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Dr. Maria João Ramalhosa
REQUIMTE–LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Porto, Portugal

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0 Analytical Chemistry
0 Environmental Chemistry
0 Food Chemistry
0 Food Science
0 Green Chemistry

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Journal article
Published: 12 June 2021 in Foods
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The total lipid content and lipidic profile of seaweeds harvested in the North Coast and purchased in Portugal were determined in this paper. The amount of total lipids in the different species of seaweeds varied between 0.7 ± 0.1% (Chondrus crispus) and 3.8 ± 0.6% (Ulva spp.). Regarding the fatty acid content, polyunsaturated fatty acids (PUFA) ranged between 0–35%, with Ulva spp. presenting the highest amount; monounsaturated fatty acids (MUFA) varied between 19 and 67%; and saturated fatty acids (SFA) were predominant in C. crispus (45–78%) and Gracilaria spp. (36–79%). Concerning the nutritional indices, the atherogenicity index (AI) was between 0.4–3.2, the thrombogenicity index (TI) ranged from 0.04 to 1.95, except for Gracilaria spp., which had a TI of 7.6, and the hypocholesterolemic/hypercholesterolemic ratio (HH) values ranged between 0.88–4.21, except for Gracilaria spp., which exhibited values between 0.22–9.26. The n6/n3 ratio was below 1 for most of the species evaluated, except for Ascophyllum nodosum, which presented a higher value, although below 2. Considering the PUFA/SFA ratio, seaweeds presented values between 0.11–1.02. The polycyclic aromatic hydrocarbons (PAHs) and aliphatic hydrocarbons (AHCs) contamination of seaweeds under study was also quantified, the values found being much lower than the maximum levels recommended for foodstuff.

ACS Style

Cristina Soares; Sara Sousa; Susana Machado; Elsa Vieira; Ana Carvalho; Maria Ramalhosa; Simone Morais; Manuela Correia; Teresa Oliva-Teles; Valentina Domingues; Cristina Delerue-Matos. Bioactive Lipids of Seaweeds from the Portuguese North Coast: Health Benefits versus Potential Contamination. Foods 2021, 10, 1366 .

AMA Style

Cristina Soares, Sara Sousa, Susana Machado, Elsa Vieira, Ana Carvalho, Maria Ramalhosa, Simone Morais, Manuela Correia, Teresa Oliva-Teles, Valentina Domingues, Cristina Delerue-Matos. Bioactive Lipids of Seaweeds from the Portuguese North Coast: Health Benefits versus Potential Contamination. Foods. 2021; 10 (6):1366.

Chicago/Turabian Style

Cristina Soares; Sara Sousa; Susana Machado; Elsa Vieira; Ana Carvalho; Maria Ramalhosa; Simone Morais; Manuela Correia; Teresa Oliva-Teles; Valentina Domingues; Cristina Delerue-Matos. 2021. "Bioactive Lipids of Seaweeds from the Portuguese North Coast: Health Benefits versus Potential Contamination." Foods 10, no. 6: 1366.

Original paper
Published: 24 May 2021 in Waste and Biomass Valorization
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Recently, the value of wood residues has received increasing attention due to the presence of pharmacologically active compounds. In the present study, apple bark was treated with subcritical water (SWE) with the aim of bioactivity determination and chemical analysis by GC–MS applying two different sample preparation protocols and by HPLC. Extracts were screened for their total antioxidant capacity by means of total phenolic (TPC) and flavonoid contents (TFC), ferric reducing antioxidant power (FRAP) and DPPH-radical scavenging activity (DPPH-RSA). Anti-microbial and anti-proliferative activities of the apple bark subcritical water extracts were also examined. Different lignin and cellulose degradation products were identified by GC–MS, as well as naturally present compounds. Identified compounds encompassed organic and fatty acids, alcohols, sugars, phenol, benzaldehyde, cinnamic acid, cinnamaldehyde and azulene derivatives, as well as other organic compounds. Phenolic profiles of apple bark extracts defined by HPLC–PDA analysis revealed that the main contributors were gallic acid (9.4 ± 0.5 mg/g DW) and catechin (8.3 ± 0.4 mg/g DW). The diversity of the extracts composition supports the fact that this medium is able to extract compounds of different polarities, simultaneously acting as catalyst and reactant. The characterized apple bark extracts demonstrated good antioxidant (TPC = 31.47 ± 1.86 mg GAE/g; TFC = 17.40 ± 1.89 mg EE/g; FRAP = 22.45 ± 2.22 mg AAE/g; DPPH-RSA = 22.57 ± 2.24 mg Trolox E/g DW), antimicrobial (MIC = 19.53–156.25 μg/mL) and anti-proliferative activity (IC50 = 19.88–47.44 μg/mL). The findings of this study showed that SWE can be used as an environmentally friendly and safe technique for the valorization of apple tree wood residues, applicable in food and pharmaceutical industries.

ACS Style

Jaroslava Švarc-Gajić; Víctor Cerdà; Cristina Delerue-Matos; Pavle Mašković; Sabrina Clavijo; Ruth Suarez; Aleksandra Cvetanović; Maria João Ramalhosa; M. Fátima Barroso; Manuela Moreira; Simone Morais; Hannes Withouck; Annick Boeykens. Chemical Characterization and In Vitro Bioactivity of Apple Bark Extracts Obtained by Subcritical Water. Waste and Biomass Valorization 2021, 1 -14.

AMA Style

Jaroslava Švarc-Gajić, Víctor Cerdà, Cristina Delerue-Matos, Pavle Mašković, Sabrina Clavijo, Ruth Suarez, Aleksandra Cvetanović, Maria João Ramalhosa, M. Fátima Barroso, Manuela Moreira, Simone Morais, Hannes Withouck, Annick Boeykens. Chemical Characterization and In Vitro Bioactivity of Apple Bark Extracts Obtained by Subcritical Water. Waste and Biomass Valorization. 2021; ():1-14.

