The effects of organic amendments on the phytoremediation of cadmium (Cd) in acacia (Acacia mangium), jatropha (Jatropha curcas), and cassava (Manihot esculenta) were investigated. The bone meal/bat manure and leonardite/bat manure amendments resulted in better growth performance in both acacia and cassava (growth rate in dry biomass; GRDB 24.2 and 22.2, respectively), while bone meal (GRDB 17.2) was best for jatropha. The lower root/shoot ratio values of jatropha and acacia suggest that these species were better suited than cassava on Cd-contaminated soil. Cassava experienced toxicity symptoms after harvest (3 months). Acacia root accumulated somewhat greater Cd concentrations (up to 5.1 mg kg⁻¹) than cassava and jatropha roots (2.2–3.9 and 2.7–4.1 mg kg⁻¹, respectively). The bone meal and chicken manure (BMCM) treatment for jatropha had the highest bioconcentration factor for root (1.3) and the lowest translocation factor (0.7). Despite the fact that this treatment had substantial Cd concentrations in the soil (3.1 mg kg⁻¹), low Cd accumulation value (3.2 mg kg⁻¹) and the lowest Cd uptake value (127.8 mg plant⁻¹) were observed, clearly indicating that this amendment reduced Cd bioavailability. When growth performance of the study plants is considered, jatropha and acacia may be suitable for phytomanagement of Cd-contaminated soil.

It is well recognized that the aging process is a critical step in winemaking because it induces substantial chemical changes linked to the organoleptic properties and stability of the finished wines. Therefore, this study aimed to investigate the influence of different types, utilization times, and volumes of aging barrels on the metabolite profile of red wines, produced from Thai-grown Shiraz grapes, using a non-targeted proton nuclear magnetic resonance (¹H-NMR) metabolomics approach. As a result, 37 non-volatile polar metabolites including alcohols, amino acids, organic acids, carbohydrates and low-molecular-weight phenolics were identified. Chemometric analysis allowed the discrimination of wine metabolite profiles associated with different types of aging containers (oak barrels vs. stainless-steel tanks), as well as the utilization times (2, 6 and >10 years old) and volumes (225, 500 and 2000 L) of the wooden barrels employed. Significant variations in the concentration of formate, fumarate, pyruvate, succinate, citrate, gallate, acetate, tyrosine, phenylalanine, histidine, γ-aminobutyrate, methionine and choline were statistically suggested as indicators accountable for the discrimination of samples aged under different conditions. These feature biomarkers could be applied to manipulate the use of aging containers to achieve the desired wine maturation profiles.

The present study aimed to investigate the impact of Clostridium butyricum probiotic feed additive on sow and piglet performances, together with alterations in the lipidomic and metabolomic profiles of sow milk. Sixty-four Landrace × Yorkshire crossbred sows and 794 piglets were included. Sows were divided into two groups; i.e., (i) conventional gestation diet (control; n = 35) and (ii) conventional diet added with 10 g/sow/day of probiotic C. butyricum spores (treatment; n = 29) from one week before the estimated farrowing day until weaning (29.6 ± 4.8 days). The sow and piglet performances and incidence of piglet diarrhea were recorded. Changes in gross chemical composition, fatty acid and non-volatile polar metabolite profiles of sow colostrum, transient milk and mature milk were evaluated. The results showed that relative backfat loss in the treatment group (−2.3%) was significantly lower than in control group (11.6%), especially in primiparous sows (p = 0.019). The application of C. butyricum probiotics in sows significantly reduced the incidence of diarrhea in piglets (p < 0.001) but no other effect on piglet performance was found. Lipidomic and metabolomic analyses revealed variations in sow colostrum and milk biomolecular profiles, with indicative compounds significantly altered by feeding with the C. butyricum probiotics. In conclusion, the use of C. butyricum probiotics in sows may improve sow body condition and reduce diarrhea incidence in piglets, with underlying changes in milk composition that warrant further investigation. These findings support the potential of C. butyricum as a beneficial feed additive in swine production.

