Skin injury is the most common type of injury, which manifests itself in the form of wounds and cuts. A minor wound repairs itself within a short span of time. However, deep wounds require adequate care and sometime clinical interventions such as surgical suturing for their timely closure and healing. In literature, mechanical properties of skin and other tissues are well known. However, the anisotropic behavior of wounded skin has not been studied yet, specifically with respect to localized overstraining and possibilities of rupture. In the current work, the biomechanics of common skin wound geometries were studied with a biofidelic skin phantom, using uniaxial mechanical testing and Digital Image Correlation (DIC). Global and local mechanical properties were investigated, and possibilities of rupture due to localized overstraining were studied across different wound geometries and locations. Based on the experiments, a finite element (FE) model was developed for a common elliptical skin wound geometry. The fidelity of this FE model was evaluated with simulation of uniaxial tension tests. The induced strain distributions and stress-stretch responses of the FE model correlated very well with the experiments (R² > 0.95). This model would be useful for prediction of the mechanical response of common wound geometries, especially with respect to their chances of rupture due to localized overstraining. This knowledge would be indispensable for pre-surgical planning, and also in robotic surgeries, for selection of appropriate wound closure techniques, which do not overstrain the skin tissue or initiate tearing.

Electroporation is used in medicine for drug and gene delivery, and as a nonthermal ablation method in tumor treatment and cardiac ablation. Electroporation involves delivering high-voltage electric pulses to target tissue; however, this can cause effects beyond the intended target tissue like nerve stimulation, muscle contractions and pain, requiring use of sedatives or anesthetics. It was previously shown that adjusting pulse parameters may mitigate some of these effects, but not how these adjustments would affect electroporation’s efficacy. We investigated the effect of varying pulse parameters such as interphase and interpulse delay while keeping the duration and number of pulses constant on nerve stimulation, muscle contraction and assessing pain and electroporation efficacy, conducting experiments on human volunteers, tissue samples and cell lines in vitro. Our results show that using specific pulse parameters, particularly short high-frequency biphasic pulses with short interphase and long interpulse delays, reduces muscle contractions and pain sensations in healthy individuals. Higher stimulation thresholds were also observed in experiments on isolated swine phrenic nerves and human esophagus tissues. However, changes in the interphase and interpulse delays did not affect the cell permeability and survival, suggesting that modifying the pulse parameters could minimize adverse effects while preserving therapeutic goals in electroporation.

Surgical repair of hernia and prolapse with prosthetic meshes are well-known to cause pain, infection, hernia recurrence, and mesh contraction and failures. In literature, mesh failure mechanics have been studied with uniaxial, biaxial, and cyclic load testing of dry and wet meshes. Also, extensive experimental studies have been conducted on surrogates, such as non-human primates and rodents, to understand the effect of mesh stiffness, pore size, and knitting patterns on mesh biocompatibility. However, the mechanical properties of such animal tissue surrogates are widely different from human tissues. Therefore, to date, mechanics of the interaction between mesh and human tissues is poorly understood. This work addresses this gap in literature by experimentally and computationally modeling the biomechanical behavior of mesh, sutured to human tissue phantom under tension. A commercially available mesh (Prolene^®) was sutured to vaginal tissue phantom material and tested at different uniaxial strains and strain rates. Global and local stresses at the tissue phantom, suture, and mesh were analyzed. The results of this study provide important insights into the mechanics of prosthetic mesh failure and will be indispensable for better mesh design in the future.