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Prof. M.Y. Rafii
Universiti Putra Malaysia

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0 Plant Breeding
0 Plant biodiversity
0 Plant Sciences
0 Breeding and genetics
0 Molecular breeding,

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Plant Breeding
Molecular breeding,
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Short Biography

Prof Rafii Bin Yusop Has published 383 Research Items, 1545928 Reads and 5196 Citations, and Skills and Expertise is Genetics Marker Assisted Selection Plant Breeding Molecular Plant Breeding

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Review
Published: 20 August 2021 in Plants
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Eggplant is the fifth economically most important vegetable in the Solanaceae family after tomato, potato, chili, and tobacco. Apart from the well-cultivated brinjal or aubergine eggplant (Solanum melongena L.), two other underutilized eggplant species, the African eggplant (S. macrocarpon L.) and the scarlet eggplant (S. aethiopicum L.), were also cultivated with local importance where the leaves and fruits are used for food and medicinal purposes. The major objectives of the eggplant breeding program are to improve fruit quality, increase yield performance through heterosis breeding, and introduce pest and disease resistances from wild relatives. Europe and Asia hold a wide collection of germplasm resources with significant potential for genetic improvement. While cultivated eggplant is susceptible to several fungi and bacteria, many wild relatives offer potential resistance to these pathogens. In this paper, we review the genetic resources and diversity of cultivated eggplant and its wild relatives. As a point of departure, we examine the economic importance, domestication, taxonomy characterization, and relationships of the crop and its wild relatives. The importance of evaluating and safeguarding wild relatives is highlighted, as crop wild relatives are highly underrepresented. A key section in this study is an overview dedicated to genetic resources, resistance to biotic and abiotic stresses, pre-breeding, and breeding for sustainable eggplant production.

ACS Style

Yusuff Oladosu; Mohd Y. Rafii; Fatai Arolu; Samuel Chibuike Chukwu; Monsuru Adekunle Salisu; Bolanle Amudalat Olaniyan; Ifeoluwa Kayode Fagbohun; Taoheed Kolawole Muftaudeen. Genetic Diversity and Utilization of Cultivated Eggplant Germplasm in Varietal Improvement. Plants 2021, 10, 1714 .

AMA Style

Yusuff Oladosu, Mohd Y. Rafii, Fatai Arolu, Samuel Chibuike Chukwu, Monsuru Adekunle Salisu, Bolanle Amudalat Olaniyan, Ifeoluwa Kayode Fagbohun, Taoheed Kolawole Muftaudeen. Genetic Diversity and Utilization of Cultivated Eggplant Germplasm in Varietal Improvement. Plants. 2021; 10 (8):1714.

Chicago/Turabian Style

Yusuff Oladosu; Mohd Y. Rafii; Fatai Arolu; Samuel Chibuike Chukwu; Monsuru Adekunle Salisu; Bolanle Amudalat Olaniyan; Ifeoluwa Kayode Fagbohun; Taoheed Kolawole Muftaudeen. 2021. "Genetic Diversity and Utilization of Cultivated Eggplant Germplasm in Varietal Improvement." Plants 10, no. 8: 1714.

Journal article
Published: 17 August 2021 in Agronomy
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The knowledge of genetic variability and breeding techniques is crucial in crop improvement programs. This information is especially important in underutilized crops such as Bambara groundnut, which have limited breeding systems and genetic diversity information. Hence, this study evaluated the genetic variability and established the relationship between the yield and its components in Bambara groundnut based on seed weight using multivariate analysis. A field trial was conducted in a randomized complete block design with three replications on 28 lines. Data were collected on 12 agro-morphological traits, and a statistical analysis was conducted using SAS version 9.4 software, while the variance component, genotypic and phenotypic coefficient variation, heritability, and genetic advance values were estimated. A cluster analysis was performed using NT-SYS software to estimate the genetic relations among the accessions. The results showed significant variability among the accessions based on the yield and yield component characteristics. The evaluated lines were grouped into seven primary clusters based on the assessed traits using the UPGMA dendrogram. Based on the overall results, G5LR1P3, G1LR1P3, G4LR1P1, G2SR1P1 and G3SR1P4 performed the best for the yield and yield components. These improved lines are recommended for large-scale evaluation and utilization in future breeding programs to develop high-yield Bambara groundnut varieties.

ACS Style

Atiqullah Khaliqi; Mohd Y. Rafii; Norida Mazlan; Mashitah Jusoh; Yusuff Oladosu. Genetic Analysis and Selection Criteria in Bambara Groundnut Accessions Based Yield Performance. Agronomy 2021, 11, 1634 .

