This page has only limited features, please log in for full access.

Dr. Yan Peng
Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University

Basic Info

Basic Info is private.

Research Keywords & Expertise

0 Plant Physiology
0 gene function
0 abiotic stress
0 gene clone
0 white clover

Fingerprints

white clover
abiotic stress
gene function

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 08 July 2021 in Antioxidants
Reads 0
Downloads 0

Persistent high temperature decreases the yield and quality of crops, including many important herbs. White clover (Trifolium repens) is a perennial herb with high feeding and medicinal value, but is sensitive to temperatures above 30 °C. The present study was conducted to elucidate the impact of changes in endogenous γ-aminobutyric acid (GABA) level by exogenous GABA pretreatment on heat tolerance of white clover, associated with alterations in endogenous hormones, antioxidant metabolism, and aquaporin-related gene expression in root and leaf of white clover plants under high-temperature stress. Our results reveal that improvement in endogenous GABA level in leaf and root by GABA pretreatment could significantly alleviate the damage to white clover during high-temperature stress, as demonstrated by enhancements in cell membrane stability, photosynthetic capacity, and osmotic adjustment ability, as well as lower oxidative damage and chlorophyll loss. The GABA significantly enhanced gene expression and enzyme activities involved in antioxidant defense, including superoxide dismutase, catalase, peroxidase, and key enzymes of the ascorbic acid–glutathione cycle, thus reducing the accumulation of reactive oxygen species and the oxidative injury to membrane lipids and proteins. The GABA also increased endogenous indole-3-acetic acid content in roots and leaves and cytokinin content in leaves, associated with growth maintenance and reduced leaf senescence under heat stress. The GABA significantly upregulated the expression of PIP1-1 and PIP2-7 in leaves and the TIP2-1 expression in leaves and roots under high temperature, and also alleviated the heat-induced inhibition of PIP1-1, PIP2-2, TIP2-2, and NIP1-2 expression in roots, which could help to improve the water transportation and homeostasis from roots to leaves. In addition, the GABA-induced aquaporins expression and decline in endogenous abscisic acid level could improve the heat dissipation capacity through maintaining higher stomatal opening and transpiration in white clovers under high-temperature stress.

ACS Style

Hongyin Qi; Dingfan Kang; Weihang Zeng; Muhammad Jawad Hassan; Yan Peng; Xinquan Zhang; Yan Zhang; Guangyan Feng; Zhou Li. Alterations of Endogenous Hormones, Antioxidant Metabolism, and Aquaporin Gene Expression in Relation to γ-Aminobutyric Acid-Regulated Thermotolerance in White Clover. Antioxidants 2021, 10, 1099 .

AMA Style

Hongyin Qi, Dingfan Kang, Weihang Zeng, Muhammad Jawad Hassan, Yan Peng, Xinquan Zhang, Yan Zhang, Guangyan Feng, Zhou Li. Alterations of Endogenous Hormones, Antioxidant Metabolism, and Aquaporin Gene Expression in Relation to γ-Aminobutyric Acid-Regulated Thermotolerance in White Clover. Antioxidants. 2021; 10 (7):1099.

Chicago/Turabian Style

Hongyin Qi; Dingfan Kang; Weihang Zeng; Muhammad Jawad Hassan; Yan Peng; Xinquan Zhang; Yan Zhang; Guangyan Feng; Zhou Li. 2021. "Alterations of Endogenous Hormones, Antioxidant Metabolism, and Aquaporin Gene Expression in Relation to γ-Aminobutyric Acid-Regulated Thermotolerance in White Clover." Antioxidants 10, no. 7: 1099.

Journal article
Published: 19 May 2021 in Plant Physiology and Biochemistry
Reads 0
Downloads 0

S-adenosylmethionine decarboxylase (SAMDC) mediates the biosynthesis of polyamines (PAs) and plays a positive role in plants’ response to adversity stress tolerance. In this study, we isolated a SAMDC gene from white clover, which is located in mitochondria. It was strongly induced when white clover exposed to drought (15% PEG6000), salinity (200 mM NaCl), 20 μM spermidine, 100 μM abscisic acid, and 10 mM H2O2, especially in leaves. The INVSc1 yeast introduced with TrSAMDC1 had tolerance to drought, salt, and oxidative stress. Overexpression of TrSAMDC1 in Arabidopsis showed higher fresh weight and dry weight under drought and salt treatment and without growth inhibition under normal conditions. Leaf senescence induced by drought and saline was further delayed in transgenic plants, regardless of cultivation in 1/2 MS medium and soil. During drought and salt stress, transgenic plants exhibited a significant increase in relative water content, maximum photosynthesis efficiency (Fv/Fm), performance index on the absorption basis (PIABS), activities of antioxidant protective enzymes such as SOD, POD, CAT, and APX, and a significant decrease in accumulation of MDA and H2O2 as compared to the WT. The concentrations of total PAs, putrescine, spermidine, and spermidine in transgenic lines were higher in transgenic plants than in WT under normal and drought conditions. These results suggested that TrSAMDC1 could effectively mitigate abiotic stresses without the expense of production and be a potential candidate gene for improving the drought and salt resistance of crops.

ACS Style

Tong Jia; Jieru Hou; Muhammad Zafar Iqbal; Youzhi Zhang; Bizhen Cheng; Huahao Feng; Zhou Li; Lin Liu; Jiqiong Zhou; Guangyan Feng; Gang Nie; Xiao Ma; Wei Liu; Yan Peng. Overexpression of the white clover TrSAMDC1 gene enhanced salt and drought resistance in Arabidopsis thaliana. Plant Physiology and Biochemistry 2021, 165, 147 -160.

AMA Style

Tong Jia, Jieru Hou, Muhammad Zafar Iqbal, Youzhi Zhang, Bizhen Cheng, Huahao Feng, Zhou Li, Lin Liu, Jiqiong Zhou, Guangyan Feng, Gang Nie, Xiao Ma, Wei Liu, Yan Peng. Overexpression of the white clover TrSAMDC1 gene enhanced salt and drought resistance in Arabidopsis thaliana. Plant Physiology and Biochemistry. 2021; 165 ():147-160.

Chicago/Turabian Style

Tong Jia; Jieru Hou; Muhammad Zafar Iqbal; Youzhi Zhang; Bizhen Cheng; Huahao Feng; Zhou Li; Lin Liu; Jiqiong Zhou; Guangyan Feng; Gang Nie; Xiao Ma; Wei Liu; Yan Peng. 2021. "Overexpression of the white clover TrSAMDC1 gene enhanced salt and drought resistance in Arabidopsis thaliana." Plant Physiology and Biochemistry 165, no. : 147-160.

