yazhou Zhang; Huaping Tang; Pengfei Qi; Mei Deng; Jian Ma; Jirui Wang; Guoyue Chen; Xiujin Lan; Yuming Wei; Youliang Zheng; Qiantao Jiang. Genome-wide identification of bZIP transcription factor genes related to starch synthesis in barley (Hordeum vulgare L.). Genome 2021, 1 -14.

AMA Style

Xiaojuan Zhong, Xiuqin Feng, Yulong Li, Carlos Guzmán, Na Lin, Qiang Xu, Yazhou Zhang, Huaping Tang, Pengfei Qi, Mei Deng, Jian Ma, Jirui Wang, Guoyue Chen, Xiujin Lan, Yuming Wei, Youliang Zheng, Qiantao Jiang. Genome-wide identification of bZIP transcription factor genes related to starch synthesis in barley (Hordeum vulgare L.). Genome. 2021; ():1-14.

Chicago/Turabian Style

Xiaojuan Zhong; Xiuqin Feng; Yulong Li; Carlos Guzmán; Na Lin; Qiang Xu; Yazhou Zhang; Huaping Tang; Pengfei Qi; Mei Deng; Jian Ma; Jirui Wang; Guoyue Chen; Xiujin Lan; Yuming Wei; Youliang Zheng; Qiantao Jiang. 2021. "Genome-wide identification of bZIP transcription factor genes related to starch synthesis in barley (Hordeum vulgare L.)." Genome , no. : 1-14.

Research article
Published: 04 May 2021 in Plant Disease
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Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici, is one of the most destructive diseases of wheat worldwide. Chinese wheat landrace Guangtoumai (GTM) exhibited a high level of resistance against predominant P. striiformis f. sp. tritici races in China at the adult plant stage. The objective of this research was to identify and map the major locus/loci for stripe rust resistance in GTM. A set of 212 recombinant inbred lines (RILs) was developed from a cross between GTM and Avocet S. The parents and RILs were evaluated in three field tests (2018, 2019, and 2020 at Chongzhou, Sichuan) with the currently predominant P. striiformis f. sp. tritici races for final disease severity and genotyped with the Wheat 55K single nucleotide polymorphism (SNP) array to construct a genetic map with 1,031 SNP markers. A major locus, named QYr.GTM-5DL, was detected on chromosome 5DL in GTM. The locus was mapped in a 2.75-cM interval flanked by SNP markers AX-109855976 and AX-109453419, explaining up to 44.4% of the total phenotypic variation. Since no known Yr genes have been reported on chromosome 5DL, QYr.GTM-5DL is very likely a novel adult plant resistance locus. Haplotype analysis revealed that the resistance allele displayed enhanced levels of stripe rust resistance and is likely present in 5.3% of the 247 surveyed Chinese wheat landraces. The derived cleaved amplified polymorphic sequence (dCAPS) marker dCAPS-5722, converted from a SNP marker tightly linked to QYr.GTM-5DL with 0.3 cM, was validated on a subset of RILs and 48 commercial wheat cultivars developed in Sichuan. The results indicated that QYr.GTM-5DL with its linked dCAPS marker could be used in marker-assisted selection to improve stripe rust resistance in breeding programs, and this quantitative trait locus will provide new and possibly durable resistance to stripe rust.

ACS Style

Yu Wu; Yuqi Wang; Fangjie Yao; Li Long; Jing Li; Hao Li; Zhien Pu; Wei Li; Qian-Tao Jiang; Jirui Wang; Yu-Ming Wei; Jian Ma; Houyang Kang; Pengfei Qi; Shoufen Dai; Mei Deng; You-Liang Zheng; Yunfeng Jiang; Guo-Yue Chen. Molecular Mapping of a Novel Quantitative Trait Locus Conferring Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Guangtoumai. Plant Disease 2021, 1 -07.

AMA Style

Yu Wu, Yuqi Wang, Fangjie Yao, Li Long, Jing Li, Hao Li, Zhien Pu, Wei Li, Qian-Tao Jiang, Jirui Wang, Yu-Ming Wei, Jian Ma, Houyang Kang, Pengfei Qi, Shoufen Dai, Mei Deng, You-Liang Zheng, Yunfeng Jiang, Guo-Yue Chen. Molecular Mapping of a Novel Quantitative Trait Locus Conferring Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Guangtoumai. Plant Disease. 2021; ():1-07.

Chicago/Turabian Style

Yu Wu; Yuqi Wang; Fangjie Yao; Li Long; Jing Li; Hao Li; Zhien Pu; Wei Li; Qian-Tao Jiang; Jirui Wang; Yu-Ming Wei; Jian Ma; Houyang Kang; Pengfei Qi; Shoufen Dai; Mei Deng; You-Liang Zheng; Yunfeng Jiang; Guo-Yue Chen. 2021. "Molecular Mapping of a Novel Quantitative Trait Locus Conferring Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Guangtoumai." Plant Disease , no. : 1-07.

Preprint content
Published: 16 March 2021
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Wheat is a major staple food crop worldwide because of the unique properties of wheat flour. High molecular weight glutenin subunits (HMW-GSs), which are among the most critical determinants of wheat flour quality, are responsible for the formation of glutenin polymeric structures via interchain disulfide bonds. We herein describe the identification of a new HMW-GS Dy10 allele (Dy10-m619SN). The amino acid substitution (serine-to-asparagine) encoded in this allele resulted in a partial post-translational cleavage that produced two new peptides. These new peptides disrupted the interactions among gluten proteins because of the associated changes to the number of available cysteine residues for interchain disulfide bonds. Consequently, Dy10-m619SN expression decreased the size of glutenin polymers and weakened glutens, which resulted in wheat dough with improved cookie-making quality, without changes to the glutenin-to-gliadin ratio. In this study, we clarified the post-translational processing of HMW-GSs and revealed a new genetic resource useful for wheat breeding.

ACS Style

Yan Wang; Qing Chen; Yang Li; Zhenru Guo; Caihong Liu; Yongfang Wan; Malcolm Hawkesford; Jing Zhu; Wang Wu; Meiqiao Wei; Kan Zhao; Yunfeng Jiang; Yazhou Zhang; Qiang Xu; Li Kong; Zhien Pu; Mei Deng; Qiantao Jiang; Xiujin Lan; Jirui Wang; Guoyue Chen; Jian Ma; Youliang Zheng; Yuming Wei; Pengfei Qi. Post-Translational Cleavage of HMW-GS Dy10 allele improves the cookie-making quality in common wheat (Triticum aestivum). 2021, 1 .

AMA Style

Yan Wang, Qing Chen, Yang Li, Zhenru Guo, Caihong Liu, Yongfang Wan, Malcolm Hawkesford, Jing Zhu, Wang Wu, Meiqiao Wei, Kan Zhao, Yunfeng Jiang, Yazhou Zhang, Qiang Xu, Li Kong, Zhien Pu, Mei Deng, Qiantao Jiang, Xiujin Lan, Jirui Wang, Guoyue Chen, Jian Ma, Youliang Zheng, Yuming Wei, Pengfei Qi. Post-Translational Cleavage of HMW-GS Dy10 allele improves the cookie-making quality in common wheat (Triticum aestivum). . 2021; ():1.

