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Drosophila suzukii is a polyphagous pest of small and soft fruit, originating from Asia, which has spread and established in Europe and the USA. Adults exhibit seasonal phenotypes, i.e., summer morphs (SM) and winter morphs (WM) to cope with fluctuating environmental conditions. WM have a darker cuticle and larger wings compared to SM, while WM females experience reproductive dormancy. We studied the life history traits (lifespan, female reproductive status and number of produced offspring) of WM and SM that were exposed to winter field conditions of a coastal and a mainland agricultural area, with mild and cold winter climates, respectively. Mated adults of each phenotype were individually placed in vials bearing nutritional/oviposition substrate, and transferred to the field from November 2019 to May 2020, when the death of the last individual was recorded. Almost all SM females (90%) and no WM female carried mature ovarioles before being transferred to the field. WM exhibited a longer lifespan than SM adjusting for location and sex. Differences in survival between the two phenotypes were more pronounced for adults kept in the mainland area. The majority of SM females produced offspring during overwintering in the mild coastal area, but only a few SM were reproductively active in the cold mainland area. Some WM females produced progeny during overwintering in the mild conditions of the coastal area, but all WM females were in reproductive arrest in the mainland area. Overwintering females in the coastal area had a shorter lifespan and produced more progeny than those kept in the mainland area. High survival rates of WM provide indications of the successful performance of this phenotype in the adverse conditions of the cold climates. Additionally, the continuous reproductive activity of SM females and the onset of progeny production by WM females during overwintering in the coastal area indicate that the insect remains reproductively active throughout the year in areas with mild climatic conditions. Our findings support the successful adaptation of D. suzukii in both areas tested and can be used for the development of area-specific population models, based on the prevailing climatic conditions.
Stella Papanastasiou; Vasilis Rodovitis; Eleni Verykouki; Evmorfia Bataka; Nikos Papadopoulos. Adaptation of an Invasive Pest to Novel Environments: Life History Traits of Drosophila suzukii in Coastal and Mainland Areas of Greece during Overwintering. Biology 2021, 10, 727 .
AMA StyleStella Papanastasiou, Vasilis Rodovitis, Eleni Verykouki, Evmorfia Bataka, Nikos Papadopoulos. Adaptation of an Invasive Pest to Novel Environments: Life History Traits of Drosophila suzukii in Coastal and Mainland Areas of Greece during Overwintering. Biology. 2021; 10 (8):727.
Chicago/Turabian StyleStella Papanastasiou; Vasilis Rodovitis; Eleni Verykouki; Evmorfia Bataka; Nikos Papadopoulos. 2021. "Adaptation of an Invasive Pest to Novel Environments: Life History Traits of Drosophila suzukii in Coastal and Mainland Areas of Greece during Overwintering." Biology 10, no. 8: 727.
FruiTemp is an open-source prototype developed to study the response of endophytic insect species such as fruit flies (Diptera: Tephrtidae) to variable temperature conditions including the controlled laboratory and fluctuating field settings. The system is a three-channel temperature sensor that consists of two precision thermistors that measure the temperature in the core and the surface of a fruit on a tree and a Harsh Environment thermistor that measures the air temperature surrounding the host at a rate of one measurement per 15 min. The sample rate can be adjusted according to the researcher’s needs. The system was successfully tested in field and laboratory experimental conditions using apples as the fruit model. The measurements on apples on trees lasted five consecutive days and produced a range of reliable data. After assessing statistical agreement and precision, the results revealed a differential bias of 0.331 °C and a proportional bias of a magnitude of 0.982. This work promotes open-source implementations allowing inexpensive solutions aiding experimentation procedures by significantly lowering operating costs.
Evmorfia Bataka; Vasilis Rodovitis; Kostas Zarpas; Nikos Papadopoulos; Christos Nakas. FruiTemp: Design, Implementation and Analysis for an Open-Source Temperature Logger Applied to Fruit Fly Host Experimentation. Applied Sciences 2021, 11, 6003 .
AMA StyleEvmorfia Bataka, Vasilis Rodovitis, Kostas Zarpas, Nikos Papadopoulos, Christos Nakas. FruiTemp: Design, Implementation and Analysis for an Open-Source Temperature Logger Applied to Fruit Fly Host Experimentation. Applied Sciences. 2021; 11 (13):6003.
Chicago/Turabian StyleEvmorfia Bataka; Vasilis Rodovitis; Kostas Zarpas; Nikos Papadopoulos; Christos Nakas. 2021. "FruiTemp: Design, Implementation and Analysis for an Open-Source Temperature Logger Applied to Fruit Fly Host Experimentation." Applied Sciences 11, no. 13: 6003.
The Culex pipiens (Diptera: Culicidae) mosquito is of high medical importance as it is considered the prime vector of West Nile virus. In Europe, this species consists of two forms, named pipiens and molestus, that exhibit substantial differences in their overwintering biology. Diflubenzuron (DFB) and Bacillus thuringiensis var. israelensis (Bti) are two of the most used larvicides in mosquito control, including that of Culex pipiens. The high dependency on these two larvicides poses major concerns for resistance development. The evolution and stability of resistance to insecticides has been associated with fitness costs that may be manifested under stressful conditions, such as the winter period. This study investigated how short-term selection of pipiens and molestus forms to both larvicides affect their overwintering success. Larvae from each form were subjected to the same selective pressure (80% mortality) for three successive generations with DFB and Bti. At the end of this process, the winter survival between the selected populations and the controls (colonies without selection) was determined for each form. Selection to both larvicides significantly reduced the winter survival rates of molestus but not of pipiens form, indicating potential differences in the persistence of the selected individuals from year to year between the two forms.
Charalampos Ioannou; Christos Hadjichristodoulou; Maria Kyritsi; Nikos Papadopoulos. Short-Term Selection to Diflubenzuron and Bacillus thuringiensis Var. Israelensis Differentially Affects the Winter Survival of Culex pipiens f. Pipiens and Culex pipiens f. Molestus (Diptera: Culicidae). Insects 2021, 12, 527 .
