The Croatian olive (Olea europaea L.) cultivar, ‘Lastovka’ is mainly used for oil production. While fruit yields are high and regular, the growing area is limited due to its sensitivity to low temperatures, with production concentrated on the South Dalmatian island of Korčula. Different morphological types have been observed during production. In order to determine the extent of intra-cultivar diversity, 47 individual ‘Lastovka’ trees were analysed for 11 morphological traits and assessed using AFLP markers, and compared to ten trees each of the cultivars ‘Oblica’ and ‘Drobnica’. Clear differentiation among cultivars was detected, and the results showed that both the morphological and molecular data were equally reliable for discriminating between cultivars. The existence of intra-cultivar diversity in ‘Lastovka’ was therefore confirmed at both the morphological and molecular levels, providing an opportunity for the selection of potentially superior clones.

Olive growing in Croatia has a long tradition and is of great economic and social impact. The present study includes a set of 108 tree samples (88 samples corresponding to 60 presumed cultivars and 20 trees of unnamed ones) collected from 27 groves in the entire olive growing area, and is the most comprehensive survey to be conducted in Croatia. The genetic diversity, relationships, and structures of olive plants were studied using eight microsatellite loci. All loci were polymorphic and revealed a total of 90 alleles. A total of 74 different genotypes were detected that were subjected to further diversity and genetic relationship studies. The Fitch–Margoliash tree and Bayesian analysis of population structure revealed a complex relationship between the identified olive genotypes, which were clustered into three gene pools, indicating different origins of Croatian olive germplasms. Excluding the redundant germplasms, 44 different genotypes among the sampled trees of well-known cultivars and 16 new local germplasms were identified. In addition, we provide the etymology of 46 vernacular names, which confirms that the vast majority of traditional Croatian cultivars have common and widespread names. The results presented herein underline the importance of safeguarding local cultivars and conducting continuous surveys.

The influence of different irrigation regimes on olive fruit morphological parameters and on the quantity and quality (marketable indices, phenolic content, fatty acid composition, and sensory profile) of virgin olive oil (VOO) obtained from the Croatian cultivar Oblica, grown on an extremely rocky and dry reclaimed karst soil, was studied over three years. Four treatments were applied: rain-fed and three treatments calculated as 50%, 75%, and 100% of the crop’s irrigation requirement (Irr). Principal component analysis separated growing seasons (GS) that differed in precipitation. In the 2016 season, which had a low number of fruits per kilogram and provided a higher amount of balanced VOO with medium to intense bitterness and pungency (rain-fed treatment), the oil yield increased by irrigation (Irr 75 and Irr 100) up to 18%, while unchanged phenolics, bitterness, and pungency were observed for the VOOs obtained. In the drier GS (2017), which under rain-fed conditions had high fruit per kg, smallest fruit sizes, and lowest oil yield, and in which the VOOs had high phenolic content and intense sensory taste attributes, fruit weight, fruit sizes, and oil yield increased by 35% in all irrigation treatments, while phenols, bitterness, and pungency decreased, balancing the sensory profile of the VOOs. The results obtained here led us to conclude that the irrigation of young olives resulted in a positive effect, with the indication that an abundant water supply is more effective in drought conditions.

Intercropping is a pivotal strategy for achieving Sustainable Development Goal (SDG) number 2—End hunger, achieve food security and improved nutrition and promote sustainable agriculture (SDG 2)—by enhancing food security agroecosystem resilience and sustainability. By integrating diverse species within the same plot, this sustainable approach takes advantage of the beneficial interactions between them. The simultaneous cultivation of multiple crop species within the same field increases agricultural diversification and contributes to a more resilient production system, breaking the uniformity of modern intensive agriculture. The objective of this review is to evaluate intercropping practices throughout the Mediterranean, specifically in Southern Europe (Portugal, Spain, Italy, and Greece), North Africa (Morocco, Algeria, and Tunisia), and the Middle East (Turkey, Israel, and Jordan). This review intends to show advantages and disadvantages of intercropping and crops used and also highlight how intercropping systems affect crop production and quality, soil quality and microbiome, and proliferation of weeds, pests and diseases. The literature suggests that diversification in agriculture supports biodiversity and ecosystem services by the cultivation of diverse crop species together and, hence, may reduce independence in external outputs such as nutrient supply, pesticides and soil amendment. Despite the potential benefits of intercropping, the major caveats of this practice are the competition between different crops on resources, potential risks of plant protection, technical challenges of integrating the different requirements of each crop used in the system, and culture-related restrictions or regulations.

