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Mice and rats are rodent specimens commonly used in multidisciplinary research. Specifically, vasculature imaging of rodents has been widely performed in preclinical studies using various techniques, such as computed tomography, magnetic resonance imaging, and ultrasound imaging. Photoacoustic CT (PACT) is a noninvasive, nonionizing optical imaging technique derived from photoacoustic tomography and benefits from using intrinsic endogenous contrast agents to produce three-dimensional volumetric data from images. In this study, a commercial PACT device was employed to assess the cervicothoracic vasculature of mouse and rat specimens, which has rarely been examined using PACT, under two conditions with depilation and skin incision. Various blood vessels, including the common carotid artery, internal/external jugular veins, cranial vena cava, internal thoracic vein, and mammary, were identified in the acquired PACT images. The difference between the depilated and skin-incised specimens also revealed the presence of branches from certain blood vessels and specific anatomical features such as the manubrium of the sternum. This study presents detailed PACT images observing the cervicothoracic vasculature of rodent specimens and is expected to be used as a reference for various preclinical experiments on mice and rats.
Junsoo Lee; Hoseong Cho; Sungjo Park; Jaeyul Lee; Daewoon Seong; Ruchire Wijesinghe; Sangyeob Han; Shinheon Kim; Mansik Jeon; Jeehyun Kim. In Vivo Rodent Cervicothoracic Vasculature Imaging Using Photoacoustic Computed Tomography. Photonics 2021, 8, 312 .
AMA StyleJunsoo Lee, Hoseong Cho, Sungjo Park, Jaeyul Lee, Daewoon Seong, Ruchire Wijesinghe, Sangyeob Han, Shinheon Kim, Mansik Jeon, Jeehyun Kim. In Vivo Rodent Cervicothoracic Vasculature Imaging Using Photoacoustic Computed Tomography. Photonics. 2021; 8 (8):312.
Chicago/Turabian StyleJunsoo Lee; Hoseong Cho; Sungjo Park; Jaeyul Lee; Daewoon Seong; Ruchire Wijesinghe; Sangyeob Han; Shinheon Kim; Mansik Jeon; Jeehyun Kim. 2021. "In Vivo Rodent Cervicothoracic Vasculature Imaging Using Photoacoustic Computed Tomography." Photonics 8, no. 8: 312.
The main goal of this study is to develop an ultra-compact optical coherence tomography (OCT) system operated by a single-board computer to enhance its on-field applicability with superior user-friendliness and minimized system complexity. The conceptual breakthrough of this demonstration is the development of an OCT using a simple Raspberry Pi single-board computer architecture (RB-OCT) that works in a similar manner as the conventional OCT systems. The unique feature of the developed all-in-one RB-OCT system is the versatile ultra-compactness, comprising all key components of an OCT system. To further enhance the miniaturization, the micro-electro-mechanical system (MEMS) scanning unit, the entire optical configuration, and signal generating amplifier circuits were custom made to be compatible with Raspberry Pi single-board computer architecture. The dimensions of the developed system are 228 mm width, 168 mm length, and 107 mm depth with an approximate weight of 2 kg, including an image display. The software interface was developed using C++ based on Debian OS and configured using parallel programming with multi-threading. In vivo skin, ex vivo mouse cochlea, and fresh onion peels were used to evaluate the performance of the proposed system. Based on the feasibility study, this new type of single-board architecture OCT has potential merits over conventional OCT systems to be applied as a primary imaging device in various fields.
Hoseong Cho; Pilun Kim; Ruchire Eranga Wijesinghe; Hyeree Kim; Naresh Kumar Ravichandran; Mansik Jeon; Jeehyun Kim. Development of raspberry Pi single-board computer architecture based ultra-compact optical coherence tomography. Optics and Lasers in Engineering 2021, 148, 106754 .
AMA StyleHoseong Cho, Pilun Kim, Ruchire Eranga Wijesinghe, Hyeree Kim, Naresh Kumar Ravichandran, Mansik Jeon, Jeehyun Kim. Development of raspberry Pi single-board computer architecture based ultra-compact optical coherence tomography. Optics and Lasers in Engineering. 2021; 148 ():106754.
Chicago/Turabian StyleHoseong Cho; Pilun Kim; Ruchire Eranga Wijesinghe; Hyeree Kim; Naresh Kumar Ravichandran; Mansik Jeon; Jeehyun Kim. 2021. "Development of raspberry Pi single-board computer architecture based ultra-compact optical coherence tomography." Optics and Lasers in Engineering 148, no. : 106754.
Photoacoustic imaging (PAI) is a hybrid non-invasive imaging technique used to merge high optical contrast and high acoustic resolution in deep tissue. PAI has been extensively developed by utilizing its advantages that include deep imaging depth, high resolution, and label-free imaging. As a representative implementation of PAI, photoacoustic microscopy (PAM) has been used in preclinical and clinical studies for its micron-scale spatial resolution capability with high optical absorption contrast. Several handheld and portable PAM systems have been developed that improve its applicability to several fields, making it versatile. In this study, we developed a laboratory-customized, two-axis, waterproof, galvanometer scanner-based handheld PAM (WP-GVS-HH-PAM), which provides an extended field of view (14.5 × 9 mm2) for wide-range imaging. The fully waterproof handheld probe enables free movement for imaging regardless of sample shape, and volume rate and scanning region are adjustable per experimental conditions. Results of WP-GVS-HH-PAM-based phantom and in vivo imaging of mouse tissues (ear, iris, and brain) confirm the feasibility and applicability of our system as an imaging modality for various biomedical applications.
