This page has only limited features, please log in for full access.
APETALA2-like genes encode plant-specific transcription factors, some of which possess one microRNA172 (miR172) binding site. The miR172 and its target euAP2 genes are involved in the process of phase transformation and flower organ development in many plants. However, the roles of miR172 and its target AP2 genes remain largely unknown in Brassica napus (B. napus). In this study, 19 euAP2 and four miR172 genes were identified in the B. napus genome. A sequence analysis suggested that 17 euAP2 genes were targeted by Bna-miR172 in the 3′ coding region. EuAP2s were classified into five major groups in B.napus. This classification was consistent with the exon-intron structure and motif organization. An analysis of the nonsynonymous and synonymous substitution rates revealed that the euAP2 genes had gone through purifying selection. Whole genome duplication (WGD) or segmental duplication events played a major role in the expansion of the euAP2 gene family. A cis-regulatory element (CRE) analysis suggested that the euAP2s were involved in the response to light, hormones, stress, and developmental processes including circadian control, endosperm and meristem expression. Expression analysis of the miR172-targeted euAP2s in nine different tissues showed diverse spatiotemporal expression patterns. Most euAP2 genes were highly expressed in the floral organs, suggesting their specific functions in flower development. BnaAP2–1, BnaAP2–5 and BnaTOE1–2 had higher expression levels in late-flowering material than early-flowering material based on RNA-seq and qRT-PCR, indicating that they may act as floral suppressors. Overall, analyses of the evolution, structure, tissue specificity and expression of the euAP2 genes were peformed in B.napus. Based on the RNA-seq and experimental data, euAP2 may be involved in flower development. Three euAP2 genes (BnaAP2–1, BnaAP2–5 and BnaTOE1–2) might be regarded as floral suppressors. The results of this study provide insights for further functional characterization of the miR172 /euAP2 module in B.napus.
Tengyue Wang; Xiaoke Ping; Yanru Cao; Hongju Jian; Yumin Gao; Jia Wang; Yingchao Tan; Xinfu Xu; Kun Lu; Jiana Li; Liezhao Liu. Genome-wide exploration and characterization of miR172/euAP2 genes in Brassica napus L. for likely role in flower organ development. BMC Plant Biology 2019, 19, 1 -15.
AMA StyleTengyue Wang, Xiaoke Ping, Yanru Cao, Hongju Jian, Yumin Gao, Jia Wang, Yingchao Tan, Xinfu Xu, Kun Lu, Jiana Li, Liezhao Liu. Genome-wide exploration and characterization of miR172/euAP2 genes in Brassica napus L. for likely role in flower organ development. BMC Plant Biology. 2019; 19 (1):1-15.
Chicago/Turabian StyleTengyue Wang; Xiaoke Ping; Yanru Cao; Hongju Jian; Yumin Gao; Jia Wang; Yingchao Tan; Xinfu Xu; Kun Lu; Jiana Li; Liezhao Liu. 2019. "Genome-wide exploration and characterization of miR172/euAP2 genes in Brassica napus L. for likely role in flower organ development." BMC Plant Biology 19, no. 1: 1-15.
Lignin is an important biological polymer in plants that is necessary for plant secondary cell wall ontogenesis. The laccase (LAC) gene family catalyzes lignification and has been suggested to play a vital role in the plant kingdom. In this study, we identified 45 LAC genes from the Brassica napus genome (BnLACs), 25 LAC genes from the Brassica rapa genome (BrLACs) and 8 LAC genes from the Brassica oleracea genome (BoLACs). These LAC genes could be divided into five groups in a cladogram and members in same group had similar structures and conserved motifs. All BnLACs contained hormone- and stress- related elements determined by cis-element analysis. The expression of BnLACs was relatively higher in the root, seed coat and stem than in other tissues. Furthermore, BnLAC4 and its predicted downstream genes showed earlier expression in the silique pericarps of short silique lines than long silique lines. Three miRNAs (miR397a, miR397b and miR6034) target 11 BnLACs were also predicted. The expression changes of BnLACs under series of stresses were further investigated by RNA sequencing (RNA-seq) and quantitative real-time polymerase chain reaction (qRT-PCR). The study will give a deeper understanding of the LAC gene family evolution and functions in B. napus.