AMA StyleCharalampos Ioannou, Christos Hadjichristodoulou, Maria Kyritsi, Nikos Papadopoulos. Short-Term Selection to Diflubenzuron and Bacillus thuringiensis Var. Israelensis Differentially Affects the Winter Survival of Culex pipiens f. Pipiens and Culex pipiens f. Molestus (Diptera: Culicidae). Insects. 2021; 12 (6):527.
Chicago/Turabian StyleCharalampos Ioannou; Christos Hadjichristodoulou; Maria Kyritsi; Nikos Papadopoulos. 2021. "Short-Term Selection to Diflubenzuron and Bacillus thuringiensis Var. Israelensis Differentially Affects the Winter Survival of Culex pipiens f. Pipiens and Culex pipiens f. Molestus (Diptera: Culicidae)." Insects 12, no. 6: 527.
Mass trapping is an environmentally safe alternative to insecticide application for the Mediterranean fruit fly management. The selection of effective trap-attractant combinations for monitoring and mass trapping control remains challenging. The current study explored the attractiveness of trapping devices during spring (early season) and summer (late season) in field cage trials. Five trapping devices were assessed: (a) the commercially available Decis® trap, (b) Tephri trap baited with Biodelear, (c) Tephri trap baited with BioLure, (d) International Pheromone McPhail trap (IPMT) baited with Biodelear, and (e) IPMT baited with BioLure. On a test day, 100 adults (50 males and 50 females) were released in each field cage wherein traps were placed individually. Trap captures were recorded at hourly intervals from 10:30 am to 5:30 pm. Our results showed that Tephri traps baited either with BioLure or Biodelear captured the most adults under low temperatures. Efficacy of Tephri traps baited with BioLure were higher than that of other trap-attractant combinations at high temperatures. Adult captures in Decis® trap were low during both seasons. More males than females were captured at low temperatures. Both efficacy and female selectivity of trapping devices are related to prevailing temperature regimes during spring and summer under semi-field conditions.
Eleftheria-Maria Bali; Cleopatra Moraiti; Charalampos Ioannou; Vasilis Mavraganis; Nikos Papadopoulos. Evaluation of Mass Trapping Devices for Early Seasonal Management of Ceratitis Capitata (Diptera: Tephritidae) Populations. Agronomy 2021, 11, 1101 .
AMA StyleEleftheria-Maria Bali, Cleopatra Moraiti, Charalampos Ioannou, Vasilis Mavraganis, Nikos Papadopoulos. Evaluation of Mass Trapping Devices for Early Seasonal Management of Ceratitis Capitata (Diptera: Tephritidae) Populations. Agronomy. 2021; 11 (6):1101.
Chicago/Turabian StyleEleftheria-Maria Bali; Cleopatra Moraiti; Charalampos Ioannou; Vasilis Mavraganis; Nikos Papadopoulos. 2021. "Evaluation of Mass Trapping Devices for Early Seasonal Management of Ceratitis Capitata (Diptera: Tephritidae) Populations." Agronomy 11, no. 6: 1101.
The Dipteran family Tephritidae (true fruit flies) comprises more than 5000 species classified in 500 genera distributed worldwide. Tephritidae include devastating agricultural pests and highly invasive species whose spread is currently facilitated by globalization, international trade and human mobility. The ability to identify and exploit a wide range of host plants for oviposition, as well as effective and diversified reproductive strategies, are among the key features supporting tephritid biological success. Intraspecific communication involves the exchange of a complex set of sensory cues that are species- and sex-specific. Chemical signals, which are standing out in tephritid communication, comprise long-distance pheromones emitted by one or both sexes, cuticular hydrocarbons with limited volatility deposited on the surrounding substrate or on the insect body regulating medium- to short-distance communication, and host-marking compounds deposited on the fruit after oviposition. In this review, the current knowledge on tephritid chemical communication was analysed with a special emphasis on fruit fly pest species belonging to the Anastrepha, Bactrocera,Ceratitis, Rhagoletis and Zeugodacus genera. The multidisciplinary approaches adopted for characterising tephritid semiochemicals, and the real-world applications and challenges for Integrated Pest Management (IPM) and biological control strategies are critically discussed. Future perspectives for targeted research on fruit fly chemical communication are highlighted.
Francesca Scolari; Federica Valerio; Giovanni Benelli; Nikos Papadopoulos; Lucie Vaníčková. Tephritid Fruit Fly Semiochemicals: Current Knowledge and Future Perspectives. Insects 2021, 12, 408 .
AMA StyleFrancesca Scolari, Federica Valerio, Giovanni Benelli, Nikos Papadopoulos, Lucie Vaníčková. Tephritid Fruit Fly Semiochemicals: Current Knowledge and Future Perspectives. Insects. 2021; 12 (5):408.
Chicago/Turabian StyleFrancesca Scolari; Federica Valerio; Giovanni Benelli; Nikos Papadopoulos; Lucie Vaníčková. 2021. "Tephritid Fruit Fly Semiochemicals: Current Knowledge and Future Perspectives." Insects 12, no. 5: 408.
The recent spread of invasive mosquito species, such as Aedes albopictus and the seasonal sporadic transmission of autochthonous cases of arboviral diseases (e.g., dengue, chikungunya, Zika) in temperate areas, such as Europe and North America, highlight the importance of effective mosquito-control interventions to reduce not only nuisance, but also major threats for public health. Local, regional, and even national mosquito control programs have been established in many countries and are executed on a seasonal basis by either public or private bodies. In order for these interventions to be worthwhile, funding authorities should ensure that mosquito control is (a) planned by competent scientific institutions addressing the local demands, (b) executed following the plan that is based on recommended and effective methods and strategies, (c) monitored regularly by checking the efficacy of the implemented actions, (d) evaluated against the set of targets, and (e) regularly improved according to the results of the monitoring. Adherence to these conditions can only be assured if a formal quality management system is adopted and enforced that ensures the transparency of effectiveness of the control operation. The current paper aims at defining the two components of this quality management system, quality assurance and quality control for mosquito control programs with special emphasis on Europe, but applicable over temperate areas.
