In order to discover new antifungal agents, twenty novel benodanil-heterocyclic carboxamide hybrids were designed, synthesized, and characterized by ¹H NMR and HRMS. In vitro, their antifungal activities against four phytopathogenic fungi were evaluated, as well as some of the target compounds at 50 mg/L demonstrated significant antifungal activities against Rhizoctonia solani. Especially, compounds 17 (EC₅₀ = 6.32 mg/L) and 18 (EC₅₀ = 6.06 mg/L) exhibited good antifungal activities against R. solani and were superior to the lead fungicide benodanil (a succinate dehydrogenase inhibitor, SDHI) (EC₅₀ = 6.38 mg/L). Furthermore, scanning electron microscopy images showed that the mycelia on treated media with the addition of compound 17 grew abnormally as compared with the negative control with tenuous, wizened, and overlapping colonies, and compounds 17 (IC₅₀ = 52.58 mg/L) and 18 (IC₅₀ = 56.86 mg/L) showed better inhibition abilities against succinate dehydrogenase (SDH) than benodanil (IC₅₀ = 62.02 mg/L). Molecular docking revealed that compound 17 fit in the gap composed of subunit B, C, and D of SDH. Furthermore, it was shown that the main interaction, one hydrogen bond interaction, was observed between compound 17 and the residue C/Trp-73. These studies suggested that compound 17 could act as a potential fungicide to be used for further optimization.

Infestations of small terrestrial mammals (e.g., rodents and lagomorphs) on the Qinghai–Tibet Plateau pose formidable challenges, which are exacerbated by the region’s vast expanse and extreme environmental conditions. Conventional chemical treatments have proven unsustainable and environmentally detrimental, highlighting the urgent need for innovative solutions. We introduce the Grassland Guidance Trap System (GGTS), an innovative control method specifically designed for ecologically sensitive plateau regions experiencing severe small terrestrial mammal infestations. In this study, we systematically investigated the capturing mechanisms of the GGTS and its sustained control effect during large–scale enclosed field deployment. The results demonstrate that the guide net achieves a 2.62–fold increase in the number of plateau pikas (Ochotona curzoniae) captured through targeted behavioral guidance; field–based behavioral trials confirm that the guide net significantly elevates plateau pikas’ observation area entries and observation area duration in adjacent areas. The open–field test demonstrated that the movement distance and activity time of plateau pikas in the corner area of the device were both significantly higher than those in the central area. In addition, rigorous 600–day field tests across 400 hm² confirmed the system’s exceptional efficacy, reducing plateau pika density by 46.38% within 100 m and 20.45% at 500 m. Notably, its effectiveness improved over time, highlighting its potential as a scalable, sustainable management solution. This research not only establishes the GGTS as a viable solution for plateau pika control but also represents a significant advancement in ecological plateau pika management, fostering long–term ecological balance on the Qinghai–Tibet Plateau.