Journal article
Published: 13 November 2020 in Plants
Reads 0
Downloads 0

Heavy metal stress is a leading environmental issue reducing crop growth and productivity, particularly in arid and semi-arid agro-ecological zones. Cadmium (Cd), a non-redox heavy metal, can indirectly increase the production of reactive oxygen species (ROS), inducing cell death. A pot experiment was conducted to investigate the effects of different concentrations of Cd (0, 5, 25, 50, 100 µM) on physiological and biochemical parameters in two sorghum (Sorghum bicolor L.) cultivars: JS-2002 and Chakwal Sorghum. The results showed that various concentrations of Cd significantly increased the Cd uptake in both cultivars; however, the uptake was higher in JS-2002 compared to Chakwal Sorghum in leaf, stem and root. Regardless of the cultivars, there was a higher accumulation of the Cd in roots than in shoots. The Cd stress significantly reduced the growth and increased the electrolyte leakage (EL), hydrogen peroxide (H2O2) concentration and malondialdehyde (MDA) content in both cultivars, but the Chakwal Sorghum showed more pronounced oxidative damage than the JS-2002, as reflected by higher H2O2, MDA and EL. Moreover, Cd stress, particularly 50 µM and 100 µM, decreased the activity of different antioxidant enzymes, including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). However, the JS-2002 exhibited higher SOD, POD and CAT activities than the Chakwal Sorghum under different Cd-levels. These findings revealed that JS-2002 had a stronger Cd enrichment capacity and also exhibited a better tolerance to Cd stress due to its efficient antioxidant defense system than Chakwal Sorghum. The present study provides the available information about Cd enrichment and tolerance in S. bicolor, which is used as an important agricultural crop for livestock feed in arid and semi-arid regions.

ACS Style

Muhammad Jawad Hassan; Muhammad Ali Raza; Sana Ur Rehman; Muhammad Ansar; Harun Gitari; Imran Khan; Muhammad Wajid; Mukhtar Ahmed; Ghulam Abbas Shah; Yan Peng; Zhou Li. Effect of Cadmium Toxicity on Growth, Oxidative Damage, Antioxidant Defense System and Cadmium Accumulation in Two Sorghum Cultivars. Plants 2020, 9, 1575 .

AMA Style

Muhammad Jawad Hassan, Muhammad Ali Raza, Sana Ur Rehman, Muhammad Ansar, Harun Gitari, Imran Khan, Muhammad Wajid, Mukhtar Ahmed, Ghulam Abbas Shah, Yan Peng, Zhou Li. Effect of Cadmium Toxicity on Growth, Oxidative Damage, Antioxidant Defense System and Cadmium Accumulation in Two Sorghum Cultivars. Plants. 2020; 9 (11):1575.

Chicago/Turabian Style

Muhammad Jawad Hassan; Muhammad Ali Raza; Sana Ur Rehman; Muhammad Ansar; Harun Gitari; Imran Khan; Muhammad Wajid; Mukhtar Ahmed; Ghulam Abbas Shah; Yan Peng; Zhou Li. 2020. "Effect of Cadmium Toxicity on Growth, Oxidative Damage, Antioxidant Defense System and Cadmium Accumulation in Two Sorghum Cultivars." Plants 9, no. 11: 1575.

Journal article
Published: 10 October 2020 in International Journal of Molecular Sciences
Reads 0
Downloads 0

γ-Aminobutyric acid (GABA) plays an important role in regulating stress tolerance in plants. Purposes of this study was to determine the effect of an exogenous supply of GABA on tolerance to water stress in creeping bentgrass (Agrostis stolonifera), and further reveal the GABA-induced key mechanisms related to water balance, nitrogen (N) metabolism and nitric oxide (NO) production in response to water stress. Plants were pretreated with or without 0.5 mM GABA solution in the roots for 3 days, and then subjected to water stress induced by –0.52 MPa polyethylene glycol 6000 for 12 days. The results showed that water stress caused leaf water deficit, chlorophyll (Chl) loss, oxidative damage (increases in superoxide anion, hydrogen peroxide, malondialdehyde, and protein carbonyl content), N insufficiency, and metabolic disturbance. However, the exogenous addition of GABA significantly increased endogenous GABA content, osmotic adjustment and antioxidant enzyme activities (superoxide dismutase, catalase, dehydroascorbate reductase, glutathione reductase and monodehydroascorbate reductase), followed by effectively alleviating water stress damage, including declines in oxidative damage, photoinhibition, and water and Chl loss. GABA supply not only provided more available N, but also affected N metabolism through activating nitrite reductase and glutamine synthetase activities under water stress. The supply of GABA did not increase glutamate content and glutamate decarboxylase activity, but enhanced glutamate dehydrogenase activity, which might indicate that GABA promoted the conversion and utilization of glutamate for maintaining Chl synthesis and tricarboxylic acid cycle when creeping bentgrass underwent water stress. In addition, GABA-induced NO production, depending on nitrate reductase and NO-associated protein pathways, could be associated with the enhancement of antioxidant defense. Current findings reveal the critical role of GABA in regulating signal transduction and metabolic homeostasis in plants under water-limited condition.

ACS Style

Mingyan Tang; Zhou Li; Ling Luo; Bizhen Cheng; Youzhi Zhang; Weihang Zeng; Yan Peng. Nitric Oxide Signal, Nitrogen Metabolism, and Water Balance Affected by γ-Aminobutyric Acid (GABA) in Relation to Enhanced Tolerance to Water Stress in Creeping Bentgrass. International Journal of Molecular Sciences 2020, 21, 7460 .

AMA Style

Mingyan Tang, Zhou Li, Ling Luo, Bizhen Cheng, Youzhi Zhang, Weihang Zeng, Yan Peng. Nitric Oxide Signal, Nitrogen Metabolism, and Water Balance Affected by γ-Aminobutyric Acid (GABA) in Relation to Enhanced Tolerance to Water Stress in Creeping Bentgrass. International Journal of Molecular Sciences. 2020; 21 (20):7460.

Chicago/Turabian Style

Mingyan Tang; Zhou Li; Ling Luo; Bizhen Cheng; Youzhi Zhang; Weihang Zeng; Yan Peng. 2020. "Nitric Oxide Signal, Nitrogen Metabolism, and Water Balance Affected by γ-Aminobutyric Acid (GABA) in Relation to Enhanced Tolerance to Water Stress in Creeping Bentgrass." International Journal of Molecular Sciences 21, no. 20: 7460.

Journal article
Published: 18 September 2020 in Molecules
Reads 0
Downloads 0

γ-Aminobutyric acid (GABA) participates in the regulation of adaptability to abiotic stress in plants. The objectives of this study were to investigate the effects of GABA priming on improving thermotolerance in creeping bentgrass (Agrostis stolonifera) based on analyses of physiology and proteome using iTRAQ technology. GABA-treated plants maintained significantly higher endogenous GABA content, photochemical efficiency, performance index on absorption basis, membrane stability, and osmotic adjustment (OA) than untreated plants during a prolonged period of heat stress (18 days), which indicated beneficial effects of GABA on alleviating heat damage. Protein profiles showed that plants were able to regulate some common metabolic processes including porphyrin and chlorophyll metabolism, glutathione metabolism, pyruvate metabolism, carbon fixation, and amino acid metabolism for heat acclimation. It is noteworthy that the GABA application particularly regulated arachidonic acid metabolism and phenylpropanoid biosynthesis related to better thermotolerance. In response to heat stress, the GABA priming significantly increased the abundances of Cu/ZnSOD and APX4 that were consistent with superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities. The GABA-upregulated proteins in relation to antioxidant defense (Cu/ZnSOD and APX4) for the reactive oxygen species scavenging, heat shock response (HSP90, HSP70, and HSP16.9) for preventing denatured proteins aggregation, stabilizing abnormal proteins, promoting protein maturation and assembly, sugars, and amino acids metabolism (PFK5, ATP-dependent 6-phosphofructokinase 5; FK2, fructokinase 2; BFRUCT, β-fructofuranosidase; RFS2, galactinol-sucrose galactosyltransferase 2; ASN2, asparagine synthetase 2) for OA and energy metabolism, and transcription factor (C2H2 ZNF, C2H2 zinc-finger protein) for the activation of stress-defensive genes could play vital roles in establishing thermotolerance. Current findings provide an illuminating insight into the new function of GABA on enhancing adaptability to heat stress in plants.

