Shock buffet is one of the critical issues affecting the aerodynamic performance, flight quality, and flight safety of large aircraft. To overcome the limitations of traditional experimental measurement methods, such as insufficient capability in capturing flow features and high cost, an integrated experimental system tailored for extreme cryogenic and high-Reynolds-number conditions is developed based on the conventional tuft technique. This system comprises “preparation of low-flow-disturbance fluorescent mini-tufts, high-efficiency large-area tuft taping, automatic generation of digital streamline, and flow topology analysis”. Furthermore, a technique for assessing the transonic shock buffet onset using dynamic flow visualization with fluorescent mini-tufts is proposed. This paper takes a typical supercritical airfoil as the research object. First, through high-precision numerical simulations, it reveals that low-energy, unstable boundary-layer separation is the core driving force for the development and maintenance of shock buffet, and that flow separation characteristics serve as an important basis for determining the shock buffet onset. Subsequently, experimental validation is conducted in a 0.3 m high-Reynolds-number transonic wind tunnel. Using a dual-excitation-band composite light source, simultaneous measurements of pressure-sensitive paint (PSP) and fluorescent mini-tuft patterns are realized. The experimental results show that under extreme conditions, characterized by a wide total temperature range of 110 K to 280 K and strong scouring at Mach numbers from 0.6 to 0.9, the fluorescent mini-tufts (approximately 0.05 mm in diameter) exhibit excellent flow-following capability without any detachment. The digitized flow patterns of the fluorescent mini-tufts, obtained via computer image recognition algorithms, clearly reveal the location and area of boundary-layer separation. The trends show good agreement with the cryogenic PSP results, providing an important reference for determining the shock buffet onset.