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Maslinic acid (MA) is a pentacyclic triterpene abundant in olive peels. MA reportedly increases skeletal muscle mass and strength in older adults; however, the underlying mechanism is unknown. This study aimed to investigate the effects of MA on denervated muscle atrophy and strength and to explore the underlying molecular mechanism. Mice were fed either a control diet or a 0.27% MA diet. One week after intervention, the sciatic nerves of both legs were cut to induce muscle atrophy. Mice were examined 14 days after denervation. MA prevented the denervation-induced reduction in gastrocnemius muscle mass and skeletal muscle strength. Microarray gene expression profiling in gastrocnemius muscle demonstrated several potential mechanisms for muscle maintenance. Gene set enrichment analysis (GSEA) revealed different enriched biological processes, such as myogenesis, PI3/AKT/mTOR signaling, TNFα signaling via NF-κB, and TGF-β signaling in MA-treated mice. In addition, qPCR data showed that MA induced Igf1 expression and suppressed the expressions of Atrogin-1,Murf1 and Tgfb. Altogether, our results suggest the potential of MA as a new therapeutic and preventive dietary ingredient for muscular atrophy and strength.
Yuki Yamauchi; Farhana Ferdousi; Satoshi Fukumitsu; Hiroko Isoda. Maslinic Acid Attenuates Denervation-Induced Loss of Skeletal Muscle Mass and Strength. Nutrients 2021, 13, 2950 .
AMA StyleYuki Yamauchi, Farhana Ferdousi, Satoshi Fukumitsu, Hiroko Isoda. Maslinic Acid Attenuates Denervation-Induced Loss of Skeletal Muscle Mass and Strength. Nutrients. 2021; 13 (9):2950.
Chicago/Turabian StyleYuki Yamauchi; Farhana Ferdousi; Satoshi Fukumitsu; Hiroko Isoda. 2021. "Maslinic Acid Attenuates Denervation-Induced Loss of Skeletal Muscle Mass and Strength." Nutrients 13, no. 9: 2950.
The beneficial effect on health of argan oil is recognized worldwide. We have previously reported that the cake that remains after argan oil extraction (argan press-cake or APC) inhibits melanogenesis in B16 melanoma cells in a time-dependent manner without cytotoxicity. In this study, the global gene expression profile of B16 melanoma cells treated with APC extract was determined in order to gain an understanding of the possible mechanisms of action of APC. The results suggest that APC extract inhibits melanin biosynthesis by down-regulating microphthalmia-associated transcription factor (Mitf) and its downstream signaling pathway through JNK signaling activation, and the inhibition of Wnt/β-catenin and cAMP/PKA signaling pathways. APC extract also prevented the transport of melanosomes by down-regulating Rab27a expression. These results suggest that APC may be an important natural skin whitening product and pharmacological agent used for clinical treatment of pigmentary disorders.
Thouria Bourhim; Myra O. Villareal; Chemseddoha Gadhi; Hiroko Isoda. Elucidation of Melanogenesis-Associated Signaling Pathways Regulated by Argan Press Cake in B16 Melanoma Cells. Nutrients 2021, 13, 2697 .
AMA StyleThouria Bourhim, Myra O. Villareal, Chemseddoha Gadhi, Hiroko Isoda. Elucidation of Melanogenesis-Associated Signaling Pathways Regulated by Argan Press Cake in B16 Melanoma Cells. Nutrients. 2021; 13 (8):2697.
Chicago/Turabian StyleThouria Bourhim; Myra O. Villareal; Chemseddoha Gadhi; Hiroko Isoda. 2021. "Elucidation of Melanogenesis-Associated Signaling Pathways Regulated by Argan Press Cake in B16 Melanoma Cells." Nutrients 13, no. 8: 2697.
A comparative study of bioactive lipids extraction from black cumin (Nigella sativa L.) and basil (Ocimum basilicum L.) seeds using conventional petroleum-based solvent and green solvent 2-methyltetrahydrofuran (MeTHF) was performed. MeTHF extraction allowed obtaining the highest oil yield in black cumin (34%). Regarding fatty acids composition, linoleic acid (61%) and α-linolenic (78%) were relevant in black cumin and basil green and conventionally extracted oils, respectively. Besides, MeTHF allowed obtaining higher tocopherols and total phenolics contents in black cumin (400 mg/kg of oil and 12 mg EGA/g oil) and basil (317 mg/kg oil and 5 mg EGA/g oil) compared to hexane-extracted ones. The content of major phenolic compounds in the two seed oils, trans-hydroxycinnamic acid, rosmarinic acid, and thymol was enhanced by MeTHF extraction. Furthermore, MeTHF-extracted oils possess stronger antioxidant activities (radical scavenging, total antioxidant, and β-carotene bleaching activities) and high and similar anti-inflammatory capacity to hexane-extracted oils. In conclusion, the results revealed that MeTHF is efficient to replace hazardous solvents to extract oil from black cumin and basil seeds rich in compounds relevant to the human diet, including essential polyunsaturated fatty acids (n-6 and n-3), tocopherols, and phenolic compounds with improved biological activities.
Soumaya Bourgou; Iness Bettaieb Rebey; Sofiene Ben Kaab; Majdi Hammami; Sarra Dakhlaoui; Selmi Sawsen; Kamel Msaada; Hiroko Isoda; Riadh Ksouri; Marie-Laure Fauconnier. Green Solvent to Substitute Hexane for Bioactive Lipids Extraction from Black Cumin and Basil Seeds. Foods 2021, 10, 1493 .
AMA StyleSoumaya Bourgou, Iness Bettaieb Rebey, Sofiene Ben Kaab, Majdi Hammami, Sarra Dakhlaoui, Selmi Sawsen, Kamel Msaada, Hiroko Isoda, Riadh Ksouri, Marie-Laure Fauconnier. Green Solvent to Substitute Hexane for Bioactive Lipids Extraction from Black Cumin and Basil Seeds. Foods. 2021; 10 (7):1493.
Chicago/Turabian StyleSoumaya Bourgou; Iness Bettaieb Rebey; Sofiene Ben Kaab; Majdi Hammami; Sarra Dakhlaoui; Selmi Sawsen; Kamel Msaada; Hiroko Isoda; Riadh Ksouri; Marie-Laure Fauconnier. 2021. "Green Solvent to Substitute Hexane for Bioactive Lipids Extraction from Black Cumin and Basil Seeds." Foods 10, no. 7: 1493.