ACS Style

Zhou Li; Weihang Zeng; Bizhen Cheng; Ting Huang; Yan Peng; Xinquan Zhang. γ-Aminobutyric Acid Enhances Heat Tolerance Associated with the Change of Proteomic Profiling in Creeping Bentgrass. Molecules 2020, 25, 4270 .

AMA Style

Zhou Li, Weihang Zeng, Bizhen Cheng, Ting Huang, Yan Peng, Xinquan Zhang. γ-Aminobutyric Acid Enhances Heat Tolerance Associated with the Change of Proteomic Profiling in Creeping Bentgrass. Molecules. 2020; 25 (18):4270.

Chicago/Turabian Style

Zhou Li; Weihang Zeng; Bizhen Cheng; Ting Huang; Yan Peng; Xinquan Zhang. 2020. "γ-Aminobutyric Acid Enhances Heat Tolerance Associated with the Change of Proteomic Profiling in Creeping Bentgrass." Molecules 25, no. 18: 4270.

Journal article
Published: 05 August 2020 in Plants
Reads 0
Downloads 0

White clover (Trifolium repens L.) is a widely cultivated cool-season perennial forage legume in temperate grassland systems. Many studies have analyzed the gene expression in this grass species using quantitative real-time reverse transcription PCR (qRT-PCR). The selection of stable reference genes for qRT-PCR is crucial. However, there was no detailed study on reference genes in different tissues of white clover under various abiotic stress conditions. Herein, 14 candidate reference genes (ACT7, ACT101, TUA1109, TUB, CYP, 60SrRNA, UBQ, E3, GAPDH1, GAPDH2, PP2A, BAM3, SAMDC, and ABC) were selected and analyzed by four programs (GeNorm, NormFinder, BestKeeper, and RefFinder). Samples were taken from two tissues (leaves and roots) under five different abiotic stresses (drought, salt, heat, cold, and heavy metal stress). Our results showed that 60SrRNA and ACT101 were the two top-ranked genes for all samples. Under various experimental conditions, the most stable gene was different; however, SAMDC, UBQ, 60SrRNA, and ACT101 were always top ranked. The most suitable reference genes should be selected according to different plant tissues and growth conditions. Validation of these reference genes by expression analysis of Cyt-Cu/Zn SOD and CAT confirmed their reliability. Our study will benefit the subsequent research of gene function in this species.

ACS Style

Qi Pu; Zhou Li; Gang Nie; Jiqiong Zhou; Lin Liu; Yan Peng. Selection and Validation of Reference Genes for Quantitative Real-Time PCR in White Clover (Trifolium repens L.) Involved in Five Abiotic Stresses. Plants 2020, 9, 996 .

AMA Style

Qi Pu, Zhou Li, Gang Nie, Jiqiong Zhou, Lin Liu, Yan Peng. Selection and Validation of Reference Genes for Quantitative Real-Time PCR in White Clover (Trifolium repens L.) Involved in Five Abiotic Stresses. Plants. 2020; 9 (8):996.

Chicago/Turabian Style

Qi Pu; Zhou Li; Gang Nie; Jiqiong Zhou; Lin Liu; Yan Peng. 2020. "Selection and Validation of Reference Genes for Quantitative Real-Time PCR in White Clover (Trifolium repens L.) Involved in Five Abiotic Stresses." Plants 9, no. 8: 996.

Journal article
Published: 09 April 2020 in Plants
Reads 0
Downloads 0

Trifolium L., which belongs to the IR lacking clade (IRLC), is one of the largest genera in the Leguminosae and contains several economically important fodder species. Here, we present whole chloroplast (cp) genome sequencing and annotation of two important annual grasses, Trifolium alexandrinum (Egyptian clover) and T. resupinatum (Persian clover). Abundant single nucleotide polymorphisms (SNPs) and insertions/deletions (In/Dels) were discovered between those two species. Global alignment of T. alexandrinum and T. resupinatum to a further thirteen Trifolium species revealed a large amount of rearrangement and repetitive events in these fifteen species. As hypothetical cp open reading frame (ORF) and RNA polymerase subunits, ycf1 and rpoC2 in the cp genomes both contain vast repetitive sequences and observed high Pi values (0.7008, 0.3982) between T. alexandrinum and T. resupinatum. Thus they could be considered as the candidate genes for phylogenetic analysis of Trifolium species. In addition, the divergence time of those IR lacking Trifolium species ranged from 84.8505 Mya to 4.7720 Mya. This study will provide insight into the evolution of Trifolium species.

ACS Style

Yanli Xiong; Yi Xiong; Jun He; Qingqing Yu; Junming Zhao; Xiong Lei; Zhixiao Dong; Jian Yang; Yan Peng; Xinquan Zhang; Xiao Ma. The Complete Chloroplast Genome of Two Important Annual Clover Species, Trifolium alexandrinum and T. resupinatum: Genome Structure, Comparative Analyses and Phylogenetic Relationships with Relatives in Leguminosae. Plants 2020, 9, 478 .

AMA Style

Yanli Xiong, Yi Xiong, Jun He, Qingqing Yu, Junming Zhao, Xiong Lei, Zhixiao Dong, Jian Yang, Yan Peng, Xinquan Zhang, Xiao Ma. The Complete Chloroplast Genome of Two Important Annual Clover Species, Trifolium alexandrinum and T. resupinatum: Genome Structure, Comparative Analyses and Phylogenetic Relationships with Relatives in Leguminosae. Plants. 2020; 9 (4):478.

Chicago/Turabian Style

Yanli Xiong; Yi Xiong; Jun He; Qingqing Yu; Junming Zhao; Xiong Lei; Zhixiao Dong; Jian Yang; Yan Peng; Xinquan Zhang; Xiao Ma. 2020. "The Complete Chloroplast Genome of Two Important Annual Clover Species, Trifolium alexandrinum and T. resupinatum: Genome Structure, Comparative Analyses and Phylogenetic Relationships with Relatives in Leguminosae." Plants 9, no. 4: 478.

Journal article
Published: 08 April 2020 in BMC Plant Biology
Reads 0
Downloads 0

Background Auxin may have a positive effect on plants under drought stress. White clover is widely cultivated and often prone to water shortages. In the present study, we investigated the effects of exogenous indole − 3-acetic acid (IAA) on growth and physiological changes of white clover under drought stress condition. The contents of endogenous IAA and other hormones including ABA, CTK, JA, GA, IAA, and SA were assayed. Moreover, expressions of auxin-responsive genes, drought-responsive genes and leaf senescence-associated genes were detected in response to exogenous IAA. Results Compared to control, drought stress alone significantly diminished stem dry weigh, relative water content (RWC) and total chlorophyll content (Chl). Exogenous IAA treatment significantly increased RWC and Chl, whereas L-AOPP treatment drastically decreased stem dry weight, RWC and Chl under drought stress condition. Additionally, exogenous IAA treatment significantly increased ABA content and JA content, up-regulated expression of auxin responsive genes (GH3.1, GH3.9, IAA8), drought stress responsive genes (bZIP11, DREB2, MYB14, MYB48, WRKY2, WRKY56, WRKY108715 and RD22), and down-regulated expressions of auxin-responding genes (GH3.3, GH3.6, IAA27) and leaf senescence genes (SAG101 and SAG102) in the presence of PEG. Contrarily, L-AOPP treatment significantly reduced contents of ABA, GA3 and JA, down-regulated expressions of GH3.1, GH3.9, IAA8, bZIP11, DREB2, MYB14, MYB48, WRKY2, WRKY56, WRKY108715, ERD and RD22, and up-regulated SAG101 and SAG102. Conclusions Exogenous IAA improved drought tolerance of white clover possibly due to endogenous plant hormone concentration changes and modulation of genes involving in drought stress response and leaf senescence. These results provided useful information to understand mechanisms of IAA improved drought tolerance in white clover.