Xiaoke Ping; Tengyue Wang; Na Lin; Feifei Di; Yangyang Li; Hongju Jian; Hao Wang; Kun Lu; Jiana Li; Xinfu Xu; Liezhao Liu. Genome-Wide Identification of the LAC Gene Family and Its Expression Analysis Under Stress in Brassica napus. Molecules 2019, 24, 1985 .
AMA StyleXiaoke Ping, Tengyue Wang, Na Lin, Feifei Di, Yangyang Li, Hongju Jian, Hao Wang, Kun Lu, Jiana Li, Xinfu Xu, Liezhao Liu. Genome-Wide Identification of the LAC Gene Family and Its Expression Analysis Under Stress in Brassica napus. Molecules. 2019; 24 (10):1985.
Chicago/Turabian StyleXiaoke Ping; Tengyue Wang; Na Lin; Feifei Di; Yangyang Li; Hongju Jian; Hao Wang; Kun Lu; Jiana Li; Xinfu Xu; Liezhao Liu. 2019. "Genome-Wide Identification of the LAC Gene Family and Its Expression Analysis Under Stress in Brassica napus." Molecules 24, no. 10: 1985.
Optimum flowering time is a key agronomic trait in Brassica napus. To investigate the genetic architecture and genetic regulation of flowering time in this important crop, we conducted quantitative trait loci (QTL) analysis of flowering time in a recombinant inbred line (RIL) population, including lines with extreme differences in flowering time, in six environments, along with RNA-Seq analysis. We detected 27 QTLs distributed on eight chromosomes among six environments, including one major QTL on chromosome C02 that explained 11–25% of the phenotypic variation and was stably detected in all six environments. RNA-Seq analysis revealed 105 flowering time-related differentially expressed genes (DEGs) that play roles in the circadian clock/photoperiod, autonomous pathway, and hormone and vernalization pathways. We focused on DEGs related to the regulation of flowering time, especially DEGs in QTL regions. We identified 45 flowering time-related genes in these QTL regions, eight of which are DEGs, including key flowering time genes PSEUDO RESPONSE REGULATOR 7 (PRR7) and FY (located in a major QTL region on C02). These findings provide insights into the genetic architecture of flowering time in B. napus. The online version of this article (10.1186/s12864-018-5356-8) contains supplementary material, which is available to authorized users.
Hongju Jian; Aoxiang Zhang; Jinqi Ma; Tengyue Wang; Bo Yang; Lan Shuan Shuang; Min Liu; Jiana Li; Xinfu Xu; Andrew H. Paterson; Liezhao Liu. Joint QTL mapping and transcriptome sequencing analysis reveal candidate flowering time genes in Brassica napus L. BMC Genomics 2019, 20, 21 .
AMA StyleHongju Jian, Aoxiang Zhang, Jinqi Ma, Tengyue Wang, Bo Yang, Lan Shuan Shuang, Min Liu, Jiana Li, Xinfu Xu, Andrew H. Paterson, Liezhao Liu. Joint QTL mapping and transcriptome sequencing analysis reveal candidate flowering time genes in Brassica napus L. BMC Genomics. 2019; 20 (1):21.
Chicago/Turabian StyleHongju Jian; Aoxiang Zhang; Jinqi Ma; Tengyue Wang; Bo Yang; Lan Shuan Shuang; Min Liu; Jiana Li; Xinfu Xu; Andrew H. Paterson; Liezhao Liu. 2019. "Joint QTL mapping and transcriptome sequencing analysis reveal candidate flowering time genes in Brassica napus L." BMC Genomics 20, no. 1: 21.