Chicago/Turabian Style

Jaroslava Švarc-Gajić; Víctor Cerdà; Cristina Delerue-Matos; Pavle Mašković; Sabrina Clavijo; Ruth Suarez; Aleksandra Cvetanović; Maria João Ramalhosa; M. Fátima Barroso; Manuela Moreira; Simone Morais; Hannes Withouck; Annick Boeykens. 2021. "Chemical Characterization and In Vitro Bioactivity of Apple Bark Extracts Obtained by Subcritical Water." Waste and Biomass Valorization , no. : 1-14.

Journal article
Published: 01 December 2020 in Food and Chemical Toxicology
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This work assessed the concentrations of 18 polycyclic aromatic hydrocarbons (PAHs) in edible tissues of whitemouth croaker (Micropogonias furnieri) and meagre (Argyrosomus regius) captured in different Atlantic Ocean fishing areas and available to Brazilian and Portuguese consumers. Total PAH (∑PAHs) levels ranged from 1.32 to 5.41 μg/kg ww in wild-caught croaker and 2.66 (wild)-18.0 (farmed) μg/kg ww in meagre. Compounds with 2- and 3-rings represented 54-86% of ∑PAHs in the muscle tissues of wild-caught species (croaker and meagre) being naphthalene, fluorene, and phenanthrene the predominant compounds. ∑PAHs in farmed meagre were 4-7 times higher than in wild-caught meagre, with 2-, 3-, 4-, 5-, and 6-ring compounds representing 15, 18, 44, 22, and 1% of ∑PAHs, respectively. Benzo(a)pyrene levels in farmed meagre varied between 0.06 and 0.34 μg/kg ww. Crude oil refining and combustion sources were identified as the major sources of PAHs in FAO 27, 34 and 41 Atlantic Ocean fishing areas. The biometric characteristics (length, weight, moisture, and fat content) and ∑PAHs allowed to discriminate between wild-caught and farmed meagre samples and between meagre and croaker. Assessment of carcinogenic risks suggested that a diet exclusive on farmed meagre may pose additional risks for the health of European top consumers fish.

ACS Style

Marta Oliveira; Carolina De Gasperi Portella; Maria João Ramalhosa; Cristina Delerue-Matos; L.S. Sant’Ana; Simone Morais. Polycyclic aromatic hydrocarbons in wild and farmed whitemouth croaker and meagre from different Atlantic Ocean fishing areas: Concentrations and human health risk assessment. Food and Chemical Toxicology 2020, 146, 111797 .

AMA Style

Marta Oliveira, Carolina De Gasperi Portella, Maria João Ramalhosa, Cristina Delerue-Matos, L.S. Sant’Ana, Simone Morais. Polycyclic aromatic hydrocarbons in wild and farmed whitemouth croaker and meagre from different Atlantic Ocean fishing areas: Concentrations and human health risk assessment. Food and Chemical Toxicology. 2020; 146 ():111797.

Chicago/Turabian Style

Marta Oliveira; Carolina De Gasperi Portella; Maria João Ramalhosa; Cristina Delerue-Matos; L.S. Sant’Ana; Simone Morais. 2020. "Polycyclic aromatic hydrocarbons in wild and farmed whitemouth croaker and meagre from different Atlantic Ocean fishing areas: Concentrations and human health risk assessment." Food and Chemical Toxicology 146, no. : 111797.

Journal article
Published: 03 March 2020 in Molecules
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This study aimed to develop new canned chub mackerel products incorporating edible seaweeds (Ascophyllum nodosum, Fucus spiralis, Saccorhiza polyschides, Chondrus crispus, Porphyra sp. and Ulva sp.) harvested in the Portuguese North-Central coast, with simultaneous sensory improvement and minerals enrichment. Two processes were compared, namely the addition of seaweeds in i) the canning step and ii) in the brining step (as the replacement for salt). The concentrations of four macrominerals (Na, K, Ca and Mg), chloride, and twelve trace elements (Co, Cu, Fe, I, Li, Mn, Mo, Rb, Se, Sr, V and Zn) were determined by high-resolution continuum source flame atomic absorption spectrometry (HR-CS-FAAS) and inductively coupled plasma mass spectrometry (ICP-MS), respectively. Results showed that canned chub mackerel incorporating C. crispus and F. spiralis was found to be the preferred sensory option, also exhibiting contents enriched with Cl, Co, Cu, Fe, I, Li, Mg, Mn, Mo, Na, Rb, Se, and Sr. This effect was more pronounced when both seaweed species were added to replace the salt added in the brining step.

ACS Style

Elsa F. Vieira; Cristina Soares; Susana Machado; M. Teresa Oliva-Teles; Manuela Correia; Maria João Ramalhosa; Ana Carvalho; Valentina F. Domingues; Filipa Antunes; Simone Morais; Cristina Delerue-Matos. Development of New Canned Chub Mackerel Products Incorporating Edible Seaweeds—Influence on the Minerals and Trace Elements Composition. Molecules 2020, 25, 1133 .

AMA Style

Elsa F. Vieira, Cristina Soares, Susana Machado, M. Teresa Oliva-Teles, Manuela Correia, Maria João Ramalhosa, Ana Carvalho, Valentina F. Domingues, Filipa Antunes, Simone Morais, Cristina Delerue-Matos. Development of New Canned Chub Mackerel Products Incorporating Edible Seaweeds—Influence on the Minerals and Trace Elements Composition. Molecules. 2020; 25 (5):1133.

Chicago/Turabian Style

Elsa F. Vieira; Cristina Soares; Susana Machado; M. Teresa Oliva-Teles; Manuela Correia; Maria João Ramalhosa; Ana Carvalho; Valentina F. Domingues; Filipa Antunes; Simone Morais; Cristina Delerue-Matos. 2020. "Development of New Canned Chub Mackerel Products Incorporating Edible Seaweeds—Influence on the Minerals and Trace Elements Composition." Molecules 25, no. 5: 1133.