ACS Style

Youzhi Zhang; Yaping Li; Muhammad Jawad Hassan; Zhou Li; Yan Peng. Indole-3-acetic acid improves drought tolerance of white clover via activating auxin, abscisic acid and jasmonic acid related genes and inhibiting senescence genes. BMC Plant Biology 2020, 20, 1 -12.

AMA Style

Youzhi Zhang, Yaping Li, Muhammad Jawad Hassan, Zhou Li, Yan Peng. Indole-3-acetic acid improves drought tolerance of white clover via activating auxin, abscisic acid and jasmonic acid related genes and inhibiting senescence genes. BMC Plant Biology. 2020; 20 (1):1-12.

Chicago/Turabian Style

Youzhi Zhang; Yaping Li; Muhammad Jawad Hassan; Zhou Li; Yan Peng. 2020. "Indole-3-acetic acid improves drought tolerance of white clover via activating auxin, abscisic acid and jasmonic acid related genes and inhibiting senescence genes." BMC Plant Biology 20, no. 1: 1-12.

Journal article
Published: 07 April 2020 in Plants
Reads 0
Downloads 0

Salinity is a major abiotic stress which limits crop production, especially under rainfed conditions. Selenium (Se), as an important micronutrient, plays a vital role in mitigating detrimental effects of different abiotic stresses. The objective of this research was to examine the effect of Se fertilization on black gram (Vigna mungo) under salt stress. Our results showed that salt stress (100 mM NaCl) in leaves significantly induced oxidative damage and caused a decline in relative water content, chlorophyll (Chl), stomatal conductance (gs), photochemical efficiency (Fv/Fm), sucrose, and reducing sugars. A low dose of Se (1.5 ppm) significantly reduced hydrogen peroxide content, malondialdehyde formation, cell membrane damage, and also improved antioxidative enzyme activities, including superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase, and glutathione peroxidase under salt stress. Se-treated plants exhibited higher Chl, gs, Fv/Fm, sucrose, and reducing sugars than untreated plants in response to salt stress. In addition, Se application enhanced Se uptake and reduced Na+ uptake, but Cl− remained unaffected. Our results indicated that a low dose of Se effectively alleviated salt damage via inhibition of Na+ uptake and enhanced antioxidant defense resulting in a significant decrease in oxidative damage, and maintained gaseous exchange and PS II function for sucrose and reducing sugars accumulation in black gram.

ACS Style

Muhammad Jawad Hassan; Muhammad Ali Raza; Imran Khan; Tehseen Ahmad Meraj; Mukhtar Ahmed; Ghulam Abbas Shah; Muhammad Ansar; Samrah Afzal Awan; Nanak Khan; Nasir Iqbal; Yan Peng; Zhou Li. Selenium and Salt Interactions in Black Gram (Vigna mungo L): Ion Uptake, Antioxidant Defense System, and Photochemistry Efficiency. Plants 2020, 9, 467 .

AMA Style

Muhammad Jawad Hassan, Muhammad Ali Raza, Imran Khan, Tehseen Ahmad Meraj, Mukhtar Ahmed, Ghulam Abbas Shah, Muhammad Ansar, Samrah Afzal Awan, Nanak Khan, Nasir Iqbal, Yan Peng, Zhou Li. Selenium and Salt Interactions in Black Gram (Vigna mungo L): Ion Uptake, Antioxidant Defense System, and Photochemistry Efficiency. Plants. 2020; 9 (4):467.

Chicago/Turabian Style

Muhammad Jawad Hassan; Muhammad Ali Raza; Imran Khan; Tehseen Ahmad Meraj; Mukhtar Ahmed; Ghulam Abbas Shah; Muhammad Ansar; Samrah Afzal Awan; Nanak Khan; Nasir Iqbal; Yan Peng; Zhou Li. 2020. "Selenium and Salt Interactions in Black Gram (Vigna mungo L): Ion Uptake, Antioxidant Defense System, and Photochemistry Efficiency." Plants 9, no. 4: 467.

Preprint content
Published: 24 March 2020
Reads 0
Downloads 0

Background: Auxin may have a positive effect on plants under drought stress. White clover is widely cultivated and often prone to water shortages. In the present study, we investigated the effects of exogenous indole -3-acetic acid (IAA) on growth and physiological changes of white clover under drought stress condition. The contents of endogenous IAA and other hormones including ABA, CTK, JA, GA, IAA, and SA were assayed. Moreover, expressions of auxin-responsive genes, drought-responsive genes and leaf senescence-associated genes were detected in response to exogenous IAA. Results: Compared to control, drought stress alone significantly diminished stem dry weigh, relative water content (RWC) and total chlorophyll content (Chl). Exogenous IAA treatment significantly increased RWC and Chl, whereas L-AOPP treatment drastically decreased stem dry weight, RWC and Chl under drought stress condition. Additionally, exogenous IAA treatment significantly increased ABA content and JA content, up-regulated expression of auxin responsive genes ( GH3.1 , GH3.9 , IAA8 ), drought stress responsive genes ( bZIP11 , DREB2 , MYB14 , MYB48 , WRKY2 , WRKY56 , WRKY108715 and RD22 ), and down-regulated expressions of auxin-responding genes ( GH3.3 , GH3.6 , IAA27 ) and leaf senescence genes ( SAG101 and SAG102 ) in the presence of PEG. Contrarily, L-AOPP treatment significantly reduced contents of ABA, GA3 and JA, down-regulated expressions of GH3.1 , GH3.9 , IAA8 , bZIP11 , DREB2 , MYB14 , MYB48 , WRKY2 , WRKY56 , WRKY108715 , ERD and RD22 , and up-regulated SAG101 and SAG102 . Conclusions: Exogenous IAA improved drought tolerance of white clover possibly due to endogenous plant hormone concentration changes and modulation of genes involving in drought stress response and leaf senescence. These results provided useful information to understand mechanisms of IAA improved drought tolerance in white clover.

ACS Style

Youzhi Zhang; Yaping Li; Muhammad Jawad Hassan; Zhou Li; Yan Peng. Indole -3-acetic acid improves drought tolerance of white clover via activating auxin, abscisic acid and jasmonic acid related genes and inhibiting senescence genes. 2020, 1 .

AMA Style

Youzhi Zhang, Yaping Li, Muhammad Jawad Hassan, Zhou Li, Yan Peng. Indole -3-acetic acid improves drought tolerance of white clover via activating auxin, abscisic acid and jasmonic acid related genes and inhibiting senescence genes. . 2020; ():1.

Chicago/Turabian Style

Youzhi Zhang; Yaping Li; Muhammad Jawad Hassan; Zhou Li; Yan Peng. 2020. "Indole -3-acetic acid improves drought tolerance of white clover via activating auxin, abscisic acid and jasmonic acid related genes and inhibiting senescence genes." , no. : 1.