The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) is considered one of the most important phytophagous pests worldwide. In Croatia, it severely affects the fresh fruit production in Dalmatia, and a national suppression action plan has been established to alleviate farmers’ burden. The aim of the current paper was to comprehensively study the overwintering dynamics of medfly in Dalmatia and investigate whether adults and pupae in addition to larvae can overwinter in different conditions, with a goal to support the establishment of a sound off-season control strategy against this pest. The off-season strategy, which was recently proposed in the frameworks of the EU Horizon 2020 funded project FF-IPM, focuses on the management of the low adult populations during winter and early spring period that has a detrimental effect on the development of the on-season summer and early autumn populations. Overwintering trials were conducted in Split (Dalmatia region, Croatia) using the local C. capitata population. Cages with different life stages (adults, pupae) or infested fruits were periodically transferred in three overwintering sites (open-field, semi-field, and urban conditions) from October to December (two to three establishment dates during the 2019–2020 and 2020–2021 season). Our results provide strong evidence that, in addition to larvae within fruit, both adult and pupae can survive in significant proportions in open-field and urban conditions. Overwintering site and establishment date were both significant predictors of the overwintering success of adults, and they affect pupae developmental duration, adult emergence rates from overwintering pupae, female fecundity, and generally the overwintering ability of the pupae. Our results suggest that the current national strategy for C. capitata suppression, which is based on the assumptions of larvae overwintering need to be adjusted by development and implementation of the off-season strategy.

Pistacia terebinthus L. ili smrdljika je listopadni grm ili nisko stablo iz porodice Anacardiaceae. Autohtona je vrsta Mediterana, a u Hrvatskoj je široko rasprostranjena od Istre, preko Hrvatskog primorja, Dalmacije, Dalmatinske zagore do krajnjeg juga. Kserofitna je vrsta, koja raste na osunčanim položajima u makijama i šikarama eumediteranske i submediteranske zone na suhim, toplim, kamenitim i stjenovitim mjestima. Na području prirodne rasprostranjenosti tradicionalno se koristila u prehrani, kao i u liječenju mnogih zdravstvenih tegoba, kao što su respiratorne i urinarne infekcije, želučane tegobe, reumatizam, itd. U novije vrijeme, analize kemijskog sastava vrste upućuju na znatan sadržaj mnogobrojnih aktivnih sastojaka visoke nutritivne vrijednosti i ljekovitog djelovanja (npr. eterična ulja, proteini, tanini, nezasićene masne kiseline, flavonoidi), a najpoznatija je po sadržaju smole iz koje se izdvaja terpentin. Zbog veće otpornosti na sušu, temperaturne ekstreme, bolesti i štetnike te s obzirom na rasprostranjenost na našem području, smrdljika ima najveći potencijal kao podloga za cijepljenje prave tršlje ili pistacije (P. vera L.), cijenjene zbog svojih plodova, što danas predstavlja upotrebu od najvećeg gospodarskog značaja, a prvi nasadi već se podižu u srednjoj Dalmaciji. Najčešći način cijepljenja je okuliranje koje se provodi na dobro razvijenim dvogodišnjim podlogama smrdljike. Izvjesni problem koji se javlja kod proizvodnje podloga je niska klijavost sjemena smrdljike zbog fiziološke i fizikalne dormantnosti, partenokarpije i abortiranja sjemenki. Dostupna znanstvena i stručna literatura kao i rezultati preliminarnih terenskih istraživanja upućuju na postojanje velikog potencijala upotrebe smrdljike u agronomiji, šumarstvu, farmaciji i prehrambenoj industriji.