Chicago/Turabian Style

Yan Wang; Qing Chen; Yang Li; Zhenru Guo; Caihong Liu; Yongfang Wan; Malcolm Hawkesford; Jing Zhu; Wang Wu; Meiqiao Wei; Kan Zhao; Yunfeng Jiang; Yazhou Zhang; Qiang Xu; Li Kong; Zhien Pu; Mei Deng; Qiantao Jiang; Xiujin Lan; Jirui Wang; Guoyue Chen; Jian Ma; Youliang Zheng; Yuming Wei; Pengfei Qi. 2021. "Post-Translational Cleavage of HMW-GS Dy10 allele improves the cookie-making quality in common wheat (Triticum aestivum)." , no. : 1.

Regular article
Published: 02 March 2021 in New Phytologist
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Pre‐harvest sprouting (PHS), the germination of grain before harvest, is a serious problem resulting in wheat yield and quality losses. Here, we mapped the PHS resistance gene PHS‐3D from synthetic hexaploid wheat to a 2.4 Mb presence‐absence variation (PAV) region and found that its resistance effect was attributed to the pleiotropic Myb10‐D by integrated omics and functional analyses. Three haplotypes were detected in this PAV region among 262 worldwide wheat lines and 16 Aegilops tauschii, and the germination percentages of wheat lines containing Myb10‐D was approximately 40% lower than that of the other lines. Transcriptome and metabolome profiling indicated that Myb10‐D affected the transcription of genes in both the flavonoid and ABA biosynthesis pathways, which resulted in increases in flavonoids and ABA in transgenic wheat lines. Myb10‐D activates NCED by biding the secondary wall MYB‐responsive element (SMRE) to promote ABA biosynthesis in early wheat seed development stages. We revealed that the newly discovered function of Myb10‐D confers PHS resistance by enhancing ABA biosynthesis to delay germination in wheat. The PAV harboring Myb10‐D associated with grain color and PHS will be useful for understanding and selecting white grained PHS resistant wheat cultivars.

ACS Style

Jing Lang; Yuxin Fu; Yong Zhou; Mengping Cheng; Min Deng; Maolian Li; Tingting Zhu; Jian Yang; Xiaojiang Guo; Lixuan Gui; Linchuan Li; Zhongxu Chen; Yingjin Yi; Lianquan Zhang; Ming Hao; Lin Huang; Chao Tan; Guoyue Chen; Qiantao Jiang; Pengfei Qi; Zhien Pu; Jian Ma; Zehou Liu; Yujiao Liu; Ming‐Cheng Luo; Yuming Wei; Youliang Zheng; Yongrui Wu; Dengcai Liu; Jirui Wang. Myb10‐D confers PHS‐3D resistance to pre‐harvest sprouting by regulating NCED in ABA biosynthesis pathway of wheat. New Phytologist 2021, 230, 1940 -1952.

AMA Style

Jing Lang, Yuxin Fu, Yong Zhou, Mengping Cheng, Min Deng, Maolian Li, Tingting Zhu, Jian Yang, Xiaojiang Guo, Lixuan Gui, Linchuan Li, Zhongxu Chen, Yingjin Yi, Lianquan Zhang, Ming Hao, Lin Huang, Chao Tan, Guoyue Chen, Qiantao Jiang, Pengfei Qi, Zhien Pu, Jian Ma, Zehou Liu, Yujiao Liu, Ming‐Cheng Luo, Yuming Wei, Youliang Zheng, Yongrui Wu, Dengcai Liu, Jirui Wang. Myb10‐D confers PHS‐3D resistance to pre‐harvest sprouting by regulating NCED in ABA biosynthesis pathway of wheat. New Phytologist. 2021; 230 (5):1940-1952.

Chicago/Turabian Style

Jing Lang; Yuxin Fu; Yong Zhou; Mengping Cheng; Min Deng; Maolian Li; Tingting Zhu; Jian Yang; Xiaojiang Guo; Lixuan Gui; Linchuan Li; Zhongxu Chen; Yingjin Yi; Lianquan Zhang; Ming Hao; Lin Huang; Chao Tan; Guoyue Chen; Qiantao Jiang; Pengfei Qi; Zhien Pu; Jian Ma; Zehou Liu; Yujiao Liu; Ming‐Cheng Luo; Yuming Wei; Youliang Zheng; Yongrui Wu; Dengcai Liu; Jirui Wang. 2021. "Myb10‐D confers PHS‐3D resistance to pre‐harvest sprouting by regulating NCED in ABA biosynthesis pathway of wheat." New Phytologist 230, no. 5: 1940-1952.

Research article
Published: 18 February 2021 in Phytopathology®
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Chinese wheat landrace Anyuehong (AYH) has displayed high levels of stable adult-plant resistance (APR) to stripe rust for more than 15 years. To identify QTL for stripe rust resistance in AYH, a set of 110 recombinant inbred lines (RILs) was developed from a cross between AYH and susceptible cultivar Taichung 29. The parents and RILs were evaluated for final disease severity (FDS) in six field tests with a mixture of predominant Pst races at the adult-plant stage and genotyped using the wheat 55K SNP array to construct a genetic map with 1,143 SNP markers. Three QTL, designated as QYr.AYH-1AS, QYr.AYH-5BL and QYr.AYH-7DS, were mapped on chromosome 1AS, 5BL and 7DS, respectively. RILs combining three QTL showed significantly reduced FDS compared with the lines in other combinations. Of them, QYr.AYH-5BL and QYr.AYH-7DS were stably detected in all environments, explaining 13.6–21.4% and 17.6–33.6% of phenotypic variation, respectively. Compared with previous studies, QYr.AYH-5BL may be a new QTL, while QYr.AYH-7DS may be Yr18. Haplotype analysis revealed that QYr.AYH-5BL is likely present in 6.2% of the 323 surveyed Chinese wheat landraces. The Kompetitive allele specific PCR (KASP) markers for QYr.AYH-5BL were developed by the linked SNP markers to successfully confirm the effects of the QTL in a validation population derived from a residual heterozygous line, and were further assessed in 38 Chinese wheat landraces and 92 cultivars. Our results indicated that QYr.AYH-5BL with linked KASP markers should have potential value for marker-assisted selection to improve stripe rust resistance in breeding programs.

ACS Style

Li Long; Fangjie Yao; Miss Fangnian Guan; Yu-Kun Cheng; Miss Luyao Duan; Xuyang Zhao; Hao Li; Zhien Pu; Wei Li; Qian-Tao Jiang; Yuming Wei; Jian Ma; Houyang Kang; Shoufen Dai; Pengfei Qi; Qiang Xu; Mei Deng; You-Liang Zheng; Yunfeng Jiang; Guoyue Chen. A stable QTL on Chromosome 5BL Combined with Yr18 Conferring High-level Adult-plant Resistance to Stripe Rust in Chinese Wheat Landrace Anyuehong. Phytopathology® 2021, 1 .