Anaemia is one of the leading causes of disability in young adults and is associated with increased morbidity and mortality in elderly. With a global target to reduce the disease burden of anaemia, recent researches focus on novel compounds with the ability to induce erythropoiesis and regulate iron homeostasis. We aimed to explore the biological events and potential polypharmacological effects of water-extracted olive leaf (WOL) on human bone marrow–derived haematopoietic stem cells (hHSCs) using a comprehensive gene expression analysis. HPLC analysis identifies six bioactive polyphenols in the WOL. Treatment with WOL for 12 days regulated gene expressions related to erythroid differentiation, oxygen homeostasis, iron homeostasis, haem metabolism and Hb biosynthesis in hHSCs. Functional clustering analysis reveals several major functions of WOL such as ribosomal biogenesis and mitochondrial translation machinery, glycolytic process, ATP biosynthesis and immune response. Additionally, the colonies of both primitive and mature erythroid progenitors, CFU-E and BFU-E, were significantly increased in WOL-treated hHSCs. The expressions of erythroid markers, CD47, glycophorin A (GYPA), and transferrin receptor (TFRC) and adult Hb subunits-HBA and HBB were also confirmed in immunofluorescent staining and flow cytometer analysis in WOL-treated hHSCs. It is well known that induction of lineage-specific differentiation, as well as the maturation of early haematopoietic precursors into fully mature erythrocytes, involves multiple simultaneous biological events and complex signalling networks. In this regard, our genome-wide transcriptome profiling with microarray study on WOL-treated hHSCs provides general insights into the multitarget prophylactic and/or therapeutic potential of WOL in anaemia and other haematological disorders.
Shinji Kondo; Farhana Ferdousi; Ken Yamauchi; Sofya Suidasari; Miki Yokozawa; Mohamed Moncef Harrabi; Ken‐Ichi Tominaga; Hiroko Isoda. Comprehensive transcriptome analysis of erythroid differentiation potential of olive leaf in haematopoietic stem cells. Journal of Cellular and Molecular Medicine 2021, 25, 7229 -7243.
AMA StyleShinji Kondo, Farhana Ferdousi, Ken Yamauchi, Sofya Suidasari, Miki Yokozawa, Mohamed Moncef Harrabi, Ken‐Ichi Tominaga, Hiroko Isoda. Comprehensive transcriptome analysis of erythroid differentiation potential of olive leaf in haematopoietic stem cells. Journal of Cellular and Molecular Medicine. 2021; 25 (15):7229-7243.
Chicago/Turabian StyleShinji Kondo; Farhana Ferdousi; Ken Yamauchi; Sofya Suidasari; Miki Yokozawa; Mohamed Moncef Harrabi; Ken‐Ichi Tominaga; Hiroko Isoda. 2021. "Comprehensive transcriptome analysis of erythroid differentiation potential of olive leaf in haematopoietic stem cells." Journal of Cellular and Molecular Medicine 25, no. 15: 7229-7243.
The effectiveness of anthocyanins may differ according to their chemical structures; however, randomized clinical controlled trials (RCTs) or meta-analyses that examine the consequences of these structural differences have not been reported yet. In this meta-analysis, anthocyanins in test foods of 18 selected RCTs were categorized into three types: cyanidin-, delphinidin-, and malvidin-based. Delphinidin-based anthocyanins demonstrated significant effects on triglycerides (mean difference (MD): −0.24, p< 0.01), low-density lipoprotein cholesterol (LDL-C) (MD: −0.28, p< 0.001), and high-density lipoprotein cholesterol (HDL-C) (MD: 0.11, p< 0.01), whereas no significant effects were observed for cyanidin- and malvidin-based anthocyanins. Although non-significant, favorable effects on total cholesterol (TC) and HDL-C were observed for cyanidin- and malvidin-based anthocyanins, respectively (both p< 0.1). The ascending order of effectiveness on TC and LDL-C was delphinidin-, cyanidin-, and malvidin-based anthocyanins, and the differences among the three groups were significant (both p< 0.05). We could not confirm the significant effects of each main anthocyanin on glucose metabolism; however, insulin resistance index changed positively and negatively with cyanidin- and delphinidin-based anthocyanins, respectively. Therefore, foods containing mainly unmethylated anthocyanins, especially with large numbers of OH groups, may improve glucose and lipid metabolism more effectively than those containing methylated anthocyanins.
Risa Araki; Akira Yada; Hirotsugu Ueda; Kenichi Tominaga; Hiroko Isoda. Differences in the Effects of Anthocyanin Supplementation on Glucose and Lipid Metabolism According to the Structure of the Main Anthocyanin: A Meta-Analysis of Randomized Controlled Trials. Nutrients 2021, 13, 2003 .
AMA StyleRisa Araki, Akira Yada, Hirotsugu Ueda, Kenichi Tominaga, Hiroko Isoda. Differences in the Effects of Anthocyanin Supplementation on Glucose and Lipid Metabolism According to the Structure of the Main Anthocyanin: A Meta-Analysis of Randomized Controlled Trials. Nutrients. 2021; 13 (6):2003.
Chicago/Turabian StyleRisa Araki; Akira Yada; Hirotsugu Ueda; Kenichi Tominaga; Hiroko Isoda. 2021. "Differences in the Effects of Anthocyanin Supplementation on Glucose and Lipid Metabolism According to the Structure of the Main Anthocyanin: A Meta-Analysis of Randomized Controlled Trials." Nutrients 13, no. 6: 2003.