Daewoon Seong; Sangyeob Han; Jaeyul Lee; EuiMin Lee; Yoonseok Kim; Junsoo Lee; Mansik Jeon; Jeehyun Kim. Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In Vivo. Photonics 2021, 8, 305 .
AMA StyleDaewoon Seong, Sangyeob Han, Jaeyul Lee, EuiMin Lee, Yoonseok Kim, Junsoo Lee, Mansik Jeon, Jeehyun Kim. Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In Vivo. Photonics. 2021; 8 (8):305.
Chicago/Turabian StyleDaewoon Seong; Sangyeob Han; Jaeyul Lee; EuiMin Lee; Yoonseok Kim; Junsoo Lee; Mansik Jeon; Jeehyun Kim. 2021. "Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In Vivo." Photonics 8, no. 8: 305.
The aim of this study was to quantitatively assess the residual adhesive on orthodontic ceramic bracket-removed dental surface. In orthodontic process, ceramic bracket was repeated debonding physically, then the adhesive remained on the dental surface. The residual adhesive caused a lack of adhesive strength between dental and ceramic bracket. Since commonly used adhesive in orthodontics is translucent, residual adhesive is hard to be detected with conventional microscopes. Therefore, 1310 nm center wavelength swept-source OCT system based on laboratory customized image processing algorithm was used for the precise detection of residual adhesive on tooth surface. The algorithm separates residual adhesive from dental surface by comparing the height of adjacent B-scan images, while providing color-scaled images emphasizing the thickness information of residual adhesive. Finally, the acquired results were compared with microscopic and adhesive remnant index scoring gold standards, while the comparison confirmed the potential merits and the improvements of the proposed method over gold standards.
Yoonseok Kim; Gu-In Jung; Deokmin Jeon; Ruchire Wijesinghe; Daewoon Seong; Jaeyul Lee; Woo Do; Sung-Min Kwon; Jong Lee; Jun Hwang; Hyun Kim; Kyu-Bok Lee; Mansik Jeon; Jeehyun Kim. Non-Invasive Optical Coherence Tomography Data-Based Quantitative Algorithm for the Assessment of Residual Adhesive on Bracket-Removed Dental Surface. Sensors 2021, 21, 4670 .
AMA StyleYoonseok Kim, Gu-In Jung, Deokmin Jeon, Ruchire Wijesinghe, Daewoon Seong, Jaeyul Lee, Woo Do, Sung-Min Kwon, Jong Lee, Jun Hwang, Hyun Kim, Kyu-Bok Lee, Mansik Jeon, Jeehyun Kim. Non-Invasive Optical Coherence Tomography Data-Based Quantitative Algorithm for the Assessment of Residual Adhesive on Bracket-Removed Dental Surface. Sensors. 2021; 21 (14):4670.
Chicago/Turabian StyleYoonseok Kim; Gu-In Jung; Deokmin Jeon; Ruchire Wijesinghe; Daewoon Seong; Jaeyul Lee; Woo Do; Sung-Min Kwon; Jong Lee; Jun Hwang; Hyun Kim; Kyu-Bok Lee; Mansik Jeon; Jeehyun Kim. 2021. "Non-Invasive Optical Coherence Tomography Data-Based Quantitative Algorithm for the Assessment of Residual Adhesive on Bracket-Removed Dental Surface." Sensors 21, no. 14: 4670.
In otology, visualization and vibratory analysis have been crucial to enhance the success of diagnosis and surgical operation. Optical coherence tomography (OCT) has been employed in otology to obtain morphological structure of tissues non-invasively, owing to the ability of measuring the entire region of tympanic membrane, which compensates the limitations of conventional methods. As a functional extension of OCT, Doppler OCT, which enables the measurement of the motion information with structural data of tissue, has been applied in otology. Over the years, Doppler OCT systems have been evolved in various forms to enhance the measuring sensitivity of phase difference. In this review, we provide representative algorithms of Doppler OCT and various applications in otology from preclinical analysis to clinical experiments and discuss future developments.
Daewoon Seong; Changho Lee; Mansik Jeon; Jeehyun Kim. Doppler Optical Coherence Tomography for Otology Applications: From Phantom Simulation to In Vivo Experiment. Applied Sciences 2021, 11, 5711 .
AMA StyleDaewoon Seong, Changho Lee, Mansik Jeon, Jeehyun Kim. Doppler Optical Coherence Tomography for Otology Applications: From Phantom Simulation to In Vivo Experiment. Applied Sciences. 2021; 11 (12):5711.
Chicago/Turabian StyleDaewoon Seong; Changho Lee; Mansik Jeon; Jeehyun Kim. 2021. "Doppler Optical Coherence Tomography for Otology Applications: From Phantom Simulation to In Vivo Experiment." Applied Sciences 11, no. 12: 5711.
The primary optimization of the imaging speed of optical coherence tomography (OCT) has been keenly studied. In order to overcome the major speed limitation of spectral-domain OCT (SD-OCT), we developed an ultrahigh-speed SD-OCT system, with an A-scan rate of up to 1 MHz, using the method of space–time-division multiplexing (STDM). Multicameras comprising a single spectrometer were implemented in the developed ultrahigh-speed STDM method to eliminate the dead time of operation, whereas STDM was simultaneously employed to enable wide-range scanning measurements at a high speed. By successfully integrating the developed STDM method with GPU parallel processing, 8 vol/s for an image range of $250\times 250\times2048$ pixels ( $9\times 4.5\times 5$ mm) was achieved, with an adjustable volume rate according to the required scanning speed and range. The examined STDM-OCT results of the customized optical thin film confirmed its feasibility for various fields that require rapid and wide-field scanning.