AMA Style

Li Long, Fangjie Yao, Miss Fangnian Guan, Yu-Kun Cheng, Miss Luyao Duan, Xuyang Zhao, Hao Li, Zhien Pu, Wei Li, Qian-Tao Jiang, Yuming Wei, Jian Ma, Houyang Kang, Shoufen Dai, Pengfei Qi, Qiang Xu, Mei Deng, You-Liang Zheng, Yunfeng Jiang, Guoyue Chen. A stable QTL on Chromosome 5BL Combined with Yr18 Conferring High-level Adult-plant Resistance to Stripe Rust in Chinese Wheat Landrace Anyuehong. Phytopathology®. 2021; ():1.

Chicago/Turabian Style

Li Long; Fangjie Yao; Miss Fangnian Guan; Yu-Kun Cheng; Miss Luyao Duan; Xuyang Zhao; Hao Li; Zhien Pu; Wei Li; Qian-Tao Jiang; Yuming Wei; Jian Ma; Houyang Kang; Shoufen Dai; Pengfei Qi; Qiang Xu; Mei Deng; You-Liang Zheng; Yunfeng Jiang; Guoyue Chen. 2021. "A stable QTL on Chromosome 5BL Combined with Yr18 Conferring High-level Adult-plant Resistance to Stripe Rust in Chinese Wheat Landrace Anyuehong." Phytopathology® , no. : 1.

Journal article
Published: 01 January 2020 in Genome
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A completely developed and vigorous root system can provide a stable platform for aboveground plant organs. To identify loci controlling root traits that could be used in wheat (Triticum aestivum L.) breeding, 199 recombinant inbred lines were used to measure and analyze eight root traits. A total of 18 quantitative trait loci (QTL) located on chromosomes 1A, 2A, 2B, 2D, 4B, 4D, 6A, 7A, and 7B were identified. The phenotypic variation explained by these 18 QTL ranged from 3.27% to 11.75%, and the logarithm of odds scores ranged from 2.50 to 6.58. A comparison of physical intervals indicated several new QTL for root traits were identified. In addition, significant correlations between root and agronomic traits were detected and discussed. The results presented in this study, along with those of previous reports, suggest that chromosomes 2 and 7 likely play important roles in the growth and development of wheat roots.

ACS Style

Ting Li; Jian Ma; Yaya Zou; Guangdeng Chen; Pu-Yang Ding; Han Zhang; Cong-Cong Yang; Yang Mu; Huaping Tang; Yaxi Liu; Qiantao Jiang; Guo-Yue Chen; Peng-Fei Qi; Yu-Ming Wei; Youliang Zheng; Xiu-Jin Lan. Quantitative trait loci for seeding root traits and the relationships between root and agronomic traits in common wheat. Genome 2020, 63, 27 -36.

AMA Style

Ting Li, Jian Ma, Yaya Zou, Guangdeng Chen, Pu-Yang Ding, Han Zhang, Cong-Cong Yang, Yang Mu, Huaping Tang, Yaxi Liu, Qiantao Jiang, Guo-Yue Chen, Peng-Fei Qi, Yu-Ming Wei, Youliang Zheng, Xiu-Jin Lan. Quantitative trait loci for seeding root traits and the relationships between root and agronomic traits in common wheat. Genome. 2020; 63 (1):27-36.

Chicago/Turabian Style

Ting Li; Jian Ma; Yaya Zou; Guangdeng Chen; Pu-Yang Ding; Han Zhang; Cong-Cong Yang; Yang Mu; Huaping Tang; Yaxi Liu; Qiantao Jiang; Guo-Yue Chen; Peng-Fei Qi; Yu-Ming Wei; Youliang Zheng; Xiu-Jin Lan. 2020. "Quantitative trait loci for seeding root traits and the relationships between root and agronomic traits in common wheat." Genome 63, no. 1: 27-36.

Journal article
Published: 29 October 2019 in Toxins
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Fusarium graminearum causes Fusarium head blight (FHB), a devastating disease of wheat. Salicylic acid (SA) is involved in the resistance of wheat to F. graminearum. Cell wall mannoprotein (CWM) is known to trigger defense responses in plants, but its role in the pathogenicity of F. graminearum remains unclear. Here, we characterized FgCWM1 (FG05_11315), encoding a CWM in F. graminearum. FgCWM1 was highly expressed in wheat spikes by 24 h after initial inoculation and was upregulated by SA. Disruption of FgCWM1 (ΔFgCWM1) reduced mannose and protein accumulation in the fungal cell wall, especially under SA treatment, and resulted in defective fungal cell walls, leading to increased fungal sensitivity to SA. The positive role of FgCWM1 in mannose and protein accumulation was confirmed by its expression in Saccharomyces cerevisiae. Compared with wild type (WT), ΔFgCWM1 exhibited reduced pathogenicity toward wheat, but it produced the same amount of deoxynivalenol both in culture and in spikes. Complementation of ΔFgCWM1 with FgCWM1 restored the WT phenotype. Localization analyses revealed that FgCWM1 was distributed on the cell wall, consistent with its structural role. Thus, FgCWM1 encodes a CWM protein that plays an important role in the cell wall integrity and pathogenicity of F. graminearum.

ACS Style

Ya-Zhou Zhang; Qing Chen; Cai-Hong Liu; Lu Lei; Yang Li; Kan Zhao; Mei-Qiao Wei; Zhen-Ru Guo; Yan Wang; Bin-Jie Xu; Yun-Feng Jiang; Li Kong; Yan-Lin Liu; Xiu-Jin Lan; Qian-Tao Jiang; Jian Ma; Ji-Rui Wang; Guo-Yue Chen; Yu-Ming Wei; You-Liang Zheng; Peng-Fei Qi. Fusarium graminearum FgCWM1 Encodes a Cell Wall Mannoprotein Conferring Sensitivity to Salicylic Acid and Virulence to Wheat. Toxins 2019, 11, 628 .

AMA Style

Ya-Zhou Zhang, Qing Chen, Cai-Hong Liu, Lu Lei, Yang Li, Kan Zhao, Mei-Qiao Wei, Zhen-Ru Guo, Yan Wang, Bin-Jie Xu, Yun-Feng Jiang, Li Kong, Yan-Lin Liu, Xiu-Jin Lan, Qian-Tao Jiang, Jian Ma, Ji-Rui Wang, Guo-Yue Chen, Yu-Ming Wei, You-Liang Zheng, Peng-Fei Qi. Fusarium graminearum FgCWM1 Encodes a Cell Wall Mannoprotein Conferring Sensitivity to Salicylic Acid and Virulence to Wheat. Toxins. 2019; 11 (11):628.

