The goal of this study was to build an understanding of the quality of Idesia polycarpa fruit Maxim from different ecotypes and to identify the best cultivars, with a view to providing a reference and theoretical basis for the selection and cultivation of I. polycarpa. In this study, we systematically evaluated the fruit quality characteristics of five seed sources, namely SH, SG1, GG, HX, and SG2, at four developmental stages, M1-M4, through a principal component analysis, a correlation analysis, and a significance test. Comparisons between the ecotype yielded that GG was significantly better than the other ecotype in oil content (28.7%) and fresh weight per cluster (155.56 g), while HX exhibited higher SOD content (278.18 U/g) and soluble protein content (27.50 mg·g⁻¹), suggesting a higher level of stress tolerance. The results of the correlation analysis showed that POD was significantly negatively correlated with oil content (r = −0.633) and SOD (r = −0.617) activities, indicating that the antioxidant enzyme system may affect oil accumulation. The results of the principal component analysis showed that the cumulative contribution of the first four principal components reached 89.72%, of which principal component 1 mainly reflected yield-related traits, and principal component 2 was significantly correlated with oil content and soluble protein. Through the evaluation and screening of the five ecotypes, we determined that GG can be utilized as a good single plant in the selection and improvement of new cultivars; our findings can provide theoretical support for the selection of good cultivars of I. polycarpa seed in the central region of Henan.

Botryosphaeria dothidea (Moug. ex Fr.) Ces. & De Not. is a major pathogenic fungus causing stem canker in Idesia polycarpa, posing a significant threat to the growth and survival of its plantations. To elucidate the physiological and biochemical responses of the host under pathogenic stress, this study used two-year-old potted seedlings of I. polycarpa (provenance: ‘Emeishan No. 1’) and conducted artificial inoculation. Dynamic changes in physiological and biochemical indices at different disease stages were systematically monitored. The results revealed a distinct stage-specific response pattern: in the early infection stage, the activities of antioxidant enzymes (POD and SOD) increased rapidly, accompanied by significant elevations in osmotic regulators such as proline and soluble protein. In the mid-stage, malondialdehyde (MDA) content increased, while the levels of photosynthetic pigments—especially chlorophyll b and carotenoids—declined, indicating intensified oxidative damage. In the late stage, antioxidant capacity and osmotic adjustment weakened, and the photosynthetic system was continuously impaired. Correlation analysis further demonstrated significant synergistic relationships among antioxidant defense, membrane stability, osmotic regulation, and photosynthetic function. These findings enhance our understanding of the disease resistance mechanisms in I. polycarpa and provide a theoretical and practical reference for resistance evaluation and precise management of canker disease in woody species.

In the context of sustainable forest resource development, balancing ecological conservation with rational utilization is essential to achieving forest multifunctionality. Longyuwan National Forest Park, located in Luanchuan County, Henan Province, serves as a transitional zone between rural mountainous ecosystems and nearby urban settlements. Increasingly, this area faces urbanization pressures such as tourism expansion, infrastructure development, and intensified land use, which may threaten ecological stability. This study aims to evaluate the ecological sensitivity of the park and optimize its spatial functional zoning. Using the Analytic Hierarchy Process (AHP), we followed four key steps: constructing the hierarchical model, generating the pairwise judgment matrices, computing the weights and conducting the consistency check, and determining the final weights. A hierarchical evaluation framework was constructed using the AHP, incorporating twelve ecological indicators across geomorphological, hydrological, atmospheric, biological, and anthropogenic dimensions. Spatial analysis tools in ArcGIS 10.2, including reclassification and weighted overlay, were employed for single-factor and integrated sensitivity assessments. The results indicated that land-use type, elevation, and water-body distribution were the most influential indicators. Ecological sensitivity across the park was categorized into five levels: extremely high (0.02%), high (11.99%), moderate (73.53%), low (14.19%), and extremely low (0.28%). Based on these findings, four functional zones were delineated: ecological conservation (50.99%), core landscape (22.86%), general recreation (23.94%), and management and service (2.21%). This research provides spatially explicit insights into forest management under anthropogenic stress, offering theoretical support for the sustainable governance of forest–urban interface landscapes.

