Combining molecular imprinted polymers and water-soluble manganese-doped zinc sulfide quantum dots (Mn²⁺: ZnS QDs), a new molecule imprinted polymers-based fluorescence sensor was designed. The molecule imprinted quantum dots (MIP@QDs) were constructed by coating molecular imprinted polymers layer on the surface of ZnS: Mn²⁺ QDs using the surface molecular imprinting technology. The developed MIP@QDs-based sensor was used for rapid and selective fluorescence sensing of sulfanilamide in water samples. The binding experiments showed that the MIP@QDs has rapid fluorescent responses, which are highly selective of and sensitive to the detection of sulfanilamide. The respond time of the MIP@QDs was 5 min, and the imprinting factor was 14.8. Under optimal conditions, the developed MIP@QDs-based sensor shows a good linearity (R² = 0.9916) over a sulfanilamide concentration range from 2.90 × 10⁻⁸ to 2.90 × 10⁻⁶ mol L⁻¹, with a detection limit of 3.23 × 10⁻⁹ mol L⁻¹. Furthermore, the proposed MIP@QDs-based sensor was applied to the determination of sulfanilamide in real samples, with recoveries of 96.80%–104.33%, exhibiting good recyclability and stability. Experimental results showed that the prepared MIP@QDs has the potential to serve as a selective and sensitive sensor for the fluorescence sensing of sulfonamides in water samples.

Aiming to understand the genetic diversity of Culter and Cultrichthys fishes in West Dongting Lake following the implementation of fishing ban, this study analyzed the genetic diversity and phylogeny of five Cultrinae species based on mitochondrial Cytb and COI gene sequences. For Cytb, 50 haplotypes were identified with high overall genetic diversity (H_d = 0.954, π = 0.04765, K = 53.506). Similarly, COI yielded 48 haplotypes (H_d = 0.950, π = 0.03251, K = 36.414). Interspecific comparisons revealed that Culter mongolicus and Culter alburnus exhibited the highest genetic diversity, while Cultrichthys erythropterus showed the lowest. Demographic inferences from neutrality tests and mismatch distribution analyses differed between markers. Based on Cytb, populations of C. erythropterus and C. oxycephaloides appeared to be at neutrality, whereas C. mongolicus, C. alburnus, and C. dabryi significantly deviated, suggesting historical expansion. Mismatch distributions supported recent expansion in C. mongolicus and C. dabryi. In contrast, COI-based neutrality tests indicated expansion in C. mongolicus, C. alburnus, and C. dabryi, with mismatch results confirming this signal for C. dabryi. The negative values of Tajima’s D and Fu’s Fs observed across all five species reflect an accumulation of low-frequency alleles within populations, which may indicate a recent rapid demographic recovery. These findings provide genetic evidence that the genetic diversity of these Cultrinae species has likely been recovering since the fishing ban took effect on 1 January 2020. Further conservation strategies are therefore recommended to promote population recovery and enhance genetic diversity.

Achalinus sheni Ma, Xu, Qi, Wang, Tang, Huang & Jiang, 2023 was originally described based on only male specimens from Lianyuan City and Nanyue District, Hunan, China. So far, no information on the females of this species is available. After molecular systematics and morphological characters of recently collected specimens from Xinshao County, Hunan, several specimens (3 males and 1 female) were identified as this species. Therefore, we provided supplementary descriptions of the female characteristics of this species in this study. Meanwhile, we extended the description of this species.

Achalinus sheni Ma, Xu, Qi, Wang, Tang, Huang & Jiang, 2023 was originally described based on only male specimens from Lianyuan City and Nanyue District, Hunan, China. So far, no information on the females of this species is available. After molecular systematics and morphological characters of recently collected specimens from Xinshao County, Hunan, several specimens (3 males and 1 female) were identified as this species. Therefore, we provided supplementary descriptions of the female characteristics of this species in this study. Meanwhile, we extended the description of this species.

This study describes a new species of the newt genus (Tylototriton sheni sp. nov.) from the Huangyan area in Zhongfang County of Hunan Province, China, based on morphological and molecular genetic evidence. Phylogenetic analyses based on mitochondrial 16S rRNA and ND2 gene sequences revealed that the new species forms a monophyletic clade with T. maolanensis and T. broadoridgus, all belonging to the T. asperrimus species group. The new species diagnosed by a combination of following characters: (1) Medium-sized body (SVL 60.9–70.1 mm in males and 74.6–75.5 mm in females); (2) Vomerine teeth in a wide inverted V shape, tips separated; (3) Tubercles on dorsolateral body disconnected, total munbers 16–18; (4) Head length greater than head width; (5) The posterolateral part of the squamosal bears a distinct process extending beyond the posterior margin of the exoccipital; (6) Tips of forelimbs and hindlimbs reaching and slightly overlapping when folded towards the body; (7) Tail shorter than body (TAL/SVL = 0.79–0.91 in males, 0.72–0.75 in females); (8) Fingertips extending beyond the tip of the snout when the forelimbs are stretched forward. This paper summarizes the developmental data of embryos of the new taxon. We propose that the conservation status of the new species should be Critically Endangered (CR) following the IUCN criteria (B1ab iii), and the new species should be included in the List of State Key Protected Wild Animals of China. Immediate protection and supervision of its population are recommended.