Idesia polycarpa Maxim. is valued for its high oil yield, which fruit has high oil content and good health effects. However, the large amount of unsaturated fatty acids in the oil is easily oxidized, and its storage intolerance has seriously restricted its marketing. In order to slow down the oxidation rate of Idesia polycarpa Maxim. oil, this study found that the most effective single antioxidant was rosemary extract through the screening of seven different natural antioxidants. The experiments were designed using the response surface method to compound three individual natural antioxidants with good anti-oxidative effects, namely rosemary extract, tea polyphenol palmitate, and ascorbic acid palmitate. A comprehensive model was established to determine the optimal anti-oxidative compound formulation, which was found to be 0.45 g/kg of rosemary extract +0.21 g/kg of tea polyphenol palmitate. The effectiveness of the best compounded natural antioxidants under simulated frying temperature (160 °C) was then examined. The results showed that the antioxidant capacity of the compound formulation was significantly increased, and the generation of secondary oxidation products was inhibited and the loss of unsaturated fatty acids was reduced. Therefore, the compound antioxidant-added Idesia polycarpa Maxim. oil not only showed good storage resistance, but also improved the safety of use under high temperature environment. The results of this study significantly improved the oxidative stability of Idesia polycarpa Maxim. oil, provided a theoretical basis for replacing synthetic antioxidants, promoted the industrial development of Idesia polycarpa Maxim. oil and expanded other applications.

The goal of this study was to build an understanding of the quality of Idesia polycarpa fruit Maxim from different ecotypes and to identify the best cultivars, with a view to providing a reference and theoretical basis for the selection and cultivation of I. polycarpa. In this study, we systematically evaluated the fruit quality characteristics of five seed sources, namely SH, SG1, GG, HX, and SG2, at four developmental stages, M1-M4, through a principal component analysis, a correlation analysis, and a significance test. Comparisons between the ecotype yielded that GG was significantly better than the other ecotype in oil content (28.7%) and fresh weight per cluster (155.56 g), while HX exhibited higher SOD content (278.18 U/g) and soluble protein content (27.50 mg·g⁻¹), suggesting a higher level of stress tolerance. The results of the correlation analysis showed that POD was significantly negatively correlated with oil content (r = −0.633) and SOD (r = −0.617) activities, indicating that the antioxidant enzyme system may affect oil accumulation. The results of the principal component analysis showed that the cumulative contribution of the first four principal components reached 89.72%, of which principal component 1 mainly reflected yield-related traits, and principal component 2 was significantly correlated with oil content and soluble protein. Through the evaluation and screening of the five ecotypes, we determined that GG can be utilized as a good single plant in the selection and improvement of new cultivars; our findings can provide theoretical support for the selection of good cultivars of I. polycarpa seed in the central region of Henan.

The Luohe River boasts a profound historical heritage. Due to long-term impacts of human activities along its banks, significant variations in soil environmental conditions may exist across different land use types within the region. This study focused on four land use types (farmland, bamboo forest, grassland, and abandoned land) in Luoning County of the Luohe River Basin and employed high-throughput sequencing technology to analyze the characteristics of soil microbial communities and differences in soil nutrients. The results showed the following: There were significant differences in soil nutrients and microbial diversity among different land use types. Specifically, the organic matter content in farmland was significantly higher than that in bamboo forests (p < 0.05), and the available phosphorus content in farmland was significantly higher than that in abandoned land (p < 0.05); the abandoned land had a significant advantage in alkali-hydrolyzable nitrogen and available potassium contents (p < 0.05) but the lowest soil water content (p < 0.05). Microbial diversity indices indicated that Pielou’s evenness index (Pieloue) in farmland was significantly higher than that in grassland. The bacterial community was dominated by Acidobacteria, Proteobacteria, and Actinobacteria. At the genus level, available potassium was the key factor affecting the top 20 dominant bacterial genera. Redundancy Analysis (RDA) showed that pH was the core environmental variable driving the variation of bacterial community structure. Metabolic pathway analysis revealed that biosynthetic metabolism was the main pathway, and grassland exhibited outstanding performance in the secondary metabolite synthesis pathway. The results of this study fill the gap in soil microbial ecology research in this region and provide a theoretical basis for the sustainable utilization of land resources and agricultural ecological management in the Luohe River Basin.