Understory intercropping may improve soils, but its effects on bacterial communities in Idesia polycarpa plantations remain unclear. Five patterns were established in the Yellow River floodplain: pure I. polycarpa and I. polycarpa intercropped with Astragalus membranaceus, Ipomoea batatas, Salvia rosmarinus, or Arachis hypogaea. Soil properties and bacterial communities were assessed using 16S rRNA gene sequencing, LEfSe, FAPROTAX, and redundancy analysis. Intercropping significantly affected soil electrical conductivity, water content, organic matter, alkali-hydrolyzable nitrogen, available phosphorus, and available potassium, but not pH or bulk density. Salvia rosmarinus intercropping had 14.3 g kg⁻¹ organic matter and 123 mg kg⁻¹ available potassium; Arachis hypogaea had 14.5 g kg⁻¹ organic matter and 81.3 mg kg⁻¹ alkali-hydrolyzable nitrogen; and Ipomoea batatas had 11.9% water content and 13.6 mg kg⁻¹ available phosphorus. Alpha diversity did not differ significantly. PCoA suggested differentiation, but PERMANOVA based on Bray–Curtis dissimilarity was nonsignificant (r² = 0.329, p = 0.083); LEfSe identified treatment-associated biomarkers. Chemoheterotrophy and aerobic chemoheterotrophy accounted for 42.6–45.7% and 20.4–26.2%, respectively. Alkali-hydrolyzable nitrogen was associated with genus-level variation (r² = 0.440, p = 0.031). Understory intercropping improved soil conditions and may influence bacterial composition and predicted functional potential.

Idesia polycarpa Maxim. is an important woody oilseed species and is dioecious; however, systematic evidence for sex-specific differences in leaf physico-chemical traits and their spectral responses remains limited. In this study, mature female and male trees were investigated. Leaves were sampled throughout the growing season (May–October), and FTIR-ATR spectra were acquired to derive peak height and peak area metrics for diagnostic bands. In parallel, leaf antioxidant enzyme activities (SOD, CAT, POD, and APX), biomass-related traits, leaf nutrient concentrations, and rhizosphere soil nutrient indices were measured. Differences between sexes, seasonal dynamics, and spectrum–trait coupling were evaluated using repeated-measures analysis and correlation analyses. The results showed that the positions of major absorption bands were largely consistent between sexes, indicating broadly similar chemical composition, whereas the male plants lacked an obvious band near 1671 cm⁻¹ in May. Several spectral peak parameters were significantly correlated with leaf pH, leaf dry matter content, total phosphorus, and APX activity. Female and male plants exhibited month-dependent differences in enzyme activities, dry matter content, and leaf N and K, and leaf–soil nutrient linkages were also detected, suggesting sex-specific resource allocation patterns. Overall, FTIR-ATR peak metrics provide a rapid means to characterize seasonal variation in leaf physico-chemical properties of I. polycarpa and offer supporting evidence for studies of sexual dimorphism.

Objective: This study aimed to clarify the variation law of phenotypic traits such as the stem, leaf, flower, main branch ear and seed of Prunella vulgaris Linnaeus. (P. vulgaris), providing provide basic data support for protecting its wild germplasm resources. The phenotypic traits of stems, leaves, flowers, main branch ears and seeds of different wild populations of P. vulgaris were measured to reveal the phenotypic variation among and within populations of P. vulgaris, and to provide a scientific basis for the protection, utilization and collection of wild P. vulgaris germplasm resources. Methods: Variance analysis, coefficient of variation analysis, multiple comparison analysis, cluster analysis and principal component analysis were used to study the phenotypic traits of nine wild P. vulgaris populations in different regions of China. Results: The average phenotypic differentiation coefficient of nine traits was 52.04%. Inter-population variation was the main source of phenotypic variation of P. vulgaris. The average variation coefficient of each trait in the population was 21.25%, and the variation range was 15.02–28.94%. Pearson correlation analysis showed that there was a strong autocorrelation between phenotypic traits of wild P. vulgaris, and spikes and stems were greatly affected by environmental factors. According to the diameter of the stem and ear, nine wild P. vulgaris from different geographical populations could be divided into three groups. Group III (AHHS, JXFY) had the best phenotypic traits, and the traits of stem height, stem diameter, leaf length, leaf width and main branch ear length were the largest. The contribution rate of the three principal components screened by principal component analysis was 85.78%. Conclusions: We believe that the phenotypic differentiation among wild populations of P. vulgaris is at a medium level, and the variation within the population is smaller than that between populations, which may be an important reason for the sharp decline in the wild resources of P. vulgaris.