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Within the carboxylate platform, carboxylic acids are seen as important building-blocks for the chemical industry, but their recovery is challenging. Membrane based reactive extraction (MBRE) was evaluated for the recovery of these acids from a fermentor in which a mixed culture is converting biorefinery wastewater to carboxylates. Both off-line and in-line experiments were performed. The off-line experiments were promising and extraction efficiencies of 15 (acetic acid) to almost 100 % (caproic acid) were reached. The in-line experiments confirmed the selective and near complete extraction of carboxylates with longer chain length. However the overall extraction rates were lower than in off-line tests and the total carboxylate production rate was also decreasing. This was probably caused by limited but repeating contact between organic phase and fermentation broth via the membrane interface.
Kristien De Sitter; Linsey Garcia-Gonzalez; Claudia Matassa; Lorenzo Bertin; Heleen De Wever. The use of membrane based reactive extraction for the recovery of carboxylic acids from thin stillage. Separation and Purification Technology 2018, 206, 177 -185.
AMA StyleKristien De Sitter, Linsey Garcia-Gonzalez, Claudia Matassa, Lorenzo Bertin, Heleen De Wever. The use of membrane based reactive extraction for the recovery of carboxylic acids from thin stillage. Separation and Purification Technology. 2018; 206 ():177-185.
Chicago/Turabian StyleKristien De Sitter; Linsey Garcia-Gonzalez; Claudia Matassa; Lorenzo Bertin; Heleen De Wever. 2018. "The use of membrane based reactive extraction for the recovery of carboxylic acids from thin stillage." Separation and Purification Technology 206, no. : 177-185.
White biotechnology is promising to transform CO2 emissions into a valuable commodity chemical such as the biopolymer polyhydroxyalkanaotes (PHA). Our calculations indicated that the indirect conversion of acetic acid from CO2 into PHA is an interesting alternative for the direct production of PHA from CO2 in terms of CO2 fixation, H2 consumption, substrate cost, safety and process performance. An alternative cultivation method using acetic acid as an indirect sink of CO2 was therefore developed and a proof-of-concept provided for the synthesis of both the homopolymer poly(3-hydroxybutyrate) (PHB) and the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). The aim was to compare key performance parameters with those of existing cultivation methods for direct conversion of CO2 to PHA. Fed-batch cultivations for PHA production were performed using a pH-stat fed-batch feeding strategy in combination with an additional Dissolved Oxygen (DO)-dependent feed. After 118 h of fermentation, 60 g/L cell dry matter (CDM) containing 72% of PHB was obtained, which are the highest result values reported so far. Fed-batch cultivations for PHBV production resulted in 65 g/L CDM and 48 g/L PHBV concentration with a 3HV fraction of 27 mol %. Further research should be oriented towards process optimisation, whole process integration and design, and techno-economic assessment.
Linsey Garcia-Gonzalez; Heleen De Wever. Acetic Acid as an Indirect Sink of CO2 for the Synthesis of Polyhydroxyalkanoates (PHA): Comparison with PHA Production Processes Directly Using CO2 as Feedstock. Applied Sciences 2018, 8, 1416 .
AMA StyleLinsey Garcia-Gonzalez, Heleen De Wever. Acetic Acid as an Indirect Sink of CO2 for the Synthesis of Polyhydroxyalkanoates (PHA): Comparison with PHA Production Processes Directly Using CO2 as Feedstock. Applied Sciences. 2018; 8 (9):1416.
Chicago/Turabian StyleLinsey Garcia-Gonzalez; Heleen De Wever. 2018. "Acetic Acid as an Indirect Sink of CO2 for the Synthesis of Polyhydroxyalkanoates (PHA): Comparison with PHA Production Processes Directly Using CO2 as Feedstock." Applied Sciences 8, no. 9: 1416.
As one of the key enabling technologies, industrial biotechnology has a high potential to tackle harmful CO2 emissions and to turn CO2 into a valuable commodity. So far, experimental work mainly focused on the bioconversion of pure CO2 to chemicals and plastics and little is known about the tolerance of the bioprocesses to the presence of impurities. This work is the first to investigate the impact of real CO2-rich off-gases on autotrophic production of polyhydroxybutyrate. To this end, two-phase heterotrophic-autotrophic fermentation experiments were set up, consisting of heterothrophic cell mass growth using glucose as substrate followed by autotrophic biopolymer production using either pure synthetic CO2 or industrial off-gases sampled at two point sources. The use of real off-gases did not affect the bacterial performance. High biopolymer content (up to 73%) and productivities (up to 0.227 g/lh) were obtained. Characterisation of the polymers showed that all biopolymers had similar properties, independent of the CO2 source. Moreover, the CO2-derived biopolymers’ properties were comparable to commercial ones and biopolymers reported in literature, which are all produced from organic carbon sources.