Background Hair follicle undergoes a growth cycle under the regulation of dermal papilla cells. Due to their enormous roles, these fibroblast cells have been used in various in vitro studies as a screening model to evaluate the effect of hair growth regulating agents. Objective In the current study, we aim to check the hair growth potential effect of Argan press cake (APC) extracted using 50 or 80 % aqueous ethanol on human hair follicle dermal papilla cells (HFDPCs) and to determine the molecular mechanism. Methods APC were applied to HFDPCs, then cell proliferation assays, mitochondrial biogenesis assay, and oxidative stress assay were assessed. DNA microarray was performed from the cells treated with our samples and minoxidil. Validation of the results was done using Quantitative Real-Time PCR with primers for hair-growth related genes. GC/MS analysis was used to determine the compounds contained in APC 50 and 80 %. Results APC enhanced cell proliferation along with the stimulation of the ATP content. Additionally, APC had an anti-oxidant activity against H2O2 mediated oxidative stress preventing dermal papilla cell senescence. Consistent with this, global gene profiling analysis showed an activation of hair growth-related pathway, and a downregulation of inflammation- and oxidative stress-related genes by APC extracts. GC/MS analysis revealed that these extracts contained pure fatty acids, derived sugar chains, and pure compounds including tocopherols, squalene, and spinasterol. Conclusion Taken together, here we showed that APC extracts had an effect on stimulating hair growth while inhibiting the inflammation and the oxidative stress of HFDPCs and thus can potentially contribute to an anti-hair loss drug development.
Meriem Bejaoui; Noamane Taarji; Masako Saito; Mitsutoshi Nakajima; Hiroko Isoda. Argan (Argania Spinosa) press cake extract enhances cell proliferation and prevents oxidative stress and inflammation of human dermal papilla cells. Journal of Dermatological Science 2021, 103, 33 -40.
AMA StyleMeriem Bejaoui, Noamane Taarji, Masako Saito, Mitsutoshi Nakajima, Hiroko Isoda. Argan (Argania Spinosa) press cake extract enhances cell proliferation and prevents oxidative stress and inflammation of human dermal papilla cells. Journal of Dermatological Science. 2021; 103 (1):33-40.
Chicago/Turabian StyleMeriem Bejaoui; Noamane Taarji; Masako Saito; Mitsutoshi Nakajima; Hiroko Isoda. 2021. "Argan (Argania Spinosa) press cake extract enhances cell proliferation and prevents oxidative stress and inflammation of human dermal papilla cells." Journal of Dermatological Science 103, no. 1: 33-40.
The 70% ethanol extract of Artemisia campestris was screened to find PPARγ ligands using the PPARγ ligand-responsive chimera luciferase reporter system. Capillartemisin B was identified as a PPARγ ligand that stimulated lipid accumulation in 3T3-L1 cells. By further purification of PPARγ ligands from a large-scale preparation of the methanol extract of Artemisia campestris, we isolated and identified eupatilin and santaflavone as PPARγ ligands. Weak PPARγ ligand activity of eupatilin or santaflavone in reporter assay was enhanced by a PPARγ antagonist, GW9662, suggesting that santaflavone or eupatilin and GW9662 bound simultaneously to the multiple sub-pockets of the PPARγ ligand-binding domain (LBD) and cooperatively activated PPARγ. Docking simulation suggested that eupatilin binds to the Ω-pocket but not to the AF-2 pocket of Y-shaped PPARγ LBD where artepillin C that differs from capillartemisin B at the C-5′ position without hydroxy group binds. Eupatilin or santaflavone with or without GW9662 did not stimulate lipid accumulation in differentiated 3T3-L1 cells, suggesting that binding of each compound alone or with GW9662 to the Ω-pocket which stimulated the PPARγ-responsive reporter expression was not enough to stimulate lipid accumulation. The PPARγ ligands found in this study have a potential to design the fragment-based drug design of a novel PPARγ ligand that cover the Y-shaped PPARγ LBD.
Tokio Hasegawa; Mayo Osaka; Yusaku Miyamae; Katsutoshi Nishino; Hiroko Isoda; Kiyokazu Kawada; Mohamed Neffati; Kazuhiro Irie; Masaya Nagao. Two Types of PPARγ Ligands Identified in the Extract of Artemisia campestris. Chemistry 2021, 3, 647 -657.
AMA StyleTokio Hasegawa, Mayo Osaka, Yusaku Miyamae, Katsutoshi Nishino, Hiroko Isoda, Kiyokazu Kawada, Mohamed Neffati, Kazuhiro Irie, Masaya Nagao. Two Types of PPARγ Ligands Identified in the Extract of Artemisia campestris. Chemistry. 2021; 3 (2):647-657.
Chicago/Turabian StyleTokio Hasegawa; Mayo Osaka; Yusaku Miyamae; Katsutoshi Nishino; Hiroko Isoda; Kiyokazu Kawada; Mohamed Neffati; Kazuhiro Irie; Masaya Nagao. 2021. "Two Types of PPARγ Ligands Identified in the Extract of Artemisia campestris." Chemistry 3, no. 2: 647-657.
Cyanidin, a kind of anthocyanin, has been reported to have chemotherapeutic activities in humans. Human amniotic epithelial cells (hAECs) are considered a potential source of pluripotent stem cells. hAECs have been used as a novel tool in regenerative cellular therapy and cell differentiation studies. In this study, to explore the effects of cyanidin-3-O-glucoside (Cy3G) on hAECs and their mechanisms, we investigated the transcriptomic changes in the Cy3G-treated cells using microarray analysis. Among the differentially expressed genes (Fold change > 1.1; p-value < 0.05), 109 genes were upregulated and 232 were downregulated. Ratios of upregulated and downregulated genes were 0.22% and 0.47% of the total expressed genes, respectively. Next, we explored the enriched gene ontology, i.e., the biological process, molecular function, and cellular component of the 37 upregulated (>1.3-fold change) and 124 downregulated (<1.3-fold change) genes. Significantly enriched biological processes by the upregulated genes included “response to muscle activity,” and the genes involved in this gene ontology (GO) were Metrnl and SRD5A1, which function in the adipocyte. On the other hand, the cell cycle biological process was significantly enriched by the downregulated genes, including some from the SMC gene family. An adipogenesis-associated gene DDX6 was also included in the cell cycle biological process. Thus, our findings suggest the prospects of Cy3G in modulating adipocyte differentiation in hAECs.
Shinya Takahashi; Farhana Ferdousi; Yun-Wen Zheng; Tatsuya Oda; Hiroko Isoda. Human Amniotic Epithelial Cells as a Tool to Investigate the Effects of Cyanidin 3-O-Glucoside on Cell Differentiation. International Journal of Molecular Sciences 2021, 22, 3768 .