Preprint content
Published: 27 January 2020
Reads 0
Downloads 0

Auxin plays an important role in regulating plant development. To idenfity what effects IAA has in white clover under drought stress and how IAA regulates white clover response to drought. Relative water content, chlorophyll content, six phytohormones content, expression of gene-related to IAA signal perception and synthesis, transcription factor genes related to drought-resistance and senescence genes were investigated in white clover pretreated with 1µM exogenous IAA or 100µM L-AOPP (inhibitor of IAA synthesis) under 15% polyethylene glycol-6000 (PEG-6000). Compared to control, drought stress significantly diminished stem dry weight (g/10 plant), relative water content (RWC, %) and total chlorophyll content (Chl, mg/g). Exogenous IAA significantly increased RWC and Chl, however, L-AOPP decreased stem dry weight, RWC and Chl compared with direct drought treatment. Besides that, compared with direct drought set, exogenous IAA significantly improved ABA content and JA content, up-regulated expression levels of some auxin-responsive genes ( GH3.1 , GH3.9 , IAA8 ) , transcription factor genes ( bZIP11 , DREB2 , MYB14 , MYB48 , WRKY2 , WRKY56 , WRKY108715 ) related to drought-resistance and drought resistance gene ( RD22 ). Meanwhile, exogenous IAA down-regulated expression levels of several auxin-responsive genes ( GH3.3 , GH3.6 , IAA27 ) and leaf senescence genes ( SAG101 and SAG102 ). Contrarily, L-AOPP significantly reduced ABA, GA3 and JA content, down-regulated expression levels of GH3.1 , GH3.9 , IAA8 , bZIP11 , DREB2 , MYB14 , MYB48 , WRKY2 , WRKY56 , WRKY108715 , ERD and RD22 , and up-regulated expression levels of SAG101 and SAG102 . Collectively, these data suggested a positive role of exogenous IAA in alleviating drought damage through many involved physiological processes in white clover.

ACS Style

Youzhi Zhang; Yaping Li; Muhammad Jawad Hassan; Zhou Li; Yan Peng. Indole -3-acetic acid improves drought tolerance of white clover associated with activating auxin-related genes, abscisic acid and jasmonic acid-induced stress responsive transcription factors, and inhibiting senescence genes. 2020, 1 .

AMA Style

Youzhi Zhang, Yaping Li, Muhammad Jawad Hassan, Zhou Li, Yan Peng. Indole -3-acetic acid improves drought tolerance of white clover associated with activating auxin-related genes, abscisic acid and jasmonic acid-induced stress responsive transcription factors, and inhibiting senescence genes. . 2020; ():1.

Chicago/Turabian Style

Youzhi Zhang; Yaping Li; Muhammad Jawad Hassan; Zhou Li; Yan Peng. 2020. "Indole -3-acetic acid improves drought tolerance of white clover associated with activating auxin-related genes, abscisic acid and jasmonic acid-induced stress responsive transcription factors, and inhibiting senescence genes." , no. : 1.

Research article
Published: 09 January 2020 in Journal of Proteome Research
Reads 0
Downloads 0

The global emergence of soil salinization poses a serious challenge to many countries and regions. γ-Aminobutyric acid (GABA) is involved in systemic regulation of plant adaptation to salt stress but the underlying molecular and metabolic mechanism still remains largely unknown. The elevated endogenous GABA level by the application of exogenous GABA improved salt tolerance associated with the enhancement of antioxidant capacity, photosynthetic characteristics, osmotic adjustment (OA), and water use efficiency in creeping bentgrass. GABA strongly upregulated transcript levels of AsPPa2, AsATPaB2, AsNHX2/4/6, and AsSOS1/20 in roots involved in enhanced capacity of Na+ compartmentalization and mitigation of Na+ toxicity in the cytosol. Significant downregulation of AsHKT1/4 expression could be induced by GABA in leaves in relation to maintenance of the significantly lower Na+ content and higher K+/Na+ ratio. GABA-depressed aquaporin expression and accumulation induced declines in stomatal conductance and transpiration, thereby reducing water loss in leaves during salt stress. For metabolic regulation, GABA primarily enhanced sugar and amino acid accumulation and metabolism, largely contributing to improved salt tolerance through maintaining OA and metabolic homeostasis. Other major pathways could be related to GABA-induced salt tolerance including increases in antioxidant defense, heat shock proteins, and myo-inositol accumulation in leaves. Integrative analyses of molecular, protein, metabolic, and physiological changes reveal systemic functions of GABA in regulating ionic, water, and metabolic homeostasis in nonhalophytic creeping bentgrass under salt stress.

ACS Style

Zhou Li; Bizhen Cheng; Weihang Zeng; Xinquan Zhang; Yan Peng. Proteomic and Metabolomic Profilings Reveal Crucial Functions of γ-Aminobutyric Acid in Regulating Ionic, Water, and Metabolic Homeostasis in Creeping Bentgrass under Salt Stress. Journal of Proteome Research 2020, 19, 769 -780.

AMA Style

Zhou Li, Bizhen Cheng, Weihang Zeng, Xinquan Zhang, Yan Peng. Proteomic and Metabolomic Profilings Reveal Crucial Functions of γ-Aminobutyric Acid in Regulating Ionic, Water, and Metabolic Homeostasis in Creeping Bentgrass under Salt Stress. Journal of Proteome Research. 2020; 19 (2):769-780.

Chicago/Turabian Style

Zhou Li; Bizhen Cheng; Weihang Zeng; Xinquan Zhang; Yan Peng. 2020. "Proteomic and Metabolomic Profilings Reveal Crucial Functions of γ-Aminobutyric Acid in Regulating Ionic, Water, and Metabolic Homeostasis in Creeping Bentgrass under Salt Stress." Journal of Proteome Research 19, no. 2: 769-780.

Journal article
Published: 01 November 2019 in Plant Physiology and Biochemistry
Reads 0
Downloads 0

γ-Aminobutyric acid (GABA), a non-proteinaceous amino acid, modulates plant growth and stress tolerance. However, the potential role of GABA in regulating key metabolic pathways and stress-defensive proteins against drought in plants has never been explored. Creeping bentgrass (Agrostis stolonifera) plants were pretreated with or without GABA and then subjected to water stress for 8 days in controlled growth chambers (23/19 °C, day/night). Physiological analysis showed that elevated endogenous GABA level via exogenous GABA application significantly mitigated water stress damage to creeping bentgrass, as manifested by increased leaf relative water content, water use efficiency, osmotic adjustment (OA), photochemical efficiency (Fv/Fm), net photosynthetic rate, and reduced oxidative damage. iTRAQ-based proteomics found that enhanced chaperones accumulation, carbohydrates, amino acids, and energy metabolism played important roles in protein protection, OA, energy maintenance, and metabolic balance, which is important adaptive response to drought stress in creeping bentgrass. The GABA further promoted energy production and conversion, antioxidant defense, and DHN3 accumulation that were essential for energy requirement, ROS-scavenging, and the prevention of cell dehydration in leaf during drought stress. In addition, GABA-treated plants maintained significantly higher abundance of dicarboxylate transporter 2.1, ATP-dependent zinc metalloprotease, receptor-like protein kinase HERK1, o-acyltransferase WSD1, omega-6 fatty acid desaturase, and two-component response regulator ORR21 than untreated plants under drought stress. The result provides new evidences that GABA-induced drought tolerance is possibly involved in the improvement of nitrogen recycling, protection of photosystem II, mitigation of drought-depressed cell elongation, wax biosynthesis, fatty acid desaturase, and delaying leaf senescence in creeping bentgrass.

