The objective of the study was to present information about the chemical composition, the fatty acids profile, and cholesterol content of chicken meat in order to investigate the impact of chicken meat consumption on cardiovascular risk in the general population. A total of 48 6-wk-old broiler chickens broilers from two farms in June to November of 2012, and February of 2013, were used in this trial. Total lipid content was determined by extraction of fat by petrol ether (Soxhlet) after acid hydrolysis of samples. Fatty acids were determined by capillary gas chromatography. Cholesterol determination was performed by using HPLC/PDA system. The results indicate that the total free cholesterol content in raw breast and drumstick of chickens was in the range of 37,41–79,9 mg/100 g and 48,35-99,5 mg/100 g, respectively. The main fatty acids identified in all cuts were C18:1c9, C18:2n6, C16:0, C18:0, and C16:1. Decreasing the dietary n-6/n-3 clearly decreased the content in breast and drumstick muscle of C18:2n6, C18:3n3, and C20: 3n6, but increased that of C16:0, C18:0, and C20:2. Also, the major saturated fatty acid (SFA) (C16:0 and C18:0) was significantly differ among the four treatments. Our study shows that dietary fat and fatty acid composition influence the concentrations of total cholesterol content, total fat content, and fatty acid composition in broiler muscle. This information will aid in determining the burden of chicken meat as a cardiovascular risk factors disease and act as a planning tool for public-health Programmes.
The fatty acid profile, its relation to botanical origin and nutritional characteristics of bee-collected pollen from Serbia was determined. Twenty different fatty acids were detected. Depending on the palynological origin, the fatty acid (FAs) composition varied greatly. Three groups of pollen samples can be differentiated on the basis of Categorical principal component analysis. The first group was characterized by the presence of Fraxinus sp. pollen as predominant type and Zea mays as an accompanying pollen type and a high level of polyunsaturated FAs. Rannunculaceae, Apiaceae and Asteraceae family pollen, Ambrosia, Plantago, Sophora and Helianthus annuus pollen influenced the presence of saturated FAs and separation of the second group of samples. Pollen of plants belonging to the Fabaceae and Brassicaceae families had significant influence on constitution of the third group of samples with saturated and unsaturated FAs. The most abundant saturated, mono- and poly unsaturated fatty acids were palmitic, oleic and linolenic acid, respectively.