AMA StyleShinya Takahashi, Farhana Ferdousi, Yun-Wen Zheng, Tatsuya Oda, Hiroko Isoda. Human Amniotic Epithelial Cells as a Tool to Investigate the Effects of Cyanidin 3-O-Glucoside on Cell Differentiation. International Journal of Molecular Sciences. 2021; 22 (7):3768.
Chicago/Turabian StyleShinya Takahashi; Farhana Ferdousi; Yun-Wen Zheng; Tatsuya Oda; Hiroko Isoda. 2021. "Human Amniotic Epithelial Cells as a Tool to Investigate the Effects of Cyanidin 3-O-Glucoside on Cell Differentiation." International Journal of Molecular Sciences 22, no. 7: 3768.
The surface-active and emulsifying properties of crude aqueous ethanolic extracts from untreated olive oil cake (OOC) were investigated. OOC extracts contained important concentrations of surface-active components including proteins, saponins and polyphenols (1.2–2.8%, 7.8–9.5% and 0.7–4.5% (w/w), respectively) and reduced the interfacial tension by up to 46% (14.0 ± 0.2 mN m−1) at the oil–water interface. The emulsifying ability of OOC extracts was not correlated, however, with their interfacial activity or surface-active composition. Eighty percent aqueous ethanol extract produced the most stable oil-in-water (O/W) emulsions by high-pressure homogenization. The emulsions had average volume mean droplet diameters of approximately 0.4 µm and negative ζ-potentials of about −45 mV, and were stable for up to 1 month of storage at 5, 25 and 50 °C. They were sensitive, however, to acidic pH conditions (<5) and NaCl addition (≥25 mM), indicating that the main stabilization mechanism is electrostatic due to the presence of surface-active compounds with ionizable groups, such as saponins.
Firdaous Fainassi; Noamane Taarji; Fatiha Benkhalti; Abdellatif Hafidi; Marcos Neves; Hiroko Isoda; Mitsutoshi Nakajima. Emulsion Formation and Stabilizing Properties of Olive Oil Cake Crude Extracts. Processes 2021, 9, 633 .
AMA StyleFirdaous Fainassi, Noamane Taarji, Fatiha Benkhalti, Abdellatif Hafidi, Marcos Neves, Hiroko Isoda, Mitsutoshi Nakajima. Emulsion Formation and Stabilizing Properties of Olive Oil Cake Crude Extracts. Processes. 2021; 9 (4):633.
Chicago/Turabian StyleFirdaous Fainassi; Noamane Taarji; Fatiha Benkhalti; Abdellatif Hafidi; Marcos Neves; Hiroko Isoda; Mitsutoshi Nakajima. 2021. "Emulsion Formation and Stabilizing Properties of Olive Oil Cake Crude Extracts." Processes 9, no. 4: 633.
This study identified anserine and anserine/carnosine in chicken breast of Thai native chicken (TNC; 100% Thai native), Thai synthetic chicken (TSC; 50% Thai native), and Thai native crossbred chicken (TNC crossbred; 25% Thai native) compared with commercial broiler chicken (BR; 0% Thai native) using nuclear magnetic resonance (NMR) spectroscopy and the effect on antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl assay (DPPH). We conducted experiments with a completely randomized design and explored principal components analysis (PCA) and orthogonal projection to latent structure-discriminant analysis (OPLS-DA) to identify the distinguishing metabolites and relative concentrations from 1H NMR spectra among the groups. The relative concentrations and antioxidant properties among the groups were analyzed by analysis of variance (ANOVA) using the general linear model (GLM). This study revealed seven metabolites alanine, inositol monophosphate (IMP), inosine, and anserine/carnosine, lactate, anserine, and creatine. Lactate, anserine, and creatine were major components. In terms of PCA, the plots can distinguish BR from other groups. OPLS-DA revealed that anserine and anserine/carnosine in the chicken breast were significantly higher in TNC, TSC, and TNC crossbred than BR according to their relative concentrations and antioxidant properties (p < 0.01). Therefore, TNCs and their crossbreeds might have the potential to be functional meat sources.
Sukanya Charoensin; Banyat Laopaiboon; Wuttigrai Boonkum; Jutarop Phetcharaburanin; Myra Villareal; Hiroko Isoda; Monchai Duangjinda. Thai Native Chicken as a Potential Functional Meat Source Rich in Anserine, Anserine/Carnosine, and Antioxidant Substances. Animals 2021, 11, 902 .
AMA StyleSukanya Charoensin, Banyat Laopaiboon, Wuttigrai Boonkum, Jutarop Phetcharaburanin, Myra Villareal, Hiroko Isoda, Monchai Duangjinda. Thai Native Chicken as a Potential Functional Meat Source Rich in Anserine, Anserine/Carnosine, and Antioxidant Substances. Animals. 2021; 11 (3):902.
Chicago/Turabian StyleSukanya Charoensin; Banyat Laopaiboon; Wuttigrai Boonkum; Jutarop Phetcharaburanin; Myra Villareal; Hiroko Isoda; Monchai Duangjinda. 2021. "Thai Native Chicken as a Potential Functional Meat Source Rich in Anserine, Anserine/Carnosine, and Antioxidant Substances." Animals 11, no. 3: 902.
Over the past years, Human Amnion Epithelial Cells (hAECs), a placental stem cell, are gaining higher attention from the scientific community as they showed several advantages over other types of stem cells, including availability, easy accessibility, reduced rejection rate, non-tumorigenicity, and minimal legal constraint. Recently, natural compounds are used to stimulate stem cell differentiation and proliferation and to enhance their disease-treating potential. A polyphenolic compound 3,4,5-Tri-O-Caffeoylquinic Acid (TCQA) has been previously reported to induce human neural stem cell differentiation and may affect melanocyte stem cell differentiation as well. In this study, TCQA was tested on 3D cultured hAECs after seven days of treatment, and then, microarray gene expression profiling was conducted of TCQA-treated and untreated control cells on day 0 and day 7. Analyses revealed that TCQA treatment significantly enriched pigment and neural cells sets; besides, genes linked with neurogenesis, oxidation–reduction process, epidermal development, and metabolism were positively regulated. Interestingly, TCQA stimulated cell cycle arrest-related pathways and differentiation signaling. On the other hand, TCQA decreased interleukins and cytokines expression and this due to its anti-inflammatory properties as a polyphenolic compound. Results were validated to highlight the main activities of TCQA on hAECs, including differentiation, cell cycle arrest, and anti-inflammatory. This study highlights the important role of hAECs in regenerative medicine and the use of natural compounds to regulate their fate.