ACS Style

Zhou Li; Ting Huang; Mingyan Tang; Binzhen Cheng; Yan Peng; Xinquan Zhang. iTRAQ-based proteomics reveals key role of γ-aminobutyric acid (GABA) in regulating drought tolerance in perennial creeping bentgrass (Agrostis stolonifera). Plant Physiology and Biochemistry 2019, 145, 216 -226.

AMA Style

Zhou Li, Ting Huang, Mingyan Tang, Binzhen Cheng, Yan Peng, Xinquan Zhang. iTRAQ-based proteomics reveals key role of γ-aminobutyric acid (GABA) in regulating drought tolerance in perennial creeping bentgrass (Agrostis stolonifera). Plant Physiology and Biochemistry. 2019; 145 ():216-226.

Chicago/Turabian Style

Zhou Li; Ting Huang; Mingyan Tang; Binzhen Cheng; Yan Peng; Xinquan Zhang. 2019. "iTRAQ-based proteomics reveals key role of γ-aminobutyric acid (GABA) in regulating drought tolerance in perennial creeping bentgrass (Agrostis stolonifera)." Plant Physiology and Biochemistry 145, no. : 216-226.

Journal article
Published: 23 September 2019 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Activation and enhancement of heat shock factor (HSF) pathways are important adaptive responses to heat stress in plants. The γ-aminobutyric acid (GABA) plays an important role in regulating heat tolerance, but it is unclear whether GABA-induced thermotolerance is associated with activation of HSF pathways in plants. In this study, the changes of endogenous GABA level affecting physiological responses and genes involved in HSF pathways were investigated in creeping bentgrass during heat stress. The increase in endogenous GABA content induced by exogenous application of GABA effectively alleviated heat damage, as reflected by higher leaf relative water content, cell membrane stability, photosynthesis, and lower oxidative damage. Contrarily, the inhibition of GABA accumulation by the application of GABA biosynthesis inhibitor further aggravated heat damage. Transcriptional analyses showed that exogenous GABA could significantly upregulate transcript levels of genes encoding heat shock factor HSFs (HSFA-6a, HSFA-2c, and HSFB-2b), heat shock proteins (HSP17.8, HSP26.7, HSP70, and HSP90.1-b1), and ascorbate peroxidase 3 (APX3), whereas the inhibition of GABA biosynthesis depressed these genes expression under heat stress. Our results indicate GABA regulates thermotolerance associated with activation and enhancement of HSF pathways in creeping bentgrass.

ACS Style

Ting Liu; Zhaoqiao Liu; Zhou Li; Yan Peng; Xinquan Zhang; Xiao Ma; Linkai Huang; Wei Liu; Gang Nie; Liwen He. Regulation of Heat Shock Factor Pathways by γ-aminobutyric Acid (GABA) Associated with Thermotolerance of Creeping Bentgrass. International Journal of Molecular Sciences 2019, 20, 4713 .

AMA Style

Ting Liu, Zhaoqiao Liu, Zhou Li, Yan Peng, Xinquan Zhang, Xiao Ma, Linkai Huang, Wei Liu, Gang Nie, Liwen He. Regulation of Heat Shock Factor Pathways by γ-aminobutyric Acid (GABA) Associated with Thermotolerance of Creeping Bentgrass. International Journal of Molecular Sciences. 2019; 20 (19):4713.

Chicago/Turabian Style

Ting Liu; Zhaoqiao Liu; Zhou Li; Yan Peng; Xinquan Zhang; Xiao Ma; Linkai Huang; Wei Liu; Gang Nie; Liwen He. 2019. "Regulation of Heat Shock Factor Pathways by γ-aminobutyric Acid (GABA) Associated with Thermotolerance of Creeping Bentgrass." International Journal of Molecular Sciences 20, no. 19: 4713.

Original article
Published: 21 September 2019 in Planta
Reads 0
Downloads 0

β-sitosterol influences amino acids, carbohydrates, organic acids, and other metabolite metabolism and homeostasis largely contributing to better tolerance to water stress in white clover. β-sitosterol (BS) could act as an important plant growth regulator when plants are subjected to harsh environmental conditions. Objective of this study was to examine effects of BS on growth and water stress tolerance in white clover based on physiological responses and metabolomics. White clover was pretreated with or without BS and then subjected to water stress for 7 days in controlled growth chambers. Physiological analysis demonstrated that exogenous application of BS (120 μM) could significantly improve stress tolerance associated with better growth performance and photosynthesis, higher leaf relative water content, and less oxidative damage in white clover in response to water stress. Metabolic profiling identified 78 core metabolites involved in amino acids, organic acids, sugars, sugar alcohols, and other metabolites in leaves of white clover. For sugars and sugar alcohol metabolism, the BS treatment enhanced the accumulation of fructose, glucose, maltose, and myo-inositol contributing to better antioxidant capacity, growth maintenance, and osmotic adjustment in white clover under water stress. The application of BS was inclined to convert glutamic acid into proline, 5-oxoproline, and chlorophyll instead of going to pyruvate and alanine; the BS treatment did not significantly affect intermediates of tricarboxylic acid cycle (citrate, aconitate, and malate), but promoted the accumulation of other organic acids including lactic acid, glycolic acid, glyceric acid, shikimic acid, galacturonic acid, and quinic acid in white clover subjected to water stress. In addition, cysteine, an important antioxidant metabolite, was also significantly improved by BS in white clover under water stress. These altered amino acids and organic acids metabolism could play important roles in growth maintenance and modulation of osmotic and redox balance against water stress in white clover. Current findings provide a new insight into BS-induced metabolic homeostasis related to growth and water stress tolerance in plants.

ACS Style

Zhou Li; Bizhen Cheng; Bin Yong; Ting Liu; Yan Peng; Xinquan Zhang; Xiao Ma; Linkai Huang; Wei Liu; Gang Nie. Metabolomics and physiological analyses reveal β-sitosterol as an important plant growth regulator inducing tolerance to water stress in white clover. Planta 2019, 250, 2033 -2046.

AMA Style

Zhou Li, Bizhen Cheng, Bin Yong, Ting Liu, Yan Peng, Xinquan Zhang, Xiao Ma, Linkai Huang, Wei Liu, Gang Nie. Metabolomics and physiological analyses reveal β-sitosterol as an important plant growth regulator inducing tolerance to water stress in white clover. Planta. 2019; 250 (6):2033-2046.

Chicago/Turabian Style

Zhou Li; Bizhen Cheng; Bin Yong; Ting Liu; Yan Peng; Xinquan Zhang; Xiao Ma; Linkai Huang; Wei Liu; Gang Nie. 2019. "Metabolomics and physiological analyses reveal β-sitosterol as an important plant growth regulator inducing tolerance to water stress in white clover." Planta 250, no. 6: 2033-2046.