Linsey Garcia-Gonzalez; Heleen De Wever. Valorisation of CO2-rich off-gases to biopolymers through biotechnological process. FEMS Microbiology Letters 2017, 364, 1 .
AMA StyleLinsey Garcia-Gonzalez, Heleen De Wever. Valorisation of CO2-rich off-gases to biopolymers through biotechnological process. FEMS Microbiology Letters. 2017; 364 (20):1.
Chicago/Turabian StyleLinsey Garcia-Gonzalez; Heleen De Wever. 2017. "Valorisation of CO2-rich off-gases to biopolymers through biotechnological process." FEMS Microbiology Letters 364, no. 20: 1.
Salatul Islam Mozumder; Linsey Garcia-Gonzalez; Heleen De Wever; Eveline I.P. Volcke. Model-based process analysis of heterotrophic-autotrophic poly(3-hydroxybutyrate) (PHB) production. Biochemical Engineering Journal 2016, 114, 202 -208.
AMA StyleSalatul Islam Mozumder, Linsey Garcia-Gonzalez, Heleen De Wever, Eveline I.P. Volcke. Model-based process analysis of heterotrophic-autotrophic poly(3-hydroxybutyrate) (PHB) production. Biochemical Engineering Journal. 2016; 114 ():202-208.
Chicago/Turabian StyleSalatul Islam Mozumder; Linsey Garcia-Gonzalez; Heleen De Wever; Eveline I.P. Volcke. 2016. "Model-based process analysis of heterotrophic-autotrophic poly(3-hydroxybutyrate) (PHB) production." Biochemical Engineering Journal 114, no. : 202-208.
Linsey Garcia-Gonzalez; Salatul Islam Mozumder; Majorie Dubreuil; Eveline I.P. Volcke; Heleen De Wever. Sustainable autotrophic production of polyhydroxybutyrate (PHB) from CO 2 using a two-stage cultivation system. Catalysis Today 2015, 257, 237 -245.
AMA StyleLinsey Garcia-Gonzalez, Salatul Islam Mozumder, Majorie Dubreuil, Eveline I.P. Volcke, Heleen De Wever. Sustainable autotrophic production of polyhydroxybutyrate (PHB) from CO 2 using a two-stage cultivation system. Catalysis Today. 2015; 257 ():237-245.
Chicago/Turabian StyleLinsey Garcia-Gonzalez; Salatul Islam Mozumder; Majorie Dubreuil; Eveline I.P. Volcke; Heleen De Wever. 2015. "Sustainable autotrophic production of polyhydroxybutyrate (PHB) from CO 2 using a two-stage cultivation system." Catalysis Today 257, no. : 237-245.
Linsey Garcia-Gonzalez; Sebastiaan Bijttebier; Winnie Dejonghe; Karolien Vanbroekhoven; Heleen De Wever; Stefan Voorspoels; Maarten Uyttebroek; Kathy Elst; Yamini Satyawali; Deepak Pant. Cascaded Valorization of Food Waste using Bioconversions as Core Processes. Advances in Food Biotechnology 2015, 427 -442.
AMA StyleLinsey Garcia-Gonzalez, Sebastiaan Bijttebier, Winnie Dejonghe, Karolien Vanbroekhoven, Heleen De Wever, Stefan Voorspoels, Maarten Uyttebroek, Kathy Elst, Yamini Satyawali, Deepak Pant. Cascaded Valorization of Food Waste using Bioconversions as Core Processes. Advances in Food Biotechnology. 2015; ():427-442.
Chicago/Turabian StyleLinsey Garcia-Gonzalez; Sebastiaan Bijttebier; Winnie Dejonghe; Karolien Vanbroekhoven; Heleen De Wever; Stefan Voorspoels; Maarten Uyttebroek; Kathy Elst; Yamini Satyawali; Deepak Pant. 2015. "Cascaded Valorization of Food Waste using Bioconversions as Core Processes." Advances in Food Biotechnology , no. : 427-442.
Salatul Islam Mozumder; Linsey Garcia-Gonzalez; Heleen De Wever; Eveline I.P. Volcke. Poly(3-hydroxybutyrate) (PHB) production from CO2: Model development and process optimization. Biochemical Engineering Journal 2015, 98, 107 -116.
AMA StyleSalatul Islam Mozumder, Linsey Garcia-Gonzalez, Heleen De Wever, Eveline I.P. Volcke. Poly(3-hydroxybutyrate) (PHB) production from CO2: Model development and process optimization. Biochemical Engineering Journal. 2015; 98 ():107-116.
