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Rapid climate change has increased the incidence of various pests and diseases, and these threaten global food security. In particular, BLB (bacterial leaf blight) is caused by Xoo (Xanthomonas oryzae pv. oryzae) and its main characteristic is that the rice suddenly dries and withers. Recently, omics have been effectively used in agriculture. In particular, it is a key technology that can accurately diagnose diseases in the field. Until now, QTL (quantitative trait loci) mapping has been analyzed using only subjective phenotypic data by experts. However, in this study, diseases were accurately diagnosed using OCT (optical coherence tomography), and QTL mapping was performed using leaf thickness and leaf angles after Xoo inoculation. After Xoo inoculation of a 120 Cheongcheong/Nagdong double haploid (CNDH) population, QTL mapping was performed using the changing leaf angle, and OsWRKY34q1 was detected in RM811-RM14323 of chromosome 1. OsWRKY34q1 always had a higher expression level in the BLB-resistant population than in the susceptible population after Xoo inoculation. OsWRKY34q1 belongs to the WRKY family of genes. OsWRKY34q1 could be effectively used to develop BLB-resistant rice varieties in response to the current era of unpredictable climate change.
Xiao-Xuan Du; Jae-Ryoung Park; Hyeree Kim; Sm Abu Saleah; Byoung-Ju Yun; Mansik Jeon; Kyung-Min Kim. Quantitative Trait Locus Analysis of Microscopic Phenotypic Characteristic Data Obtained Using Optical Coherence Tomography Imaging of Rice Bacterial Leaf Blight Infection in the Field. Agronomy 2021, 11, 1630 .
AMA StyleXiao-Xuan Du, Jae-Ryoung Park, Hyeree Kim, Sm Abu Saleah, Byoung-Ju Yun, Mansik Jeon, Kyung-Min Kim. Quantitative Trait Locus Analysis of Microscopic Phenotypic Characteristic Data Obtained Using Optical Coherence Tomography Imaging of Rice Bacterial Leaf Blight Infection in the Field. Agronomy. 2021; 11 (8):1630.
Chicago/Turabian StyleXiao-Xuan Du; Jae-Ryoung Park; Hyeree Kim; Sm Abu Saleah; Byoung-Ju Yun; Mansik Jeon; Kyung-Min Kim. 2021. "Quantitative Trait Locus Analysis of Microscopic Phenotypic Characteristic Data Obtained Using Optical Coherence Tomography Imaging of Rice Bacterial Leaf Blight Infection in the Field." Agronomy 11, no. 8: 1630.
Mice and rats are rodent specimens commonly used in multidisciplinary research. Specifically, vasculature imaging of rodents has been widely performed in preclinical studies using various techniques, such as computed tomography, magnetic resonance imaging, and ultrasound imaging. Photoacoustic CT (PACT) is a noninvasive, nonionizing optical imaging technique derived from photoacoustic tomography and benefits from using intrinsic endogenous contrast agents to produce three-dimensional volumetric data from images. In this study, a commercial PACT device was employed to assess the cervicothoracic vasculature of mouse and rat specimens, which has rarely been examined using PACT, under two conditions with depilation and skin incision. Various blood vessels, including the common carotid artery, internal/external jugular veins, cranial vena cava, internal thoracic vein, and mammary, were identified in the acquired PACT images. The difference between the depilated and skin-incised specimens also revealed the presence of branches from certain blood vessels and specific anatomical features such as the manubrium of the sternum. This study presents detailed PACT images observing the cervicothoracic vasculature of rodent specimens and is expected to be used as a reference for various preclinical experiments on mice and rats.
Junsoo Lee; Hoseong Cho; Sungjo Park; Jaeyul Lee; Daewoon Seong; Ruchire Wijesinghe; Sangyeob Han; Shinheon Kim; Mansik Jeon; Jeehyun Kim. In Vivo Rodent Cervicothoracic Vasculature Imaging Using Photoacoustic Computed Tomography. Photonics 2021, 8, 312 .
AMA StyleJunsoo Lee, Hoseong Cho, Sungjo Park, Jaeyul Lee, Daewoon Seong, Ruchire Wijesinghe, Sangyeob Han, Shinheon Kim, Mansik Jeon, Jeehyun Kim. In Vivo Rodent Cervicothoracic Vasculature Imaging Using Photoacoustic Computed Tomography. Photonics. 2021; 8 (8):312.
Chicago/Turabian StyleJunsoo Lee; Hoseong Cho; Sungjo Park; Jaeyul Lee; Daewoon Seong; Ruchire Wijesinghe; Sangyeob Han; Shinheon Kim; Mansik Jeon; Jeehyun Kim. 2021. "In Vivo Rodent Cervicothoracic Vasculature Imaging Using Photoacoustic Computed Tomography." Photonics 8, no. 8: 312.
The main goal of this study is to develop an ultra-compact optical coherence tomography (OCT) system operated by a single-board computer to enhance its on-field applicability with superior user-friendliness and minimized system complexity. The conceptual breakthrough of this demonstration is the development of an OCT using a simple Raspberry Pi single-board computer architecture (RB-OCT) that works in a similar manner as the conventional OCT systems. The unique feature of the developed all-in-one RB-OCT system is the versatile ultra-compactness, comprising all key components of an OCT system. To further enhance the miniaturization, the micro-electro-mechanical system (MEMS) scanning unit, the entire optical configuration, and signal generating amplifier circuits were custom made to be compatible with Raspberry Pi single-board computer architecture. The dimensions of the developed system are 228 mm width, 168 mm length, and 107 mm depth with an approximate weight of 2 kg, including an image display. The software interface was developed using C++ based on Debian OS and configured using parallel programming with multi-threading. In vivo skin, ex vivo mouse cochlea, and fresh onion peels were used to evaluate the performance of the proposed system. Based on the feasibility study, this new type of single-board architecture OCT has potential merits over conventional OCT systems to be applied as a primary imaging device in various fields.
