Biofouling is a serious problem in marine aquaculture facilities, exerting several negative effects on cage structures. In this study, different materials of nets were placed in the Fujian Sea area of China, and the main biological adhesion species were determined. The drag force of different materials of fouled nets was studied by a physical test in a flume tank. The drag force coefficient of a clean polyethylene terephthalate (PET) net was 0.53. The drag force coefficients of ultrahigh-molecular-weight polyethylene (UHMWPE) and polyethylene (PE) nets were 161.2% and 133.5% higher, respectively, compared with those of PET nets. Crustaceans, mollusks, and algae were the main organisms that adhered to the nets. Compared with the clean nets, the drag force of PET, UHMWPE, and PE nets increased by 1.29–5.06 times, 1.11–2.85 times, and 0.55–2.46 times, respectively. Based on backpropagation (BP) neural network training, the relationship between biological characteristics (average adhesion thickness and density) and the drag force of three kinds of net materials was determined. The drag force of the biofouled net at various time points throughout the year can be predicted based on this model, which can guide the cleaning and maintenance of nets in cage structures.

The toxic effect and differences of Cr(VI) on superoxide dismutase (SOD) activity, MDA, and metallothionein (MT) content in the hepatopancreas and gill of Portunus trituberculatus were investigated during Cr(VI) enrichment (15 days) and release experiments (15 days). Results showed that the 1.50 and 0.30 mg/L test groups significantly exhibited higher SOD, MDA, and MT content in the hepatopancreas and gill compared with the control group after 15 days of enrichment (p < 0.05). After 15 days of Cr release, the SOD, MDA, and MT content in the hepatopancreas and gill of both test groups recovered to the normal level of the control group (p > 0.05). The gill of P. trituberculatus achieved the highest SOD activity, MDA, and MT content earlier than the hepatopancreas, but the highest values were lower. The gill showed a shorter recovery time than the hepatopancreas. We concluded that the gill of P. trituberculatus exhibited a more rapid response to, and recovery from, Cr(VI) exposure compared to the hepatopancreas, making it a more sensitive tissue for assessing Cr(VI) toxicity, though both tissues showed a capacity for recovery after the removal of the contaminant.

The clam Meretrix meretrix (Linnaeus, 1758) is a commercially significant bivalve species widely distributed along China’s coast. To investigate the differences among geoducks from different geographic populations, this study investigated the geographical variations in morphology and key physiological traits among three populations spanning a latitudinal gradient: Liaoning Dandong (north), Jiangsu Rudong (center), and Guangxi Qinzhou (south). We analyzed nine morphological traits and measured physiological indicators—including filtration rate, feeding rate, oxygen consumption, ammonia excretion, and burrowing behavior—at two temperatures (18 °C and 20 °C). The results revealed significant morphological differentiation among the populations. Physiologically, the Guangxi population demonstrated superior adaptation to warmer conditions, exhibiting the highest feeding and metabolic rates at 20 °C. In contrast, the Liaoning population performed better at the lower temperature of 18 °C, while the Jiangsu population showed intermediate characteristics. Furthermore, burrowing capacity varied significantly, with the southern population having the highest burrowing rate. These findings demonstrate clear population-specific adaptations to local thermal environments, likely driven by long-term acclimatization. This study provides a crucial scientific basis for the conservation of genetic resources and informs regionalized aquaculture strategies for M. meretrix, emphasizing the importance of selecting locally adapted populations for cultivation.

(1) Exploring the potential impacts of Mactra veneriformis and Ruditapes philippinarum on the aquaculture of Meretrix meretrix in the tidal flat aquaculture zone is significant for the optimization of bivalve aquaculture structure. (2) In this study, we systematically compared the differences in feeding, metabolic physiological indicators, and energy budgets of the three bivalve species in the M. meretrix aquaculture zone of Rudong County, Jiangsu Province, under the feeding conditions of Isochrysis zhanjiangensis and Platymonas helgolandica. (3) The results indicated that feeding with I. zhanjiangensis could significantly improve the feeding efficiency of the three bivalve species, and M. veneriformis showed the strongest feeding ability. Under I. zhanjiangensis feeding conditions, the clearance rate [1.373 ± 0.177 L/(g·h)] and ingestion rate [9.803 ± 1.264 mg/(g·h)] of M. veneriformis were significantly higher than those of M. meretrix [clearance rate: 0.716 ± 0.046 L/(g·h); ingestion rate: 5.112 ± 0.328 mg/(g·h)] and R. philippinarum [clearance rate: 0.609 ± 0.101 L/(g·h); ingestion rate: 4.351 ± 0.725 mg/(g·h)] (p < 0.05). In terms of energy allocation, the scope for growth (SFG) of M. veneriformis [133.050 ± 15.705 J/(g·h)] was significantly higher than that of the other two bivalve species. M. meretrix [70.550 ± 2.133 J/(g·h)] had the highest energy utilization efficiency, while R. philippinarum [53.688 ± 7.548 J/(g·h)] had the lowest SFG due to high excretion losses. (4) In conclusion, M. veneriformis shows high production potential in the aquaculture system, and its potential impact on M. meretrix is greater than that of R. philippinarum, thus requiring close attention in aquaculture management.

This study evaluated the endpoint biochemical responses and associated physiological status of rainbow trout (Oncorhynchus mykiss) of different body sizes during salinity acclimation under varying temperature and salinity conditions. An L₉ (3⁴) orthogonal design was applied using three nominal body-size levels (100, 250, and 500 g), three temperatures (10, 15, and 20 °C), and three salinities (10, 20, and 30‰) over 60 days. Hepatic superoxide dismutase (SOD), alkaline phosphatase (ALP), and acid phosphatase (ACP) activities and plasma malondialdehyde (MDA), urea nitrogen (BUN), ammonia, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were measured. For inferential analysis, the three fish sampled from each tank were averaged to obtain one tank-level value, yielding 18 tank-level observations. Tank-level main-effects analysis showed that body size was significantly associated with ALP, ACP, MDA, BUN, ammonia, ALT, and AST. Temperature was significantly associated with SOD, ACP, MDA, BUN, and AST, whereas salinity was significantly associated with MDA, ALT, and AST. MDA and AST were the only endpoints associated with all three factors. The biochemical responses were pathway-specific rather than uniformly directional across treatment combinations. Because interactions could not be independently estimated in the L₉ design, the identified main effects should be interpreted as orthogonal screening associations. Overall, body-size class was associated with the broadest range of endpoints and should be incorporated into future experimental assessments of rainbow trout within the tested salinity range of 10–30‰.