NODULE-INCEPTION-like proteins (NLPs) are conserved, plant-specific transcription factors that play crucial roles in responses to nitrogen deficiency. However, the evolutionary relationships and characteristics of NLP family genes in Brassica napus are unclear. In this study, we identified 31 NLP genes in B. napus, including 16 genes located in the A subgenome and 15 in the C subgenome. Subcellular localization predictions indicated that most BnaNLP proteins are localized to the nucleus. Phylogenetic analysis suggested that the NLP gene family could be divided into three groups and that at least three ancient copies of NLP genes existed in the ancestor of both monocots and dicots prior to their divergence. The ancestor of group III NLP genes may have experienced duplication more than once in the Brassicaceae species. Three-dimensional structural analysis suggested that 14 amino acids in BnaNLP7-1 protein are involved in DNA binding, whereas no binding sites were identified in the two RWP-RK and PB1 domains conserved in BnaNLP proteins. Expression profile analysis indicated that BnaNLP genes are expressed in most organs but tend to be highly expressed in a single organ. For example, BnaNLP6 subfamily members are primarily expressed in roots, while the four BnaNLP7 subfamily members are highly expressed in leaves. BnaNLP genes also showed different expression patterns in response to nitrogen-deficient conditions. Under nitrogen deficiency, all members of the BnaNLP1/4/5/9 subfamilies were upregulated, all BnaNLP2/6 subfamily members were downregulated, and BnaNLP7/8 subfamily members showed various expression patterns in different organs. These results provide a comprehensive evolutionary history of NLP genes in B. napus, and insight into the biological functions of BnaNLP genes in response to nitrogen deficiency.
Miao Liu; Wei Chang; Yonghai Fan; Wei Sun; Cunmin Qu; Kai Zhang; Liezhao Liu; Xingfu Xu; Zhanglin Tang; Jiana Li; Kun Lu. Genome-Wide Identification and Characterization of NODULE-INCEPTION-Like Protein (NLP) Family Genes in Brassica napus. International Journal of Molecular Sciences 2018, 19, 2270 .
AMA StyleMiao Liu, Wei Chang, Yonghai Fan, Wei Sun, Cunmin Qu, Kai Zhang, Liezhao Liu, Xingfu Xu, Zhanglin Tang, Jiana Li, Kun Lu. Genome-Wide Identification and Characterization of NODULE-INCEPTION-Like Protein (NLP) Family Genes in Brassica napus. International Journal of Molecular Sciences. 2018; 19 (8):2270.
Chicago/Turabian StyleMiao Liu; Wei Chang; Yonghai Fan; Wei Sun; Cunmin Qu; Kai Zhang; Liezhao Liu; Xingfu Xu; Zhanglin Tang; Jiana Li; Kun Lu. 2018. "Genome-Wide Identification and Characterization of NODULE-INCEPTION-Like Protein (NLP) Family Genes in Brassica napus." International Journal of Molecular Sciences 19, no. 8: 2270.
MicroRNAs (miRNAs) have important roles in regulating stress-response genes in plants. However, identification of miRNAs and the corresponding target genes that are induced in response to cadmium (Cd) stress in Brassica napus remains limited. In the current study, we sequenced three small-RNA libraries from B. napus after 0 days, 1 days, and 3 days of Cd treatment. In total, 44 known miRNAs (belonging to 27 families) and 103 novel miRNAs were identified. A comprehensive analysis of miRNA expression profiles found 39 differentially expressed miRNAs between control and Cd-treated plants; 13 differentially expressed miRNAs were confirmed by qRT-PCR. Characterization of the corresponding target genes indicated functions in processes including transcription factor regulation, biotic stress response, ion homeostasis, and secondary metabolism. Furthermore, we propose a hypothetical model of the Cd-response mechanism in B. napus. Combined with qRT-PCR confirmation, our data suggested that miRNAs were involved in the regulations of TFs, biotic stress defense, ion homeostasis and secondary metabolism synthesis to respond Cd stress in B. napus.
Hongju Jian; Bo Yang; Aoxiang Zhang; Jinqi Ma; Yiran Ding; Zhiyou Chen; Jiana Li; Xinfu Xu; Liezhao Liu. Genome-Wide Identification of MicroRNAs in Response to Cadmium Stress in Oilseed Rape (Brassica napus L.) Using High-Throughput Sequencing. International Journal of Molecular Sciences 2018, 19, 1431 .