Hoseong Cho; Pilun Kim; Ruchire Eranga Wijesinghe; Hyeree Kim; Naresh Kumar Ravichandran; Mansik Jeon; Jeehyun Kim. Development of raspberry Pi single-board computer architecture based ultra-compact optical coherence tomography. Optics and Lasers in Engineering 2021, 148, 106754 .
AMA StyleHoseong Cho, Pilun Kim, Ruchire Eranga Wijesinghe, Hyeree Kim, Naresh Kumar Ravichandran, Mansik Jeon, Jeehyun Kim. Development of raspberry Pi single-board computer architecture based ultra-compact optical coherence tomography. Optics and Lasers in Engineering. 2021; 148 ():106754.
Chicago/Turabian StyleHoseong Cho; Pilun Kim; Ruchire Eranga Wijesinghe; Hyeree Kim; Naresh Kumar Ravichandran; Mansik Jeon; Jeehyun Kim. 2021. "Development of raspberry Pi single-board computer architecture based ultra-compact optical coherence tomography." Optics and Lasers in Engineering 148, no. : 106754.
Photoacoustic imaging (PAI) is a hybrid non-invasive imaging technique used to merge high optical contrast and high acoustic resolution in deep tissue. PAI has been extensively developed by utilizing its advantages that include deep imaging depth, high resolution, and label-free imaging. As a representative implementation of PAI, photoacoustic microscopy (PAM) has been used in preclinical and clinical studies for its micron-scale spatial resolution capability with high optical absorption contrast. Several handheld and portable PAM systems have been developed that improve its applicability to several fields, making it versatile. In this study, we developed a laboratory-customized, two-axis, waterproof, galvanometer scanner-based handheld PAM (WP-GVS-HH-PAM), which provides an extended field of view (14.5 × 9 mm2) for wide-range imaging. The fully waterproof handheld probe enables free movement for imaging regardless of sample shape, and volume rate and scanning region are adjustable per experimental conditions. Results of WP-GVS-HH-PAM-based phantom and in vivo imaging of mouse tissues (ear, iris, and brain) confirm the feasibility and applicability of our system as an imaging modality for various biomedical applications.
Daewoon Seong; Sangyeob Han; Jaeyul Lee; EuiMin Lee; Yoonseok Kim; Junsoo Lee; Mansik Jeon; Jeehyun Kim. Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In Vivo. Photonics 2021, 8, 305 .
AMA StyleDaewoon Seong, Sangyeob Han, Jaeyul Lee, EuiMin Lee, Yoonseok Kim, Junsoo Lee, Mansik Jeon, Jeehyun Kim. Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In Vivo. Photonics. 2021; 8 (8):305.
Chicago/Turabian StyleDaewoon Seong; Sangyeob Han; Jaeyul Lee; EuiMin Lee; Yoonseok Kim; Junsoo Lee; Mansik Jeon; Jeehyun Kim. 2021. "Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In Vivo." Photonics 8, no. 8: 305.
The aim of this study was to quantitatively assess the residual adhesive on orthodontic ceramic bracket-removed dental surface. In orthodontic process, ceramic bracket was repeated debonding physically, then the adhesive remained on the dental surface. The residual adhesive caused a lack of adhesive strength between dental and ceramic bracket. Since commonly used adhesive in orthodontics is translucent, residual adhesive is hard to be detected with conventional microscopes. Therefore, 1310 nm center wavelength swept-source OCT system based on laboratory customized image processing algorithm was used for the precise detection of residual adhesive on tooth surface. The algorithm separates residual adhesive from dental surface by comparing the height of adjacent B-scan images, while providing color-scaled images emphasizing the thickness information of residual adhesive. Finally, the acquired results were compared with microscopic and adhesive remnant index scoring gold standards, while the comparison confirmed the potential merits and the improvements of the proposed method over gold standards.
Yoonseok Kim; Gu-In Jung; Deokmin Jeon; Ruchire Wijesinghe; Daewoon Seong; Jaeyul Lee; Woo Do; Sung-Min Kwon; Jong Lee; Jun Hwang; Hyun Kim; Kyu-Bok Lee; Mansik Jeon; Jeehyun Kim. Non-Invasive Optical Coherence Tomography Data-Based Quantitative Algorithm for the Assessment of Residual Adhesive on Bracket-Removed Dental Surface. Sensors 2021, 21, 4670 .
AMA StyleYoonseok Kim, Gu-In Jung, Deokmin Jeon, Ruchire Wijesinghe, Daewoon Seong, Jaeyul Lee, Woo Do, Sung-Min Kwon, Jong Lee, Jun Hwang, Hyun Kim, Kyu-Bok Lee, Mansik Jeon, Jeehyun Kim. Non-Invasive Optical Coherence Tomography Data-Based Quantitative Algorithm for the Assessment of Residual Adhesive on Bracket-Removed Dental Surface. Sensors. 2021; 21 (14):4670.
Chicago/Turabian StyleYoonseok Kim; Gu-In Jung; Deokmin Jeon; Ruchire Wijesinghe; Daewoon Seong; Jaeyul Lee; Woo Do; Sung-Min Kwon; Jong Lee; Jun Hwang; Hyun Kim; Kyu-Bok Lee; Mansik Jeon; Jeehyun Kim. 2021. "Non-Invasive Optical Coherence Tomography Data-Based Quantitative Algorithm for the Assessment of Residual Adhesive on Bracket-Removed Dental Surface." Sensors 21, no. 14: 4670.