AMA Style

Atiqullah Khaliqi, Mohd Y. Rafii, Norida Mazlan, Mashitah Jusoh, Yusuff Oladosu. Genetic Analysis and Selection Criteria in Bambara Groundnut Accessions Based Yield Performance. Agronomy. 2021; 11 (8):1634.

Chicago/Turabian Style

Atiqullah Khaliqi; Mohd Y. Rafii; Norida Mazlan; Mashitah Jusoh; Yusuff Oladosu. 2021. "Genetic Analysis and Selection Criteria in Bambara Groundnut Accessions Based Yield Performance." Agronomy 11, no. 8: 1634.

Review
Published: 17 August 2021 in Agronomy
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Rice, generally classified as a typical glycophyte, often faces abiotic stresses such as excessive drought, high salinity, prolonged submergence, cold, and temperature, which significantly affects growth, development, and ultimately, grain yield. Among these negative impacts of abiotic factors in rice production, salinity stress is a major constraint, followed by drought. There is considerable research on the use of marker-assisted selection (MAS), genome editing techniques, and transgenic studies that have profoundly improved the present-day rice breeders’ toolboxes for developing salt-tolerant varieties. Salinity stresses significantly affect rice plants during seedling and reproductive stages. Hence, greater understanding and manipulation of genetic architecture in developing salt-tolerant rice varieties will significantly impact sustainable rice production. Rice plants’ susceptibility or tolerance to high salinity has been reported to be the result of coordinated actions of multiple stress-responsive quantitative trait loci (QTLs)/genes. This paper reviews recent literature, updating the effects of salinity stress on rice plants and germplasm collections and screening for salinity tolerance by different breeding techniques. Mapping and identification of QTLs salt tolerance genes are illuminated. The present review updates recent breeding for improvement in rice tolerance to salinity stress and how state-of-the-art tools such as MAS or genetic engineering and genome editing techniques, including mutagenesis and conventional breeding techniques, can assist in transferring salt-tolerant QTLs genes into elite rice genotypes, accelerating breeding of salt-resistant rice cultivars.

ACS Style

Azadul Haque; Mohd Y. Rafii; Martini Mohammad Yusoff; Nusaibah Syd Ali; Oladosu Yusuff; Debi Rani Datta; Mohammad Anisuzzaman; Mohammad Ferdous Ikbal. Advanced Breeding Strategies and Future Perspectives of Salinity Tolerance in Rice. Agronomy 2021, 11, 1631 .

AMA Style

Azadul Haque, Mohd Y. Rafii, Martini Mohammad Yusoff, Nusaibah Syd Ali, Oladosu Yusuff, Debi Rani Datta, Mohammad Anisuzzaman, Mohammad Ferdous Ikbal. Advanced Breeding Strategies and Future Perspectives of Salinity Tolerance in Rice. Agronomy. 2021; 11 (8):1631.

Chicago/Turabian Style

Azadul Haque; Mohd Y. Rafii; Martini Mohammad Yusoff; Nusaibah Syd Ali; Oladosu Yusuff; Debi Rani Datta; Mohammad Anisuzzaman; Mohammad Ferdous Ikbal. 2021. "Advanced Breeding Strategies and Future Perspectives of Salinity Tolerance in Rice." Agronomy 11, no. 8: 1631.

Article
Published: 15 July 2021 in Scientific Reports
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As a new crop in Malaysia, forty-four Bambara groundnut (Vigna subterranea L. verdc.) genotypes were sampled from eleven distinct populations of different origins to explore the genetic structure, genetic inconsistency, and fixation index. The Bambara groundnut, an African underutilized legume, has the capacity to boost food and nutrition security while simultaneously addressing environmental sustainability, food availability, and economic inequalities. A set of 32 ISSRs were screened out of 96 primers based on very sharp, clear, and reproducible bands which detected a total of 510 loci with an average of 97.64% polymorphism. The average calculated value of PIC = 0.243, RP = 5.30, H = 0.285, and MI = 0.675 representing the efficiency of primer set for genetic differentiation among the genotypes. The ISSR primers revealed the number of alleles (Na = 1.97), the effective number of alleles (Ne = 1.38), Nei's genetic diversity (h = 0.248), and a moderate level of gene flow (Nm = 2.26) across the genotypes studied. The estimated Shannon’s information index (I = 0.395) indicates a high level of genetic variation exists among the accessions. Based on Nei’s genetic dissimilarity a UPMGA phylogenetic tree was constructed and grouped the entire genotypes into 3 major clusters and 6 subclusters. PCA analysis revealed that first principal component extracted maximum variation (PC1 = 13.92%) than second principal component (PC2 = 12.59%). Bayesian model-based STRUCTURE analysis assembled the genotypes into 3 (best ΔK = 3) genetic groups. The fixation-index (Fst) analysis narrated a very great genetic diversity (Fst = 0.19 to 0.40) exists within the accessions of these 3 clusters. This investigation specifies the effectiveness of the ISSR primers system for the molecular portrayal of V. subterranea genotypes that could be used for genetic diversity valuation, detection, and tagging of potential genotypes with quick, precise, and authentic measures for this crop improvement through effective breeding schemes.

