Journal of Medical Internet Research - International Scientific Journal for Medical Research, Information and Communication on the Internet #Preprint #PeerReviewMe: Warning: This is a unreviewed preprint. Readers are warned that the document has not been peer-reviewed by expert/patient reviewers or an academic editor, may contain misleading claims, and is likely to undergo changes before final publication, if accepted, or may have been rejected/withdrawn. Readers with interest and expertise are encouraged to sign up as peer-reviewer, if the paper is within an open peer-review period. Please cite this preprint only for review purposes or for grant applications and CVs (if you are the author). Background: Smartphone diet-tracking applications may help individuals lose weight, manage chronic conditions, and understand dietary patterns, however, the usabilities and functionalities of these apps have not been well studied. Objective: The aim of this study was to review the usability of current iOS and Android diet-tracking apps, the degree to which app features align with behavior change constructs, and to assess variations between apps in nutrient coding. Methods: The top seven diet-tracking apps were identified from the iOS iTunes and Android Play online stores, downloaded and used over a 2-week period. Each app was independently scored by researchers using the System of Usability Scale (SUS), and features were compared to the domains in an integrated behavior change theory framework: the Theoretical Domains Framework (TDF). An estimated 3-day food diary was completed using each app and food items were entered into USDA Food Composition Databases to evaluate their differences in nutrient data against the USDA reference. Results: Of the apps that were reviewed, LifeSum scored the highest average SUS score of 89.2, while MyDietCoach scored the lowest of 46.7. There were some variations in features noted between Android and iOS versions of the same apps, mainly for MyDietCoach, which affected SUS scoring. App features varied considerably, yet all of the apps had features consistent with Beliefs about Capabilities, and thus have the potential to promote self-efficacy by helping individuals track their diet and progress towards goals. None of the apps allowed for tracking of emotional factors that may be associated with diet patterns. The presence of behavior change domain features tended to be weakly correlated with greater usability (R2 ranging from 0 to 0.396). The exception to this was features related to the Reinforcement domain, which were correlated with less usability. Comparing the apps to the USDA reference for a 3-day diet, the average differences were 1.4 % for calories, 1.0 % for carbohydrates, 10.4% for protein, and -6.5% for fat. Conclusions: Almost all reviewed diet tracking apps score well in usability, utilize a variety of behavior change constructs, and accurately code calories and carbohydrates, allowing them to play a potential role in dietary intervention studies.