The concept of allogenic tooth transplantation has been known since the early stages of modern oral surgery. Despite numerous successful clinical cases, a deeper understanding of the biological and technical aspects of this method is still required to enable its safe and effective implementation in routine clinical practice. In this case report, we describe an allogenic tooth transplantation performed between a mother and her son. In the mother, teeth 14 and 24 had been extracted more than 10 years earlier due to failed endodontic treatment. Only minimal spontaneous space closure between adjacent teeth occurred, and therefore orthodontic space reopening was not required prior to transplantation. The donor teeth were the son’s maxillary first premolars, which were indicated for extraction for orthodontic reasons. The procedure was preceded by a detailed CBCT analysis, establishment of a surgical plan, and definition of the transplantation protocol. Using CAD/CAM-based 3D printing, replicas of the donor teeth were fabricated and used intraoperatively as guides during preparation of the recipient sites within the alveolar bone. The recipient sockets were prepared using instruments from an implant surgical kit. The 3D tooth models enabled precise adaptation of the recipient bed to the morphology of the donor roots, thereby minimizing the extra-alveolar time. Following atraumatic extraction, the donor teeth were immediately transplanted into the recipient. This report presents a case combining 3D printing technology with allogenic tooth transplantation. This approach allows reduction of extra-alveolar time, minimizes unnecessary alveolar bone loss, and improves primary stability of the transplant. The aim of this study is to expand current knowledge regarding the potential use of allogenic tooth transplantation in combination with 3D printing and to support its broader application in clinical dental practice, similarly to other medical fields where transplantation procedures demonstrate high success rates.