This paper presents the design, simulation and low-cost fabrication of microfluidic channel for biomedical application. Channel is fabricated using soft lithography technique. Printed Circuit Board (PCB) is used to make the master for the channel. Channel pattern is transferred on PCB plate using toner transfer technique followed by ferric chloride etching. Paper also discusses, the issues involved in PCB based master fabrication and their viable solutions. Glass is used as substrate material and the channel is made of Sylgard 184 Polydimethylsiloxane (PDMS). Channel is interfaced with a syringe pump to observe the fluid flow. To predict the behavior of the channel, FEM simulation is performed using COMSOL Multiphysics 5.2a. There is a good match between the theoretical, simulation and test results. Finally, to test the biocompatibility of the channel, genomic DNA is passed through the channel and gel electrophoresis analysis is performed.

Circular Capacitance Micromachined Ultrasonic Transducer

Protein secondary structure prediction involves determining a protein's secondary structure from its primary amino acid sequence, serving as a critical step toward tertiary structure prediction. This, in turn, is essential for applications in drug design, protein engineering, and genetic research. Given the complexity of this task, advanced methods such as Gated Recurrent Units (GRUs) and Long Short-Term Memory (LSTM) networks are often employed, as they effectively capture long-range dependencies between amino acids, thereby improving prediction accuracy. In this study, we utilized the latter, specifically Bidirectional Long Short-Term Memory (BLSTM) networks, which process protein sequences in both forward and backward directions. This bidirectional processing has shown considerable promise in this domain. To further enhance local feature extraction, the network architecture incorporates a local feature encoding and extraction module consisting of three 1-dimensional convolutional layers, designed to capture dependencies between adjacent amino acids. Several optimization and regularization techniques were applied to refine the model, including batch normalization, kernel initialization, kernel regularization, dropout, and pooling layers. Optimal values for each parameter were identified through meticulous hyperparameter tuning. The final proposed model, termed Deep Convolutional BLSTM (DCBLSTM), was evaluated on three publicly available and widely recognized datasets: CB513, CASP10, and CASP11. For Q8-state classification, the model achieved accuracies of 88.9%, 83.9%, and 84.3%, respectively, on these datasets. These results demonstrate that the proposed model delivers state-of-the-art accuracy, outperforming several existing benchmark models. The consistently high accuracy highlights the effectiveness and robustness of the DCBLSTM model for protein secondary structure prediction.

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Quantitative estimation of Embelin from Embelia tsjeriam-Cottam A.DC. and in vitro multiplication for production of quality planting stock