AMA StyleHongju Jian, Bo Yang, Aoxiang Zhang, Jinqi Ma, Yiran Ding, Zhiyou Chen, Jiana Li, Xinfu Xu, Liezhao Liu. Genome-Wide Identification of MicroRNAs in Response to Cadmium Stress in Oilseed Rape (Brassica napus L.) Using High-Throughput Sequencing. International Journal of Molecular Sciences. 2018; 19 (5):1431.
Chicago/Turabian StyleHongju Jian; Bo Yang; Aoxiang Zhang; Jinqi Ma; Yiran Ding; Zhiyou Chen; Jiana Li; Xinfu Xu; Liezhao Liu. 2018. "Genome-Wide Identification of MicroRNAs in Response to Cadmium Stress in Oilseed Rape (Brassica napus L.) Using High-Throughput Sequencing." International Journal of Molecular Sciences 19, no. 5: 1431.
The basic region/leucine zipper motif (bZIP) transcription factor family is one of the largest families of transcriptional regulators in plants. bZIP genes have been systematically characterized in some plants, but not in rapeseed (Brassica napus). In this study, we identified 247 BnbZIP genes in the rapeseed genome, which we classified into 10 subfamilies based on phylogenetic analysis of their deduced protein sequences. The BnbZIP genes were grouped into functional clades with Arabidopsis genes with similar putative functions, indicating functional conservation. Genome mapping analysis revealed that the BnbZIPs are distributed unevenly across all 19 chromosomes, and that some of these genes arose through whole-genome duplication and dispersed duplication events. All expression profiles of 247 bZIP genes were extracted from RNA-sequencing data obtained from 17 different B. napus ZS11 tissues with 42 various developmental stages. These genes exhibited different expression patterns in various tissues, revealing that these genes are differentially regulated. Our results provide a valuable foundation for functional dissection of the different BnbZIP homologs in B. napus and its parental lines and for molecular breeding studies of bZIP genes in B. napus.
Yan Zhou; Daixiang Xu; Ledong Jia; Xiaohu Huang; Guoqiang Ma; Shuxian Wang; Meichen Zhu; Aoxiang Zhang; Mingwei Guan; Kun Lu; Xinfu Xu; Rui Wang; Jiana Li; Cunmin Qu. Genome-Wide Identification and Structural Analysis of bZIP Transcription Factor Genes in Brassica napus. Genes 2017, 8, 288 .
AMA StyleYan Zhou, Daixiang Xu, Ledong Jia, Xiaohu Huang, Guoqiang Ma, Shuxian Wang, Meichen Zhu, Aoxiang Zhang, Mingwei Guan, Kun Lu, Xinfu Xu, Rui Wang, Jiana Li, Cunmin Qu. Genome-Wide Identification and Structural Analysis of bZIP Transcription Factor Genes in Brassica napus. Genes. 2017; 8 (10):288.
Chicago/Turabian StyleYan Zhou; Daixiang Xu; Ledong Jia; Xiaohu Huang; Guoqiang Ma; Shuxian Wang; Meichen Zhu; Aoxiang Zhang; Mingwei Guan; Kun Lu; Xinfu Xu; Rui Wang; Jiana Li; Cunmin Qu. 2017. "Genome-Wide Identification and Structural Analysis of bZIP Transcription Factor Genes in Brassica napus." Genes 8, no. 10: 288.