Conference paper
Published: 01 November 2019 in ICERI2019 Proceedings
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ACS Style

Olga Freitas; Maria João Ramalhosa; Cristina Soares; Abel Duarte; M. Fátima Barroso; Rita Alves; A.P. Carvalho; João Grosso; Lúcia Santos; Manuela M. Moreira; Cristina Delerure-Matos. ARTISTS CONNECTION BETWEEN RECYCLING AND ENVIRONMENTAL EDUCATION. ICERI2019 Proceedings 2019, 10636 -10643.

AMA Style

Olga Freitas, Maria João Ramalhosa, Cristina Soares, Abel Duarte, M. Fátima Barroso, Rita Alves, A.P. Carvalho, João Grosso, Lúcia Santos, Manuela M. Moreira, Cristina Delerure-Matos. ARTISTS CONNECTION BETWEEN RECYCLING AND ENVIRONMENTAL EDUCATION. ICERI2019 Proceedings. 2019; ():10636-10643.

Chicago/Turabian Style

Olga Freitas; Maria João Ramalhosa; Cristina Soares; Abel Duarte; M. Fátima Barroso; Rita Alves; A.P. Carvalho; João Grosso; Lúcia Santos; Manuela M. Moreira; Cristina Delerure-Matos. 2019. "ARTISTS CONNECTION BETWEEN RECYCLING AND ENVIRONMENTAL EDUCATION." ICERI2019 Proceedings , no. : 10636-10643.

Book chapter
Published: 22 February 2019 in Bottled and Packaged Water
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To best meet the consumer’s preferences, the beverages industry has been developing new nonalcoholic drinks. Actually, the drink production business has been focused on providing appealing (i.e., pleasant taste and aroma), high quality, and safe products to satisfy the increasing consumer demand for safety and nutritional excellence. Moreover, it is becoming increasingly clear that some drink components, for example, bioactive compounds, may be protective in some diseases caused by oxidative damage. Thus, there is a need to understand the physiological importance of bioactive compounds and also to know their amount and composition in different types of drinks. Considering this important issue, the purpose of this chapter is to review the characterization of bioactive compounds in flavored waters and fruit juices. Furthermore, it is also intended to describe selective assays for bioactive compounds assessment, such as spectrophotometric, DNA-based sensors, and chromatographic methods.

ACS Style

M. Fátima Barroso; Ana P. Carvalho; Manuela Correia; M.J. Ramalhosa; Cristina Delerue-Matos; Clara Grosso. Characterization of Bioactive Compounds in Flavored Waters and Fruit Juices. Bottled and Packaged Water 2019, 311 -366.

AMA Style

M. Fátima Barroso, Ana P. Carvalho, Manuela Correia, M.J. Ramalhosa, Cristina Delerue-Matos, Clara Grosso. Characterization of Bioactive Compounds in Flavored Waters and Fruit Juices. Bottled and Packaged Water. 2019; ():311-366.

Chicago/Turabian Style

M. Fátima Barroso; Ana P. Carvalho; Manuela Correia; M.J. Ramalhosa; Cristina Delerue-Matos; Clara Grosso. 2019. "Characterization of Bioactive Compounds in Flavored Waters and Fruit Juices." Bottled and Packaged Water , no. : 311-366.

Journal article
Published: 01 December 2018 in Food Chemistry
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The total protein content and the (total and free) amino acid composition of nine edible species of red, brown and green seaweeds collected in the Portuguese North-Central coast were quantified to assess their potential contribution to the recommended dietary intake. Whenever possible, the protein and amino acid composition was compared with that of commercial European seaweeds. The protein content was the highest (P<0.05) in red species (19.1-28.2 g/100g dw), followed by the green seaweed Ulva spp. (20.5-23.3 g/100g dw), with the lowest content found in brown seaweeds (6.90-19.5 g/100g dw). Brown seaweeds presented the lowest mean contents of essential amino acids (EAAs) (41.0% protein) but significantly (P<0.05) higher concentrations of non-essential amino acids (36.1% protein) and free amino acids (6.47-24.0% protein). Tryptophan, methionine and leucine were the limiting EAAs in all species. In contrast, lysine was found in high concentrations, especially in red (2.71-3.85% protein) and green (2.84-4.24% protein) seaweeds.

ACS Style

Elsa Ferreira Vieira; Cristina Soares; Susana Machado; Manuela Correia; Maria João Ramalhosa; Maria Teresa Oliva-Teles; Ana Carvalho; Valentina Domingues; Filipa Antunes; Teresa Azevedo Cardoso Oliveira; Simone Morais; Cristina Delerue-Matos. Seaweeds from the Portuguese coast as a source of proteinaceous material: Total and free amino acid composition profile. Food Chemistry 2018, 269, 264 -275.

AMA Style

Elsa Ferreira Vieira, Cristina Soares, Susana Machado, Manuela Correia, Maria João Ramalhosa, Maria Teresa Oliva-Teles, Ana Carvalho, Valentina Domingues, Filipa Antunes, Teresa Azevedo Cardoso Oliveira, Simone Morais, Cristina Delerue-Matos. Seaweeds from the Portuguese coast as a source of proteinaceous material: Total and free amino acid composition profile. Food Chemistry. 2018; 269 ():264-275.

Chicago/Turabian Style

Elsa Ferreira Vieira; Cristina Soares; Susana Machado; Manuela Correia; Maria João Ramalhosa; Maria Teresa Oliva-Teles; Ana Carvalho; Valentina Domingues; Filipa Antunes; Teresa Azevedo Cardoso Oliveira; Simone Morais; Cristina Delerue-Matos. 2018. "Seaweeds from the Portuguese coast as a source of proteinaceous material: Total and free amino acid composition profile." Food Chemistry 269, no. : 264-275.