Chicago/Turabian Style

Ya-Zhou Zhang; Qing Chen; Cai-Hong Liu; Lu Lei; Yang Li; Kan Zhao; Mei-Qiao Wei; Zhen-Ru Guo; Yan Wang; Bin-Jie Xu; Yun-Feng Jiang; Li Kong; Yan-Lin Liu; Xiu-Jin Lan; Qian-Tao Jiang; Jian Ma; Ji-Rui Wang; Guo-Yue Chen; Yu-Ming Wei; You-Liang Zheng; Peng-Fei Qi. 2019. "Fusarium graminearum FgCWM1 Encodes a Cell Wall Mannoprotein Conferring Sensitivity to Salicylic Acid and Virulence to Wheat." Toxins 11, no. 11: 628.

Journal article
Published: 11 September 2019 in Agronomy
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Bioactive compounds such as phenols and phytic acid in wheat contribute to antioxidant capacities. (1) Background: Prior studies drew a general conclusion that the environment affected bioactive compounds greatly, but how the single environmental factor affects these characteristics remains unclear. (2) Methods: We conducted that twenty-eight winter wheat genotypes were grown in replicated trials at seven locations in China for two consecutive years and subdivided the environmental factor into five soil factors and six meteorological factors to evaluate the impact on the antioxidant capabilities and bioactive compounds contents of wheat grains by using principal component analysis (PCA). RT-PCR was used to identify gene expression of bioactive compounds under different conditions. (3) Results: Temperature affects bioactive compounds contents and antioxidant capacities greatly in wheat grains. Accumulation time, daylight length, and daily maximum temperature showed a high correlation with bioactive compounds contents and antioxidant capacities, especially in the vegetative growth phase. The gene TaMIPs related to phytic acid and TaPAL1, TaC3H1, TaC4H, Ta4CL1, and TaCOMT1 related to total phenolics had higher gene expression level with larger temperature differences in wheat grains. (4) Conclusions: The planting locations with higher temperatures and longer daylight length could produce higher contents of bioactive compounds and antioxidant capacities and the cooler temperatures of a planting location might produce wheat grains with lower phytic acid contents in wheat grains.

ACS Style

Zhien Pu; Liu; Zhengyang Li; Shihao Chen; Pengfei Qi; Yuming Wei; Youliang Zheng; Pu; Li; Chen; Qi; Wei; Qianqian Liu; Yongjian Liu. Planting Locations with Higher Temperature Produce More Bioactive Compounds and Antioxidant Capacities of Wheat. Agronomy 2019, 9, 538 .

AMA Style

Zhien Pu, Liu, Zhengyang Li, Shihao Chen, Pengfei Qi, Yuming Wei, Youliang Zheng, Pu, Li, Chen, Qi, Wei, Qianqian Liu, Yongjian Liu. Planting Locations with Higher Temperature Produce More Bioactive Compounds and Antioxidant Capacities of Wheat. Agronomy. 2019; 9 (9):538.

Chicago/Turabian Style

Zhien Pu; Liu; Zhengyang Li; Shihao Chen; Pengfei Qi; Yuming Wei; Youliang Zheng; Pu; Li; Chen; Qi; Wei; Qianqian Liu; Yongjian Liu. 2019. "Planting Locations with Higher Temperature Produce More Bioactive Compounds and Antioxidant Capacities of Wheat." Agronomy 9, no. 9: 538.

Regular article
Published: 05 June 2019 in New Phytologist
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De‐domestication is a unique evolutionary process during which crops re‐acquire wild‐like traits to survive and persist in agricultural fields without the need for human cultivation. The re‐acquisition of seed dispersal mechanisms is crucial for crop de‐domestication. Common wheat is an important cereal crop worldwide. Tibetan semi‐wild wheat is a potential de‐domesticated common wheat subspecies. However, the crucial genes responsible for its brittle rachis trait have not been identified. Genetic mapping, functional analyses, and phylogenetic analyses were completed to identify the gene associated with Qbr.sau‐5A, which is a major locus for the brittle rachis trait of Tibetan semi‐wild wheat. The cloned Qbr.sau‐5A gene is a new Q allele (Qt) with a 161‐bp transposon insertion in exon 5. Although Qt is expressed normally, its encoded peptide lacks some key features of the APETALA2 family. The abnormal functions of Qt in developing wheat spikes result in brittle rachises. Phylogenetic and genotyping analyses confirmed that Qt originated from Q in common wheat, and is naturally distributed only in Tibetan semi‐wild wheat populations. The identification of Qt provides new evidence regarding the origin of Tibetan semi‐wild wheat, and new insights into the re‐acquisition of wild traits during crop de‐domestication. This article is protected by copyright. All rights reserved.

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Yun‐Feng Jiang; Qing Chen; Yan Wang; Zhen‐Ru Guo; Bin‐Jie Xu; Jing Zhu; Ya‐Zhou Zhang; Xi Gong; Cui‐Hua Luo; Wang Wu; Cai‐Hong Liu; Li Kong; Mei Deng; Qian‐Tao Jiang; Xiu‐Jin Lan; Ji‐Rui Wang; Guo‐Yue Chen; You‐Liang Zheng; Yu‐Ming Wei; Peng‐Fei Qi. Re‐acquisition of the brittle rachis trait via a transposon insertion in domestication gene Q during wheat de‐domestication. New Phytologist 2019, 224, 961 -973.

AMA Style

Yun‐Feng Jiang, Qing Chen, Yan Wang, Zhen‐Ru Guo, Bin‐Jie Xu, Jing Zhu, Ya‐Zhou Zhang, Xi Gong, Cui‐Hua Luo, Wang Wu, Cai‐Hong Liu, Li Kong, Mei Deng, Qian‐Tao Jiang, Xiu‐Jin Lan, Ji‐Rui Wang, Guo‐Yue Chen, You‐Liang Zheng, Yu‐Ming Wei, Peng‐Fei Qi. Re‐acquisition of the brittle rachis trait via a transposon insertion in domestication gene Q during wheat de‐domestication. New Phytologist. 2019; 224 (2):961-973.

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Yun‐Feng Jiang; Qing Chen; Yan Wang; Zhen‐Ru Guo; Bin‐Jie Xu; Jing Zhu; Ya‐Zhou Zhang; Xi Gong; Cui‐Hua Luo; Wang Wu; Cai‐Hong Liu; Li Kong; Mei Deng; Qian‐Tao Jiang; Xiu‐Jin Lan; Ji‐Rui Wang; Guo‐Yue Chen; You‐Liang Zheng; Yu‐Ming Wei; Peng‐Fei Qi. 2019. "Re‐acquisition of the brittle rachis trait via a transposon insertion in domestication gene Q during wheat de‐domestication." New Phytologist 224, no. 2: 961-973.