Chicago/Turabian StyleSalatul Islam Mozumder; Linsey Garcia-Gonzalez; Heleen De Wever; Eveline I.P. Volcke. 2015. "Poly(3-hydroxybutyrate) (PHB) production from CO2: Model development and process optimization." Biochemical Engineering Journal 98, no. : 107-116.
Microalgae are a potential source for various valuable chemicals for commercial applications ranging from nutraceuticals to fuels. Objective in a biorefinery is to utilize biomass ingredients efficiently similarly to petroleum refineries in which oil is fractionated in fuels and a variety of products with higher value. Downstream processes in microalgae biorefineries consist of different steps whereof cell disruption is the most crucial part. To maintain the functionality of algae biochemicals during cell disruption while obtaining high disruption yields is an important challenge. Despite this need, studies on mild disruption of microalgae cells are limited. This review article focuses on the evaluation of conventional and emerging cell disruption technologies, and a comparison thereof with respect to their potential for the future microalgae biorefineries. The discussed techniques are bead milling, high pressure homogenization, high speed homogenization, ultrasonication, microwave treatment, pulsed electric field treatment, non-mechanical cell disruption and some emerging technologies.
E. Günerken; E. D'Hondt; M.H.M. Eppink; L. Garcia-Gonzalez; K. Elst; R.H. Wijffels. Cell disruption for microalgae biorefineries. Biotechnology Advances 2015, 33, 243 -260.
AMA StyleE. Günerken, E. D'Hondt, M.H.M. Eppink, L. Garcia-Gonzalez, K. Elst, R.H. Wijffels. Cell disruption for microalgae biorefineries. Biotechnology Advances. 2015; 33 (2):243-260.
Chicago/Turabian StyleE. Günerken; E. D'Hondt; M.H.M. Eppink; L. Garcia-Gonzalez; K. Elst; R.H. Wijffels. 2015. "Cell disruption for microalgae biorefineries." Biotechnology Advances 33, no. 2: 243-260.
Salatul Islam Mozumder; Heleen De Wever; Eveline I.P. Volcke; Linsey Garcia-Gonzalez. A robust fed-batch feeding strategy independent of the carbon source for optimal polyhydroxybutyrate production. Process Biochemistry 2014, 49, 365 -373.
AMA StyleSalatul Islam Mozumder, Heleen De Wever, Eveline I.P. Volcke, Linsey Garcia-Gonzalez. A robust fed-batch feeding strategy independent of the carbon source for optimal polyhydroxybutyrate production. Process Biochemistry. 2014; 49 (3):365-373.
Chicago/Turabian StyleSalatul Islam Mozumder; Heleen De Wever; Eveline I.P. Volcke; Linsey Garcia-Gonzalez. 2014. "A robust fed-batch feeding strategy independent of the carbon source for optimal polyhydroxybutyrate production." Process Biochemistry 49, no. 3: 365-373.
In this contribution a mechanistic model describing the production of polyhydroxybutyrate (PHB) through pure-culture fermentation was developed, calibrated and validated for two different substrates, namely glucose and waste glycerol. In both cases, non-growth-associated PHB production was triggered by applying nitrogen limitation. The occurrence of some growth-associated PHB production besides non-growth-associated PHB production was demonstrated, although it is inhibited in the presence of nitrogen. Other phenomena observed experimentally and described by the model included biomass growth on PHB and non-linear product inhibition of PHB production. The accumulated impurities from the waste substrate negatively affected the obtained maximum PHB content. Overall, the developed mathematical model provided an accurate prediction of the dynamic behavior of heterotrophic biomass growth and PHB production in a two-phase pure culture system.
Salatul Islam Mozumder; Laurens Goormachtigh; Linsey Garcia-Gonzalez; Heleen De Wever; Eveline Volcke. Modeling pure culture heterotrophic production of polyhydroxybutyrate (PHB). Bioresource Technology 2014, 155, 272 -280.
AMA StyleSalatul Islam Mozumder, Laurens Goormachtigh, Linsey Garcia-Gonzalez, Heleen De Wever, Eveline Volcke. Modeling pure culture heterotrophic production of polyhydroxybutyrate (PHB). Bioresource Technology. 2014; 155 ():272-280.
Chicago/Turabian StyleSalatul Islam Mozumder; Laurens Goormachtigh; Linsey Garcia-Gonzalez; Heleen De Wever; Eveline Volcke. 2014. "Modeling pure culture heterotrophic production of polyhydroxybutyrate (PHB)." Bioresource Technology 155, no. : 272-280.