The Pikad Tri-phol-sa-mut-than (TS) remedy, a Thai traditional medicine, is officially recorded in Tamra Paetsart Sonkrau Chabub Anurak for its capabilities in treating kidney deficiency. TS remedy is composed of three fruit species—Aegle marmelos (L.) Corrêa., Coriandrum sativum L., and Morinda citrifolia L.—in an equal part by weight. The quality of the raw material is one of the essential factors that can affect the effectiveness and safety of treatment by herbal remedy. The pharmacognostic evaluation and DNA barcode of the three fruit species and TS remedy were performed in this study to authenticate them from contamination, and to provide the scientific database for further uses. Macroscopic and microscopic examination, chemical profile by TLC, and DNA barcoding were employed to positively identify the raw materials bought from the herbal market, especially the powder form. Consequently, the outcomes of this investigation can be used to develop an essential and effective tool for the authentication of crude drugs and herbal remedies.

The Wannachawee Recipe (WCR) is a traditional Thai herbal formulation with a clinical history of use in psoriasis. An observational study conducted at Prapokklao Hospital reported that 93% of psoriasis patients showed good clinical responses. However, the absence of standardized quality control parameters remains a critical barrier to its pharmaceutical reproducibility, safety, and integration into mainstream clinical practice. This study established robust quality specifications and a phytochemical profiling for WCR, in accordance with the Thai Herbal Pharmacopoeia (THP) guidelines, to support its development from traditional use to a standardized therapeutic agent. A multimodal analytical approach was employed, integrating microscopic characterization, physicochemical evaluation, and advanced instrumental techniques. Phytochemical characterization was conducted using High-Performance Liquid Chromatography (HPLC) fingerprinting and Compact Mass Spectrometry (CMS). A validated HPLC method was developed to quantify trans-p-coumaryl alcohol, a key bioactive marker. Anti-inflammatory activity was further assessed by measuring inhibition of nitric oxide production. Physicochemical analysis established rigorous benchmarks, including ethanol-soluble extractive (8.73 ± 0.15% w/w), water-soluble extractive (18.89 ± 0.09% w/w), and loss on drying (<10%), which ensure long-term stability and microbial safety. CMS analysis successfully identified key chemical constituents, including alpha-amyrin, stemone, protocatechuic acid, and trans-p-coumaryl alcohol. HPLC fingerprinting demonstrated high batch-to-batch consistency, while quantitative analysis determined a trans-p-coumaryl alcohol content of 8.77 mg/g extract. Critically, biological evaluation showed that WCR exhibited potent anti-inflammatory activity by inhibiting nitric oxide production, with a superior inhibitory effect compared with the reference drug indomethacin. This study provides a preliminary scientific framework for the standardization of WCR. It defines precise quality specifications and a potential bioactive marker, establishing the rigor needed for regulatory certification and industrial production. This work connects traditional Thai medicine with evidence-based pharmaceutics, positioning WCR as a promising therapy for psoriasis.