Journal article
Published: 20 May 2019 in BMC Genomics
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Phytohormones are key regulators of plant growth, development, and signalling networks involved in responses to diverse biotic and abiotic stresses. Transcriptional reference maps of hormone responses have been reported for several model plant species such as Arabidopsis thaliana, Oryza sativa, and Brachypodium distachyon. However, because of species differences and the complexity of the wheat genome, these transcriptome data are not appropriate reference material for wheat studies. We comprehensively analysed the transcriptomic responses in wheat spikes to seven phytohormones, including indole acetic acid (IAA), gibberellic acid (GA), abscisic acid (ABA), ethylene (ET), cytokinin (CK), salicylic acid (SA), and methyl jasmonic acid (MeJA). A total of 3386 genes were differentially expressed at 24 h after the hormone treatments. Furthermore, 22.7% of these genes exhibited overlapping transcriptional responses for at least two hormones, implying there is crosstalk among phytohormones. We subsequently identified genes with expression levels that were significantly and differentially induced by a specific phytohormone (i.e., hormone-specific responses). The data for these hormone-responsive genes were then compared with the transcriptome data for wheat spikes exposed to biotic (Fusarium head blight) and abiotic (water deficit) stresses. Our data were used to develop a transcriptional reference map of hormone responses in wheat spikes.

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Peng-Fei Qi; Yun-Feng Jiang; Zhen-Ru Guo; Qing Chen; Thérèse Ouellet; Lu-Juan Zong; Zhen-Zhen Wei; Yan Wang; Ya-Zhou Zhang; Bin-Jie Xu; Li Kong; Mei Deng; Ji-Rui Wang; Guo-Yue Chen; Qian-Tao Jiang; Xiu-Jin Lan; Wei Li; Yu-Ming Wei; You-Liang Zheng. Transcriptional reference map of hormone responses in wheat spikes. BMC Genomics 2019, 20, 390 .

AMA Style

Peng-Fei Qi, Yun-Feng Jiang, Zhen-Ru Guo, Qing Chen, Thérèse Ouellet, Lu-Juan Zong, Zhen-Zhen Wei, Yan Wang, Ya-Zhou Zhang, Bin-Jie Xu, Li Kong, Mei Deng, Ji-Rui Wang, Guo-Yue Chen, Qian-Tao Jiang, Xiu-Jin Lan, Wei Li, Yu-Ming Wei, You-Liang Zheng. Transcriptional reference map of hormone responses in wheat spikes. BMC Genomics. 2019; 20 (1):390.

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Peng-Fei Qi; Yun-Feng Jiang; Zhen-Ru Guo; Qing Chen; Thérèse Ouellet; Lu-Juan Zong; Zhen-Zhen Wei; Yan Wang; Ya-Zhou Zhang; Bin-Jie Xu; Li Kong; Mei Deng; Ji-Rui Wang; Guo-Yue Chen; Qian-Tao Jiang; Xiu-Jin Lan; Wei Li; Yu-Ming Wei; You-Liang Zheng. 2019. "Transcriptional reference map of hormone responses in wheat spikes." BMC Genomics 20, no. 1: 390.

Journal article
Published: 22 January 2019 in Toxins
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Salicylic acid (SA) is a key defense hormone associated with wheat resistance against Fusarium head blight, which is a severe disease mainly caused by Fusarium graminearum. Although F. graminearum can metabolize SA, it remains unclear how this metabolic activity affects the wheat–F. graminearum interaction. In this study, we identified a salicylate hydroxylase gene (FG05_08116; FgNahG) in F. graminearum. This gene encodes a protein that catalyzes the conversion of SA to catechol. Additionally, FgNahG was widely distributed within hyphae. Disrupting the FgNahG gene (ΔFgNahG) led to enhanced sensitivity to SA, increased accumulation of SA in wheat spikes during the early infection stage and inhibited development of head blight symptoms. However, FgNahG did not affect mycotoxin production. Re-introducing a functional FgNahG gene into the ΔFgNahG mutant recovered the wild-type phenotype. Moreover, the expression of FgNahG in transgenic Arabidopsis thaliana decreased the SA concentration and the resistance of leaves to F. graminearum. These results indicate that the endogenous SA in wheat influences the resistance against F. graminearum. Furthermore, the capacity to metabolize SA is an important factor affecting the ability of F. graminearum to infect wheat plants.

ACS Style

Peng-Fei Qi; Ya-Zhou Zhang; Cai-Hong Liu; Qing Chen; Zhen-Ru Guo; Yan Wang; Bin-Jie Xu; Yun-Feng Jiang; Ting Zheng; Xi Gong; Cui-Hua Luo; Wang Wu; Li Kong; Mei Deng; Jian Ma; Xiu-Jin Lan; Qian-Tao Jiang; Yu-Ming Wei; Ji-Rui Wang; You-Liang Zheng. Functional Analysis of FgNahG Clarifies the Contribution of Salicylic Acid to Wheat (Triticum aestivum) Resistance against Fusarium Head Blight. Toxins 2019, 11, 59 .

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Peng-Fei Qi, Ya-Zhou Zhang, Cai-Hong Liu, Qing Chen, Zhen-Ru Guo, Yan Wang, Bin-Jie Xu, Yun-Feng Jiang, Ting Zheng, Xi Gong, Cui-Hua Luo, Wang Wu, Li Kong, Mei Deng, Jian Ma, Xiu-Jin Lan, Qian-Tao Jiang, Yu-Ming Wei, Ji-Rui Wang, You-Liang Zheng. Functional Analysis of FgNahG Clarifies the Contribution of Salicylic Acid to Wheat (Triticum aestivum) Resistance against Fusarium Head Blight. Toxins. 2019; 11 (2):59.

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Peng-Fei Qi; Ya-Zhou Zhang; Cai-Hong Liu; Qing Chen; Zhen-Ru Guo; Yan Wang; Bin-Jie Xu; Yun-Feng Jiang; Ting Zheng; Xi Gong; Cui-Hua Luo; Wang Wu; Li Kong; Mei Deng; Jian Ma; Xiu-Jin Lan; Qian-Tao Jiang; Yu-Ming Wei; Ji-Rui Wang; You-Liang Zheng. 2019. "Functional Analysis of FgNahG Clarifies the Contribution of Salicylic Acid to Wheat (Triticum aestivum) Resistance against Fusarium Head Blight." Toxins 11, no. 2: 59.