Antonios Michaelakis; Fabrizio Balestrino; Norbert Becker; Romeo Bellini; Beniamino Caputo; Alessandra della Torre; Jordi Figuerola; Gregory L’Ambert; Dusan Petric; Vincent Robert; David Roiz; Anastasios Saratsis; Carla Sousa; William Wint; Nikos Papadopoulos. A Case for Systematic Quality Management in Mosquito Control Programmes in Europe. International Journal of Environmental Research and Public Health 2021, 18, 3478 .
AMA StyleAntonios Michaelakis, Fabrizio Balestrino, Norbert Becker, Romeo Bellini, Beniamino Caputo, Alessandra della Torre, Jordi Figuerola, Gregory L’Ambert, Dusan Petric, Vincent Robert, David Roiz, Anastasios Saratsis, Carla Sousa, William Wint, Nikos Papadopoulos. A Case for Systematic Quality Management in Mosquito Control Programmes in Europe. International Journal of Environmental Research and Public Health. 2021; 18 (7):3478.
Chicago/Turabian StyleAntonios Michaelakis; Fabrizio Balestrino; Norbert Becker; Romeo Bellini; Beniamino Caputo; Alessandra della Torre; Jordi Figuerola; Gregory L’Ambert; Dusan Petric; Vincent Robert; David Roiz; Anastasios Saratsis; Carla Sousa; William Wint; Nikos Papadopoulos. 2021. "A Case for Systematic Quality Management in Mosquito Control Programmes in Europe." International Journal of Environmental Research and Public Health 18, no. 7: 3478.
Aedes albopictus, an invasive mosquito species, is currently well established in many European countries, posing high risks to human health. A preliminary trial using repetitive releases of irradiated sterile male mosquitoes was designed, implemented and evaluated for the first time in Greece. The main scope of this trial was to investigate the efficacy of sterile insect technique (SIT) on wild population egg hatch rate in Greece using mass-produced sterile male mosquitoes from another country (Italy). The study was conducted in Vravrona area, close to Athens International Airport (Attica Region). The number of eggs laid in ovitraps was similar in all intervention and control plots. However, a significant reduction in egg hatch rate was recorded in the SIT plot in comparison with both control plots starting two weeks after the first release. This trial validates the logistics (transportation, releases handling and monitoring) as a major step towards implementing efficient, environmentally safe control approaches as an additional tool against the invasive Aedes species in Greece and more widely in Europe.
Georgios Balatsos; Arianna Puggioli; Vasileios Karras; Ioanna Lytra; George Mastronikolos; Marco Carrieri; Dimitrios Papachristos; Marco Malfacini; Angeliki Stefopoulou; Charalampos Ioannou; Fabrizio Balestrino; Jérémy Bouyer; Dušan Petrić; Igor Pajović; Apostolos Kapranas; Nikos Papadopoulos; Panagiotis Milonas; Romeo Bellini; Antonios Michaelakis. Reduction in Egg Fertility of Aedes albopictus Mosquitoes in Greece Following Releases of Imported Sterile Males. Insects 2021, 12, 110 .
AMA StyleGeorgios Balatsos, Arianna Puggioli, Vasileios Karras, Ioanna Lytra, George Mastronikolos, Marco Carrieri, Dimitrios Papachristos, Marco Malfacini, Angeliki Stefopoulou, Charalampos Ioannou, Fabrizio Balestrino, Jérémy Bouyer, Dušan Petrić, Igor Pajović, Apostolos Kapranas, Nikos Papadopoulos, Panagiotis Milonas, Romeo Bellini, Antonios Michaelakis. Reduction in Egg Fertility of Aedes albopictus Mosquitoes in Greece Following Releases of Imported Sterile Males. Insects. 2021; 12 (2):110.
Chicago/Turabian StyleGeorgios Balatsos; Arianna Puggioli; Vasileios Karras; Ioanna Lytra; George Mastronikolos; Marco Carrieri; Dimitrios Papachristos; Marco Malfacini; Angeliki Stefopoulou; Charalampos Ioannou; Fabrizio Balestrino; Jérémy Bouyer; Dušan Petrić; Igor Pajović; Apostolos Kapranas; Nikos Papadopoulos; Panagiotis Milonas; Romeo Bellini; Antonios Michaelakis. 2021. "Reduction in Egg Fertility of Aedes albopictus Mosquitoes in Greece Following Releases of Imported Sterile Males." Insects 12, no. 2: 110.
Despite the recent invasion and wide spread of Drosophila suzukii Matsumura (Diptera: Drosophilidae) in Europe, little is known regarding its population trends in coastal areas of the southern Mediterranean countries. Using adult trapping and fruit sampling, we studied the population dynamics of D. suzukii in coastal and mainland (semi-highland) cherry orchards of Greece, from 2018 to 2020. Adults were captured in traps baited with apple cider vinegar, placed in conventional and unmanaged sweet-cherry orchards, and in neighbouring wild growing hosts. Sampling of sweet-cherry fruit to assess infestation levels was conducted from early and late-ripening cherry cultivars in both areas. Adults were captured throughout the year in the coastal area with two peaks registered in spring and late-autumn. Captures were nearly zero during the hot summer months. Flight activity exhibited only one peak in autumn at the mainland area, and ceased during winter and spring. Captures in wild hosts were lower during the sweet-cherry ripening period than later in the season. Higher sweet-cherry infestation levels were recorded in the coastal than in the mainland area and in unmanaged than in commercial orchards. Both early and late-ripening cultivars were highly susceptible to D. suzukii infestation in the coastal area. Infestation rates were higher in late-ripening cultivars than in early-ripening ones in the mainland area. We conclude that D. suzukii has well adapted to the Mediterranean climate of Greece, and is able to progressively exploit several crops and wild hosts of mainland and coastal areas.