Candidate genes associated with lignin and lodging traits were identified by combining phenotypic, genotypic, and gene expression data in B. napus. Brassica napus is one of the world’s most important oilseed crops, but its yield can be dramatically reduced by lodging, bending, and falling of its vertical stems. Lignin has been shown to contribute to stem mechanical strength. In this study, we found that the syringyl/guaiacyl (S/G) monolignol ratio exhibits a significant negative correlation with disease and lodging resistance. A total of 92 and 50 SNP and SSR loci, respectively, were found to be significantly associated with five traits, breaking force, breaking strength, lodging coefficient, acid detergent lignin content, and the S/G monolignol ratio using GWAS. To identify novel genes involved in lignin biosynthesis, transcriptome sequencing of high- (H) and low (L)-ADL content accessions was performed. The up-regulated genes were mainly involved in glycoside catabolic processes (especially glucosinolate catabolism) and cell wall biogenesis, while down-regulated genes were involved in glucosinolate biosynthesis, indicating that crosstalk exists between glucosinolate metabolic processes and lignin biosynthesis. Integrating this differential expression with the GWAS analysis, we identified four candidate genes regulating lignin, including glycosyl hydrolase (BnaA01g00480D), CYT1 (BnaA04g22820D), and two encoding transcription factors, SHINE1 (ERF family) and DAR6 (LIM family). This study provides insight into the genetic control of lodging and lignin in B. napus.
Lijuan Wei; Hongju Jian; Kun Lu; Nengwen Yin; Jia Wang; Xiujian Duan; Wei Li; Liezhao Liu; Xinfu Xu; Rui Wang; Andrew H. Paterson; Jiana Li. Genetic and transcriptomic analyses of lignin- and lodging-related traits in Brassica napus. Theoretical and Applied Genetics 2017, 130, 1961 -1973.
AMA StyleLijuan Wei, Hongju Jian, Kun Lu, Nengwen Yin, Jia Wang, Xiujian Duan, Wei Li, Liezhao Liu, Xinfu Xu, Rui Wang, Andrew H. Paterson, Jiana Li. Genetic and transcriptomic analyses of lignin- and lodging-related traits in Brassica napus. Theoretical and Applied Genetics. 2017; 130 (9):1961-1973.
Chicago/Turabian StyleLijuan Wei; Hongju Jian; Kun Lu; Nengwen Yin; Jia Wang; Xiujian Duan; Wei Li; Liezhao Liu; Xinfu Xu; Rui Wang; Andrew H. Paterson; Jiana Li. 2017. "Genetic and transcriptomic analyses of lignin- and lodging-related traits in Brassica napus." Theoretical and Applied Genetics 130, no. 9: 1961-1973.
Min Tan; Fang Liao; Lintao Hou; Jia Wang; Lijuan Wei; Hongju Jian; Xinfu Xu; Jiana Li; Liezhao Liu. Genome-wide association analysis of seed germination percentage and germination index in Brassica napus L. under salt and drought stresses. Euphytica 2017, 213, 1 .
AMA StyleMin Tan, Fang Liao, Lintao Hou, Jia Wang, Lijuan Wei, Hongju Jian, Xinfu Xu, Jiana Li, Liezhao Liu. Genome-wide association analysis of seed germination percentage and germination index in Brassica napus L. under salt and drought stresses. Euphytica. 2017; 213 (2):1.
Chicago/Turabian StyleMin Tan; Fang Liao; Lintao Hou; Jia Wang; Lijuan Wei; Hongju Jian; Xinfu Xu; Jiana Li; Liezhao Liu. 2017. "Genome-wide association analysis of seed germination percentage and germination index in Brassica napus L. under salt and drought stresses." Euphytica 213, no. 2: 1.
Xue-Ping Chen; Ling-Yun Jing; Jia Wang; Hong-Ju Jian; Jia-Qin Mei; Xin-Fu Xu; Jia-Na Li; Lie-Zhao Liu. Correlation Analysis of Sclerotinia Resistance with Lignin Content and Mono-mer G/S and Its QTL Mapping in Brassica napus L. Acta Agronomica Sinica 2017, 43, 1 .
AMA StyleXue-Ping Chen, Ling-Yun Jing, Jia Wang, Hong-Ju Jian, Jia-Qin Mei, Xin-Fu Xu, Jia-Na Li, Lie-Zhao Liu. Correlation Analysis of Sclerotinia Resistance with Lignin Content and Mono-mer G/S and Its QTL Mapping in Brassica napus L. Acta Agronomica Sinica. 2017; 43 (9):1.
