Centella asiatica extract (CAE) and Marigold extract (ME) have been renowned for centuries in traditional medicine, particularly in Asia, for their wound‐healing, anti‐inflammatory and skin‐regenerative properties. This study presents the formulation and comprehensive evaluation of serums enhanced with these extracts, aiming to harness their bioactive properties for skin health. Serums were selected over creams due to their lightweight, water‐based nature, which ensures faster absorption and enhanced skin penetration of active ingredients. The serums, developed in varying concentrations (0.2%, 0.5% and 1.0%), were assessed for physicochemical properties, stability, bioactivity and skin barrier enhancement. Stability tests confirmed the serums' robustness, maintaining consistent pH (5.0–6.5), viscosity and organoleptic properties over 60 days. The MTT assay revealed that both extracts were non‐cytotoxic, maintaining cell viability above 100%, with formulations containing CAE, demonstrating a concentration‐dependent proliferative effect, reaching nearly 120%. Antibacterial evaluation using the agar well diffusion assay showed that both extracts exhibited considerable activity, with CAE achieving greater inhibition zones, particularly against S. aureus . Antioxidant, anti‐inflammatory and anti‐tyrosinase activities confirmed the strong bioactive potential of both extracts in combating oxidative stress, reducing inflammation, and regulating pigmentation. Skin barrier and permeability studies demonstrated the serums' ability to improve barrier integrity and facilitate active compound delivery. These findings demonstrate the potential of Centella asiatica and Marigold extracts for multifunctional skincare formulations, combining regeneration, hydration, antioxidant and antibacterial benefits.

This study presents the development of a novel multifunctional hydrogel biocomposite sponge designed to address the complexities of wound healing, including rapid hemostasis, infection prevention, and tissue regeneration. Recognizing the limitations of conventional wound dressings that lack multifunctionality, this study introduces a 3D chitosan/tannic acid (CS/TA) hydrogel. After testing three chitosan/tannic acid (CS/TA) ratios, CS/TA-1 (1:0.16), CS/TA-2 (1:0.25), and CS/TA-3 (1:0.34), the most effective formulation, CS/TA-2, was enhanced with sodium alginate (SA) and arginine (Arg) for optimal performance. Arginine, with its guanidinium functional group, served as a green crosslinker through physical interactions, enhancing the sponge's mechanical strength while also improving its hemostatic performance and biocompatibility, promoting cellular interactions. Its inclusion significantly amplified antioxidant activity (>90 %), mitigating oxidative stress and contributing to enhanced therapeutic outcomes. Ionic crosslinking and freeze-drying created a porous, absorbent sponge with high water retention and compression resilience. SEM confirmed the sponge's interconnected porosity, enabling cell infiltration and nutrient exchange. Blood Clotting Index (BCI) assessments demonstrated the hemostatic effectiveness of CS/TA/SA/Arg-3, with 25 % BCI at 5 min and 20 % at 15 min, along with excellent hemocompatibility, achieving a 2.08 % hemolysis rate. These results suggest the hydrogel sponge's potential for effective wound management in emergencies and clinical applications.

The development of sustainable packaging materials with advanced functional properties is a key priority for the food industry. In this study, chitosan (CS)-based nanocomposite films incorporating titanium dioxide (TiO₂), zinc oxide (ZnO), hybrid ZnO_TiO₂ nanoparticles, lignin (LG), and nanolignin (nLG) were synthesized and comprehensively characterized. Structural analyses (FTIR, XRD, SEM) confirmed strong intermolecular interactions and homogeneous nanoparticle dispersion, particularly for TiO₂ and low ZnO concentrations. Mechanical testing showed that TiO₂ and ZnO significantly enhanced tensile strength (up to fourfold) and elongation at break. Among the prepared nanocomposite films, CS-TiO₂ films at 2 wt% exhibited the best balance of mechanical performance and antioxidant activity. Subsequent incorporation of LG and especially nLG into the CS-TiO₂ matrix further enhanced flexibility and toughness, antioxidant efficiency, and radical-scavenging activity above 90%, and improved UV-shielding capacity by reducing light transmittance. Moreover, antibacterial testing against Escherichia coli demonstrated that CS/TiO₂/nLG films achieved the highest reduction (~46%), attributed to synergistic electrostatic, oxidative, and phenolic mechanisms. Overall, CS/TiO₂/nLG nanocomposites emerge as multifunctional, biodegradable films with significant potential for next-generation active food packaging applications.

Two hemostatic bionanocomposite dressings were developed using natural, semi-natural (or semi-synthetic) and synthetic polymers. The first system consisted of chitosan (CS), polyvinyl alcohol (PVA), and carboxymethyl cellulose (CMC) (CS/PVA/CMC), while the second was based on CS, PVA, and starch (SR) (CS/PVA/SR). Zinc oxide (ZnO) nanoparticles and bicyclic monoterpene camphor (CP) ketone were incorporated as bioactive agents in order to enhance antimicrobial and hemostatic performance. FTIR spectroscopy confirmed the successful solvent casting synthesis of the dressings and the interactions between the biopolymers and additives. XRD analysis indicated a predominantly amorphous structure, while SEM images and EDS analysis revealed uniform dispersion of ZnO particles within the polymer matrices without aggregation. Furthermore, the CS/PVA/CMC-1ZnO/CP sample exhibited a water sorption of 12,666 ± 126%, while CS/PVA/SR-1ZnO/CP reached 7013 ± 215%. ZnO incorporation also improved mechanical performance, with CS/PVA/SR-2ZnO/CP displaying the highest tensile strength (39.18 ± 0.2 MPa) and elongation at break (9.54 ± 1.04%). ZnO incorporation also led to a concentration-dependent increase in antibacterial activity, with SR-based dressings achieving near-complete bacterial reduction at higher ZnO loadings. All the dressings demonstrated good biocompatibility, while CS/PVA/SR-1ZnOCP showed the fastest clotting time (420 s ± 40), highlighting its potential for hemostatic applications.