Meriem Bejaoui; Farhana Ferdousi; Yun-Wen Zheng; Tatsuya Oda; Hiroko Isoda. Regulating cell fate of human amnion epithelial cells using natural compounds: an example of enhanced neural and pigment differentiation by 3,4,5-tri-O-caffeoylquinic acid. Cell Communication and Signaling 2021, 19, 1 -12.
AMA StyleMeriem Bejaoui, Farhana Ferdousi, Yun-Wen Zheng, Tatsuya Oda, Hiroko Isoda. Regulating cell fate of human amnion epithelial cells using natural compounds: an example of enhanced neural and pigment differentiation by 3,4,5-tri-O-caffeoylquinic acid. Cell Communication and Signaling. 2021; 19 (1):1-12.
Chicago/Turabian StyleMeriem Bejaoui; Farhana Ferdousi; Yun-Wen Zheng; Tatsuya Oda; Hiroko Isoda. 2021. "Regulating cell fate of human amnion epithelial cells using natural compounds: an example of enhanced neural and pigment differentiation by 3,4,5-tri-O-caffeoylquinic acid." Cell Communication and Signaling 19, no. 1: 1-12.
Background: Rosmarinus officinalis L.is traditionally used as an infusion in the treatment of several diseases and in particular against neuropsychiatric disorders, such as anxiety and depression. It was established that rosemary extracts show an antidepressant effect on animal models. However, to the best of our knowledge, there is no scientific data that highlights the therapeutic effects of rosemary intake on human mental health. Aim:This study investigated whether rosemary tea consumption affects the plasma levels of anxiety and depression biomarkers in healthy volunteers. Methods:Twenty-two healthy volunteers aged between 20 and 50 years old consumed rosemary tea prepared from 5 g of dried rosemary in 100 mL boiled water once a day for 10 days. Plasma concentrations of Brain-Derived Neurotrophic Factor (BDNF), Interleukine-6 (IL-6), Interleukine-4 (IL-4), Tumor Necrosis Factor- alpha (TNF-α), Interferon-gamma (IFNϒ), and cortisol were measured by enzyme-linked immunosorbent assay using commercial ELISA kits (R&D systems) before rosemary consumption and at the end of the experiment. Results:Rosemary tea consumption significantly increased the concentration of BDNF([BDNF]D0 = 22363.86 ± 12987.66 pg/mL, [BDNF]D10 = 41803.64 ± 28109.19, p = 0.006) and TNF-α([TNF-α] D0 = 39.49 ± 14.44 pg/mL, [TNF-α] D10 = 56.24 ± 39.01, p = 0.016). However, a slight variation that was statistically non-significant in INFϒ, cortisol, IL-4, IL-6 levels and in the ratio IL-4/INFϒ was observed (p > 0.05). Conclusion:Our findings highlight the promising anxiolytic and/or antidepressant effects of rosemary tea consumption in healthy volunteers since it increases the level of the most reliable depression biomarker BDNF. However, more powerful studies with larger sample size, carefully-chosen target population and, an extended intervention period are required.
Mariem Achour; Intidhar Ben Salem; Farhana Ferdousi; Manel Nouira; Maha Ben Fredj; Ali Mtiraoui; Hiroko Isoda; Saad Saguem. Rosemary Tea Consumption Alters Peripheral Anxiety and Depression Biomarkers: A Pilot Study in Limited Healthy Volunteers. Journal of the American College of Nutrition 2021, 1 -10.
AMA StyleMariem Achour, Intidhar Ben Salem, Farhana Ferdousi, Manel Nouira, Maha Ben Fredj, Ali Mtiraoui, Hiroko Isoda, Saad Saguem. Rosemary Tea Consumption Alters Peripheral Anxiety and Depression Biomarkers: A Pilot Study in Limited Healthy Volunteers. Journal of the American College of Nutrition. 2021; ():1-10.
Chicago/Turabian StyleMariem Achour; Intidhar Ben Salem; Farhana Ferdousi; Manel Nouira; Maha Ben Fredj; Ali Mtiraoui; Hiroko Isoda; Saad Saguem. 2021. "Rosemary Tea Consumption Alters Peripheral Anxiety and Depression Biomarkers: A Pilot Study in Limited Healthy Volunteers." Journal of the American College of Nutrition , no. : 1-10.
Much attention has recently been focused on nutraceuticals, with minimal adverse effects, developed for preventing or treating neurological diseases such as Alzheimer's disease (AD). The present study was conducted to investigate the potential effect on neural development and function of the microalgae Aurantiochytrium sp. as a nutraceutical. To test neuroprotection by the ethanol extract of Aurantiochytrium (EEA) and a derivative, the n-Hexane layer of EEA (HEEA), amyloid-β-stimulated SH-SY5Y cells, was used as an in vitro AD model. We then assessed the potential enhancement of neurogenesis by EEA and HEEA using murine ex vivo neurospheres. We also administered EEA or HEEA to senescence-accelerated mouse-prone 8 (SAMP8) mice, a non-transgenic strain with accelerated aging and AD-like memory loss for evaluation of spatial learning and memory using the Morris water maze test. Finally, we performed immunohistochemical analysis for assessment of neurogenesis in mice administered EEA. Pretreatment of SH-SY5Y cells with EEA or the squalene-rich fraction of EEA, HEEA, ameliorated amyloid-β-induced cytotoxicity. Interestingly, only EEA-treated cells showed a significant increase in cell metabolism and intracellular adenosine triphosphate production. Moreover, EEA treatment significantly increased the number of neurospheres, whereas HEEA treatment significantly increased the number of β-III-tubulin+ young neurons and GFAP+ astrocytes. SAMP8 mice were given 50 mg/kg EEA or HEEA orally for 30 days. EEA and HEEA decreased escape latency in the Morris water maze in SAMP8 mice, indicating improved memory. To detect stem cells and newborn neurons, we administered BrdU for 9 days and measured BrdU+ cells in the dentate gyrus, a neurogenic stem cell niche of the hippocampus. In SAMP8 mice, EEA rapidly and significantly increased the number of BrdU+GFAP+ stem cells and their progeny, BrdU+NeuN+ mature neurons. In conclusion, our data in aggregate indicate that EEA and its constituents could be developed into a nutraceutical for promoting brain health and function against several age-related diseases, particularly AD.