Stella A. Papanastasiou; Vasilis G. Rodovitis; Evmorfia P. Bataka; Eleni Verykouki; Nikos T. Papadopoulos. Population Dynamics of Drosophila suzukii in Coastal and Mainland Sweet Cherry Orchards of Greece. Insects 2020, 11, 621 .
AMA StyleStella A. Papanastasiou, Vasilis G. Rodovitis, Evmorfia P. Bataka, Eleni Verykouki, Nikos T. Papadopoulos. Population Dynamics of Drosophila suzukii in Coastal and Mainland Sweet Cherry Orchards of Greece. Insects. 2020; 11 (9):621.
Chicago/Turabian StyleStella A. Papanastasiou; Vasilis G. Rodovitis; Evmorfia P. Bataka; Eleni Verykouki; Nikos T. Papadopoulos. 2020. "Population Dynamics of Drosophila suzukii in Coastal and Mainland Sweet Cherry Orchards of Greece." Insects 11, no. 9: 621.
BACKGROUND Linalool is a natural scent, found in essential oils (EOs) of several plants. It is widely used as a fragrant, also possessing toxic, oviposition deterrent, and repellent properties against many insect species. Ceratitis capitata (medfly) is one of the most notorious insect pests for international fresh fruit production. Despite the available information on the repellent effects of EOs on herbivorous insects, possible ovipositional deterrent effects of linalool against C. capitata remain unknown. OBJECTIVES AND RESULTS We tested whether different linalool concentrations, application methods on fruit hosts and time since application elicited oviposition‐deterrence on medfly, from bitter oranges, apples and nectarines, under constant laboratory conditions. We also tested possible repellent properties of linalool on adult medflies in field conditions. Females drilled less oviposition stings and deposited fewer eggs per fruit in all linalool‐treated hosts. Spraying and topical‐droplet application were the most efficient methods on bitter oranges, although spraying was the least effective one in apples. Bitter oranges and nectarines that were offered to females immediately after exposure to linalool received more oviposition stings and eggs than those offered three days post‐exposure to linalool solutions. Conversely, apples tested three days following linalool application received significantly more oviposition stings and eggs than those tested immediately and one day post exposure. More adults in total and more females were captured in traps located on untreated‐control than on linalool treated trees, especially in citrus hosts. CONCLUSION Linalool has a potential future use in environmental friendly control strategies against C. capitata. This article is protected by copyright. All rights reserved.
Stella A. Papanastasiou; Charalampos S. Ioannou; Nikos T. Papadopoulos. Oviposition‐deterrent effect of linalool – a compound of citrus essential oils – on female Mediterranean fruit flies, Ceratitis capitata ( Diptera: Tephritidae ). Pest Management Science 2020, 76, 3066 -3077.
AMA StyleStella A. Papanastasiou, Charalampos S. Ioannou, Nikos T. Papadopoulos. Oviposition‐deterrent effect of linalool – a compound of citrus essential oils – on female Mediterranean fruit flies, Ceratitis capitata ( Diptera: Tephritidae ). Pest Management Science. 2020; 76 (9):3066-3077.
Chicago/Turabian StyleStella A. Papanastasiou; Charalampos S. Ioannou; Nikos T. Papadopoulos. 2020. "Oviposition‐deterrent effect of linalool – a compound of citrus essential oils – on female Mediterranean fruit flies, Ceratitis capitata ( Diptera: Tephritidae )." Pest Management Science 76, no. 9: 3066-3077.
The Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae), holds an impressive record of successful invasions promoted by the growth and development of international fruit trade. Hence, survival of immatures within infested fruit that are subjected to various conditions during transportation seems to be a crucial feature that promotes invasion success. Wolbachia pipientis is a common endosymbiont of insects and other arthropods generating several biological effects on its hosts. Existing information report the influence of Wolbachia on the fitness traits of insect host species, including the Mediterranean fruit fly. However, little is known regarding effects of Wolbachia infection on immature development in different host fruits and temperatures. This study was conducted to determine the development and survival of immature stages of four different Mediterranean fruit fly populations, either infected or uninfected with Wolbachia, in two hosts (apples, bitter oranges) under three constant temperatures (15, 25 and 30°C), constant relative humidity (45–55 ± 5%), and a photoperiod of 14L:10D. Our findings demonstrate both differential response of two fruit fly lines to Wolbachia infection and differential effects of the two Wolbachia strains on the same Mediterranean fruit fly line. Larva-to-pupa and larva-to-adult survival followed similar patterns and varied a lot among the four medfly populations, the two host fruits and the different temperatures. Pupation rates and larval developmental time were higher for larvae implanted in apples compared to bitter oranges. The survival rates of wildish medflies were higher than those of the laboratory adapted ones, particularly in bitter oranges. The Wolbachia infected medflies, expressed lower survival rates and higher developmental times, especially the wCer4 infected line. High temperatures constrained immature development and were lethal for the Wolbachia infected wCer4 medfly line. Lower temperatures inferred longer developmental times to immature stages of all medfly populations tested, in both host fruits. Implications on the ecology and survival of the fly in nature are discussed.
Niki K. Dionysopoulou; Stella A. Papanastasiou; Georgios A. Kyritsis; Nikos T. Papadopoulos. Effect of host fruit, temperature and Wolbachia infection on survival and development of Ceratitis capitata immature stages. PLOS ONE 2020, 15, e0229727 .
AMA StyleNiki K. Dionysopoulou, Stella A. Papanastasiou, Georgios A. Kyritsis, Nikos T. Papadopoulos. Effect of host fruit, temperature and Wolbachia infection on survival and development of Ceratitis capitata immature stages. PLOS ONE. 2020; 15 (3):e0229727.
Chicago/Turabian StyleNiki K. Dionysopoulou; Stella A. Papanastasiou; Georgios A. Kyritsis; Nikos T. Papadopoulos. 2020. "Effect of host fruit, temperature and Wolbachia infection on survival and development of Ceratitis capitata immature stages." PLOS ONE 15, no. 3: e0229727.