Daewoon Seong; Deokmin Jeon; Ruchire Eranga Wijesinghe; Kibeom Park; Hyeree Kim; EuiMin Lee; Mansik Jeon; Jeehyun Kim. Ultrahigh-Speed Spectral-Domain Optical Coherence Tomography up to 1-MHz A-Scan Rate Using Space–Time-Division Multiplexing. IEEE Transactions on Instrumentation and Measurement 2021, 70, 1 -8.
AMA StyleDaewoon Seong, Deokmin Jeon, Ruchire Eranga Wijesinghe, Kibeom Park, Hyeree Kim, EuiMin Lee, Mansik Jeon, Jeehyun Kim. Ultrahigh-Speed Spectral-Domain Optical Coherence Tomography up to 1-MHz A-Scan Rate Using Space–Time-Division Multiplexing. IEEE Transactions on Instrumentation and Measurement. 2021; 70 ():1-8.
Chicago/Turabian StyleDaewoon Seong; Deokmin Jeon; Ruchire Eranga Wijesinghe; Kibeom Park; Hyeree Kim; EuiMin Lee; Mansik Jeon; Jeehyun Kim. 2021. "Ultrahigh-Speed Spectral-Domain Optical Coherence Tomography up to 1-MHz A-Scan Rate Using Space–Time-Division Multiplexing." IEEE Transactions on Instrumentation and Measurement 70, no. : 1-8.
Whole-directional scanning methodology is required to observe distinctive features of an entire physical structure with a three dimensional (3D) visualization. However, the implementation of whole-directional scanning is challenging for conventional optical coherence tomography (OCT), which scans a limited portion of the sample by utilizing unidirectional and bidirectional scanning methods. Therefore, in this paper an integrated quad-scanner (QS) strategy-based OCT method was implemented to obtain the whole-directional volumetry of a sample by employing four scanning arms installed around the sample. The simultaneous and sequential image acquisition capabilities are the conceptual key points of the proposed QS-OCT method, and were implemented using four precisely aligned scanning arms and applied in a complementary way according to the experimental criteria. To assess the feasibility of obtaining whole-directional morphological structures, a roll of Scotch tape, an ex vivo mouse heart, and kidney specimens were imaged and independently obtained tissue images at different directions were delicately merged to compose the 3D volume data set. The results revealed the potential merits of QS-OCT-based whole-directional imaging, which can be a favorable inspection method for various discoveries that require the dynamic coordinates of the whole physical structure.
Sm Abu Saleah; Daewoon Seong; Sangyeob Han; Ruchire Eranga Wijesinghe; Naresh Kumar Ravichandran; Mansik Jeon; Jeehyun Kim. Integrated Quad-Scanner Strategy-Based Optical Coherence Tomography for the Whole-Directional Volumetric Imaging of a Sample. Sensors 2021, 21, 1305 .
AMA StyleSm Abu Saleah, Daewoon Seong, Sangyeob Han, Ruchire Eranga Wijesinghe, Naresh Kumar Ravichandran, Mansik Jeon, Jeehyun Kim. Integrated Quad-Scanner Strategy-Based Optical Coherence Tomography for the Whole-Directional Volumetric Imaging of a Sample. Sensors. 2021; 21 (4):1305.
Chicago/Turabian StyleSm Abu Saleah; Daewoon Seong; Sangyeob Han; Ruchire Eranga Wijesinghe; Naresh Kumar Ravichandran; Mansik Jeon; Jeehyun Kim. 2021. "Integrated Quad-Scanner Strategy-Based Optical Coherence Tomography for the Whole-Directional Volumetric Imaging of a Sample." Sensors 21, no. 4: 1305.
Ixodidae tick, also known as a hard tick, is one of the major vectors of various tick-borne diseases. Studying its anatomy is the fundamental approach for diverse acarological studies and the key to understanding tick morphology. However, the conventional methods of observing internal organs rely primarily on dissection, which damages specimens irrecoverably. In this study, we developed an optical coherence tomography (OCT) system to non-invasively investigate the morphological characteristics of the hard tick. Herein, OCT imaging was conducted by the developed spectral-domain OCT (SD-OCT) system with two different objective lenses. The developed system provides an axial resolution (in the air) of 6.2 µm and a maximum lateral resolution of 2.46 µm as an objective lens with a high numerical aperture (NA) and 10× magnification was employed. Using the developed SD-OCT system, internal organs of tick specimens, such as salivary glands, midgut, genital orifice, and ovary, were identified without inflicting damage. The study suggests the feasibility of the optical coherence imaging for the acarological study of the fundamental morphological inspection and for possible future studies, such as verifying the potential morphological differences among virus transmitted hard tick specimens.
Junsoo Lee; Jihun Ryu; Sangyeob Han; Naresh Kumar Ravichandran; Daewoon Seong; Jaeyul Lee; Ruchire Eranga Wijesinghe; Pilun Kim; Seung-Yeol Lee; Hee-Young Jung; Mansik Jeon; Kwang Shik Choi; Jeehyun Kim. Identification of organs inside hard tick body using spectral-domain optical coherence tomography. Infrared Physics & Technology 2021, 114, 103611 .