In otology, visualization and vibratory analysis have been crucial to enhance the success of diagnosis and surgical operation. Optical coherence tomography (OCT) has been employed in otology to obtain morphological structure of tissues non-invasively, owing to the ability of measuring the entire region of tympanic membrane, which compensates the limitations of conventional methods. As a functional extension of OCT, Doppler OCT, which enables the measurement of the motion information with structural data of tissue, has been applied in otology. Over the years, Doppler OCT systems have been evolved in various forms to enhance the measuring sensitivity of phase difference. In this review, we provide representative algorithms of Doppler OCT and various applications in otology from preclinical analysis to clinical experiments and discuss future developments.
Daewoon Seong; Changho Lee; Mansik Jeon; Jeehyun Kim. Doppler Optical Coherence Tomography for Otology Applications: From Phantom Simulation to In Vivo Experiment. Applied Sciences 2021, 11, 5711 .
AMA StyleDaewoon Seong, Changho Lee, Mansik Jeon, Jeehyun Kim. Doppler Optical Coherence Tomography for Otology Applications: From Phantom Simulation to In Vivo Experiment. Applied Sciences. 2021; 11 (12):5711.
Chicago/Turabian StyleDaewoon Seong; Changho Lee; Mansik Jeon; Jeehyun Kim. 2021. "Doppler Optical Coherence Tomography for Otology Applications: From Phantom Simulation to In Vivo Experiment." Applied Sciences 11, no. 12: 5711.
The primary optimization of the imaging speed of optical coherence tomography (OCT) has been keenly studied. In order to overcome the major speed limitation of spectral-domain OCT (SD-OCT), we developed an ultrahigh-speed SD-OCT system, with an A-scan rate of up to 1 MHz, using the method of space–time-division multiplexing (STDM). Multicameras comprising a single spectrometer were implemented in the developed ultrahigh-speed STDM method to eliminate the dead time of operation, whereas STDM was simultaneously employed to enable wide-range scanning measurements at a high speed. By successfully integrating the developed STDM method with GPU parallel processing, 8 vol/s for an image range of $250\times 250\times2048$ pixels ( $9\times 4.5\times 5$ mm) was achieved, with an adjustable volume rate according to the required scanning speed and range. The examined STDM-OCT results of the customized optical thin film confirmed its feasibility for various fields that require rapid and wide-field scanning.
Daewoon Seong; Deokmin Jeon; Ruchire Eranga Wijesinghe; Kibeom Park; Hyeree Kim; EuiMin Lee; Mansik Jeon; Jeehyun Kim. Ultrahigh-Speed Spectral-Domain Optical Coherence Tomography up to 1-MHz A-Scan Rate Using Space–Time-Division Multiplexing. IEEE Transactions on Instrumentation and Measurement 2021, 70, 1 -8.
AMA StyleDaewoon Seong, Deokmin Jeon, Ruchire Eranga Wijesinghe, Kibeom Park, Hyeree Kim, EuiMin Lee, Mansik Jeon, Jeehyun Kim. Ultrahigh-Speed Spectral-Domain Optical Coherence Tomography up to 1-MHz A-Scan Rate Using Space–Time-Division Multiplexing. IEEE Transactions on Instrumentation and Measurement. 2021; 70 ():1-8.
Chicago/Turabian StyleDaewoon Seong; Deokmin Jeon; Ruchire Eranga Wijesinghe; Kibeom Park; Hyeree Kim; EuiMin Lee; Mansik Jeon; Jeehyun Kim. 2021. "Ultrahigh-Speed Spectral-Domain Optical Coherence Tomography up to 1-MHz A-Scan Rate Using Space–Time-Division Multiplexing." IEEE Transactions on Instrumentation and Measurement 70, no. : 1-8.
Whole-directional scanning methodology is required to observe distinctive features of an entire physical structure with a three dimensional (3D) visualization. However, the implementation of whole-directional scanning is challenging for conventional optical coherence tomography (OCT), which scans a limited portion of the sample by utilizing unidirectional and bidirectional scanning methods. Therefore, in this paper an integrated quad-scanner (QS) strategy-based OCT method was implemented to obtain the whole-directional volumetry of a sample by employing four scanning arms installed around the sample. The simultaneous and sequential image acquisition capabilities are the conceptual key points of the proposed QS-OCT method, and were implemented using four precisely aligned scanning arms and applied in a complementary way according to the experimental criteria. To assess the feasibility of obtaining whole-directional morphological structures, a roll of Scotch tape, an ex vivo mouse heart, and kidney specimens were imaged and independently obtained tissue images at different directions were delicately merged to compose the 3D volume data set. The results revealed the potential merits of QS-OCT-based whole-directional imaging, which can be a favorable inspection method for various discoveries that require the dynamic coordinates of the whole physical structure.
Sm Abu Saleah; Daewoon Seong; Sangyeob Han; Ruchire Eranga Wijesinghe; Naresh Kumar Ravichandran; Mansik Jeon; Jeehyun Kim. Integrated Quad-Scanner Strategy-Based Optical Coherence Tomography for the Whole-Directional Volumetric Imaging of a Sample. Sensors 2021, 21, 1305 .
AMA StyleSm Abu Saleah, Daewoon Seong, Sangyeob Han, Ruchire Eranga Wijesinghe, Naresh Kumar Ravichandran, Mansik Jeon, Jeehyun Kim. Integrated Quad-Scanner Strategy-Based Optical Coherence Tomography for the Whole-Directional Volumetric Imaging of a Sample. Sensors. 2021; 21 (4):1305.