Journal article
Published: 10 August 2018 in International Journal of Molecular Sciences
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ATP-binding cassette (ABC) transporters hydrolyze ATP to transport a wide range of substrates. Fusarium graminearum is a major causal agent of Fusarium head blight, which is a severe disease in wheat worldwide. FgABCC9 (FG05_07325) encodes an ABC-C (ABC transporter family C) transporter in F. graminearum, which was highly expressed during the infection in wheat and was up-regulated by the plant defense hormone salicylic acid (SA) and the fungicide tebuconazole. The predicted tertiary structure of the FgABCC9 protein was consistent with the schematic of the ABC exporter. Deletion of FgABCC9 resulted in decreased mycelial growth, increased sensitivity to SA and tebuconazole, reduced accumulation of deoxynivalenol (DON), and less pathogenicity towards wheat. Re-introduction of a functional FgABCC9 gene into ΔFgABCC9 recovered the phenotypes of the wild type strain. Transgenic expression of FgABCC9 in Arabidopsis thaliana increased the accumulation of SA in its leaves without activating SA signaling, which suggests that FgABCC9 functions as an SA exporter. Taken together, FgABCC9 encodes an ABC exporter, which is critical for fungal exportation of SA, response to tebuconazole, mycelial growth, and pathogenicity towards wheat.

ACS Style

Peng-Fei Qi; Ya-Zhou Zhang; Cai-Hong Liu; Jing Zhu; Qing Chen; Zhen-Ru Guo; Yan Wang; Bin-Jie Xu; Ting Zheng; Yun-Feng Jiang; Jiang-Ping Wang; Cai-Yi Zhou; Xiang Feng; Li Kong; Xiu-Jin Lan; Qian-Tao Jiang; Yu-Ming Wei; You-Liang Zheng. Fusarium graminearum ATP-Binding Cassette Transporter Gene FgABCC9 Is Required for Its Transportation of Salicylic Acid, Fungicide Resistance, Mycelial Growth and Pathogenicity towards Wheat. International Journal of Molecular Sciences 2018, 19, 2351 .

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Peng-Fei Qi, Ya-Zhou Zhang, Cai-Hong Liu, Jing Zhu, Qing Chen, Zhen-Ru Guo, Yan Wang, Bin-Jie Xu, Ting Zheng, Yun-Feng Jiang, Jiang-Ping Wang, Cai-Yi Zhou, Xiang Feng, Li Kong, Xiu-Jin Lan, Qian-Tao Jiang, Yu-Ming Wei, You-Liang Zheng. Fusarium graminearum ATP-Binding Cassette Transporter Gene FgABCC9 Is Required for Its Transportation of Salicylic Acid, Fungicide Resistance, Mycelial Growth and Pathogenicity towards Wheat. International Journal of Molecular Sciences. 2018; 19 (8):2351.

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Peng-Fei Qi; Ya-Zhou Zhang; Cai-Hong Liu; Jing Zhu; Qing Chen; Zhen-Ru Guo; Yan Wang; Bin-Jie Xu; Ting Zheng; Yun-Feng Jiang; Jiang-Ping Wang; Cai-Yi Zhou; Xiang Feng; Li Kong; Xiu-Jin Lan; Qian-Tao Jiang; Yu-Ming Wei; You-Liang Zheng. 2018. "Fusarium graminearum ATP-Binding Cassette Transporter Gene FgABCC9 Is Required for Its Transportation of Salicylic Acid, Fungicide Resistance, Mycelial Growth and Pathogenicity towards Wheat." International Journal of Molecular Sciences 19, no. 8: 2351.

Journal article
Published: 09 August 2018 in Scientific Reports
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Basis for the effects of nitrogen (N) on wheat grain storage proteins (GSPs) and on the establishment of processing quality are far from clear. The response of GSPs and processing quality parameters to four N levels of four common wheat cultivars were investigated at two sites over two growing seasons. Except gluten index (GI), processing quality parameters as well as GSPs quantities were remarkably improved by increasing N level. N level explained 4.2~59.2% and 10.4~80.0% variability in GSPs fractions and processing quality parameters, respectively. The amount of N remobilized from vegetative organs except spike was significantly increased when enhancing N application. GSPs fractions and processing quality parameters except GI were only highly and positively correlated with the amount of N remobilized from stem with sheath. N reassimilation in grain was remarkably strengthened by the elevated activity and expression level of glutamine synthetase. Transcriptome analysis showed the molecular mechanism of seeds in response to N levels during 10~35 days post anthesis. Collectively, we provided comprehensive understanding of N-responding mechanisms with respect to wheat processing quality from N source to GSPs biosynthesis at the agronomic, physiological and molecular levels, and screened candidate genes for quality breeding.

ACS Style

Ting Zheng; Peng-Fei Qi; Yong-Li Cao; Ya-Nan Han; Hong-Liang Ma; Zhen-Ru Guo; Yan Wang; Yuan-Yuan Qiao; Shi-Yu Hua; Hai-Yue Yu; Jiang-Ping Wang; Jing Zhu; Cai-Yi Zhou; Ya-Zhou Zhang; Qing Chen; Li Kong; Ji-Rui Wang; Qian-Tao Jiang; Ze-Hong Yan; Xiu-Jin Lan; Gao-Qiong Fan; Yu-Ming Wei; You-Liang Zheng. Mechanisms of wheat (Triticum aestivum) grain storage proteins in response to nitrogen application and its impacts on processing quality. Scientific Reports 2018, 8, 11928 .

AMA Style

Ting Zheng, Peng-Fei Qi, Yong-Li Cao, Ya-Nan Han, Hong-Liang Ma, Zhen-Ru Guo, Yan Wang, Yuan-Yuan Qiao, Shi-Yu Hua, Hai-Yue Yu, Jiang-Ping Wang, Jing Zhu, Cai-Yi Zhou, Ya-Zhou Zhang, Qing Chen, Li Kong, Ji-Rui Wang, Qian-Tao Jiang, Ze-Hong Yan, Xiu-Jin Lan, Gao-Qiong Fan, Yu-Ming Wei, You-Liang Zheng. Mechanisms of wheat (Triticum aestivum) grain storage proteins in response to nitrogen application and its impacts on processing quality. Scientific Reports. 2018; 8 (1):11928.

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Ting Zheng; Peng-Fei Qi; Yong-Li Cao; Ya-Nan Han; Hong-Liang Ma; Zhen-Ru Guo; Yan Wang; Yuan-Yuan Qiao; Shi-Yu Hua; Hai-Yue Yu; Jiang-Ping Wang; Jing Zhu; Cai-Yi Zhou; Ya-Zhou Zhang; Qing Chen; Li Kong; Ji-Rui Wang; Qian-Tao Jiang; Ze-Hong Yan; Xiu-Jin Lan; Gao-Qiong Fan; Yu-Ming Wei; You-Liang Zheng. 2018. "Mechanisms of wheat (Triticum aestivum) grain storage proteins in response to nitrogen application and its impacts on processing quality." Scientific Reports 8, no. 1: 11928.