Kazunori Sasaki; Noelia Geribaldi-Doldán; Qingqing Wu; Julie Davies; Francis G. Szele; Hiroko Isoda. Microalgae Aurantiochytrium Sp. Increases Neurogenesis and Improves Spatial Learning and Memory in Senescence-Accelerated Mouse-Prone 8 Mice. Frontiers in Cell and Developmental Biology 2021, 8, 1 .
AMA StyleKazunori Sasaki, Noelia Geribaldi-Doldán, Qingqing Wu, Julie Davies, Francis G. Szele, Hiroko Isoda. Microalgae Aurantiochytrium Sp. Increases Neurogenesis and Improves Spatial Learning and Memory in Senescence-Accelerated Mouse-Prone 8 Mice. Frontiers in Cell and Developmental Biology. 2021; 8 ():1.
Chicago/Turabian StyleKazunori Sasaki; Noelia Geribaldi-Doldán; Qingqing Wu; Julie Davies; Francis G. Szele; Hiroko Isoda. 2021. "Microalgae Aurantiochytrium Sp. Increases Neurogenesis and Improves Spatial Learning and Memory in Senescence-Accelerated Mouse-Prone 8 Mice." Frontiers in Cell and Developmental Biology 8, no. : 1.
Cuminaldehyde (CA) is one of the major compounds of the essential oil of Cuminum cyminum. The aim of this study was to evaluate the effects of CA on aging, specifically on spatial learning and memory. To achieve our objective, an in vitro study on SH-SY5Y cells was performed to analyze the neuroprotective effect of CA against dexamethasone using the MTT assay. An in vivo study was performed for evaluation of the spatial learning and memory using Morris water maze (MWM). RT-PCR was performed to quantify the expression of specific genes (Bdnf, Icam and ApoE) in the mice brain. The results obtained showed a neuroprotective effect of CA against dexamethasone-induced neuronal toxicity. The escape latency of CA-treated aged mice was significantly decreased as compared to the water-treated aged mice after 4 days of training in MWM. Moreover, CA treatment up-regulated the gene expression of Bdnf, Icam and ApoE, while it down-regulated the gene expression of IL-6. These findings suggest that CA has a neuroprotective effect, as well as a spatial learning and memory enhancement potential through the modulation of genes coding for neurotrophic factors and/or those implicated in the imbalance of neural circuitry and impairment of synaptic plasticity.
Zineb Omari; Sasaki Kazunori; Mouad Sabti; Meriem Bejaoui; Abdellatif Hafidi; Chemseddoha Gadhi; Hiroko Isoda. Dietary administration of cumin-derived cuminaldehyde induce neuroprotective and learning and memory enhancement effects to aging mice. Aging 2021, 13, 1671 -1685.
AMA StyleZineb Omari, Sasaki Kazunori, Mouad Sabti, Meriem Bejaoui, Abdellatif Hafidi, Chemseddoha Gadhi, Hiroko Isoda. Dietary administration of cumin-derived cuminaldehyde induce neuroprotective and learning and memory enhancement effects to aging mice. Aging. 2021; 13 (2):1671-1685.
Chicago/Turabian StyleZineb Omari; Sasaki Kazunori; Mouad Sabti; Meriem Bejaoui; Abdellatif Hafidi; Chemseddoha Gadhi; Hiroko Isoda. 2021. "Dietary administration of cumin-derived cuminaldehyde induce neuroprotective and learning and memory enhancement effects to aging mice." Aging 13, no. 2: 1671-1685.
The use of natural products for the regulation of skin pigmentation is gaining popularity. In the present study, we evaluated the effect of argan leaves extract (ALE) on melanogenesis in B16 melanoma cells, determined its antioxidant activity, then quantified and identified its phenolic components. B16 cells were treated with various concentrations of ALE, then the cell viability and proliferation were assessed using MTT assay while the melanin content was determined using spectrophotometric methods. The expression level of tyrosinase (TYR), tyrosinase related protein-1 (TRP-1) and dopachrome tautomerase (DCT) was evaluated by Western blotting. The antioxidant activity of ALE was investigated using four different assays while UPLC-ESI-HRMS analysis was used to characterize the ALE phenolic profile. Fourteen phenolic compounds were identified, of which six are reported for the first time to be present in ALE. ALE treatment increases the melanin content of B16 cells in a dose-dependent manner without cytotoxicity. This was revealed by the observed ALE-increased expression level of TYR, DCT, and TRP-1. These bioactivities may be mainly attributed to its high flavonoids content. Argan leaves have the potential for use as a treatment for hypopigmentation disorders and as a bioactive component of cosmetic products that aim to increase pigmentation.
Thouria Bourhim; Myra O. Villareal; François Couderc; Abdellatif Hafidi; Hiroko Isoda; Chemseddoha Gadhi. Melanogenesis Promoting Effect, Antioxidant Activity, and UPLC-ESI-HRMS Characterization of Phenolic Compounds of Argan Leaves Extract. Molecules 2021, 26, 371 .
AMA StyleThouria Bourhim, Myra O. Villareal, François Couderc, Abdellatif Hafidi, Hiroko Isoda, Chemseddoha Gadhi. Melanogenesis Promoting Effect, Antioxidant Activity, and UPLC-ESI-HRMS Characterization of Phenolic Compounds of Argan Leaves Extract. Molecules. 2021; 26 (2):371.
Chicago/Turabian StyleThouria Bourhim; Myra O. Villareal; François Couderc; Abdellatif Hafidi; Hiroko Isoda; Chemseddoha Gadhi. 2021. "Melanogenesis Promoting Effect, Antioxidant Activity, and UPLC-ESI-HRMS Characterization of Phenolic Compounds of Argan Leaves Extract." Molecules 26, no. 2: 371.