Chicago/Turabian StyleXue-Ping Chen; Ling-Yun Jing; Jia Wang; Hong-Ju Jian; Jia-Qin Mei; Xin-Fu Xu; Jia-Na Li; Lie-Zhao Liu. 2017. "Correlation Analysis of Sclerotinia Resistance with Lignin Content and Mono-mer G/S and Its QTL Mapping in Brassica napus L." Acta Agronomica Sinica 43, no. 9: 1.
Kun Lu; Teng-Yue Wang; Xin-Fu Xu; Zhang-Lin Tang; Cun-Ming Qu; Bin He; Ying Liang; Jia-Na Li. Genome-Wide Association Analysis of Height of Podding and Thickness of Pod Canopy inBrassica napus. Acta Agronomica Sinica 2016, 42, 1 .
AMA StyleKun Lu, Teng-Yue Wang, Xin-Fu Xu, Zhang-Lin Tang, Cun-Ming Qu, Bin He, Ying Liang, Jia-Na Li. Genome-Wide Association Analysis of Height of Podding and Thickness of Pod Canopy inBrassica napus. Acta Agronomica Sinica. 2016; 42 (3):1.
Chicago/Turabian StyleKun Lu; Teng-Yue Wang; Xin-Fu Xu; Zhang-Lin Tang; Cun-Ming Qu; Bin He; Ying Liang; Jia-Na Li. 2016. "Genome-Wide Association Analysis of Height of Podding and Thickness of Pod Canopy inBrassica napus." Acta Agronomica Sinica 42, no. 3: 1.
Rapeseed contains glucosinolates, a toxic group of sulfur-containing glucosides, which play critical roles in defense against herbivores and microbes. However, the presence of glucosinolates in rapeseed reduces the value of the meal as feed for livestock. We performed association mapping of seed glucosinolate (GS) content using the 60K Brassica Infinium single nucleotide polymorphism (SNP) array in 520 oilseed rape accessions. A total of 11 peak SNPs significantly associated with GS content were detected in growing seasons of 2013 and 2014 and were located on B. napus chromosomes A08, A09, C03, and C09, respectively. Two associated regions of GS content covered by these markers were further verified, and three B. napus homologous genes involved in the biosynthesis and accumulation of GS were identified. These genes were multigene family members and were distributed on different chromosomes. Moreover, two genes (BnGRT2 and BnMYB28) associated with GS content were validated by the qRT-PCR analysis of their expression profiles. The further identification and functionalization of these genes will provide useful insight into the mechanism underlying GS biosynthesis and allocation in B. napus, and the associated SNPs markers could be helpful for molecular maker-assisted breeding for low seed GS in B. napus.
Cun-Min Qu; Shi-Meng Li; Xiu-Jian Duan; Jin-Hua Fan; Le-Dong Jia; Hui-Yan Zhao; Kun Lu; Jia-Na Li; Xin-Fu Xu; Rui Wang. Identification of Candidate Genes for Seed Glucosinolate Content Using Association Mapping in Brassica napus L. Genes 2015, 6, 1215 -1229.
AMA StyleCun-Min Qu, Shi-Meng Li, Xiu-Jian Duan, Jin-Hua Fan, Le-Dong Jia, Hui-Yan Zhao, Kun Lu, Jia-Na Li, Xin-Fu Xu, Rui Wang. Identification of Candidate Genes for Seed Glucosinolate Content Using Association Mapping in Brassica napus L. Genes. 2015; 6 (4):1215-1229.
Chicago/Turabian StyleCun-Min Qu; Shi-Meng Li; Xiu-Jian Duan; Jin-Hua Fan; Le-Dong Jia; Hui-Yan Zhao; Kun Lu; Jia-Na Li; Xin-Fu Xu; Rui Wang. 2015. "Identification of Candidate Genes for Seed Glucosinolate Content Using Association Mapping in Brassica napus L." Genes 6, no. 4: 1215-1229.
Kun Lu; Cun-Min Qu; Sha Li; Hui-Yan Zhao; Rui Wang; Xin-Fu Xu; Ying Liang; Jia-Na Li. Expression Analysis and eQTL Mapping ofBnTT3Gene inBrassica napusL. Acta Agronomica Sinica 2015, 41, 1 .