Chicago/Turabian StyleSm Abu Saleah; Daewoon Seong; Sangyeob Han; Ruchire Eranga Wijesinghe; Naresh Kumar Ravichandran; Mansik Jeon; Jeehyun Kim. 2021. "Integrated Quad-Scanner Strategy-Based Optical Coherence Tomography for the Whole-Directional Volumetric Imaging of a Sample." Sensors 21, no. 4: 1305.
Nail beautification is a widely applied gender independent practice. Excessive nail beautifications and nail-arts have a direct impact on the nail structure and can cause nail disorders. Therefore, the assessment of post-progressive nail-art effects on the nail is essential to maintain optimal nail health and to avoid any undesirable disorders. In this study, in vivo nails were examined in control stage, with a nail-art stage, and after removing the nail-art stage using a 1310 nm spectral-domain optical coherence tomography (SD-OCT) system. The acquired cross-sectional OCT images were analyzed by a laboratory customized signal processing algorithm to obtain scattered intensity profiling assessments that could reveal the effects of nail beautification on the nail plate. The formation and progression of cracks on the nail plate surface were detected as an effect of nail beautification after 72 h of nail-art removal. Changes in backscattered light intensity and nail plate thickness of control and art-removed nails were quantitatively compared. The results revealed the potential feasibility of the developed OCT-based inspection procedure to diagnose post-progressive nail-art effects on in vivo nail plate, which can be helpful to prevent nail plate damages during art removal through real-time monitoring of the boundary between the nail plate and nail-art. Besides nail-art effects, the developed method can also be used for the investigation of nail plate abnormalities by examining the inconsistency of internal and external nail plate structure, which can be diagnosed with both qualitative and quantitative assessments from a clinical perspective.
Sm Abu Saleah; Pilun Kim; Daewoon Seong; Ruchire Eranga Wijesinghe; Mansik Jeon; Jeehyun Kim. A preliminary study of post-progressive nail-art effects on in vivo nail plate using optical coherence tomography-based intensity profiling assessment. Scientific Reports 2021, 11, 1 -13.
AMA StyleSm Abu Saleah, Pilun Kim, Daewoon Seong, Ruchire Eranga Wijesinghe, Mansik Jeon, Jeehyun Kim. A preliminary study of post-progressive nail-art effects on in vivo nail plate using optical coherence tomography-based intensity profiling assessment. Scientific Reports. 2021; 11 (1):1-13.
Chicago/Turabian StyleSm Abu Saleah; Pilun Kim; Daewoon Seong; Ruchire Eranga Wijesinghe; Mansik Jeon; Jeehyun Kim. 2021. "A preliminary study of post-progressive nail-art effects on in vivo nail plate using optical coherence tomography-based intensity profiling assessment." Scientific Reports 11, no. 1: 1-13.
Despite the potential of a collagen construct, consisting of a major extracellular matrix component of the native cornea, as a patch graft to treat the corneal perforation, there has still been difficulty in acquiring sufficient mechanical properties for clinical availability. This study developed a novel in situ photochemical crosslinking (IPC)-assisted collagen compression process, namely, the IPC-C2 process, to significantly enhance the mechanical properties of the collagen construct for the development of a collagenous patch graft. For the first time, we found that compressed collagen construct was rapidly rehydrated in an aqueous solution, which inhibited effective riboflavin-mediated photochemical crosslinking for mechanical improvement. The IPC-C2 process was designed to concurrently induce the physical compaction and photochemical crosslinking of a compressed collagen construct, thereby avoiding the loosening of collagen fibrillar structure during rehydration and ultimately improving crosslinking efficiency. Hence, the suggested IPC-C2 process could fabricate a collagen construct with a high collagen density (∼120-280 mg ml-1) and ∼103-fold increased mechanical properties (an elastic modulus of up to ∼29 MPa and ultimate tensile strength of ∼8 MPa) compared with collagen gel. This construct can then be used as a clinically applicable collagenous patch graft. With sufficient mechanical strength for surgical suture and the controllable thickness for patient specificity, the potential of the fabricated IPC-compressed collagen construct for clinical applications was demonstrated by using an in vivo rabbit corneal perforation model. It effectively protected aqueous humor leakage and maintained the integrity of the eye globe without an additional complication.
Hyeonjun Hong; Jeongho Kim; Hoseong Cho; Sang Min Park; Mansik Jeon; Hong Kyun Kim; Dong Sung Kim. Ultra-stiff compressed collagen for corneal perforation patch graft realized by in situ photochemical crosslinking. Biofabrication 2020, 12, 045030 .
AMA StyleHyeonjun Hong, Jeongho Kim, Hoseong Cho, Sang Min Park, Mansik Jeon, Hong Kyun Kim, Dong Sung Kim. Ultra-stiff compressed collagen for corneal perforation patch graft realized by in situ photochemical crosslinking. Biofabrication. 2020; 12 (4):045030.
Chicago/Turabian StyleHyeonjun Hong; Jeongho Kim; Hoseong Cho; Sang Min Park; Mansik Jeon; Hong Kyun Kim; Dong Sung Kim. 2020. "Ultra-stiff compressed collagen for corneal perforation patch graft realized by in situ photochemical crosslinking." Biofabrication 12, no. 4: 045030.
The successful surgery of chronic otitis media (COM) is challenging; this depends on the surgeon's knowledge of the optical visibility of surgical microscopes. Herein, we reported the utilization of intra-surgical optical coherence tomography (OCT) system to effectively guide the surgery of COM based on augmented reality with cross-sectional images. The intra-surgical spectral-domain OCT system with a center wavelength of 846 nm was capable of obtaining non-invasive, high-resolution, and high-speed visualizations with an axial resolution of 8 μm, lateral resolution of 30 μm, and an extended working distance of 280 mm. Three patients with COM were involved in this research. The lesion conditions of the temporal bone were observed with computed tomography pre-operatively. Furthermore, pure-tone audiogram examinations were performed to evaluate pre and post-surgical conditions. The results revealed that the averaged air-bone gap of 500 Hz, 1 kHz, 2 kHz, and 4 kHz in all cases improved to 61%. Thus, the research proves that the experimental procedure can be beneficial and clinically applicable with the developed intra-surgical OCT system for future otolaryngological assessments.