AMA StyleJunsoo Lee, Jihun Ryu, Sangyeob Han, Naresh Kumar Ravichandran, Daewoon Seong, Jaeyul Lee, Ruchire Eranga Wijesinghe, Pilun Kim, Seung-Yeol Lee, Hee-Young Jung, Mansik Jeon, Kwang Shik Choi, Jeehyun Kim. Identification of organs inside hard tick body using spectral-domain optical coherence tomography. Infrared Physics & Technology. 2021; 114 ():103611.
Chicago/Turabian StyleJunsoo Lee; Jihun Ryu; Sangyeob Han; Naresh Kumar Ravichandran; Daewoon Seong; Jaeyul Lee; Ruchire Eranga Wijesinghe; Pilun Kim; Seung-Yeol Lee; Hee-Young Jung; Mansik Jeon; Kwang Shik Choi; Jeehyun Kim. 2021. "Identification of organs inside hard tick body using spectral-domain optical coherence tomography." Infrared Physics & Technology 114, no. : 103611.
Nail beautification is a widely applied gender independent practice. Excessive nail beautifications and nail-arts have a direct impact on the nail structure and can cause nail disorders. Therefore, the assessment of post-progressive nail-art effects on the nail is essential to maintain optimal nail health and to avoid any undesirable disorders. In this study, in vivo nails were examined in control stage, with a nail-art stage, and after removing the nail-art stage using a 1310 nm spectral-domain optical coherence tomography (SD-OCT) system. The acquired cross-sectional OCT images were analyzed by a laboratory customized signal processing algorithm to obtain scattered intensity profiling assessments that could reveal the effects of nail beautification on the nail plate. The formation and progression of cracks on the nail plate surface were detected as an effect of nail beautification after 72 h of nail-art removal. Changes in backscattered light intensity and nail plate thickness of control and art-removed nails were quantitatively compared. The results revealed the potential feasibility of the developed OCT-based inspection procedure to diagnose post-progressive nail-art effects on in vivo nail plate, which can be helpful to prevent nail plate damages during art removal through real-time monitoring of the boundary between the nail plate and nail-art. Besides nail-art effects, the developed method can also be used for the investigation of nail plate abnormalities by examining the inconsistency of internal and external nail plate structure, which can be diagnosed with both qualitative and quantitative assessments from a clinical perspective.
Sm Abu Saleah; Pilun Kim; Daewoon Seong; Ruchire Eranga Wijesinghe; Mansik Jeon; Jeehyun Kim. A preliminary study of post-progressive nail-art effects on in vivo nail plate using optical coherence tomography-based intensity profiling assessment. Scientific Reports 2021, 11, 1 -13.
AMA StyleSm Abu Saleah, Pilun Kim, Daewoon Seong, Ruchire Eranga Wijesinghe, Mansik Jeon, Jeehyun Kim. A preliminary study of post-progressive nail-art effects on in vivo nail plate using optical coherence tomography-based intensity profiling assessment. Scientific Reports. 2021; 11 (1):1-13.
Chicago/Turabian StyleSm Abu Saleah; Pilun Kim; Daewoon Seong; Ruchire Eranga Wijesinghe; Mansik Jeon; Jeehyun Kim. 2021. "A preliminary study of post-progressive nail-art effects on in vivo nail plate using optical coherence tomography-based intensity profiling assessment." Scientific Reports 11, no. 1: 1-13.
A bacterium in bacterial colony is a basal component of bacterial studies and is therefore of considerable importance. The morphological characteristics of a single colony have been widely used as the standard to identify diverse bacterial species. However, the conventional methods for obtaining morphological information, such as microscopic techniques, cannot provide tomographic views. This study utilizes spectral-domain optical coherence tomography (SD-OCT) to observe both external and internal structures for single colonies. OCT imaging provided a clear top (projection) and lateral (cross-sectional) images, which can identify distinct features in the fifteen different bacterial species examined. Through the acquired OCT images, the elevation type in each genus was confirmed, and the quantitative measurements for morphology including a diameter, height, and elevation angle were achieved. Moreover, the OCT raw data was used to build three-dimensional images to observe outer appearances in all direction. As a result, the averaged angles of 7.92°, 14.40°, 6.78°, and 11.92° of the genus Hymenobacter, Spirosoma, Bacillus, and Deinococcus, respectively, were calculated. In conclusion, we have demonstrated that OCT is a highly feasible, non-invasive method for the identification of bacterial species, providing detailed morphological and visual information. It thus has strong potential for applications in bacteriology, as well as biomedical and food sciences.
Junsoo Lee; Seung-Yeol Lee; Sangyeob Han; Daewoon Seong; Ruchire Eranga Wijesinghe; Pilun Kim; Mansik Jeon; Hee-Young Jung; Jeehyun Kim. Multi-directional Morphological Assessment of Single Bacterial Colonies Through Non-invasive Optical Imaging. Annals of Biomedical Engineering 2020, 48, 3014 -3023.
AMA StyleJunsoo Lee, Seung-Yeol Lee, Sangyeob Han, Daewoon Seong, Ruchire Eranga Wijesinghe, Pilun Kim, Mansik Jeon, Hee-Young Jung, Jeehyun Kim. Multi-directional Morphological Assessment of Single Bacterial Colonies Through Non-invasive Optical Imaging. Annals of Biomedical Engineering. 2020; 48 (12):3014-3023.
Chicago/Turabian StyleJunsoo Lee; Seung-Yeol Lee; Sangyeob Han; Daewoon Seong; Ruchire Eranga Wijesinghe; Pilun Kim; Mansik Jeon; Hee-Young Jung; Jeehyun Kim. 2020. "Multi-directional Morphological Assessment of Single Bacterial Colonies Through Non-invasive Optical Imaging." Annals of Biomedical Engineering 48, no. 12: 3014-3023.