Journal article
Published: 21 March 2018 in International Journal of Molecular Sciences
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Glu-1Ay, one of six genes encoding a high molecular weight glutenin subunit (HMW-GS), is frequently silenced in hexaploid common wheat. Here, an active allele of Glu-1Ay was integrated from wild emmer wheat (Triticum turgidum ssp. dicoccoides) accession D97 into the common wheat (Triticum aestivum) cultivar Chuannong 16 via the repeated self-fertilization of the pentaploid interspecific hybrid, culminating in the selection of a line TaAy7-40 shown to express the wild emmer Glu-1Ay allele. The open reading frame of this allele was a 1830 bp long sequence, demonstrated by its heterologous expression in Escherichia coli to encode a 608-residue polypeptide. Its nucleotide sequence was 99.2% identical to that of the sequence within the wild emmer parent. The TaAy7-40 introgression line containing the active Glu-1Ay allele showed higher protein content, higher sodium dodecyl sulfate (SDS) sedimentation value, higher content of wet gluten in the flour, higher grain weight, and bigger grain size than Chuannong 16. The end-use quality parameters of the TaAy7-40 were superior to those of the medium gluten common wheat cultivars Mianmai 37 and Neimai 9. Thus, the active Glu-1Ay allele might be of potential value in breeding programs designed to improve wheat flour quality.

ACS Style

Zhenzhen Wang; Lin Huang; Bihua Wu; Jiliang Hu; Zilong Jiang; Pengfei Qi; Youliang Zheng; Dengcai Liu. Characterization of an Integrated Active Glu-1Ay Allele in Common Wheat from Wild Emmer and Its Potential Role in Flour Improvement. International Journal of Molecular Sciences 2018, 19, 923 .

AMA Style

Zhenzhen Wang, Lin Huang, Bihua Wu, Jiliang Hu, Zilong Jiang, Pengfei Qi, Youliang Zheng, Dengcai Liu. Characterization of an Integrated Active Glu-1Ay Allele in Common Wheat from Wild Emmer and Its Potential Role in Flour Improvement. International Journal of Molecular Sciences. 2018; 19 (4):923.

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Zhenzhen Wang; Lin Huang; Bihua Wu; Jiliang Hu; Zilong Jiang; Pengfei Qi; Youliang Zheng; Dengcai Liu. 2018. "Characterization of an Integrated Active Glu-1Ay Allele in Common Wheat from Wild Emmer and Its Potential Role in Flour Improvement." International Journal of Molecular Sciences 19, no. 4: 923.

Journal article
Published: 01 March 2018 in G3 Genes|Genomes|Genetics
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Spike density and processing quality are important traits in modern wheat production and are controlled by multiple gene loci. The associated genes have been intensively studied and new discoveries have been constantly reported during the past few decades. However, no gene playing a significant role in the development of these two traits has been identified. In the current study, a common wheat mutant with extremely compact spikes and good processing quality was isolated and characterized. A new allele (Qc1) of the Q gene (an important domestication gene) responsible for the mutant phenotype was cloned, and the molecular mechanism for the mutant phenotype was studied. Results revealed that Qc1 originated from a point mutation that interferes with the miRNA172-directed cleavage of Q transcripts, leading to its overexpression. It also reduces the longitudinal cell size of rachises, resulting in an increased spike density. Furthermore, Qc1 increases the number of vascular bundles, which suggests a higher efficiency in the transportation of assimilates in the spikes of the mutant than that of wild type. This accounts for the improved processing quality. The effects of Qc1 on spike density and wheat processing quality were confirmed by analyzing nine common wheat mutants possessing four different Qc alleles. These results deepen our understanding of the key roles of Q gene, and provide new insights for the potential application of Qc alleles in wheat quality breeding.

ACS Style

Bin-Jie Xu; Qing Chen; Ting Zheng; Yun-Feng Jiang; Yuan-Yuan Qiao; Zhen-Ru Guo; Yong-Li Cao; Yan Wang; Ya-Zhou Zhang; Lu-Juan Zong; Jing Zhu; Cai-Hong Liu; Qian-Tao Jiang; Xiu-Jin Lan; Jian Ma; Ji-Rui Wang; You-Liang Zheng; Yu-Ming Wei; Peng-Fei Qi. An Overexpressed Q Allele Leads to Increased Spike Density and Improved Processing Quality in Common Wheat (Triticum aestivum). G3 Genes|Genomes|Genetics 2018, 8, 771 -778.

AMA Style

Bin-Jie Xu, Qing Chen, Ting Zheng, Yun-Feng Jiang, Yuan-Yuan Qiao, Zhen-Ru Guo, Yong-Li Cao, Yan Wang, Ya-Zhou Zhang, Lu-Juan Zong, Jing Zhu, Cai-Hong Liu, Qian-Tao Jiang, Xiu-Jin Lan, Jian Ma, Ji-Rui Wang, You-Liang Zheng, Yu-Ming Wei, Peng-Fei Qi. An Overexpressed Q Allele Leads to Increased Spike Density and Improved Processing Quality in Common Wheat (Triticum aestivum). G3 Genes|Genomes|Genetics. 2018; 8 (3):771-778.

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Bin-Jie Xu; Qing Chen; Ting Zheng; Yun-Feng Jiang; Yuan-Yuan Qiao; Zhen-Ru Guo; Yong-Li Cao; Yan Wang; Ya-Zhou Zhang; Lu-Juan Zong; Jing Zhu; Cai-Hong Liu; Qian-Tao Jiang; Xiu-Jin Lan; Jian Ma; Ji-Rui Wang; You-Liang Zheng; Yu-Ming Wei; Peng-Fei Qi. 2018. "An Overexpressed Q Allele Leads to Increased Spike Density and Improved Processing Quality in Common Wheat (Triticum aestivum)." G3 Genes|Genomes|Genetics 8, no. 3: 771-778.

Journal article
Published: 01 March 2018 in Genome
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We evaluated the SGP-1 protein composition of 368 Chinese wheat landraces using SDS-PAGE. The SGP-D1 null type was identified in three accessions (Xiaoqingmang, Pushanbamai, and P119). An 18-bp deletion and 9-bp variation were found at the junction region of the 7th intron and 8th exon, leading to deletion of the intron–exon junction recognition site AG when aligned the 8261-bp DNA sequence of TaSSIIa-D in Pushanbamai with that of Chinese Spring. Four cDNA types with mis-spliced isoforms were subsequently detected through amplification of TaSSIIa-D cDNAs. Among these, nine type II cDNAs with a 16-bp deletion in the 8th exon were detected, indicating that the major transcriptional pattern of TaSSIIa in Pushanbamai is type II. In the type IV cDNA, a 97-bp sequence remains undeleted in the end of the 5th exon. The amylose content in Pushanbamai was significantly higher than that in all control lines under field conditions, which suggested that deletion of SGP-D1 has an efficient impact on amylose content. As the TaSSIIa gene plays an important role in regulating the content of amylose, it is anticipated that these natural variants of TaSSIIa-D will provide useful resources for quality improvement in wheat.

ACS Style

Yan-Jie Zhou; Qiang Yang; Xiao-Juan Zhong; Hua-Ping Tang; Mei Deng; Jian Ma; Peng-Fei Qi; Ji-Rui Wang; Guo-Yue Chen; Ya-Xi Liu; Zhen-Xiang Lu; Wei Li; Xiu-Jin Lan; Yu-Ming Wei; You-Liang Zheng; Qian-Tao Jiang. Alternative splicing results in a lack of starch synthase IIa-D in Chinese wheat landrace. Genome 2018, 61, 201 -208.