Botryosphaeria dothidea (Moug. ex Fr.) Ces. & De Not. is a major pathogenic fungus causing stem canker in Idesia polycarpa, posing a significant threat to the growth and survival of its plantations. To elucidate the physiological and biochemical responses of the host under pathogenic stress, this study used two-year-old potted seedlings of I. polycarpa (provenance: ‘Emeishan No. 1’) and conducted artificial inoculation. Dynamic changes in physiological and biochemical indices at different disease stages were systematically monitored. The results revealed a distinct stage-specific response pattern: in the early infection stage, the activities of antioxidant enzymes (POD and SOD) increased rapidly, accompanied by significant elevations in osmotic regulators such as proline and soluble protein. In the mid-stage, malondialdehyde (MDA) content increased, while the levels of photosynthetic pigments—especially chlorophyll b and carotenoids—declined, indicating intensified oxidative damage. In the late stage, antioxidant capacity and osmotic adjustment weakened, and the photosynthetic system was continuously impaired. Correlation analysis further demonstrated significant synergistic relationships among antioxidant defense, membrane stability, osmotic regulation, and photosynthetic function. These findings enhance our understanding of the disease resistance mechanisms in I. polycarpa and provide a theoretical and practical reference for resistance evaluation and precise management of canker disease in woody species.

Idesia polycarpa is a multipurpose tree species valued for both fruit production and timber and is often referred to as a “beautiful oil reservoir on trees”. However, research has largely focused on fruits, whereas the metabolic composition and potential value of other tissues, such as leaves and shoot tips, remain insufficiently characterized. Here, we employed UPLC–MS/MS-based untargeted metabolomics to compare metabolite profiles and functional components between the leaves and shoot tips of I. polycarpa. In total, 378 differential metabolites were identified, with 105 significantly upregulated in leaves and 273 significantly upregulated in shoot tips. Among these, 32 metabolites were detected exclusively in shoot tips, and 22 were unique to leaves. Leaves were enriched in secondary metabolites, particularly flavonoids and phenolic acids, with scutellarin and catechol as representative compounds. In contrast, shoot tips exhibited higher levels of primary metabolites, including nucleotides and their derivatives as well as amino acids, with catechin and epicatechin being representative compounds. In addition, targeted metabolomics was performed to quantify 53 flavonoids in leaves; scutellarin (2088.1350 μg/g) and apigenin-7-O-glucuronide (1020.9085 μg/g) showed the highest concentrations. Collectively, these results reveal tissue-specific metabolic characteristics in I. polycarpa and provide a data basis for future functional studies and the comprehensive utilization of leaves and shoot tips.

Idesia polycarpa ‘Yitong 2’ is a high-oil cultivar widely promoted in central China, yet field evidence on how soil bacterial communities respond during early plantation establishment remains limited. Here, we conducted fixed-site monitoring in a newly established ‘Yitong 2’ plantation in the Luohe River Basin (Henan, China). Bulk soil (0–30 cm) was collected before planting (March 2024) and at 3, 6 and 12 months after planting (June 2024, September 2024 and March 2025). Soil physicochemical properties were measured and bacterial communities were profiled by 16S rRNA gene (V3–V4) amplicon sequencing; functional potential was inferred using PICRUSt2. Available potassium increased significantly, whereas soil organic matter showed a decrease–recovery trajectory. Bacterial richness (Chao1) decreased after planting, while evenness increased; Shannon diversity remained stable. Community composition shifted directionally, with higher relative abundance of Pseudomonadota (formerly Proteobacteria) and reduced Acidobacteriota at later stages. PERMANOVA based on Bray–Curtis distances indicated significant temporal differences in community structure. RDA indicated that soil organic matter and bulk density were the primary drivers of community structural variation. Functionally, the overall metabolic framework remained stable, whereas pathways related to genetic information processing and metabolism exhibited significant differences (p < 0.05). By examining both intra-annual dynamics and inter-annual changes in soil bacteria and physicochemical properties following the planting of ‘Yitong 2’, this study clarifies patterns of soil property variation and trajectories of microbial community structure and functional potential, thereby providing a scientific basis for the establishment of high-quality I. polycarpa plantations and the sustainable development of soil ecosystems.