Journal article
Published: 28 March 2019 in Environmental and Experimental Botany
Reads 0
Downloads 0

Persistent high temperature inhibits plant growth and development. The γ-aminobutyric acid (GABA) plays a key role in heat tolerance in plants. The increase in endogenous GABA level induced by exogenous GABA effectively alleviated heat damage in creeping bentgrass. Global investigation into mRNAs and miRNAs found that the regulation of HSFs pathways and enhanced carbon metabolism, biosynthesis of amino acids, and plant hormone signal transduction were important adaptive response to heat stress. GABA-induced heat tolerance was closely related to phenylpropanoid biosynthesis, further improved HSFs pathways, and the upregulation of CAD3, ACS, AER, and CA1 associated with lignin and lipids biosynthesis, photosynthesis and water use, and antioxidant defense. Dramatic changes of miR398s, cca-miR156b, aly-miR159c-3p, and ata-miR408-3p have a close correlation with heat-survival. The especial vvi-miR845c, ama-miR156, and other novel miRNAs such as novel-24223, novel-2964, and novel-10098 could be involved in GABA-regulated heat tolerance. The integrated analysis of mRNAs and miRNAs facilitates uncovering of adaptative response in perennial plants under high temperature environmental condition and also reveals that GABA acting as a critical plant growth regulator induces heat tolerance in creeping bentgrass involved in physiological, transcriptional, and post-transcriptional regulation. Particularly, current results provide some key candidate genes and miRNAs for further investigating their potential roles in heat tolerance in non-model plants.

ACS Style

Zhou Li; Bizhen Cheng; Weihang Zeng; Zhaoqiao Liu; Yan Peng. The transcriptional and post-transcriptional regulation in perennial creeping bentgrass in response to γ-aminobutyric acid (GABA) and heat stress. Environmental and Experimental Botany 2019, 162, 515 -524.

AMA Style

Zhou Li, Bizhen Cheng, Weihang Zeng, Zhaoqiao Liu, Yan Peng. The transcriptional and post-transcriptional regulation in perennial creeping bentgrass in response to γ-aminobutyric acid (GABA) and heat stress. Environmental and Experimental Botany. 2019; 162 ():515-524.

Chicago/Turabian Style

Zhou Li; Bizhen Cheng; Weihang Zeng; Zhaoqiao Liu; Yan Peng. 2019. "The transcriptional and post-transcriptional regulation in perennial creeping bentgrass in response to γ-aminobutyric acid (GABA) and heat stress." Environmental and Experimental Botany 162, no. : 515-524.

Journal article
Published: 29 January 2019 in Hereditas
Reads 0
Downloads 0

Understanding genetic diversity of wild plant germplasm and the relationships between ecogeographic and genetic characteristics may provide insights for better utilizing and conserving genetic resources. Elymus tangutorum (Nevski) Hand.-Mazz, a cool-season hexaploid perennial, is an important pasture bunchgrass species used for forages and grassland restoration in Qinghai-Tibet Plateau and northwest China. In this study, 27 E. tangutorum accessions from diverse origins of western China were evaluated using AFLP markers in an effort to delve into the genetic relationships among them. The effects of eco-environmental factors and geographical isolation on the genetic diversity and population structure were also elucidated. On account of 554 polymorphic fragments amplified with 14 primer combinations, the mean values of some marker parameters including polymorphic information content, resolving power and marker index were 0.2504, 14.10 and 23.07, respectively, validating the high efficiency and reliability of the markers selected. Genetic dissimilarity index values among accessions ranged from 0.1024 to 0.7137 with a mean of 0.2773. STRUCTURE, UPGMA clustering and PCoA analyses showed that all accessions could be divided into the three main clusters; however, this results do not exactly coincide with geographic groups. We found medium differentiation (F ST = 0.162) between Qinghai-Tibet Plateau (QTP) and Xinjiang (XJC), and high differentiation (F ST = 0.188) among three Bayesian subgroups. A significant correlation (r = 0.312) between genetic and geographical distance was observed by Mantel test in the species level, while the weak correlation was detected between genetic and environmental distance for all accessions and most of geographical groups. In addition, a significant ecological influence of average annual precipitation on genetic distance was revealed in XJC group and the Bayesian subgroup A. This study indicates that AFLP technique are a useful tool to measure genetic diversity in E. tangutorum, showing that geographical and environmental factors (especially precipitation) together, play a crucial role in genetic differentiation patterns. These findings underline the importance of local adaptation in shaping patterns of genetic variability and population structure in E. tangutorum germplasm collected in Western China.

ACS Style

Wen-Dan Wu; Wen-Hui Liu; Ming Sun; Ji-Qiong Zhou; Wei Liu; Cheng-Lin Zhang; Xing-Quan Zhang; Yan Peng; Lin-Kai Huang; Xiao Ma. Genetic diversity and structure of Elymus tangutorum accessions from western China as unraveled by AFLP markers. Hereditas 2019, 156, 8 .

AMA Style

Wen-Dan Wu, Wen-Hui Liu, Ming Sun, Ji-Qiong Zhou, Wei Liu, Cheng-Lin Zhang, Xing-Quan Zhang, Yan Peng, Lin-Kai Huang, Xiao Ma. Genetic diversity and structure of Elymus tangutorum accessions from western China as unraveled by AFLP markers. Hereditas. 2019; 156 (1):8.

Chicago/Turabian Style

Wen-Dan Wu; Wen-Hui Liu; Ming Sun; Ji-Qiong Zhou; Wei Liu; Cheng-Lin Zhang; Xing-Quan Zhang; Yan Peng; Lin-Kai Huang; Xiao Ma. 2019. "Genetic diversity and structure of Elymus tangutorum accessions from western China as unraveled by AFLP markers." Hereditas 156, no. 1: 8.

Journal article
Published: 12 November 2018 in International Journal of Molecular Sciences
Reads 0
Downloads 0

This study was designed to examine the effects of NaCl pretreatment on the seed germination of white clover (Trifolium repens cv. Ladino) under water stress induced by 19% polyethylene glycol (PEG) 6000. Lower concentrations of NaCl (0.5, 1, and 2.5 mM) pretreatment significantly alleviated stress-induced decreases in germination percentage, germination vigor, germination index, and radicle length of seedlings after seven days of germination under water stress. The soaking with 1 mM of NaCl exhibited most the pronounced effects on improving seed germination and alleviating stress damage. NaCl-induced seeds germination and growth could be associated with the increases in endogenous gibberellic acid (GA) and indole-3-acetic acid (IAA) levels through activating amylases leading to improved amylolysis under water stress. Seedlings pretreated with NaCl had a significantly lower osmotic potential than untreated seedlings during seed germination, which could be related to significantly higher soluble sugars and free proline content in NaCl-treated seedlings under water stress. For antioxidant metabolism, NaCl pretreatment mainly improved superoxide dismutase, peroxidase, ascorbate peroxidase, and glutathione reductase activities, transcript levels of FeSOD, APX, and DHAR, and the content of ascorbic acid, reduced glutathione, and oxidized glutathione during seed germination under water stress. The results indicated that seeds soaking with NaCl could remarkably enhance antioxidant metabolism, thereby decreasing the accumulation of reactive oxygen species and membrane lipid peroxidation during germination under water stress. In addition, NaCl-upregulated dehydrin-encoded genes SK2 expression could be another important mechanism of drought tolerance during seeds germination of white clover in response to water stress.

ACS Style

Yiqin Cao; Linlin Liang; Bizhen Cheng; Yue Dong; Jiaqi Wei; Xiaolan Tian; Yan Peng; Zhou Li. Pretreatment with NaCl Promotes the Seed Germination of White Clover by Affecting Endogenous Phytohormones, Metabolic Regulation, and Dehydrin-Encoded Genes Expression under Water Stress. International Journal of Molecular Sciences 2018, 19, 3570 .