Hayoung Kim; Jaeyul Lee; Ruchire Eranga Wijesinghe; Jeong Hun Jang; Mansik Jeon; Jeehyun Kim. Intra-Operative Optical Coherence Imaging of In-Vivo Chronic Otitis Media Followed by Post-Operative Audiogram Assessments. IEEE Journal of Selected Topics in Quantum Electronics 2020, 27, 1 -7.
AMA StyleHayoung Kim, Jaeyul Lee, Ruchire Eranga Wijesinghe, Jeong Hun Jang, Mansik Jeon, Jeehyun Kim. Intra-Operative Optical Coherence Imaging of In-Vivo Chronic Otitis Media Followed by Post-Operative Audiogram Assessments. IEEE Journal of Selected Topics in Quantum Electronics. 2020; 27 (4):1-7.
Chicago/Turabian StyleHayoung Kim; Jaeyul Lee; Ruchire Eranga Wijesinghe; Jeong Hun Jang; Mansik Jeon; Jeehyun Kim. 2020. "Intra-Operative Optical Coherence Imaging of In-Vivo Chronic Otitis Media Followed by Post-Operative Audiogram Assessments." IEEE Journal of Selected Topics in Quantum Electronics 27, no. 4: 1-7.
Digital impressions have been studied for better gingival retraction in including the under subgingival finish line condition. Here, we employed swept-source optical coherence tomography (SS-OCT) of 1310 nm wavelength, which is capable of noninvasive, high-resolution, and high-speed, to discern the utilization-possibility for supporting the fabrication of the dental crown. A three-dimensional (3D) abutment was used at the 0.5 mm of the subgingival finish line below the level of the gingiva. The SS-OCT system scanned a field of view of 10 mm × 10 mm using the 3D working model by the depth-directional three focal points. The obtained 1500 images of OCT cross-sections, which are 1221 × 1220 pixels, were rendered to the 3D model for the effective design of a virtual crown. Then, the ceramic crown was fabricated through a milling machine with a computer-aided design and computer-aided manufacturing (CAD/CAM) software. The marginal fit of the crown was evaluated 219.1 ± 48.9 μm by a silicone replica technique. Although the marginal fit is not sufficient for the clinical allowable gap at one resin typodont, this study can be anticipated to encourage further researches for the enhanced fabrication of dental crown under subgingival finish line conditions.
Sangbong Lee; KeunBaDa Son; Jaeyul Lee; Mansik Jeon; Kyu-Bok Lee; Jeehyun Kim. Fabrication of Dental Crown by Optical Coherence Tomography: A Pilot Study. IEEE Access 2020, 8, 144969 -144975.
AMA StyleSangbong Lee, KeunBaDa Son, Jaeyul Lee, Mansik Jeon, Kyu-Bok Lee, Jeehyun Kim. Fabrication of Dental Crown by Optical Coherence Tomography: A Pilot Study. IEEE Access. 2020; 8 (99):144969-144975.
Chicago/Turabian StyleSangbong Lee; KeunBaDa Son; Jaeyul Lee; Mansik Jeon; Kyu-Bok Lee; Jeehyun Kim. 2020. "Fabrication of Dental Crown by Optical Coherence Tomography: A Pilot Study." IEEE Access 8, no. 99: 144969-144975.
The accurate screening of otitis media (OM) lies in clarifying the numerous confounding and quantitative factors that are discovered during primary inspections. Increased awareness about bacterial biofilms and inflammation has allowed researchers to develop a better understanding of the bacterial infections that occur in the middle ear. In this study, four live guinea pigs were inoculated with Streptococcus pneumonia to induce OM-related inflammatory changes. Since optical techniques have been effectively used for diagnosis in medicine, low-coherence interferometry-based optical coherence tomography (OCT) was employed for depth-resolved high-resolution data screening. Multiple locations of the tympanic membrane (TM), mastoid mucosa, and round window membrane were examined to assess inflammatory changes. We performed qualitative assessments and thickness quantifications and investigated the variations due to adverse inflammatory affects. The findings of the present study provide a better understanding of S. pneumonia bacteria caused inflammatory changes in middle ear and it further provides a fundamental platform for future clinical utility.
Ruchire Eranga Wijesinghe; Jaeyul Lee; Deokmin Jeon; Pil Un Kim; Sangyeob Han; Junsoo Lee; Daewoon Seong; Dong-Eun Lee; Jeong Hun Jang; Mansik Jeon; Jeehyun Kim. Non-Invasive Optical Screening of Streptococcus Pneumonia Based Inflammatory Changes of the Tympanic Membrane and Mastoid Mucosa in Guinea Pig Otitis Media Using Optical Coherence Tomography. IEEE Photonics Journal 2020, 12, 1 -11.
AMA StyleRuchire Eranga Wijesinghe, Jaeyul Lee, Deokmin Jeon, Pil Un Kim, Sangyeob Han, Junsoo Lee, Daewoon Seong, Dong-Eun Lee, Jeong Hun Jang, Mansik Jeon, Jeehyun Kim. Non-Invasive Optical Screening of Streptococcus Pneumonia Based Inflammatory Changes of the Tympanic Membrane and Mastoid Mucosa in Guinea Pig Otitis Media Using Optical Coherence Tomography. IEEE Photonics Journal. 2020; 12 (4):1-11.