We previously performed a 4 week interventional trial that suggested that continuous intake of rosemary extract improves the mood states, fatigue, and cognitive function of working generation healthy adult Japanese men. However, the severity of depression in participants in our previous study was relatively mild. Therefore, in the present study, a post-hoc analysis of our previous study was conducted, limited to participants whose total mood disturbance (TMD) scores, which indicate greater mood disturbance, were above the median at baseline, to evaluate whether rosemary extract was effective for individuals with poor mental health. Following the intervention, the scores of TMD and “Confusion-Bewilderment” were significantly decreased (both p < 0.05), and scores of “Vigor-Activity” were significantly increased in the rosemary group (n = 8) compared with those in the control group (n = 13; p < 0.01). When comparing the scores from pre- and post-intervention, significant improvements in “Tension-Anxiety”, “Vigor-Activity”, “Fatigue on awakening”, “Daytime sleepiness”, and “Psychomotor speed” were observed in the rosemary group only (all p < 0.05). Based on these results, it was expected that rosemary extracts were effective for improving the mental energy and sleep quality of work-age men with poor mental health.
Risa Araki; Kazunori Sasaki; Hiroyuki Onda; Syunsuke Nakamura; Masahiro Kassai; Toshiyuki Kaneko; Hiroko Isoda; Koichi Hashimoto. Effects of Continuous Intake of Rosemary Extracts on Mental Health in Working Generation Healthy Japanese Men: Post-Hoc Testing of a Randomized Controlled Trial. Nutrients 2020, 12, 3551 .
AMA StyleRisa Araki, Kazunori Sasaki, Hiroyuki Onda, Syunsuke Nakamura, Masahiro Kassai, Toshiyuki Kaneko, Hiroko Isoda, Koichi Hashimoto. Effects of Continuous Intake of Rosemary Extracts on Mental Health in Working Generation Healthy Japanese Men: Post-Hoc Testing of a Randomized Controlled Trial. Nutrients. 2020; 12 (11):3551.
Chicago/Turabian StyleRisa Araki; Kazunori Sasaki; Hiroyuki Onda; Syunsuke Nakamura; Masahiro Kassai; Toshiyuki Kaneko; Hiroko Isoda; Koichi Hashimoto. 2020. "Effects of Continuous Intake of Rosemary Extracts on Mental Health in Working Generation Healthy Japanese Men: Post-Hoc Testing of a Randomized Controlled Trial." Nutrients 12, no. 11: 3551.
Human amnion epithelial cells (hAECs), derived from discarded term placenta, is anticipated as a new stem cell resource because of their advantages over embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), such as no risk of tumorigenicity and minimal ethical issue. hAECs have been reported to differentiate into hepatic-like cells (HLCs) with variable functionalities suitable for cell-based therapy of end-stage liver diseases, drug screening, and drug toxicity tests. On the other hand, a new research stream has been evolving to use natural compounds as stimulants of stem cell differentiation because of their high availability and minimum side effects. Isorhamnetin is a naturally occurring flavonoid commonly found in fruits and vegetables and has been reported to improve hepatic fibrosis and steatosis. In this present study, we have screened the differentiation potential of isorhamnetin in hAECs. The cells were grown on 3D cell culture and were treated with 20 μM of synthesized isorhamnetin for 10 days without adding any additional growth factors. DNA microarray global gene expression analysis was conducted for differentially expressed genes between isorhamnetin-treated and untreated control cells, gene expression validation was carried out using RT-qPCR method, and finally, several hepatic functions were assessed. Microarray analysis showed that isorhamnetin could activate essential biological processes, molecular functions, and signaling pathways for hepatic differentiation. Hepatic progenitor markers, EPCAM and DLK1, were upregulated in the isorhamnetin-treated hAECs. AFP was downregulated, while ALB was upregulated on Day 10. Furthermore, isorhamnetin-treated cells could show increased CYP enzyme mRNA levels, ICG uptake and release, glycogen storage activity, and urea secretion. Additionally, isorhamnetin-treated cells did not show any trace of transdifferentiation evident by significant downregulation of several colon- and cholangiocyte-specific markers. However, longer treatment with isorhamnetin did not promote hepatic maturation. Altogether, our findings indicate that isorhamnetin has a promising effect on directing the hepatic-lineage specific differentiation in hAECs.
Yoshiaki Uchida; Farhana Ferdousi; Yun-Wen Zheng; Tatsuya Oda; Hiroko Isoda. Global Gene Expression Profiling Reveals Isorhamnetin Induces Hepatic-Lineage Specific Differentiation in Human Amniotic Epithelial Cells. Frontiers in Cell and Developmental Biology 2020, 8, 1 .
AMA StyleYoshiaki Uchida, Farhana Ferdousi, Yun-Wen Zheng, Tatsuya Oda, Hiroko Isoda. Global Gene Expression Profiling Reveals Isorhamnetin Induces Hepatic-Lineage Specific Differentiation in Human Amniotic Epithelial Cells. Frontiers in Cell and Developmental Biology. 2020; 8 ():1.
Chicago/Turabian StyleYoshiaki Uchida; Farhana Ferdousi; Yun-Wen Zheng; Tatsuya Oda; Hiroko Isoda. 2020. "Global Gene Expression Profiling Reveals Isorhamnetin Induces Hepatic-Lineage Specific Differentiation in Human Amniotic Epithelial Cells." Frontiers in Cell and Developmental Biology 8, no. : 1.
Hyperuricemia is defined as a disease with high uric acid (UA) levels in the blood and a strong risk factor for gout. Urolithin A (UroA) is a main microbial metabolite derived from ellagic acid (EA), which occurs in strawberries and pomegranates. In this study, we evaluated antihyperuricemic effect of UroA in both cultured hepatocytes and hyperuricemic model mice. In cultured hepatocytes, UroA significantly and dose-dependently reduced UA production. In model mice with purine bodies-induced hyperuricemia, oral administration of UroA significantly inhibited the increase in plasma UA levels and hepatic xanthine oxidase (XO) activity. In addition, DNA microarray results exhibited that UroA, as well as allopurinol, a strong XO inhibitor, induced downregulation of the expression of genes associated with hepatic purine metabolism. Thus, hypouricemic effect of UroA could be, at least partly, attributed to inhibition of purine metabolism and UA production by suppressing XO activity in the liver. These results indicate UroA possesses a potent antihyperuricemic effect and it could be a potential candidate for a molecule capable of preventing and improving hyperuricemia and gout.
