The biostimulation potentials of carrot peel waste and carob kibbles for bioremediation of crude petroleum-oil polluted soil were investigated. Temperature, pH, moisture, total petroleum hydrocarbon (TPH), and changes in microbial counts during 45 days were monitored when 4 mL of carrot peel waste or carob kibbles media were added to 200 g of crude oil polluted soil samples. Gas chromatography-flame ionization detection (GC-FID) was used to compare hydrocarbon present in the crude oil polluted soil and in pure fuel, composition of crude oil polluted soil was analyzed by X-ray diffraction (XRD), and the TPH was measured by distillation using distiller mud. The results showed that, at the end of experiments, the concentration of TPH decreased in crude oil polluted soil containing carrot peel waste with a percentage of 27 ± 1.90% followed by crude oil polluted soil containing carob kibbles (34 ± 1.80%) and in the unamended control soil (36 ± 1.27%), respectively. The log [Colony Forming Unit (CFU)/g] of total heterotrophic bacteria in the crude oil polluted soil increased from 10.46 ± 0.91 to 13.26 ± 0.84 for carrot peel waste, from 11.01 ± 0.56 to 11.99 ± 0.77 for carob kibbles and from 8.18 ± 0.39 to 8.84 ± 0.84 for control, respectively. Such results demonstrated that carrot peel could be used to enhance activities of the microbial hydrocarbon-degrading bacteria during bioremediation of crude petroleum-oil polluted soil.

The present work is conducted as part of the development and the valorization of bioactive natural substances from Algerian medicinal and aromatic spontaneous plants, a clean alternative method in biological control. For this purpose, the bio-acaricidal activity of Salvia officinalis (sage)essential oil (EO)was evaluated against the Varroa destructor, a major threat to the honey bee Apis mellifera ssp. intermissa. The aerial parts of S. officinalis L., 1753 were collected from the Chrea mountainous area in Northern Algeria. They were subjected to hydro distillation by a Clevenger apparatus type to obtain the EO, and screened for bio-acaricidal activity against Varroa destructor by the method of strips impregnated with the mixture EO and twin according to three doses. Pre-treatment results revealed infestation rates in the experimental site ranging from 3.76% to 21.22%. This showed the heterogeneity of infestations in hives according to the density of bees. This constituted a difficulty in monitoring the population dynamics of this parasite. After treatment, a difference in the acaricidal effect of Sage essential oil is noticed. It gives a mortality rate of 6.09% by the dose D1: 5%, 2.32% by the dose D2: 15%, and a low mortality rate of 0.9% by the dose D3: 20%. The chemical treatment carried out by Bayvarol gives a result close to that of the essential oil of Sage (9.97%).These results point to the fact that Sage essential oil treatments have a significant effect and good biological activity with regard to harmful species.