Chicago/Turabian StyleRuchire Eranga Wijesinghe; Jaeyul Lee; Deokmin Jeon; Pil Un Kim; Sangyeob Han; Junsoo Lee; Daewoon Seong; Dong-Eun Lee; Jeong Hun Jang; Mansik Jeon; Jeehyun Kim. 2020. "Non-Invasive Optical Screening of Streptococcus Pneumonia Based Inflammatory Changes of the Tympanic Membrane and Mastoid Mucosa in Guinea Pig Otitis Media Using Optical Coherence Tomography." IEEE Photonics Journal 12, no. 4: 1-11.
Dental crowns are used to restore decayed or chipped teeth, where their surfaces play a key role in this restoration process, as they affect the fitting and stable bonding of the prostheses. The surface texture of crowns can interfere with this restoration process, therefore the measurement of their inner surface roughness is very important but difficult to achieve using conventional imaging methods. In this study, the inner surfaces of dental crowns were three-dimensionally (3D) visualized using swept-source optical coherence tomography (SS-OCT) system. Nine crowns were fabricated with a commercial 3D printer using three different hatching methods (one-way, cross, and 30° angle counter-clockwise) and three different build direction angles (0°, 45°, and 90°). In addition, an image processing algorithm was developed, which uses morphological filtering, boundary detection, and a high-pass frequency filtering technique, to quantitatively evaluate the inner surface roughness of the dental crowns cross-sections with the depth-of-focus set to match two different regions. The averaged smoothness of fabricated crown was effectively produced using the cross-hatching and the build direction angle of 90° by the respective process. Thus, the results confirm the potential use of this methodology to determine the best parameters to use in 3D fabrication for improving the effectiveness and stability of dental prostheses.
Jaeyul Lee; Sm Abu Saleah; Byeonggyu Jeon; Ruchire Eranga Wijesinghe; Dong-Eun Lee; Mansik Jeon; Jeehyun Kim. Assessment of the Inner Surface Roughness of 3D Printed Dental Crowns via Optical Coherence Tomography Using a Roughness Quantification Algorithm. IEEE Access 2020, 8, 133854 -133864.
AMA StyleJaeyul Lee, Sm Abu Saleah, Byeonggyu Jeon, Ruchire Eranga Wijesinghe, Dong-Eun Lee, Mansik Jeon, Jeehyun Kim. Assessment of the Inner Surface Roughness of 3D Printed Dental Crowns via Optical Coherence Tomography Using a Roughness Quantification Algorithm. IEEE Access. 2020; 8 (99):133854-133864.
Chicago/Turabian StyleJaeyul Lee; Sm Abu Saleah; Byeonggyu Jeon; Ruchire Eranga Wijesinghe; Dong-Eun Lee; Mansik Jeon; Jeehyun Kim. 2020. "Assessment of the Inner Surface Roughness of 3D Printed Dental Crowns via Optical Coherence Tomography Using a Roughness Quantification Algorithm." IEEE Access 8, no. 99: 133854-133864.
Depth-visualizing sensitivity can be degraded due to imperfect optical alignment and non-equidistant distribution of optical signals in the pixel array, which requires a measurement of the re-sampling process. To enhance this depth-visualizing sensitivity, reference and sample arm-channeled spectra corresponding to different depths using mirrors were obtained to calibrate the spectrum sampling prior to Fourier transformation. During the process, eight interferogram patterns corresponding to point spread function (PSF) signals at eight optical path length differences were acquired. To calibrate the spectrum, generated intensity points of the original interferogram were re-indexed towards a maximum intensity range, and these interferogram re-indexing points were employed to generate a new lookup table. The entire software-based process consists of eight consecutive steps. Experimental results revealed that the proposed method can achieve images with a high depth-visualizing sensitivity. Furthermore, the results validate the proposed method as a rapidly performable spectral calibration technique, and the real-time images acquired using our technique confirm the simplicity and applicability of the method to existing optical coherence tomography (OCT) systems. The sensitivity roll-off prior to the spectral calibration was measured as 28 dB and it was halved after the calibration process.
Sangyeob Han; Ruchire Eranga Wijesinghe; Deokmin Jeon; Youngmin Han; Jaeyul Lee; Junsoo Lee; Hosung Jo; Dong-Eun Lee; Mansik Jeon; Jeehyun Kim. Optical Interferometric Fringe Pattern-Incorporated Spectrum Calibration Technique for Enhanced Sensitivity of Spectral Domain Optical Coherence Tomography. Sensors 2020, 20, 2067 .
AMA StyleSangyeob Han, Ruchire Eranga Wijesinghe, Deokmin Jeon, Youngmin Han, Jaeyul Lee, Junsoo Lee, Hosung Jo, Dong-Eun Lee, Mansik Jeon, Jeehyun Kim. Optical Interferometric Fringe Pattern-Incorporated Spectrum Calibration Technique for Enhanced Sensitivity of Spectral Domain Optical Coherence Tomography. Sensors. 2020; 20 (7):2067.
Chicago/Turabian StyleSangyeob Han; Ruchire Eranga Wijesinghe; Deokmin Jeon; Youngmin Han; Jaeyul Lee; Junsoo Lee; Hosung Jo; Dong-Eun Lee; Mansik Jeon; Jeehyun Kim. 2020. "Optical Interferometric Fringe Pattern-Incorporated Spectrum Calibration Technique for Enhanced Sensitivity of Spectral Domain Optical Coherence Tomography." Sensors 20, no. 7: 2067.