The successful surgery of chronic otitis media (COM) is challenging; this depends on the surgeon's knowledge of the optical visibility of surgical microscopes. Herein, we reported the utilization of intra-surgical optical coherence tomography (OCT) system to effectively guide the surgery of COM based on augmented reality with cross-sectional images. The intra-surgical spectral-domain OCT system with a center wavelength of 846 nm was capable of obtaining non-invasive, high-resolution, and high-speed visualizations with an axial resolution of 8 μm, lateral resolution of 30 μm, and an extended working distance of 280 mm. Three patients with COM were involved in this research. The lesion conditions of the temporal bone were observed with computed tomography pre-operatively. Furthermore, pure-tone audiogram examinations were performed to evaluate pre and post-surgical conditions. The results revealed that the averaged air-bone gap of 500 Hz, 1 kHz, 2 kHz, and 4 kHz in all cases improved to 61%. Thus, the research proves that the experimental procedure can be beneficial and clinically applicable with the developed intra-surgical OCT system for future otolaryngological assessments.
Hayoung Kim; Jaeyul Lee; Ruchire Eranga Wijesinghe; Jeong Hun Jang; Mansik Jeon; Jeehyun Kim. Intra-Operative Optical Coherence Imaging of In-Vivo Chronic Otitis Media Followed by Post-Operative Audiogram Assessments. IEEE Journal of Selected Topics in Quantum Electronics 2020, 27, 1 -7.
AMA StyleHayoung Kim, Jaeyul Lee, Ruchire Eranga Wijesinghe, Jeong Hun Jang, Mansik Jeon, Jeehyun Kim. Intra-Operative Optical Coherence Imaging of In-Vivo Chronic Otitis Media Followed by Post-Operative Audiogram Assessments. IEEE Journal of Selected Topics in Quantum Electronics. 2020; 27 (4):1-7.
Chicago/Turabian StyleHayoung Kim; Jaeyul Lee; Ruchire Eranga Wijesinghe; Jeong Hun Jang; Mansik Jeon; Jeehyun Kim. 2020. "Intra-Operative Optical Coherence Imaging of In-Vivo Chronic Otitis Media Followed by Post-Operative Audiogram Assessments." IEEE Journal of Selected Topics in Quantum Electronics 27, no. 4: 1-7.
The accurate screening of otitis media (OM) lies in clarifying the numerous confounding and quantitative factors that are discovered during primary inspections. Increased awareness about bacterial biofilms and inflammation has allowed researchers to develop a better understanding of the bacterial infections that occur in the middle ear. In this study, four live guinea pigs were inoculated with Streptococcus pneumonia to induce OM-related inflammatory changes. Since optical techniques have been effectively used for diagnosis in medicine, low-coherence interferometry-based optical coherence tomography (OCT) was employed for depth-resolved high-resolution data screening. Multiple locations of the tympanic membrane (TM), mastoid mucosa, and round window membrane were examined to assess inflammatory changes. We performed qualitative assessments and thickness quantifications and investigated the variations due to adverse inflammatory affects. The findings of the present study provide a better understanding of S. pneumonia bacteria caused inflammatory changes in middle ear and it further provides a fundamental platform for future clinical utility.
Ruchire Eranga Wijesinghe; Jaeyul Lee; Deokmin Jeon; Pil Un Kim; Sangyeob Han; Junsoo Lee; Daewoon Seong; Dong-Eun Lee; Jeong Hun Jang; Mansik Jeon; Jeehyun Kim. Non-Invasive Optical Screening of Streptococcus Pneumonia Based Inflammatory Changes of the Tympanic Membrane and Mastoid Mucosa in Guinea Pig Otitis Media Using Optical Coherence Tomography. IEEE Photonics Journal 2020, 12, 1 -11.
AMA StyleRuchire Eranga Wijesinghe, Jaeyul Lee, Deokmin Jeon, Pil Un Kim, Sangyeob Han, Junsoo Lee, Daewoon Seong, Dong-Eun Lee, Jeong Hun Jang, Mansik Jeon, Jeehyun Kim. Non-Invasive Optical Screening of Streptococcus Pneumonia Based Inflammatory Changes of the Tympanic Membrane and Mastoid Mucosa in Guinea Pig Otitis Media Using Optical Coherence Tomography. IEEE Photonics Journal. 2020; 12 (4):1-11.
Chicago/Turabian StyleRuchire Eranga Wijesinghe; Jaeyul Lee; Deokmin Jeon; Pil Un Kim; Sangyeob Han; Junsoo Lee; Daewoon Seong; Dong-Eun Lee; Jeong Hun Jang; Mansik Jeon; Jeehyun Kim. 2020. "Non-Invasive Optical Screening of Streptococcus Pneumonia Based Inflammatory Changes of the Tympanic Membrane and Mastoid Mucosa in Guinea Pig Otitis Media Using Optical Coherence Tomography." IEEE Photonics Journal 12, no. 4: 1-11.
