This collaborative work by over 180 researchers from 40+ countries addresses the challenges posed by “phantom agents”—putative pathogenic agents named in literature without supporting data on their existence. Those agents remain on regulatory lists, creating barriers in trade and plant certification. Historically identified based solely on symptoms, these agents lack isolates or sequence data, making reliable detection or risk assessment impossible. After reviewing over 120 such agents across 10 key plant genera, we recommend their removal from regulatory lists and call for revised standards aligned with modern diagnostics. This effort seeks to streamline germplasm exchange, benefiting global agriculture by removing the constraints imposed by phantoms.

Taro (Colocasia esculenta) is a socioeconomically and nutritionally important crop that is predominantly cultivated in the derived savanna and humid forest agroecosystems of Nigeria. Taro production in the country has declined since the taro leaf blight (TLB) outbreak caused by Phytophthora colocasiae Raciborski. This study conducted field surveys during the 2021–2022 production season to assess the status of taro diseases, as well as a structured questionnaire to capture farmers’ management practices and the socio-economic determinants of taro cultivation across seven major taro-producing states in Nigeria. Data was collected from 63 randomly selected farmers across 53 farms, and 449 corms were sampled from farms and markets to assess corm-borne diseases. Sixty-three percent of farmers identified biotic constraints as the major production challenge, with TLB recognized as the most significant threat. Virus-symptomatic plants were not observed in the farmers’ fields, but the occurrence of Dasheen mosaic virus (or Potyvirus dasheenis) (DsMV, genus Potyvirus) was detected among the plants regenerated from corms collected from farms and markets. The widespread occurrence of TLB and DsMV suggests that these two pathogens pose a serious threat to taro production and that there is a risk of further spread through the continuous recycling of self-sourced planting materials across seasons.

RNA-dependent RNA polymerase, or RdRp, remains the central molecular hallmark of RNA viruses. It serves as both a universal anchor for virus detection and a critical target for understanding the functional and evolutionary properties of RNA viruses. Since the inaugural RdRp summit in 2023, there have been significant advances in sequencing, structural prediction and artificial intelligence, all of which have accelerated the pace of RNA virus discovery and taxonomic annotation, revealing unprecedented levels of viral diversity, including novel phyla and unique genome architectures. Recent advances include the discovery of novel viral phyla such as Ambiviricota and the application of AI-driven models like LucaProt, highlighting both the rapid expansion of viral diversity and the growing role of machine learning in RNA virus research. The second RdRp summit, which was held in Lisbon in May 2025, gathered a group of research scientists from diverse subfields of virology to address emerging challenges in RNA virus biology. These challenges ranged from standardising annotation and data sharing to harnessing structure-guided phylogenetics and petabyte-scale computational tools. Here, our consensus statement outlines key progress, current and future challenges and community-driven initiatives, including benchmarking, virus-host inference, and ongoing knowledge exchange efforts - all of which are designed to unify the field. Importantly, this statement reflects a clear community consensus and provides concrete recommendations to prioritize standardized benchmarking, structure-informed evolutionary analysis, and reproducible virus–host inference as foundational pillars for advancing RNA virus research. By fostering an environment of sustained collaboration, our efforts aim to build a coherent framework for modern RNA virus biology and to accelerate the exploration of the hidden RNA virosphere.

Yams ( Dioscorea spp.) are an important vegetatively propagated food security crop grown for their starchy tubers. Yams are susceptible to several viruses, and their genomes harbor a diverse array of endogenous pararetroviral sequences (EPRVs), which complicate diagnostics and germplasm exchange because of their similarity to episomal viruses. To better characterize EPRV diversity, we analyzed 86 publicly available whole-genome sequences from five Dioscorea species ( D. rotundata , D. alata , D. praehensilis , D. abyssinica , and D. dumetorum ). Assembled genomes were screened for endogenous pararetrovirus sequences (EPRVs) using the CAULIFINDER pipeline, targeting conserved RT/RNase H domains of the Caulimoviridae family. Our analyses revealed four major EPRV groups in D. rotundata : Yendovirus, Badnavirus, Yam Endovirus 1, and a Dioscovirus-like lineage. While most insertions were fragmented, we found full-length putative viral genomes corresponding to the Yam Badnavirus and Dioscovirus-like clades. These findings expand knowledge of yam EPRV diversity, contribute to the development of improved diagnostic tools to differentiate endogenous and episomal forms, and promote a science-based, risk-proportionate approach to yam phytosanitation that facilitates global germplasm exchange while maintaining biosecurity.