Journal article
Published: 10 September 2018 in Food and Chemical Toxicology
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Polycyclic aromatic hydrocarbons (PAHs) are persistent pollutants that have been raising global concern due to their carcinogenic and mutagenic properties. A total of 18 PAHs (16 USEPA priority compounds, benzo(j)fluoranthene and dibenzo(a,l)pyrene) were assessed in the edible tissues of raw octopus (Octopus vulgaris, Octopus maya, and Eledone cirrhosa) from six geographical origins available to Portuguese consumers. Inter- and intra-species comparison was statistically performed. The concentrations of total PAHs (∑PAHs) ranged between 8.59 and 12.8 μg/kg w.w. Octopus vulgaris caught in northwest Atlantic Ocean presented ΣPAHs significantly higher than those captured in Pacific Ocean and Mediterranean Sea, as well as than the other characterized species from western central and northeast Atlantic Ocean. PAHs with 2–3 rings were the predominant compounds (86–92% of ∑PAHs) but diagnostic ratios indicated the existence of pyrogenic sources in addition to petrogenic sources. Known and possible/probable carcinogenic compounds represented 11–21% of ΣPAHs. World and Portuguese per capita ingestion of ∑PAHs due to cephalopods consumption varied between 1.62-2.55 × 10−4 and 7.09-11.2 × 10−4 μg/kg body weight per day, respectively. Potential risks estimated for low and high consumers according to USEPA methodology suggested that a regular consumption of raw octopus does not pose public health risks.

ACS Style

Marta Oliveira; Filipa O. Gomes; Álvaro Torrinha; Maria João Ramalhosa; Cristina Delerue-Matos; Simone Morais. Commercial octopus species from different geographical origins: Levels of polycyclic aromatic hydrocarbons and potential health risks for consumers. Food and Chemical Toxicology 2018, 121, 272 -282.

AMA Style

Marta Oliveira, Filipa O. Gomes, Álvaro Torrinha, Maria João Ramalhosa, Cristina Delerue-Matos, Simone Morais. Commercial octopus species from different geographical origins: Levels of polycyclic aromatic hydrocarbons and potential health risks for consumers. Food and Chemical Toxicology. 2018; 121 ():272-282.

Chicago/Turabian Style

Marta Oliveira; Filipa O. Gomes; Álvaro Torrinha; Maria João Ramalhosa; Cristina Delerue-Matos; Simone Morais. 2018. "Commercial octopus species from different geographical origins: Levels of polycyclic aromatic hydrocarbons and potential health risks for consumers." Food and Chemical Toxicology 121, no. : 272-282.

Journal article
Published: 01 September 2018 in Science of The Total Environment
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Since annually a high amount of wastes is produced in vine pruning, the aim of this study was to evaluate the potential of vine shoots from two Portuguese grape varieties (Touriga Nacional - TN and Tinta Roriz - TR) to be used as a natural source of phenolic compounds. To reach this goal, three techniques were explored, namely microwave-assisted extraction (MAE), subcritical water extraction (SWE) and conventional extraction (CE). The phenolic composition of the extracts, antioxidant and biological activities were evaluated by spectrophotometry and chromatography. MAE and SWE produced the highest concentrated extracts. TR vine shoot variety had the highest antioxidant activity and total phenolic (32.1±0.9mggallicacidequivalents/g dry sample), as well as flavonoid content (18.7±1.2mgepicatechinequivalents/g dry sample). For the first time, the biological activity of the vine shoot extracts was tested. Results demonstrated that all of them had antimicrobial potential against different bacteria and yeasts, and the ability of inhibiting α-amylase and acetylcholinesterase enzymes, with MAE TR extracts being the most efficient. HPLC analysis enabled the identification of different phenolic compounds, with gallic acid, catechin, myricetin and kaempferol-3-O-rutinoside being the main contributors to the phenolic composition. Portuguese vine shoot wastes could serve as easily accessible source of natural antioxidants for the food or pharmaceutical industries.

ACS Style

Manuela M. Moreira; M. Fátima Barroso; João Vasconcellos Porto; Maria João Ramalhosa; Jaroslava Švarc-Gajić; Maria Leticia Miranda Fernandes Estevinho; Simone Morais; Cristina Delerue-Matos. Potential of Portuguese vine shoot wastes as natural resources of bioactive compounds. Science of The Total Environment 2018, 634, 831 -842.

AMA Style

Manuela M. Moreira, M. Fátima Barroso, João Vasconcellos Porto, Maria João Ramalhosa, Jaroslava Švarc-Gajić, Maria Leticia Miranda Fernandes Estevinho, Simone Morais, Cristina Delerue-Matos. Potential of Portuguese vine shoot wastes as natural resources of bioactive compounds. Science of The Total Environment. 2018; 634 ():831-842.

Chicago/Turabian Style

Manuela M. Moreira; M. Fátima Barroso; João Vasconcellos Porto; Maria João Ramalhosa; Jaroslava Švarc-Gajić; Maria Leticia Miranda Fernandes Estevinho; Simone Morais; Cristina Delerue-Matos. 2018. "Potential of Portuguese vine shoot wastes as natural resources of bioactive compounds." Science of The Total Environment 634, no. : 831-842.