The European cherry fruit fly, Rhagoletis cerasi (Diptera: Tephritidae), is a univoltine species that undergoes obligatory summer–winter diapause at pupal stage in the soil (2–5 cm) beneath host trees. To study the effects of photoperiod and relative humidity on diapause termination and post-winter developmental duration of R. cerasi, pupae collected from Dossenheim (Germany) were exposed to different photoperiod or relative humidity regimes during a chilling period ranging from 2 to 8.5 months. Specifically, pupae were exposed to four photoperiod regimes: (a) light conditions (24L:00D), (b) dark conditions (00L:24D), (c) short photoperiod (08L:16D) and (d) long photoperiod (16L:08D), as well as to three relative humidity regimes: (a) low (40% RH), (b) medium (60% RH) and (c) high (70–80% RH). Data revealed that relative humidity is not a significant predictor of diapause termination, but it affects the post-winter developmental period. Higher relative humidity promotes post-winter pupae development. On the other hand, photoperiod significantly affected both diapause termination and post-winter development of R. cerasi pupae. Light conditions (24L:00D) accelerate adult emergence, particularly for females. Regardless of the photoperiod (24L:00D, 00L:24D, 08L:16D), rates of adult emergence were high (>75%) for chilling intervals longer than 6.5 months. Nonetheless, exposure to a long day photoperiod (16L:08D), during chilling, dramatically reduced the proportion of adult emergence following 6 months exposure to chilling. Our findings broaden the understanding of factors regulating diapause responses in European cherry fruit fly, local adaptation and synchronization of adult emergence with the ripening period of major hosts.
Cleopatra A. Moraiti; Kirsten Köppler; Heidrun Vogt; Nikos T. Papadopoulos. Effects of photoperiod and relative humidity on diapause termination and post-winter development of Rhagoletis cerasi pupae. Bulletin of Entomological Research 2020, 110, 588 -596.
AMA StyleCleopatra A. Moraiti, Kirsten Köppler, Heidrun Vogt, Nikos T. Papadopoulos. Effects of photoperiod and relative humidity on diapause termination and post-winter development of Rhagoletis cerasi pupae. Bulletin of Entomological Research. 2020; 110 (5):588-596.
Chicago/Turabian StyleCleopatra A. Moraiti; Kirsten Köppler; Heidrun Vogt; Nikos T. Papadopoulos. 2020. "Effects of photoperiod and relative humidity on diapause termination and post-winter development of Rhagoletis cerasi pupae." Bulletin of Entomological Research 110, no. 5: 588-596.
Background Wolbachia pipientisis a widespread, obligatory intracellular and maternally inherited bacterium, that induces a wide range of reproductive alterations to its hosts. Cytoplasmic Incompatibility (CI) is causing embryonic lethality, the most common of them. Despite thatWolbachia-borne sterility has been proposed as an environmental friendly pest control method (Incompatible Insect Technique, IIT) since 1970s, the fact thatWolbachiamodifies important fitness components of its hosts sets severe barriers to IIT implementation. Mass rearing of Mediterranean fruit fly,Ceratitis capitata(medfly), is highly optimized given that this pest is a model species regarding the implementation of another sterility based pest control method, the Sterile Insect Technique (SIT). We used the medfly-Wolbachiasymbiotic association, as a model system, to study the effect of two differentWolbachiastrains, on the life history traits of 2C. capitatalines with different genomic background. Results Wolbachiaeffects are regulated by bothC. capitatagenetic background and theWolbachiastrain.Wolbachiainfection reduces fertility rates in bothC. capitatagenetic backgrounds and shortens the pre-pupa developmental duration in the GSS strain. On the other hand, regardless of the strain ofWolbachia(wCer2,wCer4) infection does not affect either the sex ratio or the longevity of adults.wCer4 infection imposed a reduction in females’ fecundity butwCer2 did not. Male mating competitiveness, adults flight ability and longevity under water and food deprivation were affected by both the genetic background of medfly and the strain ofWolbachia(genotype by genotype interaction). Conclusion Wolbachiainfection could alter important life history traits of mass-rearedC. capitatalines and therefore the response of each genotype on theWolbachiainfection should be considered toward ensuring the productivity of theWolbachia-infected insects under mass-rearing conditions.
Georgios A. Kyritsis; Antonios A. Augustinos; Ioannis Livadaras; Carlos Cáceres; Kostas Bourtzis; Nikos T. Papadopoulos. Medfly-Wolbachia symbiosis: genotype x genotype interactions determine host’s life history traits under mass rearing conditions. BMC Biotechnology 2019, 19, 1 -15.
AMA StyleGeorgios A. Kyritsis, Antonios A. Augustinos, Ioannis Livadaras, Carlos Cáceres, Kostas Bourtzis, Nikos T. Papadopoulos. Medfly-Wolbachia symbiosis: genotype x genotype interactions determine host’s life history traits under mass rearing conditions. BMC Biotechnology. 2019; 19 (2):1-15.
Chicago/Turabian StyleGeorgios A. Kyritsis; Antonios A. Augustinos; Ioannis Livadaras; Carlos Cáceres; Kostas Bourtzis; Nikos T. Papadopoulos. 2019. "Medfly-Wolbachia symbiosis: genotype x genotype interactions determine host’s life history traits under mass rearing conditions." BMC Biotechnology 19, no. 2: 1-15.
