This study aimed to evaluate the effectiveness of omega-3 supplementation with exercise in a collagenase-induced Achilles tendinopathy (AT) rat model. Experimental groups (healthy control (HC), AT, exercise (Ex), omega-3 (W), and Ex+W) were randomly allocated. After a week of adaptation, oral omega-3 was initiated for 8 weeks (5 days/week). The exercise groups performed treadmill running for 30 min/day (5 days/week, 20 m/min, 8 weeks) following one week of adaptation (10 m/min, 15 min/day). Matrix metalloproteinase-13 (MMP-13), interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α), and total antioxidant–oxidant status (TAS) levels were determined in serum samples. Tendon samples were obtained for biomechanical, histopathological, and immunohistochemical assessments. Ultimate tensile force, yield force, stiffness values, collagen type-I alpha 1 expression, and serum TAS significantly decreased (P < 0.05) in AT vs. HC. These values and expression significantly increased in the Ex+W group vs. AT. Serum MMP-13, IL-1β, and TNF-α levels decreased in all treatment groups vs. AT. The most significant decrease was found in the Ex+W group (P < 0.01). Histopathologically, the improvement in degeneration was statistically significant in the Ex+W group (P < 0.05). Immunohistochemically, MMP-13, IL-1β, TNF-α, and nitric oxide synthase-2 expression was decreased in all treatment groups vs. AT. In conclusion, omega-3 and exercise might be recommended in AT patients.

Heracleum persicum (H. percium) is a plant known for its anti-inflammatory, analgesic, anticonvulsant, and antioxidant properties. In this study, the antiproliferative effects of the ethanolic leaf and root extracts of the plant on the human Glioblastoma Multiforme U-87MG cell line were evaluated. The leaf and root extracts were applied at concentrations of 10⁻¹, 10⁻², 10⁻³, 10⁻⁴, 10⁻⁵, and 10⁻⁶ mg/mL for 24 and 72 hours, and cell viability was determined using the MTT assay. The results showed that at a concentration of 10⁻¹ mg/mL for 24 hours, the leaf and root extracts caused cell death at rates of 76% and 81%, respectively. In the 72-hour application, the leaf extract at a concentration of 10⁻¹ mg/mL led to 83% cell death, while the root extract at the same concentration caused 92% cell death. These findings demonstrate that H. persicum extracts exhibit antiproliferative effects on the U-87MG cell line and suggest their potential as therapeutic agents.

In this study, a new class of antiproliferative agents was designed and synthesized against glioblastoma, the most lethal and aggressive primary brain tumor. The structure was designed by hybridizing benzoxazole and pyrrolotriazinone, two important pharmacophore groups for antiproliferation. The target compound was synthesized in a six-step procedure. The structure of the benzoxazole-conjugated pyrrolotriazinone compound was characterized by FT-IR, 1H- and 13C-NMR spectra. It was found to have favorable drug similarity and theoretical pharmacokinetic parameters according to in silico methods. The antiproliferative effect of the target compound on the glioblastoma cell line U-87 MG was evaluated, showing dose-dependent effectiveness. Furthermore, this study investigates the molecular docking of the novel compound with key glioblastoma-associated targets, revealing its potential as a multi-target therapeutic agent. The compound demonstrates strong binding affinity and inhibitory potential against VEGFR-2, VEGFR-1, and PI3K, and shows significant activity in apoptotic pathways through Caspase-3 and BCL-2. These results suggest its potential to disrupt tumor growth, angiogenesis, and cell proliferation. Overall, the benzoxazole-conjugated pyrrolotriazinone hybrid structure shows promise for glioblastoma treatment and could represent a significant step forward in the design of new drug candidates in the future.

Peripheral nerve damage is a significant clinical problem that can lead to severe complications in patients. Regarding the regeneration of peripheral nerves, it is crucial to use experimental animals' nerves and use different evaluation methods. Epineural or perineural suturing is the gold standard in treating sciatic nerve injury, but nerve repair is often unsuccessful. This study aimed to investigate the neuroregenerative effects of magnetotherapy and bioresonance in experimental animals with sciatic nerve damage. In this study, 24 female Wistar rats were divided into 7 groups (n=6) as follows: Group 1 (Control), Group 2 (Axonotmesis control), Group 3 (Anastomosis control), Group 4 (Axonotmesis + magnetotherapy), Group 5 (Anastomosis + magnetotherapy), Group 6 (Axonotmesis + bioresonance), Group 7 (Anastomosis + bioresonance). Magnetotherapy and bioresonance treatments were applied for 12 weeks. Behavioural tests and EMG tests were performed at the end of the 12th week. Then the rats were sacrificed, and a histopathological evaluation was made. The statistical significance level was taken as 5 % in the calculations, and the SPSS (IBM SPSS for Windows, ver.21) statistical package program was used for the calculations. Statistically significant results were obtained in animal behaviour tests, EMG, and pathology groups treated with magnetotherapy. There was no statistically significant difference in the groups treated with bioresonance treatment compared to the control groups. Muscle activity and nerve repair occurred in experimental animals with acute peripheral nerve damage due to 12 weeks of magnetotherapy, and further studies should support these results.