Journal article
Published: 01 July 2018 in Environmental Research
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This work aimed to determine the effect of culinary practices on the contamination level and bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in seafood. The selected farmed seafood species (marine shrimp, clams and seaweed) were commercially available in Portugal. The mean concentrations of PAHs varied between 0.23 and 51.8 µg kg-1, with the lowest value being observed in raw shrimp and the highest in dried seaweed. The number of compounds detected in seaweed and clams (naphthalene, acenaphthene, fluorene, phenanthrene, benzo(b)fluoranthene and benzo(j)fluoranthene) were higher than in shrimp (fluorene and pyrene). Among the PAHs measured, fluorene was the predominant one. There was a significant interaction effect between species and culinary treatment (p < 0.05), thus boiled and dried seaweed samples presented the lowest and the highest levels of fluorene (0.13 and 1.8 µg kg-1), respectively. The daily intake of PAHs decreased with bioaccessibility, varying from 22% for benzo(k)fluoranthene (in raw clam) to 84% for phenanthrene (in steamed clam). According to the potency equivalent concentrations, screening values and bioaccessibility of PAHs, the consumption of marine shrimp, clam and seaweed is considered as safe for consumers.

ACS Style

Fabíola Helena Dos Santos Fogaça; Cristina Soares; Marta Oliveira; Ricardo N. Alves; Ana Luísa Maulvault; Vera Liane Barbosa; Patrícia Anacleto; João Avelar Magalhães; Narcisa M. Bandarra; Maria João Ramalhosa; Simone Morais; António Marques. Polycyclic aromatic hydrocarbons bioaccessibility in seafood: Culinary practices effects on dietary exposure. Environmental Research 2018, 164, 165 -172.

AMA Style

Fabíola Helena Dos Santos Fogaça, Cristina Soares, Marta Oliveira, Ricardo N. Alves, Ana Luísa Maulvault, Vera Liane Barbosa, Patrícia Anacleto, João Avelar Magalhães, Narcisa M. Bandarra, Maria João Ramalhosa, Simone Morais, António Marques. Polycyclic aromatic hydrocarbons bioaccessibility in seafood: Culinary practices effects on dietary exposure. Environmental Research. 2018; 164 ():165-172.

Chicago/Turabian Style

Fabíola Helena Dos Santos Fogaça; Cristina Soares; Marta Oliveira; Ricardo N. Alves; Ana Luísa Maulvault; Vera Liane Barbosa; Patrícia Anacleto; João Avelar Magalhães; Narcisa M. Bandarra; Maria João Ramalhosa; Simone Morais; António Marques. 2018. "Polycyclic aromatic hydrocarbons bioaccessibility in seafood: Culinary practices effects on dietary exposure." Environmental Research 164, no. : 165-172.

Conference paper
Published: 18 September 2017 in IOP Conference Series: Materials Science and Engineering
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The Portuguese coast presents a large amount of potentially edible seaweeds that are underexploited. The identification of different macroalgae species and their availability in the northern and central coast of the continental territory was assessed. The nutritional value of seaweeds is discussed based on a literature review (when available) focused on data for species collected in Portugal with the aim to define the most important nutritional parameters that should be characterized in the samples. Possible health concerns related with the presence of contaminants are also considered.

ACS Style

C. Soares; S. Machado; E.F. Vieira; S. Morais; M.T. Teles; M. Correia; A. Carvalho; V. F. Domingues; M.J. Ramalhosa; C. Delerue-Matos; F. Antunes. Seaweeds from the Portuguese coast: A potential food resource? IOP Conference Series: Materials Science and Engineering 2017, 231, 012126 .

AMA Style

C. Soares, S. Machado, E.F. Vieira, S. Morais, M.T. Teles, M. Correia, A. Carvalho, V. F. Domingues, M.J. Ramalhosa, C. Delerue-Matos, F. Antunes. Seaweeds from the Portuguese coast: A potential food resource? IOP Conference Series: Materials Science and Engineering. 2017; 231 (1):012126.

Chicago/Turabian Style

C. Soares; S. Machado; E.F. Vieira; S. Morais; M.T. Teles; M. Correia; A. Carvalho; V. F. Domingues; M.J. Ramalhosa; C. Delerue-Matos; F. Antunes. 2017. "Seaweeds from the Portuguese coast: A potential food resource?" IOP Conference Series: Materials Science and Engineering 231, no. 1: 012126.

Journal article
Published: 01 October 2016 in Food Control
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Nine plant infusions were assessed in terms of their polyphenolic compounds and antioxidant capacity properties by performing a “battery” of assays. Bioactive compounds (total phenolic and flavonoid content) were measured using spectrophotometric procedures and the total antioxidant capacity was determined by DPPH●-RSA, FRAP and DNA-based biosensor against the reactive oxygen species: HO● and H2O2. Although the optical methods are based on the donation of hydrogen or electrons between antioxidants and radicals, the DNA-based biosensor intends to simulate an in vivo assay by exposing DNA layer to biological ROS and antioxidants. In general, green and black tea showed higher polyphenolic content and antioxidant capacity evaluated by the described methods. All the analysed plant beverages showed to be a good source of bioactive compounds, particularly the green and black tea, peppermint and senna.

ACS Style

M. Fátima Barroso; Maria João Ramalhosa; Rita C. Alves; Alexandra Dias; Cristina M.D. Soares; Maria Teresa Oliva-Teles; Cristina Delerue-Matos. Total antioxidant capacity of plant infusions: Assessment using electrochemical DNA-based biosensor and spectrophotometric methods. Food Control 2016, 68, 153 -161.

AMA Style

M. Fátima Barroso, Maria João Ramalhosa, Rita C. Alves, Alexandra Dias, Cristina M.D. Soares, Maria Teresa Oliva-Teles, Cristina Delerue-Matos. Total antioxidant capacity of plant infusions: Assessment using electrochemical DNA-based biosensor and spectrophotometric methods. Food Control. 2016; 68 ():153-161.

Chicago/Turabian Style

M. Fátima Barroso; Maria João Ramalhosa; Rita C. Alves; Alexandra Dias; Cristina M.D. Soares; Maria Teresa Oliva-Teles; Cristina Delerue-Matos. 2016. "Total antioxidant capacity of plant infusions: Assessment using electrochemical DNA-based biosensor and spectrophotometric methods." Food Control 68, no. : 153-161.

