Sargassum is a marine product, source of bioaktive compound with many divers biological activity, especially as antioxidant and anticancer. The bioactive compound of Sargassum  have been utilized as health product in many Industry such as, food, medicine and cosmetic. Fatty acids, (E)-11-octyloxacyclododec-10-en-2-one were isolated from ethyl acetic fraction of brown seaweed Sargassum crassifolium. The aim of the research is to isolate and purification the fatty acid compound and measure the pigmen content and antioxidant activity of S. crassifolium. The method of reasearc as followed; Sargassum extracted using methanol after that the extract partitioned with hexane and ethyl acetic and rinsed with methanol using Colomn Chromatography (CC). Ethyl acetic was purificated and isolated using CC and Thin Layer Chromatography (TLC) was using to monitored and observed the compounds. Isolate of pure compounds of etil acetic fraction of S. crassifolium (FEtSar) was elucidated by basic NMR spectroscopy, FTIR and ESMS and compared with previous literature. The extract, fractions and isolated compound were tested for their pigment content (carotenoid and fucoxanthin) and antioxidant activity using DPPH. The result of study shown, extract, fraction and pure compound of ethyl acetate fraction of S. crassifolium, (FEtSar), (E)-11-octyloxacyclododec-10-en-2-one revealed have antioxidant compound. Therefore S. crassifolium is potencial as a source of natural antioksidant and in future could be explored as health product.

Cervical cancer remains a major global health burden, particularly in low- and middle-income countries, necessitating the discovery of novel, accessible anticancer agents. Marine macroalgae, including Halymenia durvillaei , are rich in bioactive compounds with promising therapeutic potential. H. durvillaei collected from North Sulawesi, Indonesia, was extracted with methanol and fractionated into n-hexane, chloroform, ethyl acetate, and aqueous fractions. Phytochemical profiling and cytotoxicity were assessed using the brine shrimp lethality test (15.625–1000 µg/mL) and MTT assay against HeLa cells treated with extract fractions (0.125–2 mg/mL) for 72 h. The most bioactive fraction was subjected to GC–MS characterization. Major compounds identified were evaluated through molecular docking against Vaccinia H1-related phosphatase (VHR; PDB ID: 3F81) using AutoDock Vina/CB-Dock2, followed by 100 ns molecular dynamics simulation and ADMET prediction. Phytochemical screening confirmed the presence of flavonoids (2.91 mg QE/g), alkaloids (1.03 mg/g), tannins, saponins, and steroids. The chloroform fraction exhibited the highest cytotoxicity (LC₅₀ = 33.83 µg/mL), while ethyl acetate, n-hexane, chloroform, and aqueous fractions inhibited 70–93% of HeLa cell proliferation. GC–MS analysis identified oleic acid as a major compound. In silico studies revealed stable binding of oleic acid to VHR phosphatase, a protein implicated in cervical cancer progression, supported by favorable ADMET properties. H. durvillaei demonstrates significant cytotoxic and antiproliferative activities, highlighting its potential as a source of natural anticancer agents. However, further compound isolation and mechanistic validation are required to confirm its therapeutic relevance.

The potential of seaweed as a source of bioactive compounds including antioxidants made it a versatile form of ingredient that can be incorporated directly or indirectly in the preparation of foods and beverages. Antioxidant constituents are important in managing and regulating oxidative stress and protecting the human body from severe adverse effects due to the excess release of free radicals or reactive oxygen species that promote the onset of chronic diseases. This study aimed to make a functional beverage containing 20 – 30% Caulerpa racemosa (seaweed), and identify phytocompounds using GC–MS, determine the antioxidant activity, molecular and drug likenees of the identfied compounds. Data showed that the total phenolic content of the juice made with 30% seaweed was 32.2 + 0.5 μg/GAE, vitamin C 2.9 + 0.1 µg/mL while the antioxidant activity (DPPH IC50) was 46.8±3.3 mg/mL. Twenty compounds were identified of which three met the Lipinski’s rule of five. They were then docked with five enzymes involved in oxidative stress and inflammation (cytochrome P450, lipoxygenase, myeloperoxidase, NADPH oxidase, and Xanthin oxidase). Their binding energies (∆G: – 4.1 to – 6.3 Kcal/mol) were comparable to those of known commercial binders (∆G: – 5.0 to – 7.3 Kcal/mol) of each enzyme. Pharmacokinetic analyses showed excellent scores for the three compounds in regard to bioavailability, human intestinal absorption, and blood brain barrier. The sensory test value of the juice showed good odor, taste, and color, with a score between 4.7- 5.9. Overall, the functionalized juice displayed properies that make it potentially useful in the reduction of oxidative stress are related conditions, although in-vivo studies are needed.