AMA Style

Yan-Jie Zhou, Qiang Yang, Xiao-Juan Zhong, Hua-Ping Tang, Mei Deng, Jian Ma, Peng-Fei Qi, Ji-Rui Wang, Guo-Yue Chen, Ya-Xi Liu, Zhen-Xiang Lu, Wei Li, Xiu-Jin Lan, Yu-Ming Wei, You-Liang Zheng, Qian-Tao Jiang. Alternative splicing results in a lack of starch synthase IIa-D in Chinese wheat landrace. Genome. 2018; 61 (3):201-208.

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Yan-Jie Zhou; Qiang Yang; Xiao-Juan Zhong; Hua-Ping Tang; Mei Deng; Jian Ma; Peng-Fei Qi; Ji-Rui Wang; Guo-Yue Chen; Ya-Xi Liu; Zhen-Xiang Lu; Wei Li; Xiu-Jin Lan; Yu-Ming Wei; You-Liang Zheng; Qian-Tao Jiang. 2018. "Alternative splicing results in a lack of starch synthase IIa-D in Chinese wheat landrace." Genome 61, no. 3: 201-208.

Journal article
Published: 20 December 2017 in Biologia
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ADP-glucose pyrophosphorylase (AGP) consists of two large (AGP-L) and two small (AGP-S) subunits; it regulates the limiting step in the biosynthesis of starch. Here, we isolated the full-length cDNA sequence of the AGP-S gene (designed as

ACS Style

Xiao-Wei Zhang; Qing Wang; Ling-Ling Zhang; Xiao-Juan Zhong; Qian-Tao Jiang; Jian Ma; Peng-Fei Qi; Wei Li; Guo-Yue Chen; Xiu-Jin Lan; Mei Deng; Zhen-Xiang Lu; Zong-Jun Pu; Yu-Ming Wei; You-Liang Zheng. Cloning and characterization of Agp1, the gene encoding the small subunit of ADP-glucose pyrophosphorylase from wheat and its relatives. Biologia 2017, 72, 1446 -1453.

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Xiao-Wei Zhang, Qing Wang, Ling-Ling Zhang, Xiao-Juan Zhong, Qian-Tao Jiang, Jian Ma, Peng-Fei Qi, Wei Li, Guo-Yue Chen, Xiu-Jin Lan, Mei Deng, Zhen-Xiang Lu, Zong-Jun Pu, Yu-Ming Wei, You-Liang Zheng. Cloning and characterization of Agp1, the gene encoding the small subunit of ADP-glucose pyrophosphorylase from wheat and its relatives. Biologia. 2017; 72 (12):1446-1453.

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Xiao-Wei Zhang; Qing Wang; Ling-Ling Zhang; Xiao-Juan Zhong; Qian-Tao Jiang; Jian Ma; Peng-Fei Qi; Wei Li; Guo-Yue Chen; Xiu-Jin Lan; Mei Deng; Zhen-Xiang Lu; Zong-Jun Pu; Yu-Ming Wei; You-Liang Zheng. 2017. "Cloning and characterization of Agp1, the gene encoding the small subunit of ADP-glucose pyrophosphorylase from wheat and its relatives." Biologia 72, no. 12: 1446-1453.

Journal article
Published: 07 April 2017 in Scientific Reports
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Fusarium graminearum is the major causal agent of fusarium head blight in wheat, a serious disease worldwide. Linoleic acid isomerase (LAI) catalyses the transformation of linoleic acid (LA) to conjugated linoleic acid (CLA), which is beneficial for human health. We characterised a cis-12 LAI gene of F. graminearum (FGSG_02668; FgLAI12), which was downregulated by salicylic acid (SA), a plant defence hormone. Disruption of FgLAI12 in F. graminearum resulted in decreased accumulation of cis-9,trans-11 CLA, enhanced sensitivity to SA, and increased accumulation of LA and SA in wheat spikes during infection. In addition, mycelial growth, accumulation of deoxynivalenol, and pathogenicity in wheat spikes were reduced. Re-introduction of a functional FgLAI12 gene into ΔFgLAI12 recovered the wild-type phenotype. Fluorescent microscopic analysis showed that FgLAI12 protein was usually expressed in the septa zone of conidia and the vacuole of hyphae, but was expressed in the cell membrane of hyphae in response to exogenous LA, which may be an element of LA metabolism during infection by F. graminearum. The cis-12 LAI enzyme encoded by FgLAI12 is critical for fungal response to SA, mycelial growth and virulence in wheat. The gene FgLAI12 is potentially valuable for biotechnological synthesis of cis-9,trans-11 CLA.

ACS Style

Ya-Zhou Zhang; Zhen-Zhen Wei; Cai-Hong Liu; Qing Chen; Bin-Jie Xu; Zhen-Ru Guo; Yong-Li Cao; Yan Wang; Ya-Nan Han; Chen Chen; Xiang Feng; Yuan-Yuan Qiao; Lu-Juan Zong; Ting Zheng; Mei Deng; Qian-Tao Jiang; Wei Li; You-Liang Zheng; Yu-Ming Wei; Peng-Fei Qi. Linoleic acid isomerase gene FgLAI12 affects sensitivity to salicylic acid, mycelial growth and virulence of Fusarium graminearum. Scientific Reports 2017, 7, 46129 .

AMA Style

Ya-Zhou Zhang, Zhen-Zhen Wei, Cai-Hong Liu, Qing Chen, Bin-Jie Xu, Zhen-Ru Guo, Yong-Li Cao, Yan Wang, Ya-Nan Han, Chen Chen, Xiang Feng, Yuan-Yuan Qiao, Lu-Juan Zong, Ting Zheng, Mei Deng, Qian-Tao Jiang, Wei Li, You-Liang Zheng, Yu-Ming Wei, Peng-Fei Qi. Linoleic acid isomerase gene FgLAI12 affects sensitivity to salicylic acid, mycelial growth and virulence of Fusarium graminearum. Scientific Reports. 2017; 7 (1):46129.

Chicago/Turabian Style

Ya-Zhou Zhang; Zhen-Zhen Wei; Cai-Hong Liu; Qing Chen; Bin-Jie Xu; Zhen-Ru Guo; Yong-Li Cao; Yan Wang; Ya-Nan Han; Chen Chen; Xiang Feng; Yuan-Yuan Qiao; Lu-Juan Zong; Ting Zheng; Mei Deng; Qian-Tao Jiang; Wei Li; You-Liang Zheng; Yu-Ming Wei; Peng-Fei Qi. 2017. "Linoleic acid isomerase gene FgLAI12 affects sensitivity to salicylic acid, mycelial growth and virulence of Fusarium graminearum." Scientific Reports 7, no. 1: 46129.