Journal article
Published: 03 April 2016 in International Journal of Food Science & Technology
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This study aimed to evaluate the effect of microwave-assisted extraction (MAE) parameters on the composition and bioactivity of goji (Lycium barbarum) extracts. Extracts were obtained under a central composite design combination of experimental conditions, and characterised through HPLC-DAD; their bioactive capacity was ascertained for antimicrobial and antioxidant capacity, the later by spectrophotometric [2,2-azinobis (3- ethylbenzothiazoline-6-sulfonic acid) diammonium salt-radical scavenging activity assay – 413–748 mg ascorbic acid equivalents/100 g DW and oxygen radical absorbance capacity – 1901–2292 mg trolox equivalents/100 g DW] and electrochemical (DNA-based sensor – 3571–6602 mg ascorbic acid/100 g DW) methods. The quantitative profile of phenolic compounds was strongly dependent on MAE conditions. Significant correlations were found between the presence of several flavonoids and solvent composition, as well as between phenolic acids with methoxy group and the response to DNA-based sensor. Results may improve targeted extractions for specific compounds, leading to the achievement of extracts richer in antioxidant capacity, as well as in the tailoring of the biosensor response sensitivity to the composition of the extracts under analysis.info:eu-repo/semantics/publishedVersio

ACS Style

Ana P. Carvalho; Marta Mendes; Manuela M. Moreira; Diana Cruz; Júlia M. C. S. Magalhães; M. Fátima Barroso; M. J. Ramalhosa; Abel J. Duarte; Luis Guido; Ana M. Gomes; Cristina Delerue Matos. Microwave-assisted extraction in goji berries: effect on composition and bioactivity, evaluated through conventional and nonconventional methodologies. International Journal of Food Science & Technology 2016, 51, 1401 -1408.

AMA Style

Ana P. Carvalho, Marta Mendes, Manuela M. Moreira, Diana Cruz, Júlia M. C. S. Magalhães, M. Fátima Barroso, M. J. Ramalhosa, Abel J. Duarte, Luis Guido, Ana M. Gomes, Cristina Delerue Matos. Microwave-assisted extraction in goji berries: effect on composition and bioactivity, evaluated through conventional and nonconventional methodologies. International Journal of Food Science & Technology. 2016; 51 (6):1401-1408.

Chicago/Turabian Style

Ana P. Carvalho; Marta Mendes; Manuela M. Moreira; Diana Cruz; Júlia M. C. S. Magalhães; M. Fátima Barroso; M. J. Ramalhosa; Abel J. Duarte; Luis Guido; Ana M. Gomes; Cristina Delerue Matos. 2016. "Microwave-assisted extraction in goji berries: effect on composition and bioactivity, evaluated through conventional and nonconventional methodologies." International Journal of Food Science & Technology 51, no. 6: 1401-1408.

Journal article
Published: 01 March 2016 in Journal of Chromatography A
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An analytical method based on a modified Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) extraction procedure followed by ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) was developed for the analysis of psychiatric drugs in sediments. An optimized approach was applied in sample preparation by using basic acetonitrile as extraction solvent. Extraction was followed by a clean-up using dispersive solid phase extraction (dSPE) to remove matrix interfering compounds. The analytical method was validated in terms of sensitivity, linearity, recovery, intra- and inter-day precisions and method detection and quantification limits. Under optimized conditions, limits of detection ranged from 0.01 ng g−1 to 2.08 ng g−1; and recoveries between 47 and 110% with relative standard deviation (RSD) below 5%. The developed methodology was applied to sediments of two Portuguese rivers (Douro and Lima rivers) and nine out of eleven psychiatric drugs were detected in sediments at concentrations up to 26.4 ng g−1 (dry weight). To the best of our knowledge, it was the first time that the human metabolites norfluoxetine and norsertraline were detected in river sediments at levels of few nanograms per gram.info:eu-repo/semantics/publishedVersio

ACS Style

Lúcia H.M.L.M. Santos; Maria João Ramalhosa; Marta Ferreira; Cristina Delerue-Matos. Development of a modified acetonitrile-based extraction procedure followed by ultra-high performance liquid chromatography–tandem mass spectrometry for the analysis of psychiatric drugs in sediments. Journal of Chromatography A 2016, 1437, 37 -48.

AMA Style

Lúcia H.M.L.M. Santos, Maria João Ramalhosa, Marta Ferreira, Cristina Delerue-Matos. Development of a modified acetonitrile-based extraction procedure followed by ultra-high performance liquid chromatography–tandem mass spectrometry for the analysis of psychiatric drugs in sediments. Journal of Chromatography A. 2016; 1437 ():37-48.

Chicago/Turabian Style

Lúcia H.M.L.M. Santos; Maria João Ramalhosa; Marta Ferreira; Cristina Delerue-Matos. 2016. "Development of a modified acetonitrile-based extraction procedure followed by ultra-high performance liquid chromatography–tandem mass spectrometry for the analysis of psychiatric drugs in sediments." Journal of Chromatography A 1437, no. : 37-48.

Journal article
Published: 01 February 2016 in Journal of Electroanalytical Chemistry
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ACS Style

Diana Cruz; M. Fátima Barroso; Maria João Ramalhosa; Andreia Coelho; Hélder Da Silva; Abel J. Duarte; Maria Begoña González-García; Ana P. Carvalho; Cristina Delerue-Matos. DNA-based sensor against nitrite oxide radical: Evaluation of total antioxidant capacity in beverages. Journal of Electroanalytical Chemistry 2016, 763, 110 -115.

AMA Style

Diana Cruz, M. Fátima Barroso, Maria João Ramalhosa, Andreia Coelho, Hélder Da Silva, Abel J. Duarte, Maria Begoña González-García, Ana P. Carvalho, Cristina Delerue-Matos. DNA-based sensor against nitrite oxide radical: Evaluation of total antioxidant capacity in beverages. Journal of Electroanalytical Chemistry. 2016; 763 ():110-115.