Depth-visualizing sensitivity can be degraded due to imperfect optical alignment and non-equidistant distribution of optical signals in the pixel array, which requires a measurement of the re-sampling process. To enhance this depth-visualizing sensitivity, reference and sample arm-channeled spectra corresponding to different depths using mirrors were obtained to calibrate the spectrum sampling prior to Fourier transformation. During the process, eight interferogram patterns corresponding to point spread function (PSF) signals at eight optical path length differences were acquired. To calibrate the spectrum, generated intensity points of the original interferogram were re-indexed towards a maximum intensity range, and these interferogram re-indexing points were employed to generate a new lookup table. The entire software-based process consists of eight consecutive steps. Experimental results revealed that the proposed method can achieve images with a high depth-visualizing sensitivity. Furthermore, the results validate the proposed method as a rapidly performable spectral calibration technique, and the real-time images acquired using our technique confirm the simplicity and applicability of the method to existing optical coherence tomography (OCT) systems. The sensitivity roll-off prior to the spectral calibration was measured as 28 dB and it was halved after the calibration process.
Sangyeob Han; Ruchire Eranga Wijesinghe; Deokmin Jeon; Youngmin Han; Jaeyul Lee; Junsoo Lee; Hosung Jo; Dong-Eun Lee; Mansik Jeon; Jeehyun Kim. Optical Interferometric Fringe Pattern-Incorporated Spectrum Calibration Technique for Enhanced Sensitivity of Spectral Domain Optical Coherence Tomography. Sensors 2020, 20, 2067 .
AMA StyleSangyeob Han, Ruchire Eranga Wijesinghe, Deokmin Jeon, Youngmin Han, Jaeyul Lee, Junsoo Lee, Hosung Jo, Dong-Eun Lee, Mansik Jeon, Jeehyun Kim. Optical Interferometric Fringe Pattern-Incorporated Spectrum Calibration Technique for Enhanced Sensitivity of Spectral Domain Optical Coherence Tomography. Sensors. 2020; 20 (7):2067.
Chicago/Turabian StyleSangyeob Han; Ruchire Eranga Wijesinghe; Deokmin Jeon; Youngmin Han; Jaeyul Lee; Junsoo Lee; Hosung Jo; Dong-Eun Lee; Mansik Jeon; Jeehyun Kim. 2020. "Optical Interferometric Fringe Pattern-Incorporated Spectrum Calibration Technique for Enhanced Sensitivity of Spectral Domain Optical Coherence Tomography." Sensors 20, no. 7: 2067.
Optical coherence tomography (OCT) has a micro-resolution with a penetration depth of about 2 mm and field of view of about 10 mm. This makes OCT well suited for analyzing the anatomical and internal structural assessment of the middle ear. To study the vibratory motion of the tympanic membrane (TM) and its internal structure, we developed a phase-resolved Doppler OCT system using Kasai’s autocorrelation algorithm. Doppler optical coherence tomography is a powerful imaging tool which can offer the micro-vibratory measurement of the tympanic membrane and obtain the micrometer-resolved cross-sectional images of the sample in real-time. To observe the relative vibratory motion of individual sections (malleus, thick regions, and the thin regions of the tympanic membrane) of the tympanic membrane in respect to auditory signals, we designed an experimental study for measuring the difference in Doppler phase shift for frequencies varying from 1 to 8 kHz which were given as external stimuli to the middle ear of a small animal model. Malleus is the very first interconnecting region between the TM and cochlea. In our proposed study, we observed that the maximum change in Doppler phase shift was seen for the 4 kHz acoustic stimulus in the malleus, the thick regions, and in the thin regions of the tympanic membrane. In particular, the vibration signals were higher in the malleus in comparison to the tympanic membrane.
Jeon; Kim; Deokmin Jeon; Joon Ki Kim; Mansik Jeon; Jeehyun Kim. Measurement of Vibrating Tympanic Membrane in an In Vivo Mouse Model Using Doppler Optical Coherence Tomography. Journal of Imaging 2019, 5, 74 .
AMA StyleJeon, Kim, Deokmin Jeon, Joon Ki Kim, Mansik Jeon, Jeehyun Kim. Measurement of Vibrating Tympanic Membrane in an In Vivo Mouse Model Using Doppler Optical Coherence Tomography. Journal of Imaging. 2019; 5 (9):74.
Chicago/Turabian StyleJeon; Kim; Deokmin Jeon; Joon Ki Kim; Mansik Jeon; Jeehyun Kim. 2019. "Measurement of Vibrating Tympanic Membrane in an In Vivo Mouse Model Using Doppler Optical Coherence Tomography." Journal of Imaging 5, no. 9: 74.
Non-invasive investigation of rice leaf specimens to characterize the morphological formation and particular structural information that is beneficial for agricultural perspective was demonstrated using a low coherence interferometric method called swept source optical coherence tomography (SS-OCT). The acquired results non-invasively revealed morphological properties of rice leaf, such as bulliform cells; aerenchyma, parenchyma, and collenchyma layer; and vascular bundle. Beside aforementioned morphologic characteristics, several leaf characteristics associated with cytological mechanisms of leaf rolling (leaf inclination) were examined for the pre-identification of inevitable necrosis and atrophy of leaf tissues by evaluating acute angle information, such as angular characteristics of the external bi-directional angles between the lower epidermis layer and lower mid-vein, and internal angle of lower mid-vein. To further assist the pre-identification, acquired cross-sections were employed to enumerate the small veins of each leaf specimen. Since mutants enlarge leaf angles due to increased cell division in the adaxial epidermis, healthy and abnormal leaf specimens were morphologically and quantitatively compared. Therefore, the results of the method can be used in agriculture, and SS-OCT shows potential as a rigorous investigation method for selecting mutant infected rice leaf specimens rapidly and non-destructively compared to destructive and time consuming gold-standard methods with a lack of precision.