Deokmin Jeon; Unsang Jung; Kibeom Park; Pilun Kim; Sangyeob Han; Hyosang Jeong; Ruchire Eranga Wijesinghe; Naresh Kumar Ravichandran; Jaeyul Lee; Youngmin Han; Mansik Jeon; Jeehyun Kim. Vision-Inspection-Synchronized Dual Optical Coherence Tomography for High-Resolution Real-Time Multidimensional Defect Tracking in Optical Thin Film Industry. IEEE Access 2020, 8, 190700 -190709.
AMA StyleDeokmin Jeon, Unsang Jung, Kibeom Park, Pilun Kim, Sangyeob Han, Hyosang Jeong, Ruchire Eranga Wijesinghe, Naresh Kumar Ravichandran, Jaeyul Lee, Youngmin Han, Mansik Jeon, Jeehyun Kim. Vision-Inspection-Synchronized Dual Optical Coherence Tomography for High-Resolution Real-Time Multidimensional Defect Tracking in Optical Thin Film Industry. IEEE Access. 2020; 8 ():190700-190709.
Chicago/Turabian StyleDeokmin Jeon; Unsang Jung; Kibeom Park; Pilun Kim; Sangyeob Han; Hyosang Jeong; Ruchire Eranga Wijesinghe; Naresh Kumar Ravichandran; Jaeyul Lee; Youngmin Han; Mansik Jeon; Jeehyun Kim. 2020. "Vision-Inspection-Synchronized Dual Optical Coherence Tomography for High-Resolution Real-Time Multidimensional Defect Tracking in Optical Thin Film Industry." IEEE Access 8, no. : 190700-190709.
Non-invasive characterization of micro-vibrations in the tympanic membrane (TM) excited by external sound waves is considered as a promising and essential diagnosis in modern otolaryngology. To verify the possibility of measuring and discriminating the vibrating pattern of TM, here we describe a micro-vibration measurement method of latex membrane resembling the TM. The measurements are obtained with an externally generated audio stimuli of 2.0, 2.2, 2.8, 3.1 and 3.2 kHz, and their respective vibrations based tomographic, volumetric and quantitative evaluations were acquired using optical Doppler tomography (ODT). The micro oscillations and structural changes which occurred due to diverse frequencies are measured with sufficient accuracy using a highly sensitive ODT system implied phase subtraction method. The obtained results demonstrated the capability of measuring and analyzing the complex varying micro-vibration of the membrane according to implied sound frequency.
Daewoon Seong; Jaehwan Kwon; Deokmin Jeon; Ruchire Eranga Wijesinghe; Jaeyul Lee; Naresh Kumar Ravichandran; Sangyeob Han; Junsoo Lee; Pilun Kim; Mansik Jeon; Jeehyun Kim. In Situ Characterization of Micro-Vibration in Natural Latex Membrane Resembling Tympanic Membrane Functionally Using Optical Doppler Tomography. Sensors 2019, 20, 64 .
AMA StyleDaewoon Seong, Jaehwan Kwon, Deokmin Jeon, Ruchire Eranga Wijesinghe, Jaeyul Lee, Naresh Kumar Ravichandran, Sangyeob Han, Junsoo Lee, Pilun Kim, Mansik Jeon, Jeehyun Kim. In Situ Characterization of Micro-Vibration in Natural Latex Membrane Resembling Tympanic Membrane Functionally Using Optical Doppler Tomography. Sensors. 2019; 20 (1):64.
Chicago/Turabian StyleDaewoon Seong; Jaehwan Kwon; Deokmin Jeon; Ruchire Eranga Wijesinghe; Jaeyul Lee; Naresh Kumar Ravichandran; Sangyeob Han; Junsoo Lee; Pilun Kim; Mansik Jeon; Jeehyun Kim. 2019. "In Situ Characterization of Micro-Vibration in Natural Latex Membrane Resembling Tympanic Membrane Functionally Using Optical Doppler Tomography." Sensors 20, no. 1: 64.
An elevated relative moisture in the external ear canal and middle ear cavity may predispose to chronic otorrhea and related infections along with abnormal tympanic membrane (TM) vibration patterns. Therefore, phase shift‐resolved optical Doppler vibrography (ODV) was used for vibration assessments of moisture influenced cadaveric TM. ODV was applied to generate time resolved cross‐sectional and volumetric vibrographs of a cadaveric TM, driven acoustically at several frequencies. In order to analyze the effect of moisture on TM, homogenous moisture conditions were provided by soaking the cadaveric TM specimens in 1X phosphate buffer saline with a pH of 7.4. The TM specimen was exposed to a rapidly switchable frequency generator during the ODV image acquisition. The experiment was conducted for 3 hours and the cadaveric TM was exposed to each frequency with an interval of 30 minutes. Acquired phase shift‐resolved ODV assessments revealed a depth dependent vibration tendency between the applied frequencies, along with a decline in the moisture level of the cadaveric TM specimen. Thus, the ODV method can aid our understanding of sound conduction in the middle ear, thus supporting the diagnosis of TM diseases. This article is protected by copyright. All rights reserved.
Byeonggyu Jeon; Jaeyul Lee; Deokmin Jeon; Pilun Kim; Jeong Hun Jang; Ruchire Eranga Wijesinghe; Mansik Jeon; Jeehyun Kim. Functional assessment of moisture influenced cadaveric tympanic membrane using phase shift‐resolved optical Doppler vibrography. Journal of Biophotonics 2019, 13, e201900202 .
AMA StyleByeonggyu Jeon, Jaeyul Lee, Deokmin Jeon, Pilun Kim, Jeong Hun Jang, Ruchire Eranga Wijesinghe, Mansik Jeon, Jeehyun Kim. Functional assessment of moisture influenced cadaveric tympanic membrane using phase shift‐resolved optical Doppler vibrography. Journal of Biophotonics. 2019; 13 (2):e201900202.