Chicago/Turabian Style

Diana Cruz; M. Fátima Barroso; Maria João Ramalhosa; Andreia Coelho; Hélder Da Silva; Abel J. Duarte; Maria Begoña González-García; Ana P. Carvalho; Cristina Delerue-Matos. 2016. "DNA-based sensor against nitrite oxide radical: Evaluation of total antioxidant capacity in beverages." Journal of Electroanalytical Chemistry 763, no. : 110-115.

Journal article
Published: 01 February 2015 in Journal of Hazardous Materials
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Sertraline is widely prescribed worldwide and frequently detected in aquatic systems. There is, however, a remarkable gap of information on its potential impact on estuarine and coastal invertebrates. This study investigated sertraline accumulation and effects in Carcinus maenas. Crabs from a moderately contaminated (Lima) and a low-impacted (Minho) estuary were exposed to environmental and high levels of sertraline (0.05, 5, 500 μg L(-1)). A battery of biomarkers related to sertraline mode of action was employed to assess neurotransmission, energy metabolism, biotransformation and oxidative stress pathways. After a seven-day exposure, sertraline accumulation in crabs' soft tissues was found in Lima (5 μg L(-1): 15.3 ng L(-1) ww; 500 μg L(-1): 1010 ng L(-1) ww) and Minho (500 μg L(-1): 605 ng L(-1) ww) animals. Lima crabs were also more sensitive to sertraline than those from Minho, exhibiting decreased acetylcholinesterase activity, indicative of ventilatory and locomotory dysfunction, inhibition of anti-oxidant enzymes and increased oxidative damage at ≥ 0.05 μg L(-1). The Integrated Biomarker Response (IBR) index indicated their low health status. In addition, Minho crabs showed non-monotonic responses of acetylcholinesterase suggestive of hormesis. The results pointed an influence of the exposure history on differential sensitivity to sertraline and the need to perform evaluations with site-specific ecological receptors to increase relevance of risk estimations when extrapolating from laboratory to field conditions.

ACS Style

Aurélie P. Rodrigues; Lúcia Santos; Maria João Ramalhosa; Cristina Delerue-Matos; Laura Guimarães. Sertraline accumulation and effects in the estuarine decapod Carcinus maenas: Importance of the history of exposure to chemical stress. Journal of Hazardous Materials 2015, 283, 350 -358.

AMA Style

Aurélie P. Rodrigues, Lúcia Santos, Maria João Ramalhosa, Cristina Delerue-Matos, Laura Guimarães. Sertraline accumulation and effects in the estuarine decapod Carcinus maenas: Importance of the history of exposure to chemical stress. Journal of Hazardous Materials. 2015; 283 ():350-358.

Chicago/Turabian Style

Aurélie P. Rodrigues; Lúcia Santos; Maria João Ramalhosa; Cristina Delerue-Matos; Laura Guimarães. 2015. "Sertraline accumulation and effects in the estuarine decapod Carcinus maenas: Importance of the history of exposure to chemical stress." Journal of Hazardous Materials 283, no. : 350-358.

Thematic issue
Published: 21 May 2014 in Environmental Earth Sciences
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The excessive use of pesticides and fertilisers in agriculture has generated a decrease in groundwater and surface water quality in many regions of the EU, constituting a hazard for human health and the environment. Besides, on-site sewage disposal is an important source of groundwater contamination in urban and peri-urban areas. The assessment of groundwater vulnerability to contamination is an important tool to fulfil the demands of EU Directives. The purpose of this study is to assess the groundwater vulnerability to contamination related mainly to agricultural activities in a peri-urban area (Vila do Conde, NW Portugal). The hydrogeological framework is characterised mainly by fissured granitic basement and sedimentary cover. Water samples were collected and analysed for temperature, pH, electrical conductivity, chloride, phosphate, nitrate and nitrite. An evaluation of groundwater vulnerability to contamination was applied (GOD-S, Pesticide DRASTIC-Fm, SINTACS and SI) and the potential nitrate contamination risk was assessed, both on a hydrogeological GIS-based mapping. A principal component analysis was performed to characterised patterns of relationship among groundwater contamination, vulnerability, and the hydrogeological setting assessed. Levels of nitrate above legislation limits were detected in 75 % of the samples analysed. Alluvia units showed the highest nitrate concentrations and also the highest vulnerability and risk. Nitrate contamination is a serious problem affecting groundwater, particularly shallow aquifers, especially due to agriculture activities, livestock and cesspools. GIS-based cartography provided an accurate way to improve knowledge on water circulation models and global functioning of local aquifer systems. Finally, this study highlights the adequacy of an integrated approach, combining hydrogeochemical data, vulnerability assessments and multivariate analysis, to understand groundwater processes in peri-urban areas.

ACS Style

M. Fátima Barroso; M. J. Ramalhosa; A. Olhero; M. C. Antão; M. F. Pina; Laura Guimaraes; J. Teixeira; M. J. Afonso; Cristina Delerue-Matos; H. I. Chaminé. Assessment of groundwater contamination in an agricultural peri-urban area (NW Portugal): an integrated approach. Environmental Earth Sciences 2014, 73, 2881 -2894.

AMA Style

M. Fátima Barroso, M. J. Ramalhosa, A. Olhero, M. C. Antão, M. F. Pina, Laura Guimaraes, J. Teixeira, M. J. Afonso, Cristina Delerue-Matos, H. I. Chaminé. Assessment of groundwater contamination in an agricultural peri-urban area (NW Portugal): an integrated approach. Environmental Earth Sciences. 2014; 73 (6):2881-2894.

