High-strength low-alloy (HSLA) steel is widely used in automotive industry for reduction of consumption and emissions. The microstructure and mechanical properties of two automotive HSLA steels with different strength grades were systematically investigated in present study. Microstructural characterization was conducted using optical microscopy (OM), scanning electron microscopy (SEM), and electron backscatter diffraction (EBSD), while mechanical properties were evaluated with Vickers hardness tester and tensile tests. Both steels exhibited a ferrite matrix with spheroidized carbides/pearlites. However, Sample A displayed equiaxed ferrite grains with localized pearlite colonies, while Sample B featured pronounced elongated ferrite grains with a band structure. Tensile testing revealed that Sample B had higher ultimate tensile stress and yield stress compared to Sample A. Texture analysis indicated that both steels were dominated by α-fiber and γ-fiber textures, with minor θ-fiber texture, resulting in minimal mechanical anisotropy between the rolling direction (RD) and transverse direction (TD). The quantitative assessment of strengthening mechanisms, based on microstructural parameters and experimental data, revealed that grain boundary strengthening dominates, with dislocation strengthening also contributing significantly. This work provides the first comprehensive quantification of individual strengthening contributions in automotive HSLA steels, offering critical guidance for developing further higher-strength automotive steels.