AMA StyleKun Lu, Cun-Min Qu, Sha Li, Hui-Yan Zhao, Rui Wang, Xin-Fu Xu, Ying Liang, Jia-Na Li. Expression Analysis and eQTL Mapping ofBnTT3Gene inBrassica napusL. Acta Agronomica Sinica. 2015; 41 (11):1.
Chicago/Turabian StyleKun Lu; Cun-Min Qu; Sha Li; Hui-Yan Zhao; Rui Wang; Xin-Fu Xu; Ying Liang; Jia-Na Li. 2015. "Expression Analysis and eQTL Mapping ofBnTT3Gene inBrassica napusL." Acta Agronomica Sinica 41, no. 11: 1.
Jie-Heng Huang; Wei Li; Cun-Min Qu; Lie-Zhao Liu; Xin-Fu Xu; Rui Wang; Jia-Na Li. Expression Characteristics of Key Genes in Lignin Pathway among Different Lodging Resistance Lines ofBrassica napusL.. Acta Agronomica Sinica 2013, 39, 1 .
AMA StyleJie-Heng Huang, Wei Li, Cun-Min Qu, Lie-Zhao Liu, Xin-Fu Xu, Rui Wang, Jia-Na Li. Expression Characteristics of Key Genes in Lignin Pathway among Different Lodging Resistance Lines ofBrassica napusL.. Acta Agronomica Sinica. 2013; 39 (8):1.
Chicago/Turabian StyleJie-Heng Huang; Wei Li; Cun-Min Qu; Lie-Zhao Liu; Xin-Fu Xu; Rui Wang; Jia-Na Li. 2013. "Expression Characteristics of Key Genes in Lignin Pathway among Different Lodging Resistance Lines ofBrassica napusL.." Acta Agronomica Sinica 39, no. 8: 1.
With 2 figures and 2 tables Abstract Brassica rapa (AA), which has a high level of genetic diversity, has been used to widen the genetic base of oilseed rape (B. napus L., AACC). However, the differences in the degree to which the genetic base of oilseed rape can be widened using different forms of B. rapa have not been well characterized. We used a strategy of virtual allopolyploid to investigate the genetic differences between the A subgenome of natural oilseed rape and different ecotypes of B. rapa. In this strategy, the genetic characteristics of the virtual allopolyploid lines are deduced from the genotypes of the parental lines which are established using molecular markers, without the need for interspecific hybridization. Three genotypes of B. oleracea and 56 accessions of B. rapa which originated from Europe, China and India were genotyped using simple sequence repeat markers, together with nine oilseed rape lines representing spring, semi‐winter and winter types. The genotypes of 168 virtual allopolyploid lines were derived from those of the parental lines and compared with those of the natural oilseed rape lines. Our data revealed that the virtual allopolyploid lines that were derived from Chinese semi‐winter B. rapa showed substantial genetic variation from natural oilseed rape. This indicates that Chinese semi‐winter B. rapa can be used effectively to broaden the germplasm of oilseed rape.
Jiaqin Mei; Ying Fu; Lunwen Qian; Xinfu Xu; Jiana Li; Wei Qian. Effectively widening the gene pool of oilseed rape (Brassica napus L.) by using Chinese B. rapa in a ‘virtual allopolyploid’ approach. Plant Breeding 2011, 130, 333 -337.
AMA StyleJiaqin Mei, Ying Fu, Lunwen Qian, Xinfu Xu, Jiana Li, Wei Qian. Effectively widening the gene pool of oilseed rape (Brassica napus L.) by using Chinese B. rapa in a ‘virtual allopolyploid’ approach. Plant Breeding. 2011; 130 (3):333-337.
Chicago/Turabian StyleJiaqin Mei; Ying Fu; Lunwen Qian; Xinfu Xu; Jiana Li; Wei Qian. 2011. "Effectively widening the gene pool of oilseed rape (Brassica napus L.) by using Chinese B. rapa in a ‘virtual allopolyploid’ approach." Plant Breeding 130, no. 3: 333-337.