Chicago/Turabian Style

M. Fátima Barroso; M. J. Ramalhosa; A. Olhero; M. C. Antão; M. F. Pina; Laura Guimaraes; J. Teixeira; M. J. Afonso; Cristina Delerue-Matos; H. I. Chaminé. 2014. "Assessment of groundwater contamination in an agricultural peri-urban area (NW Portugal): an integrated approach." Environmental Earth Sciences 73, no. 6: 2881-2894.

Comparative study
Published: 30 May 2013 in Food and Chemical Toxicology
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The concentrations of 18 polycyclic aromatic hydrocarbons (PAHs) were determined in five commercially valuable squid species from different geographical origins (Atlantic, Indic and Pacific Oceans). Out of the 18 quantified PAHs (the 16 PAHs considered by US EPA as priority pollutants, dibenzo(a,l)pyrene and benzo(j)fluoranthene) only dibenz(a,h)anthracene was not detected. The total concentrations of PAHs varied by a factor of more than 100-fold, from 0.22 (Loligo gahi) to 60.9 μg/kg ww (Loligo reynaudii). Intra- and inter-specific variability of PAH levels was statistically assessed. Nine carcinogenic (probable/possible) PAHs accounted for 1% (L. reynaudii) to 26% (Loligo opalescens) of the total PAHs content being the main contributors naphthalene (in Loligo duvaucelii, L. reynaudii and Loligo vulgaris species), chrysene (in L. opalescens) and indeno(1,2,3-cd)pyrene (in L. gahi). PAHs source analysis indicated that four of the five zones of capture of the different squid species are significantly affected by both petrogenic and pyrolytic sources. Assessment of the target carcinogenic risks, established by the US EPA, suggested that L. gahi (Atlantic Ocean) and L. opalescens (from Pacific Ocean) may pose additional risks for consumers, if not eaten in moderation, derived from benzo(a)pyrene ingestion.

ACS Style

Filipa O. Gomes; Marta Oliveira; Maria João Ramalhosa; Cristina Delerue-Matos; Simone Morais. Polycyclic aromatic hydrocarbons in commercial squids from different geographical origins: Levels and risks for human consumption. Food and Chemical Toxicology 2013, 59, 46 -54.

AMA Style

Filipa O. Gomes, Marta Oliveira, Maria João Ramalhosa, Cristina Delerue-Matos, Simone Morais. Polycyclic aromatic hydrocarbons in commercial squids from different geographical origins: Levels and risks for human consumption. Food and Chemical Toxicology. 2013; 59 ():46-54.

Chicago/Turabian Style

Filipa O. Gomes; Marta Oliveira; Maria João Ramalhosa; Cristina Delerue-Matos; Simone Morais. 2013. "Polycyclic aromatic hydrocarbons in commercial squids from different geographical origins: Levels and risks for human consumption." Food and Chemical Toxicology 59, no. : 46-54.

Journal article
Published: 01 November 2012 in Food Chemistry
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Maria João Ramalhosa; Paula Paíga; Simone Morais; Ana M.M. Sousa; Maria Gonçalves; Cristina Delerue-Matos; Beatriz Oliveira. Analysis of polycyclic aromatic hydrocarbons in fish: Optimisation and validation of microwave-assisted extraction. Food Chemistry 2012, 135, 234 -242.

AMA Style

Maria João Ramalhosa, Paula Paíga, Simone Morais, Ana M.M. Sousa, Maria Gonçalves, Cristina Delerue-Matos, Beatriz Oliveira. Analysis of polycyclic aromatic hydrocarbons in fish: Optimisation and validation of microwave-assisted extraction. Food Chemistry. 2012; 135 (1):234-242.

Chicago/Turabian Style

Maria João Ramalhosa; Paula Paíga; Simone Morais; Ana M.M. Sousa; Maria Gonçalves; Cristina Delerue-Matos; Beatriz Oliveira. 2012. "Analysis of polycyclic aromatic hydrocarbons in fish: Optimisation and validation of microwave-assisted extraction." Food Chemistry 135, no. 1: 234-242.

Journal article
Published: 01 March 2012 in Food Chemistry
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In this work, a microwave-assisted extraction (MAE) methodology was compared with several conventional extraction methods (Soxhlet, Bligh & Dyer, modified Bligh & Dyer, Folch, modified Folch, Hara & Radin, Roese-Gottlieb) for quantification of total lipid content of three fish species: horse mackerel (Trachurus trachurus), chub mackerel (Scomber japonicus), and sardine (Sardina pilchardus). The influence of species, extraction method and frozen storage time (varying from fresh to 9 months of freezing) on total lipid content was analysed in detail. The efficiencies of methods MAE, Bligh & Dyer, Folch, modified Folch and Hara & Radin were the highest and although they were not statistically different, differences existed in terms of variability, with MAE showing the highest repeatability (CV = 0.034). Roese-Gottlieb, Soxhlet, and modified Bligh & Dyer methods were very poor in terms of efficiency as well as repeatability (CV between 0.13 and 0.18)

ACS Style

Maria João Ramalhosa; Paula Paíga; Simone Morais; Manuel Alves; Cristina Delerue-Matos; Beatriz Oliveira. Lipid content of frozen fish: Comparison of different extraction methods and variability during freezing storage. Food Chemistry 2012, 131, 328 -336.

AMA Style

Maria João Ramalhosa, Paula Paíga, Simone Morais, Manuel Alves, Cristina Delerue-Matos, Beatriz Oliveira. Lipid content of frozen fish: Comparison of different extraction methods and variability during freezing storage. Food Chemistry. 2012; 131 (1):328-336.

Chicago/Turabian Style

Maria João Ramalhosa; Paula Paíga; Simone Morais; Manuel Alves; Cristina Delerue-Matos; Beatriz Oliveira. 2012. "Lipid content of frozen fish: Comparison of different extraction methods and variability during freezing storage." Food Chemistry 131, no. 1: 328-336.