Plant-associated microbiomes play important roles in plant health and productivity. However, despite fruits being directly linked to plant productivity, little is known about the microbiomes of fruits and their potential association with fruit health. Here, by integrating 16S rRNA gene, ITS high-throughput sequencing data and microbiological culturable approaches, we reported that roots and fruits (pods) of peanut, a typical plant that bears fruits underground, recruit different bacterial and fungal communities independently of cropping conditions, and that the incidence of pod disease under monocropping conditions is attributed to the depletion of Bacillus genus and enrichment of Aspergillus genus in geocarposphere. On this basis, we constructed a synthetic community (SynCom) consisting of three Bacillus strains from geocarposphere soil under rotation conditions with high culturable abundance. Comparative transcriptome, microbiome profiling and plant phytohormone signaling analysis reveal that the SynCom exhibited more effective Aspergillus growth inhibition and pod disease control than individual strain, which was underpinned by a combination of molecular mechanisms related to fungal cell proliferation interference, mycotoxins biosynthesis impairment and jasmonic acid-mediated plant immunity activation. Overall, our results reveal the filter effect of plant organs on the microbiome, and that depletion of key protective microbial community promotes the fruit disease incidence.

In 2011–2014, ELISA or nucleic acid spot hybridization (NASH) testing for common potato viruses or Potato spindle tuber viroid (PSTVd) was performed on 500 leaf samples collected in potato fields in the northeast provinces Heilongjiang and Inner Mongolia, China. The results revealed that 38.4% (Heilongjiang) and 27.7% (Inner Mongolia) were positive for Potato virus Y (PVY). To unveil the strain composition and population structure of PVY in the region, the multiplex RT-PCR described by Chikh-Ali et al. was performed on all of the ELISA-PVY-positive samples. Of the 158 samples whose PVY strain scenarios could be determined, PVY^NTN-NW-SYR-II and PVY^N-Wi were the most abundant strains, occurring in 58.9 and 47.5% samples, followed by PVY^NTN-NW-SYR-I (31.0%), PVY^N:O (19.6%), Eu-PVY^NTN (7.6%), NA-PVY^N (1.3%), and PVY^O (0.6%). In the 84 single-strain-infected samples, PVY^N-Wi accounted for 41.7%, PVY^NTN-NW-SYR-II for 40.5%, PVY^NTN-NW-SYR-I for 14.3%, and PVY^N:O and Eu-PVY^NTN for 3.6% each. Seven isolates representing PVY^NTN-NW-SYR-I (HLJ-6-1 and HLJ-9-4), PVY^NTN-NW-SYR-II (INM-W-369-12 and SC-1-1-2), PVY^N:O (HLJ-30-2), and PVY^N-Wi (HLJ-BDH-2 and HLJ-C-429) were sequenced and analyzed molecularly. Whereas the sequence identities for isolates belonging to the same strain group were >98.5%, they fell for isolates belonging to different strain groups to 92.7–98.1% at the genome level and 96.1–98.4% at the polyprotein level. Interestingly, the exact location of the recombination events varied among isolates within a strain group. Phylogenetic analysis of all 42 full length PVY sequences from China indicated that most clustered to various recombinant groups, despite the fact that the PVY isolates were isolated from at least five host species. Pathological analysis of four isolates representing PVY^N:O, PVY^N-Wi, PVY^NTN-NW-SYR-I, and PVY^NTN-NW-SYR-II revealed that the PVY^NTN-NW-SYR-II isolate incited the most severe symptoms on potato cultivar Kexin 13, followed by PVY^NTN-NW-SYR-I, PVY^N:O and PVY^N-Wi. The PVY^NTN-NW-SYR-I and PVY^NTN-NW-SYR-II isolates also caused necrotic ringspots on the tubers of Kexin 13, indicating their ability to induce the potato tuber necrotic ringspot disease in potato.

Nitrogen application at the beginning flowering stage (R1 stage) increased the soybean grain yield, however, the rapid effect of enriched nitrogen at R1 growth stage on soybean dry matter accumulation and sugar metabolism is still unclear. Continuous high nitrogen (CHN), Continuous low nitrogen (CLN), Enriched nitrogen supply at R1 stage (ENS) treatments were applied on two soybean cultivars (Liaodou11, Liaodou14), to investigate the effect of enriched nitrogen on plant biomass accumulation and sucrose metabolism. After 12 h of ENS treatment, the root/shoot rate of both cultivars were lower than that of CLN, but at 24 h it was no significant difference between ENS and CLN. Enriched N at R1 stage, soybean kept a balance of sucrose synthesis and decomposition in leaf by affecting sucrose synthetase (SS) and sucrose phosphate synthase (SPS) activities. Under N limitation condition the plant dry matter accumulation supported root growth priority. Enriched N at R1 stage resulted in the rapid shoot biomass accumulation. In high yield cultivar, the shoot growth was priority to root growth, the common yield cultivar was on the contrary. Our result suggest that enrich N at R1 stage resulted in the accumulation of biomass in shoot rapidly.

The family Botryosphaeriaceae is species rich and includes pathogens, saprobes and endophytes of economically important agricultural crops and plants. As pathogens, Botryosphaeriaceae species employ evolving defensive and counter-defensive strategies that enable them to infect their hosts. Molecular genetic results showed high genetic variability among Botryosphaeriaceae species, which could be a key determinant to their differing environmental roles on hosts and differences in their virulence. Improved technologies such as whole genome re-sequencing, integrated RNA-Seq, comparative and ab initio approaches for molecular genetics and mutation analysis have revealed possible virulence factors that might be involved in the pathogenicity of these fungi. Several genes involved in the pathogenicity processes of botryosphaeriaceous fungi have been identified and characterized. There are numerous reports on the involvement of phytotoxic metabolites in the pathogenicity of these taxa. Availability of whole genomes, genetic transformation and transcriptome analysis of some botryosphaeriaceous species have contributed towards identifying the biological functions of many genes in a fast and accurate way, enabling these fungi to be used as model organism for molecular research of plant opportunistic fungal pathogens in woody plants. The research on botryosphaeriaceous species will provide understanding of the infection mechanisms, and with designing control strategies against diseases caused by opportunistic fungal pathogens.

Endophytic symbioses between plants and fungi are a dominant feature of many terrestrial ecosystems, yet little is known about the signaling that defines these symbiotic associations. Hydrogen peroxide (H₂O₂) is recognized as a key signal mediating the plant adaptive response to both biotic and abiotic stresses. However, the role of H₂O₂ in plant-fungal symbiosis remains elusive. Using a combination of physiological analysis, plant and fungal deletion mutants, and comparative transcriptomics, we reported that various environmental conditions differentially affect the interaction between Arabidopsis and a root endophyte Phomopsis liquidambaris, and link this process to alterations in H₂O₂ levels and H₂O₂ fluxes across root tips. We found that enhanced H₂O₂ efflux leading to a moderate increase in H₂O₂ levels at the plant-fungal interface is required for maintaining plant-fungal symbiosis. Disturbance of plant H₂O₂ homeostasis compromises the symbiotic ability of plant roots. Moreover, the fungus-regulated H₂O₂ dynamics modulate the rhizosphere microbiome by selectively enriching for the phylum Cyanobacteria, with strong antioxidant defenses. Our results demonstrated that the regulation of H₂O₂ dynamics at the plant-fungal interface affects the symbiotic outcome in response to external conditions and highlight the importance of root endophyte in reshaping the rhizosphere microbiota.