Hedychium coronarium J. Koening, belonging to Zingiberaceae family, is a perennial herb with fleshly aromatic rhizomes. There are no information about the antiplatelet properties of essential oils (EOs) from rhizomes (HCR) and leaves (HCL) of this herb, additionally, there are reports about the antibacterial activity of the Zingiberaceae species, however, no studies have been carried out in the Colombian Amazon Region. The EOs were characterized by GC-MS, the antiaggregant activity was assessed by ADP and Collagen as platelet agonist and the antibacterial activity against E. faecalis and S. aureus were evidenced by the determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). A high content of oxygenated monoterpenes were found in HCL essential oil (EO) and 20 compounds were identified in HCR EO. The HCL EO showed antiaggregant activity when collagen was used and HCR EO showed a concentration-dependent activity against ADP and collagen, meanwhile only the HCR EO showed antibacterial activity against E. faecalis and S. aureus.

Thrombotic cardiovascular diseases are frequent side effects of cancer therapy with cytotoxic drugs such as Doxorubicin. Endothelial cell senescence is emerging as a critical mechanism underlying endothelial dysfunction in this context. Senescent cells, although unable to proliferate, secrete bioactive molecules that alter the tissue microenvironment, a feature known as the senescence-associated secretory phenotype (SASP). Besides soluble molecules, senescent cells also release extracellular vesicles (EVs). Previous studies indicate that senescent endothelial cells produce a secretome that promotes platelet activation; however, the contribution of EVs remains unclear. Here, we show that human microvascular endothelial cells (HMEC-1) exposed to Doxorubicin undergo senescence, display endothelial dysfunction, and release EVs. We found no differences in the concentration or size distribution of EVs from senescent and non-senescent cells. Nevertheless, EVs from senescent HMEC-1 promoted platelet activation more strongly than EVs from control cells. Notably, EVs alone did not induce platelet aggregation, suggesting that soluble factors are also required to support platelet-dependent hemostasis. These findings reveal that EVs from senescent endothelial cells contribute to platelet activation, a process that may favor thrombosis in patients receiving Doxorubicin-based chemotherapy.

The rapid emergence of antimicrobial resistance has intensified the search for alternative antimicrobial scaffolds that target both planktonic bacteria and biofilm-associated infections. In this study, a series of hydroxylated phosphonium ionic liquids derived from triphenylphosphonium (TPP⁺) and trihexylphosphonium (THP⁺) cations bearing C3, C6, C7, and C10 ω-hydroxyalkyl chains were synthesized and evaluated for their antibacterial and anti-biofilm activities. Antibacterial activity was determined using broth microdilution assays against Staphylococcus aureus and Escherichia coli, while anti-biofilm activity was assessed by disrupting preformed biofilms using a 96-pin microtiter plate system and crystal violet staining. The results showed that antibacterial activity was strongly influenced by the amphiphilic balance of the compounds, particularly the alkyl chain length and the nature of the phosphonium core. Derivatives bearing C6OH–C10OH chains exhibited the highest antibacterial activity, whereas short-chain analogs displayed markedly reduced potency. THP derivatives were notably more active against E. coli bacteria, consistent with their higher hydrophobicity and activity consistent with membrane interaction. In addition, THP derivatives demonstrated greater biofilm disruption, achieving up to ~90% biomass removal in E. coli biofilms, with C6OH–C7OH derivatives showing the most favorable activity profile. Hemolysis assays indicated low erythrocyte toxicity at concentrations close to antibacterial MIC values, indicating a favorable selectivity window. Overall, these findings highlight phosphonium ionic liquids as promising antimicrobial agents with activity consistent with membrane interaction and provide structure–activity insights for the rational design of new antibacterial and anti-biofilm compounds.