Chicago/Turabian StyleByeonggyu Jeon; Jaeyul Lee; Deokmin Jeon; Pilun Kim; Jeong Hun Jang; Ruchire Eranga Wijesinghe; Mansik Jeon; Jeehyun Kim. 2019. "Functional assessment of moisture influenced cadaveric tympanic membrane using phase shift‐resolved optical Doppler vibrography." Journal of Biophotonics 13, no. 2: e201900202.
Optical coherence tomography (OCT) has a micro-resolution with a penetration depth of about 2 mm and field of view of about 10 mm. This makes OCT well suited for analyzing the anatomical and internal structural assessment of the middle ear. To study the vibratory motion of the tympanic membrane (TM) and its internal structure, we developed a phase-resolved Doppler OCT system using Kasai’s autocorrelation algorithm. Doppler optical coherence tomography is a powerful imaging tool which can offer the micro-vibratory measurement of the tympanic membrane and obtain the micrometer-resolved cross-sectional images of the sample in real-time. To observe the relative vibratory motion of individual sections (malleus, thick regions, and the thin regions of the tympanic membrane) of the tympanic membrane in respect to auditory signals, we designed an experimental study for measuring the difference in Doppler phase shift for frequencies varying from 1 to 8 kHz which were given as external stimuli to the middle ear of a small animal model. Malleus is the very first interconnecting region between the TM and cochlea. In our proposed study, we observed that the maximum change in Doppler phase shift was seen for the 4 kHz acoustic stimulus in the malleus, the thick regions, and in the thin regions of the tympanic membrane. In particular, the vibration signals were higher in the malleus in comparison to the tympanic membrane.
Jeon; Kim; Deokmin Jeon; Joon Ki Kim; Mansik Jeon; Jeehyun Kim. Measurement of Vibrating Tympanic Membrane in an In Vivo Mouse Model Using Doppler Optical Coherence Tomography. Journal of Imaging 2019, 5, 74 .
AMA StyleJeon, Kim, Deokmin Jeon, Joon Ki Kim, Mansik Jeon, Jeehyun Kim. Measurement of Vibrating Tympanic Membrane in an In Vivo Mouse Model Using Doppler Optical Coherence Tomography. Journal of Imaging. 2019; 5 (9):74.
Chicago/Turabian StyleJeon; Kim; Deokmin Jeon; Joon Ki Kim; Mansik Jeon; Jeehyun Kim. 2019. "Measurement of Vibrating Tympanic Membrane in an In Vivo Mouse Model Using Doppler Optical Coherence Tomography." Journal of Imaging 5, no. 9: 74.
Corneal transplantation by full‐thickness penetrating keratoplasty with human donor tissue is a widely accepted treatment for damaged or diseased corneas. Although corneal transplantation has a high success rate, a shortage of high‐quality donor tissue is a considerable limitation. Therefore, bioengineered corneas could be an effective solution for this limitation, and a decellularized extracellular matrix comprises a promising scaffold for their fabrication. In this study, three‐dimensional bioprinted decellularized collagen sheets were implanted into the stromal layer of the cornea of five rabbits. We performed in vivo noninvasive monitoring of the rabbit corneas using swept‐source optical coherence tomography (OCT) after implanting the collagen sheets. Anterior segment OCT images and averaged amplitude‐scans were acquired bi‐weekly to monitor corneal thickness after implantation for one month. The averaged cornea thickness in the control images was 430.3 ± 5.9 μm, while the averaged thickness after corneal implantation was 598.5 ± 11.8 μm and 564.5 ± 12.5 μm at two weeks and four weeks, respectively. The corneal thickness reduction of 34 μm confirmed the biocompatibility through the image analysis of the depth‐intensity profile base. Moreover, hematoxylin and eosin staining supported the biocompatibility evaluation of the bioprinted decellularized collagen sheet implantation. Hence, the developed bioprinted decellularized collagen sheets could become an alternative solution to human corneal donor tissue, and the proposed image analysis procedure could be beneficial to confirm the success of the surgery. This article is protected by copyright. All rights reserved.
Jaeseok Park; Kyoung‐Pil Lee; Hyeonji Kim; Sungjo Park; Ruchire Eranga Wijesinghe; Jaeyul Lee; Sangyeob Han; Sangbong Lee; Pilun Kim; Dong‐Woo Cho; Jinah Jang; Hong Kyun Kim; Mansik Jeon; Jeehyun Kim. Biocompatibility evaluation of bioprinted decellularized collagen sheet implanted in vivo cornea using swept‐source optical coherence tomography. Journal of Biophotonics 2019, 12, e201900098 .
AMA StyleJaeseok Park, Kyoung‐Pil Lee, Hyeonji Kim, Sungjo Park, Ruchire Eranga Wijesinghe, Jaeyul Lee, Sangyeob Han, Sangbong Lee, Pilun Kim, Dong‐Woo Cho, Jinah Jang, Hong Kyun Kim, Mansik Jeon, Jeehyun Kim. Biocompatibility evaluation of bioprinted decellularized collagen sheet implanted in vivo cornea using swept‐source optical coherence tomography. Journal of Biophotonics. 2019; 12 (11):e201900098.
Chicago/Turabian StyleJaeseok Park; Kyoung‐Pil Lee; Hyeonji Kim; Sungjo Park; Ruchire Eranga Wijesinghe; Jaeyul Lee; Sangyeob Han; Sangbong Lee; Pilun Kim; Dong‐Woo Cho; Jinah Jang; Hong Kyun Kim; Mansik Jeon; Jeehyun Kim. 2019. "Biocompatibility evaluation of bioprinted decellularized collagen sheet implanted in vivo cornea using swept‐source optical coherence tomography." Journal of Biophotonics 12, no. 11: e201900098.