Hyeree Kim; Xiaoxuan DU; Sungwook Kim; Pilun Kim; Ruchire Eranga Wijesinghe; Byoung-Ju Yun; Kyung-Min Kim; Mansik Jeon; Jeehyun Kim. Non-Invasive Morphological Characterization of Rice Leaf Bulliform and Aerenchyma Cellular Regions Using Low Coherence Interferometry. Applied Sciences 2019, 9, 2104 .
AMA StyleHyeree Kim, Xiaoxuan DU, Sungwook Kim, Pilun Kim, Ruchire Eranga Wijesinghe, Byoung-Ju Yun, Kyung-Min Kim, Mansik Jeon, Jeehyun Kim. Non-Invasive Morphological Characterization of Rice Leaf Bulliform and Aerenchyma Cellular Regions Using Low Coherence Interferometry. Applied Sciences. 2019; 9 (10):2104.
Chicago/Turabian StyleHyeree Kim; Xiaoxuan DU; Sungwook Kim; Pilun Kim; Ruchire Eranga Wijesinghe; Byoung-Ju Yun; Kyung-Min Kim; Mansik Jeon; Jeehyun Kim. 2019. "Non-Invasive Morphological Characterization of Rice Leaf Bulliform and Aerenchyma Cellular Regions Using Low Coherence Interferometry." Applied Sciences 9, no. 10: 2104.
An internal and marginal fit between prosthesis and abutment is an important factor of the durability of dental prosthesis. In this paper, we have proposed the use of swept-source optical coherence tomography (OCT) as a novel application for non-ionized and high-resolution measurements of internal and marginal discrepancies at anatomically critical four points, such as occlusal, angle, axial, and margin during prosthesis attachment. A tooth model was fabricated by a 3D printing technique, and the dental prosthesis was designed using dental CAD software. The cross-sectional images along with intensity peak profile analysis of the sample were acquired using the OCT system for measurements of fit. The demonstrated qualitative and quantitative evaluations can be well-utilized for assessment of the internal and marginal fit of dental prosthesis.
Sangbong Lee; KeunBaDa Son; Jaeseok Park; Jaeyul Lee; Seok Hyon Kang; Ruchire Eranga Wijesinghe; Pilun Kim; Jun Ho Hwang; Sungjo Park; Byoung-Ju Yun; Mansik Jeon; Kyu-Bok Lee; Jeehyun Kim. Non-Ionized, High-Resolution Measurement of Internal and Marginal Discrepancies of Dental Prosthesis Using Optical Coherence Tomography. IEEE Access 2018, 7, 6209 -6218.
AMA StyleSangbong Lee, KeunBaDa Son, Jaeseok Park, Jaeyul Lee, Seok Hyon Kang, Ruchire Eranga Wijesinghe, Pilun Kim, Jun Ho Hwang, Sungjo Park, Byoung-Ju Yun, Mansik Jeon, Kyu-Bok Lee, Jeehyun Kim. Non-Ionized, High-Resolution Measurement of Internal and Marginal Discrepancies of Dental Prosthesis Using Optical Coherence Tomography. IEEE Access. 2018; 7 ():6209-6218.
Chicago/Turabian StyleSangbong Lee; KeunBaDa Son; Jaeseok Park; Jaeyul Lee; Seok Hyon Kang; Ruchire Eranga Wijesinghe; Pilun Kim; Jun Ho Hwang; Sungjo Park; Byoung-Ju Yun; Mansik Jeon; Kyu-Bok Lee; Jeehyun Kim. 2018. "Non-Ionized, High-Resolution Measurement of Internal and Marginal Discrepancies of Dental Prosthesis Using Optical Coherence Tomography." IEEE Access 7, no. : 6209-6218.
Significant technical and optical advances are required for intraoperative optical coherence tomography (OCT) to be utilized during otological surgeries. Integrating OCT with surgical microscopy makes it possible to evaluate soft tissue in real-time and at a high resolution. Herein, we describe an augmented-reality, intraoperative OCT/microscope system with an extended working distance of 280 mm, providing more space for surgical manipulation than conventional techniques. We initially performed ex vivo experiments to evaluate system performance. In addition, we validated the system by performing preliminary clinical assessments of tympanomastoidectomy outcomes in six patients with chronic otitis media. The system evaluated residual inflammation in the region-of-interest of the mastoid bone. Most importantly, the system intraoperatively revealed the connection between the graft and the remnant tympanic membrane. The extended working distance allows otological surgeons to evaluate the status of both the mastoid bone and tympanic membrane during manipulation, affording full intraoperative imaging.
Jaeyul Lee; Ruchire Eranga Wijesinghe; Deokmin Jeon; Pilun Kim; Yun-Hoon Choung; Jeong Hun Jang; Mansik Jeon; Jeehyun Kim. Clinical Utility of Intraoperative Tympanomastoidectomy Assessment Using a Surgical Microscope Integrated with an Optical Coherence Tomography. Scientific Reports 2018, 8, 1 -8.
AMA StyleJaeyul Lee, Ruchire Eranga Wijesinghe, Deokmin Jeon, Pilun Kim, Yun-Hoon Choung, Jeong Hun Jang, Mansik Jeon, Jeehyun Kim. Clinical Utility of Intraoperative Tympanomastoidectomy Assessment Using a Surgical Microscope Integrated with an Optical Coherence Tomography. Scientific Reports. 2018; 8 (1):1-8.