AMA Style

Yiqin Cao, Linlin Liang, Bizhen Cheng, Yue Dong, Jiaqi Wei, Xiaolan Tian, Yan Peng, Zhou Li. Pretreatment with NaCl Promotes the Seed Germination of White Clover by Affecting Endogenous Phytohormones, Metabolic Regulation, and Dehydrin-Encoded Genes Expression under Water Stress. International Journal of Molecular Sciences. 2018; 19 (11):3570.

Chicago/Turabian Style

Yiqin Cao; Linlin Liang; Bizhen Cheng; Yue Dong; Jiaqi Wei; Xiaolan Tian; Yan Peng; Zhou Li. 2018. "Pretreatment with NaCl Promotes the Seed Germination of White Clover by Affecting Endogenous Phytohormones, Metabolic Regulation, and Dehydrin-Encoded Genes Expression under Water Stress." International Journal of Molecular Sciences 19, no. 11: 3570.

Journal article
Published: 25 August 2018 in International Journal of Molecular Sciences
Reads 0
Downloads 0

The objective of this study was to determine the effect of soaking with γ-aminobutyric acid (GABA) on white clover (Trifolium repens cv. Haifa) seed germination under salt stress induced by 100 mM NaCl. Seeds soaking with GABA (1 μM) significantly alleviated salt-induced decreases in endogenous GABA content, germination percentage, germination vigor, germination index, shoot and root length, fresh and dry weight, and root activity of seedling during seven days of germination. Exogenous application of GABA accelerated starch catabolism via the activation of amylase and also significantly reduced water-soluble carbohydrate, free amino acid, and free proline content in seedlings under salt stress. In addition, improved antioxidant enzyme activities (SOD, GPOX, CAT, APX, DHAR, GR and MDHR) and gene transcript levels (Cu/ZnSOD, FeSOD, MnSOD, CAT, GPOX, APX, MDHR, GPX and GST) was induced by seeds soaking with GABA, followed by decreases in O2∙−, H2O2, and MDA accumulation during germination under salt stress. Seeds soaking with GABA could also significantly improve Na+/K+ content and transcript levels of genes encoding Na+/K+ transportation (HKT1, HKT8, HAL2, H+-ATPase and SOS1) in seedlings of white clover. Moreover, exogenous GABA significantly induced the accumulation of dehydrins and expression of genes encoding dehydrins (SK2, Y2K, Y2SK, and dehydrin b) in seedlings under salt stress. These results indicate that GABA mitigates the salt damage during seeds germination through enhancing starch catabolism and the utilization of sugar and amino acids for the maintenance of growth, improving the antioxidant defense for the alleviation of oxidative damage, increasing Na+/K+ transportation for the osmotic adjustment, and promoting dehydrins accumulation for antioxidant and osmotic adjustment under salt stress.

ACS Style

Bizhen Cheng; Zhou Li; Linlin Liang; Yiqin Cao; Weihang Zeng; Xinquan Zhang; Xiao Ma; Linkai Huang; Gang Nie; Wei Liu; Yan Peng. The γ-Aminobutyric Acid (GABA) Alleviates Salt Stress Damage during Seeds Germination of White Clover Associated with Na+/K+ Transportation, Dehydrins Accumulation, and Stress-Related Genes Expression in White Clover. International Journal of Molecular Sciences 2018, 19, 2520 .

AMA Style

Bizhen Cheng, Zhou Li, Linlin Liang, Yiqin Cao, Weihang Zeng, Xinquan Zhang, Xiao Ma, Linkai Huang, Gang Nie, Wei Liu, Yan Peng. The γ-Aminobutyric Acid (GABA) Alleviates Salt Stress Damage during Seeds Germination of White Clover Associated with Na+/K+ Transportation, Dehydrins Accumulation, and Stress-Related Genes Expression in White Clover. International Journal of Molecular Sciences. 2018; 19 (9):2520.

Chicago/Turabian Style

Bizhen Cheng; Zhou Li; Linlin Liang; Yiqin Cao; Weihang Zeng; Xinquan Zhang; Xiao Ma; Linkai Huang; Gang Nie; Wei Liu; Yan Peng. 2018. "The γ-Aminobutyric Acid (GABA) Alleviates Salt Stress Damage during Seeds Germination of White Clover Associated with Na+/K+ Transportation, Dehydrins Accumulation, and Stress-Related Genes Expression in White Clover." International Journal of Molecular Sciences 19, no. 9: 2520.

Journal article
Published: 01 August 2018 in Plant Physiology and Biochemistry
Reads 0
Downloads 0

Endogenous hormones and polyamines (PAs) could interact to regulate growth and tolerance to water stress in white clover. The objective of this study was to investigate whether the alteration of endogenous indole-3-acetic acid (IAA) level affected other hormones level and PAs metabolism contributing to the regulation of tolerance to water stress in white clover. Plants were pretreated with IAA or L-2-aminooxy-3-phenylpropionic acid (L-AOPP, the inhibitor of IAA biosynthesis) for 3 days and then subjected to water-sufficient condition and water stress induced by 15% polyethylene glycol 6000 for 8 days in growth chambers. Exogenous application of IAA significantly increased endogenous IAA, gibberellin (GA), abscisic acid (ABA), and polyamine (PAs) levels, but had no effect on cytokinin content under water stress. The increase in endogenous IAA level enhanced PAs anabolism via the improvement of enzyme activities and transcript level of genes including arginine decarboxylase, ornithine decarboxylase, and S-adenosylmethionine decarboxylase. Exogenous application of IAA also affected PAs catabolism, as manifested by an increase in diamine oxidase and a decrease in polyamine oxidase activities and genes expression. More importantly, the IAA deficiency in white clover decreased endogenous hormone levels (GA, ABA, and PAs) and PAs anabolism along with decline in antioxidant defense and osmotic adjustment (OA). On the contrary, exogenous IAA effectively alleviated stress-induced oxidative damage, growth inhibition, water deficit, and leaf senescence through the maintenance of higher chlorophyll content, OA, and antioxidant defense as well as lower transcript levels of senescence marker genes SAG101 and SAG102 in leaves under water stress. These results indicate that IAA-induced the crosstalk between endogenous hormones and PAs could be involved in the improvement of antioxidant defense and OA conferring tolerance to water stress in white clover.

ACS Style

Zhou Li; Yaping Li; Yan Zhang; Bizhen Cheng; Yan Peng; Xinquan Zhang; Xiao Ma; Linkai Huang; Yanhong Yan. Indole-3-acetic acid modulates phytohormones and polyamines metabolism associated with the tolerance to water stress in white clover. Plant Physiology and Biochemistry 2018, 129, 251 -263.

AMA Style

Zhou Li, Yaping Li, Yan Zhang, Bizhen Cheng, Yan Peng, Xinquan Zhang, Xiao Ma, Linkai Huang, Yanhong Yan. Indole-3-acetic acid modulates phytohormones and polyamines metabolism associated with the tolerance to water stress in white clover. Plant Physiology and Biochemistry. 2018; 129 ():251-263.

Chicago/Turabian Style

Zhou Li; Yaping Li; Yan Zhang; Bizhen Cheng; Yan Peng; Xinquan Zhang; Xiao Ma; Linkai Huang; Yanhong Yan. 2018. "Indole-3-acetic acid modulates phytohormones and polyamines metabolism associated with the tolerance to water stress in white clover." Plant Physiology and Biochemistry 129, no. : 251-263.