Response of endophytic fruit fly species (Tephritidae) to larval crowding is a form of scramble competition that may affect important life history traits of adults, such as survival and reproduction. Recent empirical evidence demonstrates large differences in adult life history traits, especially longevity, among Mediterranean fruit fly (Ceratitis capitata; "medfly") biotypes obtained from different regions of the world. However, whether the evolution of long lifespan is associated with response to stress induced by larval crowding has not been fully elucidated. We investigated, under constant laboratory conditions, the response of a short‐ and a long‐lived medfly biotypes to stress induced by larval crowding. Survival and development of larvae and pupae and the size of resulting pupae were recorded. The lifespan and age‐specific egg production patterns of the obtained adults were recorded. Our findings reveal that increased larval density reduced immature survival (larvae and pupae) in the short‐lived biotype but had rather neutral effects on the longed‐lived one. Only larvae of the long‐lived biotype were capable of prolonging their developmental duration under the highest crowding regime to successfully pupate and emerge as adults. Response of emerging adults to larvae crowding conditions was similar in the two medfly biotypes. Those individuals emerging from high larval density regimes had reduced longevity and fecundity. Long‐lived biotype individuals, however, appeared to suffer a higher cost in longevity compared with the short‐lived one. The importance of our findings to understand the evolution of long lifespan is discussed.
Alexandros D. Diamantidis; Charalampos S. Ioannou; Christos Nakas; James R. Carey; Nikos T. Papadopoulos. Differential response to larval crowding of a long‐ and a short‐lived medfly biotype. Journal of Evolutionary Biology 2019, 33, 329 -341.
AMA StyleAlexandros D. Diamantidis, Charalampos S. Ioannou, Christos Nakas, James R. Carey, Nikos T. Papadopoulos. Differential response to larval crowding of a long‐ and a short‐lived medfly biotype. Journal of Evolutionary Biology. 2019; 33 (3):329-341.
Chicago/Turabian StyleAlexandros D. Diamantidis; Charalampos S. Ioannou; Christos Nakas; James R. Carey; Nikos T. Papadopoulos. 2019. "Differential response to larval crowding of a long‐ and a short‐lived medfly biotype." Journal of Evolutionary Biology 33, no. 3: 329-341.
Modern agriculture requires technology to give precise measures about relevant parameters such as pest control. Here, we developed a decision support system (DSS) based on semi-automatic pest monitoring for managing the olive fruit fly Bactrocera oleae (Rossi), in Mallorca (Balearic Islands, Spain). The DSS was based on an algorithm that took into account spatial and temporal patterns of olive fruit fly population in an orchard where all trees were georeferenced, thus precise treatments against the pest were conducted through a location aware system (LAS). The olive fruit fly adult population was monitored by using ad hoc off-the-grid autonomous electronic traps.The results were compared with those obtained with conventional methods. For a pilot trial, we selected an olive-producing orchard, where from June to October 2015, three plots using LAS management and three plots under conventional control (NO-LAS plots) were compared. Spray threshold considered both adult population and fruit damage. An additional non-sprayed plot was selected for assessing biological control due to the parasitoid, Psyttalia concolor (Szépligeti). Results showed that the use of DSS reduced by 36.84% the volume of insecticide used in LAS compared to NO-LAS plots. Accordingly, time and distance needed for spraying were also reduced. Adult olive fruit fly population was lower in the LAS plots when compared with the NO-LAS plots; conversely, fruit infestation was higher in LAS compared with NO-LAS. The implementation of LAS and DSS at field level allowed real-time monitoring of adult olive flies, thereby increasing the accuracy and precision of sprays in time and space and decreasing impact on natural enemies.
Miguel Ángel Miranda; Carlos Barceló; Ferran Valdés; José Francisco Feliu; David Nestel; Nikolaos Papadopoulos; Andrea Sciarretta; Maurici Ruiz; Bartomeu Alorda. Developing and Implementation of Decision Support System (DSS) for the Control of Olive Fruit Fly, Bactrocera Oleae, in Mediterranean Olive Orchards. Agronomy 2019, 9, 620 .
AMA StyleMiguel Ángel Miranda, Carlos Barceló, Ferran Valdés, José Francisco Feliu, David Nestel, Nikolaos Papadopoulos, Andrea Sciarretta, Maurici Ruiz, Bartomeu Alorda. Developing and Implementation of Decision Support System (DSS) for the Control of Olive Fruit Fly, Bactrocera Oleae, in Mediterranean Olive Orchards. Agronomy. 2019; 9 (10):620.
Chicago/Turabian StyleMiguel Ángel Miranda; Carlos Barceló; Ferran Valdés; José Francisco Feliu; David Nestel; Nikolaos Papadopoulos; Andrea Sciarretta; Maurici Ruiz; Bartomeu Alorda. 2019. "Developing and Implementation of Decision Support System (DSS) for the Control of Olive Fruit Fly, Bactrocera Oleae, in Mediterranean Olive Orchards." Agronomy 9, no. 10: 620.
A Decision Support System (DSS) was developed and evaluated to control the Mediterranean fruit fly (medfly), Ceratitis capitata (Wiedermann), by incorporating a semi-automatic pest monitoring and a precision targeting approach in multi-varietal orchards. The DSS consists of three algorithms. DSS1, based on the degree days calculation, defines when the traps should be deployed in the field initiating the medfly population monitoring. DSS2 defines the areas to be treated and the type of treatment based on the number of adult medfly captures, harvesting time, and phenological stage of the host cultivar. DSS3 defines the spraying procedure considering the technical registration properties of the selected insecticide (e.g., withholding period and efficacy duration time) and weather conditions. The DSS was tested in commercial orchard conditions near Rome, central Italy, with a randomized complete blocks experimental design, comparing DSS-assisted and conventional management. In the DSS-assisted plots, a semi-automatic adult medfly monitoring system was deployed, composed of real-time, wireless electronic traps. The output of the functioning DSS is a map of spraying recommendation, reporting the areas to be treated and the treatment type (bait or cover insecticide spraying). The farmer was left free to follow, or not, the DSS indications. The first medfly captures were observed on June 30, whereas the DD threshold was reached on July 3 when the DSS started to operate. The field test produced 29 DSS decisions from July 3 to September 1 and confirmed that medfly management using the DSS substantially reduced the number of pesticide applications, the treated area, and the volumes of pesticide utilization. No significant differences in infested fruit were observed between DSS-assisted and conventional management. The level of acceptance of the DSS by the farmer was 78%. This evidence confirmed the requirement of fully involving farmers and pest managers during the evaluation process of DSS.