Chicago/Turabian StyleJaeyul Lee; Ruchire Eranga Wijesinghe; Deokmin Jeon; Pilun Kim; Yun-Hoon Choung; Jeong Hun Jang; Mansik Jeon; Jeehyun Kim. 2018. "Clinical Utility of Intraoperative Tympanomastoidectomy Assessment Using a Surgical Microscope Integrated with an Optical Coherence Tomography." Scientific Reports 8, no. 1: 1-8.
Kanghae Kim; Pilun Kim; Jaeyul Lee; Suwon Kim; Sungjo Park; Soo Ho Choi; Junho Hwang; Jong Hoon Lee; Ho Lee; Ruchire Eranga Wijesinghe; Mansik Jeon; Jeehyun Kim. Non-Destructive Identification of Weld-Boundary and Porosity Formation During Laser Transmission Welding by Using Optical Coherence Tomography. IEEE Access 2018, 6, 76768 -76775.
AMA StyleKanghae Kim, Pilun Kim, Jaeyul Lee, Suwon Kim, Sungjo Park, Soo Ho Choi, Junho Hwang, Jong Hoon Lee, Ho Lee, Ruchire Eranga Wijesinghe, Mansik Jeon, Jeehyun Kim. Non-Destructive Identification of Weld-Boundary and Porosity Formation During Laser Transmission Welding by Using Optical Coherence Tomography. IEEE Access. 2018; 6 ():76768-76775.
Chicago/Turabian StyleKanghae Kim; Pilun Kim; Jaeyul Lee; Suwon Kim; Sungjo Park; Soo Ho Choi; Junho Hwang; Jong Hoon Lee; Ho Lee; Ruchire Eranga Wijesinghe; Mansik Jeon; Jeehyun Kim. 2018. "Non-Destructive Identification of Weld-Boundary and Porosity Formation During Laser Transmission Welding by Using Optical Coherence Tomography." IEEE Access 6, no. : 76768-76775.
In this study, the industrial inspection of optical thin film on touch screen panels was demonstrated using line field spectral domain optical coherence tomography. The conventional Fourier domain optical coherence tomography system requires a single scanner for two-dimensional cross-sectional images and two scanners for volumetric images. Our developed line field spectral domain optical coherence tomography has the advantage of needing only a single scanner for volumetric images, while two-dimensional cross-sectional images are obtained by the parallel acquisition of an illuminated line on a sample using an area camera. Further, the image acquisition speed was enhanced by implementing a high speed camera (340 frames per seconds) with improved quantum efficiency at near infrared region enabling two-fold frame rate. Cross-sectional and volumetric images were acquired to detect the internal sublayer defects in the optical thin films, which are difficult to observe using visual or machine vision-based inspection methods. The developed pseudo code for defect identification in optical thin films was well-utilized here for the defect inspection. The system characterization is demonstrated using United State Air Force (USAF) resolution target. The results indicate the possible application of the proposed system in touch screen panel inspection for the quality assurance of products at the consumer end.
Muhammad Faizan Shirazi; Ruchire Eranga Wijesinghe; Naresh Kumar Ravichandran; Pilun Kim; Mansik Jeon; Jeehyun Kim. Quality assessment of the optical thin films using line field spectral domain optical coherence tomography. Optics and Lasers in Engineering 2018, 110, 47 -53.
AMA StyleMuhammad Faizan Shirazi, Ruchire Eranga Wijesinghe, Naresh Kumar Ravichandran, Pilun Kim, Mansik Jeon, Jeehyun Kim. Quality assessment of the optical thin films using line field spectral domain optical coherence tomography. Optics and Lasers in Engineering. 2018; 110 ():47-53.
Chicago/Turabian StyleMuhammad Faizan Shirazi; Ruchire Eranga Wijesinghe; Naresh Kumar Ravichandran; Pilun Kim; Mansik Jeon; Jeehyun Kim. 2018. "Quality assessment of the optical thin films using line field spectral domain optical coherence tomography." Optics and Lasers in Engineering 110, no. : 47-53.
The objective of this study was to evaluate the effectiveness of optical Doppler tomography (ODT) using a portable handheld-type probe for investigating the blood flow in blood vessels of cell carcinoma-affected small animal models. To perform in vivo and real-time investigation, we developed a spectral domain-optical Doppler tomography (SD-ODT) system equipped with a portable handheld-type scanning probe, which uses the Kasai autocorrelation algorithm for Doppler imaging. The system capabilities were evaluated by conducting preliminary experiments on flow measurement in a microtube. The study was performed on healthy and squamous cell carcinoma-affected mouse specimens. The results demonstrate the possibilities and advantages of employing an SD-ODT system with handheld-type scanning probe for cell carcinoma investigation in small animal models.
Deokmin Jeon; Naresh Kumar Ravichandran; Unsang Jung; Mansik Jeon; Jeehyun Kim. Handheld-probe-based optical Doppler tomography for blood flow imaging. Infrared Physics & Technology 2018, 95, 183 -188.
AMA StyleDeokmin Jeon, Naresh Kumar Ravichandran, Unsang Jung, Mansik Jeon, Jeehyun Kim. Handheld-probe-based optical Doppler tomography for blood flow imaging. Infrared Physics & Technology. 2018; 95 ():183-188.
Chicago/Turabian StyleDeokmin Jeon; Naresh Kumar Ravichandran; Unsang Jung; Mansik Jeon; Jeehyun Kim. 2018. "Handheld-probe-based optical Doppler tomography for blood flow imaging." Infrared Physics & Technology 95, no. : 183-188.