The protective effects of Trichoderma asperellum IZR D-11 as a biocontrol agent against the powdery mildew Erysiphe alphitoides infecting leaves of Quercus robur were evaluated for the first time. A strain of Trichoderma had earlier been isolated in Belarus, and was identified in this study as T. asperellum by sequencing of three genomic markers: internal transcribed spacer, translation elongation factor 1 alpha and RNA polymerase II subunit 2, with over 99.2% identity to corresponding genomic sequences in GenBank. When applied once in the first year just after onset of powdery mildew disease, T asperellum IZR D-11 reduced disease progression and continued to reduce powdery mildew levels during the following three years. Photosynthetic activity as represented by chlorophyll fluorescence measured in oak seedlings was increased in treated plants, and greater assimilate production was also found. The use of this antagonistic fungus increased the total water content in oak leaves suggesting that T. asperellum IZR D-11 can serve as a preventive measure to reduce energy losses in the process of water transpiration. GC-MS analysis detected 49 volatile compounds in the headspace of pure cultures of T. asperellum. Sesquiterpenes represented mainly by daucene, dauca-4(11),8-diene and isodaucene were the largest group of compounds emitted. We speculate that these volatiles from T. asperellum IZR D-11 may be involved in induced resistance in the plant, but further research is needed. The above results suggest that T. asperellum strain IZR D-11 has potential as a biocontrol agent of oak powdery mildew in forest nurseries.

In this work, the effect of a new generation biopreparation (btmstr-biopreparation) based on the metabolites of the fungus Trichoderma harzianum and the bacterium Bacillus thuringiensis on the yield of mungbean plant was studied. According to the results of btmstr-biopreparation, prepared from the culture fluid of the Trichoderma harzianum sp.76 strain synthesizing ISC, as well as from a mutant strain of the bacterium Bacillus thuringiensis var. thuringiensis synthesizing melanin, showed the possibility of obtaining a harvest 77.4% more than the control variant (Btnms biopreparation), or 61.4% more compared to the template an option. During the research, it was found that under production conditions, various aphids, mustache, candela, grass moth, leaf beetles and fungi causing fusarium, as well as microbial objects causing bacterial spotting occur on the mungbean plant. Therefore, this biopreparation is recommended for use to increase the yield of agricultural plants and combat various diseases and pests.

Entomopathogenic nematodes (EPNs) are crucial biocontrol agents, yet optimizing the yield and quality of infective juveniles (IJs) during commercial liquid production remains challenging. This study utilized a central composite rotatable design to optimize liquid culture parameters (ascaroside, dimethyl sulfoxide, medium volume, IJ inocula) for Heterorhabditis bacteriophora H06 and Steinernema carpocapsae All. The results demonstrated that improving aeration (inferred from reduced media volume), combined with ascr#11 regulation, synergistically enhanced IJ yield and quality. Under optimized conditions, yields reached 3.35 × 10⁵ IJs/mL for H. bacteriophora H06 and 2.67 × 10⁵ IJs/mL for S. carpocapsae All. Crucially, the IJs from the high-yield flask exhibited significantly superior infectivity (24–26% single-IJ infection rate) compared to solid-culture controls (13–14%). Targeted metabolomics profiling of sugar, energy and fatty acids of H. bacteriophora H06 revealed upregulated tricarboxylic acid (TCA) cycle intermediates (citrate, pyruvate) and the significant accumulation of stress-protectant trehalose and immune-modulating polyunsaturated fatty acids (eicosapentaenoic acid, arachidonic acid). These findings establish a fermentation strategy that simultaneously enhances IJ yield and biological quality by reducing media volume (used as a proxy for improved aeration) and supplementing ascr#11. Furthermore, the distinct metabolic profile enriched in energy, stress, and immune-modulating metabolites identified in H. bacteriophora provides a plausible explanatory framework for the parallel phenotypic improvements observed across both species.

The Republic of Uzbekistan is a country specializing in agriculture, with favorable climatic conditions that allow high yields of almost all types of crops.At the moment, in our republic, 1 million 387 thousand hectares of land is used for growing cotton, about 1 million 700 thousand hectares of grain products (including 1 million 200 thousand hectares of irrigated areas), more than 560 thousand hectares of fruits and vegetables and products horticulture, and 967.8 1000 hectares are specialized in growing fodder plants. In the absence of large losses in the cultivation and storage of agricultural products, this amount of land would be enough to freely provide the population of the republic with food and technical raw materials and export some of the products. This article provides analytical information about the work carried out on the basis of the entomopathogenic bacterium Bacillus thuringiensis in the protection of agricultural crops from harmful insects. Information is also provided on the production of b-exotoxin, L-exotoxin, a-exotoxin, U-exotoxin, crystalline d-endotoxin and their classification.

ЭФФЕКТИВНОСТЬ ПРИМЕНЕНИЯ БИОЛОГИЧЕСКИХ ПРЕПАРАТОВ В АГРОЦЕНОЗЕ КОРМОВЫХ БОБОВ В БЕЛАРУСИ

The aim of this research is the monitoring of diseases of Scots pine in the plantings and forest stands in the Republic of Belarus depending on the species composition of pests, their biology and the effectiveness of various methods of plant protection. Significant loss of plants for planting is associated with the spread of pathogens causing damage to seedlings, mainly from the genus Fusarium, Alternaria and Botrytis. The most abundant pests of Scots pine are Coleoptera and Lepidoptera groups representing 16 and 8 species, accordingly. Numerous species represent Curculionidae, Scarabaeidae and Tortricidae family. The main components of an effective system of measures, as an integral part of the technology of growing of planting material of Scots pine in containers, are pre-sowing seed treatment with fungicides, stimulating the growth and development of seedlings. The elaborated system of protective measures of forest plantations is based on the prevalence, phenology of pests, estimation of phytopathogenic load, monitoring of diseases and seasonal climatic conditions.