Andrea Sciarretta; Maria Rosaria Tabilio; Armando Amore; Marco Colacci; Miguel Á. Miranda; David Nestel; Nikos T. Papadopoulos; Pasquale Trematerra. Defining and Evaluating a Decision Support System (DSS) for the Precise Pest Management of the Mediterranean Fruit Fly, Ceratitis capitata, at the Farm Level. Agronomy 2019, 9, 608 .
AMA StyleAndrea Sciarretta, Maria Rosaria Tabilio, Armando Amore, Marco Colacci, Miguel Á. Miranda, David Nestel, Nikos T. Papadopoulos, Pasquale Trematerra. Defining and Evaluating a Decision Support System (DSS) for the Precise Pest Management of the Mediterranean Fruit Fly, Ceratitis capitata, at the Farm Level. Agronomy. 2019; 9 (10):608.
Chicago/Turabian StyleAndrea Sciarretta; Maria Rosaria Tabilio; Armando Amore; Marco Colacci; Miguel Á. Miranda; David Nestel; Nikos T. Papadopoulos; Pasquale Trematerra. 2019. "Defining and Evaluating a Decision Support System (DSS) for the Precise Pest Management of the Mediterranean Fruit Fly, Ceratitis capitata, at the Farm Level." Agronomy 9, no. 10: 608.
The European cherry fruit fly, Rhagoletis cerasi (Diptera: Tephritidae), is a key pest for the cherry production industry in Europe and west Asia that has recently invaded North America. Insecticide applications are frequently employed to control this devastating pest, often without considering its population trends. We developed a novel decision support system (DSS), and field tested it in commercial sweet cherry orchards in central Greece. The DSS includes two algorithms that predict the timing of adult activity in the wild and support pest management decisions, based on R. cerasi population trends and pesticide properties, respectively. Preparatory monitoring of the testing area during 2014, using adult traps, revealed high population densities of R. cerasi in non-managed sweet cherry orchards and low densities in commercial ones. Implementation of the DSS during 2015 resulted in low R. cerasi adult population densities and zero fruit infestation rates in commercial cherry orchards. Similar population and infestation rates were recorded in conventionally treated plots that received on average two insecticide applications compared to the one-half that the DSS treated plots received. Simultaneously, high population densities and fruit infestation rates were recorded in non-managed cherry orchards. Apparently, the implementation of the simple DSS we developed reduces the cost of R. cerasi management and minimizes the chemical footprint on both the harvested fruit and the environment.
Charalampos S. Ioannou; Stella A. Papanastasiou; Kostas D. Zarpas; Miguel Angel Miranda; Andrea Sciarretta; David Nestel; Nikos T. Papadopoulos. Development and Field Testing of a Spatial Decision Support System to Control Populations of the European Cherry Fruit Fly, Rhagoletis cerasi, in Commercial Orchards. Agronomy 2019, 9, 568 .
AMA StyleCharalampos S. Ioannou, Stella A. Papanastasiou, Kostas D. Zarpas, Miguel Angel Miranda, Andrea Sciarretta, David Nestel, Nikos T. Papadopoulos. Development and Field Testing of a Spatial Decision Support System to Control Populations of the European Cherry Fruit Fly, Rhagoletis cerasi, in Commercial Orchards. Agronomy. 2019; 9 (10):568.
Chicago/Turabian StyleCharalampos S. Ioannou; Stella A. Papanastasiou; Kostas D. Zarpas; Miguel Angel Miranda; Andrea Sciarretta; David Nestel; Nikos T. Papadopoulos. 2019. "Development and Field Testing of a Spatial Decision Support System to Control Populations of the European Cherry Fruit Fly, Rhagoletis cerasi, in Commercial Orchards." Agronomy 9, no. 10: 568.
Using a well-established model species for demographic, behavioural and aging research, the Mediterranean fruit fly (Ceratitis capitata), we explored whether nutritional stress early in adult life affects the sexual performance and survival in older ages. To do so we established two different protein starvation (PS) protocols that included the elimination of proteinaceous diet either before or after sexual maturity of male medflies. The frequency of sexual signalling and the age of death were daily recorded. Sexual signalling is directly related with male mating success in this model system. PS early in adult life results in high mortality rates (similar to sugar-only fed males), which are gradually restored in more advanced ages. Provision of a proteinaceous diet following early-life PS increases straightaway male sexual signalling to levels similar with those having continuous access to proteinaceous diet. Switching diet regimes from a protein-free to a protein-rich one progressively compensates mortality rates. Apparently, males prioritize sexual signalling over lifespan. PS after attaining sexual maturity significantly reduces both longevity and sexual performance. Access to protein only early in life is insufficient to support lifetime energy-consuming behaviours such as sexual signalling. Continuous access to a proteinaceous diet determines both lifetime sexual performance and longevity. Early in life PS males prioritize the allocation of nutritional elements, when available, in sexual activities over soma-maintenance.
Stella Papanastasiou; James R. Carey; Nikos T. Papadopoulos. Effects of early-life protein starvation on longevity and sexual performance of male medfly. PLOS ONE 2019, 14, e0219518 .
AMA StyleStella Papanastasiou, James R. Carey, Nikos T. Papadopoulos. Effects of early-life protein starvation on longevity and sexual performance of male medfly. PLOS ONE. 2019; 14 (7):e0219518.
Chicago/Turabian StyleStella Papanastasiou; James R. Carey; Nikos T. Papadopoulos. 2019. "Effects of early-life protein starvation on longevity and sexual performance of male medfly." PLOS ONE 14, no. 7: e0219518.