In vitro fecal inoculation coupled with gas chromatography–mass spectrometry (GC-MS) has been used for evaluating fecal deodorants. However, high cost and complex data interpretation limit its routine application. An electronic nose (eNose) offers a rapid, cost-effective alternative. This study aimed to evaluate the eNose as a screening tool for fecal odor compared with solid-phase microextraction gas chromatography–mass spectrometry (SPME GC-MS) and to examine the in vitro effects of fecal deodorant supplements on fecal odor profiles. Feces from ten healthy cats were serially diluted (1:1 to 1:8) and analyzed using both instruments. Four dietary supplements—Yucca schidigera extract (YSE), Quillaja saponaria extract (QSE), fructooligosaccharides (FOS), and oat beta-glucans (OBG)—were tested at concentrations of 0.0, 0.2, 0.4, and 0.8 g/100 mL. The eNose showed comparable performance to GC-MS in discriminating among sample dilutions. In vitro fermentation showed that FOS and OBG significantly increased volatile fatty acid (VFA)-related sensor responses while signals linked to ammonia and sulfur compounds were reduced. QSE had minimal effect, whereas YSE produced moderate changes. The total sensor response intensities did not differ between treatments. These findings indicate that prebiotic supplements exert stronger effects than saponin-based supplements and highlight the potential of eNoses with in vitro fermentation for rapid screening of fecal deodorants.

Environmental temperature-induced metabolic changes in dams can be reflected by alterations in metabolomic and fatty acid profiles in colostrum. The colostrum from 13 Black Bengal (BB) dams was collected on the day of parturition at two consecutive parities during the hot conditions (HCs) of summer or rainy seasons and the cold conditions (CCs) of winter. The metabolomic and fatty acid profiles were analyzed using nuclear magnetic resonance (NMR) and gas chromatography–mass spectrometry, respectively. The results showed significantly higher sarcosine, tyrosine, citrate, succinate, galactose, acetylglucosamine, carnitine, choline, glycerophosphocholine, and trimethylamine N-oxide during CCs than HCs; potential discriminant metabolites according to VIP scores were sarcosine, succinate, and choline. Colostrum from CCs had significantly lower levels of saturated fatty acids (SFAs), including butyric acid (C4:0), myristic acid (C14:0), and pentadecanoic acid (C15:0), but higher omega-9 monounsaturated fatty acids (MUFAs), especially oleic acid (C18:1n9c), elaidic acid (C18:1n9t), and eicosenoic acid (C20:1n9), than in HC. Linoleic acid (C18:2n6c) and the omega 6/omega 3 PUFA ratio were higher during CCs than HCs. It is concluded that a metabolic shift for nutrient utilization occurs, from glucose during HCs toward fat during CCs, which may not be due to the diet but rather neurohumoral alterations occurring during temperature adaptation.

Acacia (Acacia mangium), jatropha (Jatropha curcas), and cassava (Manihot esculenta) were cultivated in a greenhouse to see how organic amendments affected plant survival and accumulation of cadmium (Cd) in plant tissues. The study plants are bioenergy crops, which are advantageous to phytomanagement because they provide a significant economic benefit to local residents and farmers in the agricultural sector while also simultaneously reducing Cd entry into food webs through consumption. In this study, bone meal/bat manure and leonardite/bat manure were the key organic amendments that promoted the best growth performances in acacia (growth rate in dry biomass; GRDB 24.2) and cassava (GRDB 22.2), respectively, while jatropha preferred bone meal (GRDB 17.2). However, leonardite/bone meal considerably reduced Cd uptake values in whole plant tissues of acacia and cassava (35 mg plant^-1 and 119.1 mg plant^-1, respectively), while bone meal/chicken manure greatly reduced Cd uptake values in jatropha (127.8 mg plant^-1). Cassava is a Cd excluder; however, it may not be a useful bioenergy crop to cultivate in Cd soil because it displayed toxicity symptoms after harvesting. The best plant for phytomanagement in this study was jatropha, which demonstrated substantial growth biomass and Cd accumulation, followed by acacia.