Objectives: Cupping therapy (CT) is an ancient medical treatment since antiquity and is used for the treatment of such various disease states as contagious diseases, chronic or acute inflammatory disease, and autoimmune disorders. Ventricular repolarization is represented by QT and corrected-QT (QTc) intervals from surface ECG. As novel repolarization parameters, Tpeak-toTend (Tp-Te) interval, and Tp-Te/QT and Tp-Te/QTc ratios are suggested to correlate better with ventricular arrhythmia risk in various clinical conditions than sole QT and QTc intervals. In this study, we aimed to determine whether these parameters changed significantly after cupping therapy in healthy individuals. Methods: One hundred and twenty subjects (57 women and 63 men; mean age 49.0 ± 13.0 years) participated in this study. ECGs strips were recorded 1 hour before and 1 hour after cupping therapy from each participant, and relevant ECG parameters were compared. Results: No statistically significant change was observed in mean heart rate, QT and QTc intervals, QT/QRS and cQT/QRS, and frontal QRS/T angle after the procedure compared with the same parameters before the procedure (p >0.05). However, Tp-Te interval [69.51 ± 11.54 msec vs 63.15 ± 10.89 msec, p= 0.001], Tp-Te/QT ratio [0.191 ± 0.030 vs 0.174 ± 0.031, p=0.002] and Tp-Te/QTc ratio [0.175 ± 0.030 vs 0.159 ± 0.026, p=0.001] were found to be significantly decreased 1 hour following the procedure compared with the pre-procedure values. Conclusions: According to the results of our study, it is plausible to conclude that CT may exert cardioprotective effect. However, larger-scale prospective studies are needed to support our findings.