The genus Rhagoletis (Diptera: Tephritidae) comprises more than 65 species distributed throughout Europe, Asia and America, including many species of high economic importance. Currently, there are three Rhagoletis species that infest fruits and nuts in Europe. The European cherry fruit fly, Rhagoletis cerasi (may have invaded Europe a long time ago from the Caucasian area of West Asia), and two invasive species (recently introduced from North America): the eastern American cherry fruit fly, R. cingulata, and the walnut husk fly, R. completa. The presence of different Rhagoletis species may enhance population dynamics and establish an unpredictable economic risk for several fruit and nut crops in Europe. Despite their excessive economic importance, little is known on population dynamics, genetics and symbiotic associations for making sound pest control decisions in terms of species-specific, environmental friendly pest control methods. To this end, the current paper (a) summarizes recently accumulated genetic and population data for the European Rhagoletis species and their association with the endosymbiont Wolbachia pipientis, and (b) explores the possibility of using the current knowledge for implementing the innovative biological control methods of sterile insect technique and incompatible insect technique.
A.A. Augustinos; C.A. Moraiti; E. Drosopoulou; I. Kounatidis; P. Mavragani-Tsipidou; K. Bourtzis; N.T. Papadopoulos. Old residents and new arrivals of Rhagoletis species in Europe. Bulletin of Entomological Research 2019, 109, 701 -712.
AMA StyleA.A. Augustinos, C.A. Moraiti, E. Drosopoulou, I. Kounatidis, P. Mavragani-Tsipidou, K. Bourtzis, N.T. Papadopoulos. Old residents and new arrivals of Rhagoletis species in Europe. Bulletin of Entomological Research. 2019; 109 (6):701-712.
Chicago/Turabian StyleA.A. Augustinos; C.A. Moraiti; E. Drosopoulou; I. Kounatidis; P. Mavragani-Tsipidou; K. Bourtzis; N.T. Papadopoulos. 2019. "Old residents and new arrivals of Rhagoletis species in Europe." Bulletin of Entomological Research 109, no. 6: 701-712.
Members of the true fruit flies (family Tephritidae) are among the most serious agricultural pests worldwide, whose control and management demands large and costly international efforts. The need for cost-effective and environmentally-friendly integrated pest management (IPM) has led to the development and implementation of autocidal control strategies. Autocidal approaches include the widely used sterile insect technique (SIT) and the incompatible insect technique (IIT). IIT relies on maternally transmitted bacteria (namely Wolbachia), to cause a conditional sterility in crosses between released mass-reared Wolbachia-infected males and wild females, which are either uninfected or infected with a different Wolbachia strain (i.e., cytoplasmic incompatibility; CI). Herein, we review the current state of knowledge on Wolbachia-tephritid interactions including infection prevalence in wild populations, phenotypic consequences, and their impact on life history traits. Numerous pest tephritid species are reported to harbor Wolbachia infections, with a subset exhibiting high prevalence. The phenotypic effects of Wolbachia have been assessed in very few tephritid species, due in part to the difficulty of manipulating Wolbachia infection (removal or transinfection). Based on recent methodological advances (high-throughput DNA sequencing) and a breakthrough concerning the mechanistic basis of CI, we suggest research avenues that could accelerate generation of necessary knowledge for the potential use of Wolbachia-based IIT in area-wide integrated pest management (AW-IPM) strategies for the population control of tephritid pests.
Mariana Mateos; Humberto Martinez; Silvia B. Lanzavecchia; Claudia Conte; Karina Guillen; Brenda M. Moran-Aceves; Jorge Toledo; Pablo Liedo; Elias D. Asimakis; Vangelis Doudoumis; Georgios A. Kyritsis; Nikos T. Papadopoulos; Antonios A. Avgoustinos; Diego Fernando Segura; George Tsiamis; Kostas Bourtzis. Wolbachia pipientis associated to tephritid fruit fly pests: from basic research to applications. 2018, 358333 .
AMA StyleMariana Mateos, Humberto Martinez, Silvia B. Lanzavecchia, Claudia Conte, Karina Guillen, Brenda M. Moran-Aceves, Jorge Toledo, Pablo Liedo, Elias D. Asimakis, Vangelis Doudoumis, Georgios A. Kyritsis, Nikos T. Papadopoulos, Antonios A. Avgoustinos, Diego Fernando Segura, George Tsiamis, Kostas Bourtzis. Wolbachia pipientis associated to tephritid fruit fly pests: from basic research to applications. . 2018; ():358333.
Chicago/Turabian StyleMariana Mateos; Humberto Martinez; Silvia B. Lanzavecchia; Claudia Conte; Karina Guillen; Brenda M. Moran-Aceves; Jorge Toledo; Pablo Liedo; Elias D. Asimakis; Vangelis Doudoumis; Georgios A. Kyritsis; Nikos T. Papadopoulos; Antonios A. Avgoustinos; Diego Fernando Segura; George Tsiamis; Kostas Bourtzis. 2018. "Wolbachia pipientis associated to tephritid fruit fly pests: from basic research to applications." , no. : 358333.
The following notice was posted on the California Department of Food and Agriculture’s (CDFA) website a few weeks before we received the manuscript by Todd Shelly and his colleagues (Shelly et al. 2018) in which they attempt to rebut our claim that a number of tephritid fruit fly species, including the oriental fruit fly, Bactrocera dorsalis (Hendel), are established in the state:
James R Carey; Nikos T Papadopoulos; Richard Plant. Tephritid Pest Populations Oriental Fruit Fly Outbreaks in California: 48 Consecutive Years, 235 Cities, 1,500 Detections—and Counting. American Entomologist 2017, 63, 232 -236.
AMA StyleJames R Carey, Nikos T Papadopoulos, Richard Plant. Tephritid Pest Populations Oriental Fruit Fly Outbreaks in California: 48 Consecutive Years, 235 Cities, 1,500 Detections—and Counting. American Entomologist. 2017; 63 (4):232-236.
Chicago/Turabian StyleJames R Carey; Nikos T Papadopoulos; Richard Plant. 2017. "Tephritid Pest Populations Oriental Fruit Fly Outbreaks in California: 48 Consecutive Years, 235 Cities, 1,500 Detections—and Counting." American Entomologist 63, no. 4: 232-236.