A subset of Vibrio spp. isolated from fresh Canadian mollusks (2014 to 2018) were selected for sequencing based on antimicrobial resistance profiles. The resulting de novo draft genomes include 38 Vibrio alginolyticus , 32 V. diabolicus , 10 V. parahaemolyticus , 1 V. cholerae , 1 V. ordalii , and 1 Vibrio sp. isolate.

Here, we report the high-quality complete genome sequences and plasmid arrays of Priestia megaterium ATCC 14581 ^T and of two clinical strains (2008724129 and 2008724142) isolated from human samples in the United States.

Vibrio spp. were isolated from raw shrimps imported into Canada (2009 to 2019). A total of 92 isolates with various multidrug resistance profiles were sequenced, including 59 V. parahaemolyticus , 12 V. diabolicus , 10 V. cholerae , 7 V. alginolyticus , 1 V. campbellii , 1 V. harveyi , 1 V. owensii , and 1 V. vulnificus isolate.

The intensive workload associated with the preparation of high-quality DNA libraries remains a key obstacle toward widespread deployment of sequencing technologies in remote and resource-limited areas. We describe the development of single-use microfluidic devices driven by an advanced pneumatic centrifugal microfluidic platform, the PowerBlade, to automate the preparation of Illumina-compatible libraries based on adaptor ligation methodology. The developed on-chip workflow includes enzymatic DNA fragmentation coupled to end-repair, adaptor ligation, first DNA cleanup, PCR amplification, and second DNA cleanup. This complex workflow was successfully integrated into simple thermoplastic microfluidic devices that are amenable to mass production with injection molding. The system was validated by preparing, on chip, libraries from a mixture of genomic DNA extracted from three common foodborne pathogens (Listeria monocytogenes, Escherichia coli and Salmonella enterica serovar Typhimurium) and comparing them with libraries made via a manual procedure. The two types of libraries were found to exhibit similar quality control metrics (including genome coverage, assembly, and relative abundances) and led to nearly uniform coverage independent of GC content. This microfluidic technology offers a time-saving and cost-effective alternative to manual procedures and robotic-based automation, making it suitable for deployment in remote environments where technical expertise and resources might be scarce. Specifically, it facilitates field practices that involve mid- to low-throughput sequencing, such as tasks related to foodborne pathogen detection, characterization, and microbial profiling.

Vibrio spp. isolated from fresh retail mollusk samples were selected for sequencing based on their antimicrobial resistance burden. The de novo genomes include those for Vibrio alginolyticus ( n = 48), V. diabolicus ( n = 15), V. parahaemolyticus ( n = 3), V. cholerae ( n = 2), V. metoecus ( n = 1), V. vulnificus ( n = 1), V. fluvialis ( n = 1), and unidentified Vibrio spp. ( n = 4).

We present the genome sequence of Staphylococcus aureus strain PS/BAC/317/16/W, which was isolated from contaminated platelet concentrates by the National Health Service Blood and Transplant in England (2017). Genome sequence analysis revealed the presence of one chromosome (2,665,983 bp) and two plasmids (4,265 bp and 2,921 bp) in this strain.