Shin-Ichi Adachi; Kazunori Sasaki; Shinji Kondo; Wataru Komatsu; Fumiaki Yoshizawa; Hiroko Isoda; Kazumi Yagasaki. Antihyperuricemic Effect of Urolithin A in Cultured Hepatocytes and Model Mice. Molecules 2020, 25, 5136 .
AMA StyleShin-Ichi Adachi, Kazunori Sasaki, Shinji Kondo, Wataru Komatsu, Fumiaki Yoshizawa, Hiroko Isoda, Kazumi Yagasaki. Antihyperuricemic Effect of Urolithin A in Cultured Hepatocytes and Model Mice. Molecules. 2020; 25 (21):5136.
Chicago/Turabian StyleShin-Ichi Adachi; Kazunori Sasaki; Shinji Kondo; Wataru Komatsu; Fumiaki Yoshizawa; Hiroko Isoda; Kazumi Yagasaki. 2020. "Antihyperuricemic Effect of Urolithin A in Cultured Hepatocytes and Model Mice." Molecules 25, no. 21: 5136.
Squalene (Sq) is a natural compound, found in various plant oils, algae, and larger quantity in deep-sea shark liver. It is also known as an intermediate of cholesterol synthesis in plants and animals including humans. Although evidences demonstrated its antioxidant, anticancer, hypolipidemic, and hepatoprotective and cardioprotective effects, its biological effects in cellular function might have been underestimated because of the water-insoluble property. To overcome this hydrophobicity, we synthesized new amphiphilic Sq derivative (HH-Sq). On the other hand, adipose-derived stem cells (ASCs) are a valuable source in regenerative medicine for its ease of accessibility and multilineage differentiation potential. Nevertheless, impaired cellular functions of ASCs derived from diabetic donor have still been debated controversially. In this study, we explored the effect of the HH-Sq in comparison to Sq on the adipocyte differentiation of ASCs obtained from subjects with type 2 diabetes. Gene expression profile by microarray analysis at 14 days of adipogenic differentiation revealed that HH-Sq induced more genes involved in intracellular signaling processes, whereas Sq activated more transmembrane receptor pathway-related genes. In addition, more important number of down-regulated and up-regulated genes by Sq and HH-Sq were not overlapped, suggesting the compounds might not only have difference in their chemical property but also potentially exert different biological effects. Both Sq and HH-Sq improved metabolism of adipocytes by enhancing genes associated with energy homeostasis and insulin sensitivity, SIRT1, PRKAA2, and IRS1. Interestingly, Sq increased significantly early adipogenic markers and lipogenic gene expression such as PPARG, SREBF1, and CEBPA, but not HH-Sq. As a consequence, smaller and fewer lipid droplet formation was observed in HH-Sq-treated adipocytes. Based on our findings, we report that both Sq and HH-Sq improved adipocyte metabolism, but only HH-Sq prevented excessive lipogenesis without abrogating adipocyte differentiation. The beneficial effect of HH-Sq provides an importance of synthesized derivatives from a natural compound with therapeutic potentials in the application of cell therapies.
Munkhzul Ganbold; Farhana Ferdousi; Takashi Arimura; Kenichi Tominaga; Hiroko Isoda. New Amphiphilic Squalene Derivative Improves Metabolism of Adipocytes Differentiated From Diabetic Adipose-Derived Stem Cells and Prevents Excessive Lipogenesis. Frontiers in Cell and Developmental Biology 2020, 8, 1 .
AMA StyleMunkhzul Ganbold, Farhana Ferdousi, Takashi Arimura, Kenichi Tominaga, Hiroko Isoda. New Amphiphilic Squalene Derivative Improves Metabolism of Adipocytes Differentiated From Diabetic Adipose-Derived Stem Cells and Prevents Excessive Lipogenesis. Frontiers in Cell and Developmental Biology. 2020; 8 ():1.
Chicago/Turabian StyleMunkhzul Ganbold; Farhana Ferdousi; Takashi Arimura; Kenichi Tominaga; Hiroko Isoda. 2020. "New Amphiphilic Squalene Derivative Improves Metabolism of Adipocytes Differentiated From Diabetic Adipose-Derived Stem Cells and Prevents Excessive Lipogenesis." Frontiers in Cell and Developmental Biology 8, no. : 1.
Prohydrojasmon (PDJ) can improve the polyphenol and anthocyanin content and antioxidant activity of some crop plants, but it also shows a suppressive effect on the plant growth. This study aimed to investigate the inhibitory effect of PDJ on the growth of two crop plants: komatsuna (Brassica rapa var. periviridis) and eggplant (Solanum melongena). We applied various concentrations of PDJ drip-wise or by spraying to eggplant and komatsuna seedlings and made detailed observations of growth. In general, no significant suppressive effect of PDJ was observed in the aerial parts in both plants. However, a significant inhibitory effect was found in roots treated with PDJ at concentrations of 600 and 1000 ppm. Interestingly, komatsuna treated with PDJ at a concentration of 200 ppm in both approaches resulted in a significant increase in root weight up to 37%. At a concentration range of 200–400 ppm, PDJ showed no inhibitory effects, and in some cases slightly promoted root growth. Therefore, this could be the recommended concentration range. We conclude that application of PDJ can still be beneficial to the vegetable crops without causing serious inhibition or suppression effects on the growth, as long as it is kept at rather low concentrations.
Haidar Rafid Azis; Shinya Takahashi; Masami Koshiyama; Hiroshi Fujisawa; Hiroko Isoda. Effect of Prohydrojasmon on the Growth of Eggplant and Komatsuna. Plants 2020, 9, 1368 .
AMA StyleHaidar Rafid Azis, Shinya Takahashi, Masami Koshiyama, Hiroshi Fujisawa, Hiroko Isoda. Effect of Prohydrojasmon on the Growth of Eggplant and Komatsuna. Plants. 2020; 9 (10):1368.
Chicago/Turabian StyleHaidar Rafid Azis; Shinya Takahashi; Masami Koshiyama; Hiroshi Fujisawa; Hiroko Isoda. 2020. "Effect of Prohydrojasmon on the Growth of Eggplant and Komatsuna." Plants 9, no. 10: 1368.