ACS Style

Mahmudul Hasan Khan; Mohd Y. Rafii; Shairul Izan Ramlee; Mashitah Jusoh; Al Mamun; Jamilu Halidu. DNA fingerprinting, fixation-index (Fst), and admixture mapping of selected Bambara groundnut (Vigna subterranea [L.] Verdc.) accessions using ISSR markers system. Scientific Reports 2021, 11, 1 -23.

AMA Style

Mahmudul Hasan Khan, Mohd Y. Rafii, Shairul Izan Ramlee, Mashitah Jusoh, Al Mamun, Jamilu Halidu. DNA fingerprinting, fixation-index (Fst), and admixture mapping of selected Bambara groundnut (Vigna subterranea [L.] Verdc.) accessions using ISSR markers system. Scientific Reports. 2021; 11 (1):1-23.

Chicago/Turabian Style

Mahmudul Hasan Khan; Mohd Y. Rafii; Shairul Izan Ramlee; Mashitah Jusoh; Al Mamun; Jamilu Halidu. 2021. "DNA fingerprinting, fixation-index (Fst), and admixture mapping of selected Bambara groundnut (Vigna subterranea [L.] Verdc.) accessions using ISSR markers system." Scientific Reports 11, no. 1: 1-23.

Journal article
Published: 07 July 2021 in Agronomy
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This study investigated oil palm inflorescence sex ratio (ISR) and fruit set (FS) yield among 24 dura × pisifera biparental progenies on fibric peat soil, hybridized from ten genetic origins. Three-month intervals of data collection on ISR and “S-shape” procedure for FS determination were carried out in an independent complete randomized design for one year. Based on the assessment of individual progenies, analysis of variance revealed the existence of genetic heterogeneity. Five of the progenies had the highest ISR above 90%. However, 20.83% of the progenies (PK4841, PK4674, ECPHP415, ECPHP550, and PK4118) had the best normal range of ISR, indicating FS declined being influenced by ISR. Therefore, only 12.50% of the progenies (PK4674, PK4465, and PK4482) were above 60% FS critical level. Correlation analysis verified that ISR depends on the female flower and progeny PK4674 (65.78%) and PK4465 (62.62%) had the best FS and fruit to bunch. Among the parental origins, Deli Serdang × Cameroon was statistically the best for male flower production at 29.40% and ISR at 72.22%. The highest FS was recorded in Deli Banting × AVROS (54.00%) and Angola × AVROS (53.78%). However, molecular research, such as microsatellite molecular markers if concurrently used as a selection criterion with the present conventional study, could present further details to complement the present findings.

ACS Style

Senesie Swaray; Mohd Amiruddin; Mohd Rafii; Syari Jamian; Mohd Ismail; Momodu Jalloh; Mazli Eswa; Marhalil Marjuni; Ibrahim Akos; Oladosu Yusuff. Oil Palm Inflorescence Sex Ratio and Fruit Set Assessment in dura × pisifera Biparental Progenies on Fibric Peat Soil. Agronomy 2021, 11, 1380 .

AMA Style

Senesie Swaray, Mohd Amiruddin, Mohd Rafii, Syari Jamian, Mohd Ismail, Momodu Jalloh, Mazli Eswa, Marhalil Marjuni, Ibrahim Akos, Oladosu Yusuff. Oil Palm Inflorescence Sex Ratio and Fruit Set Assessment in dura × pisifera Biparental Progenies on Fibric Peat Soil. Agronomy. 2021; 11 (7):1380.

Chicago/Turabian Style

Senesie Swaray; Mohd Amiruddin; Mohd Rafii; Syari Jamian; Mohd Ismail; Momodu Jalloh; Mazli Eswa; Marhalil Marjuni; Ibrahim Akos; Oladosu Yusuff. 2021. "Oil Palm Inflorescence Sex Ratio and Fruit Set Assessment in dura × pisifera Biparental Progenies on Fibric Peat Soil." Agronomy 11, no. 7: 1380.

Journal article
Published: 21 May 2021 in BioMed Research International
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High kernel elongation (HKE) is one of the high-quality characteristics in rice. The objectives of this study were to determine the effects of ageing treatments, gene actions, and inheritance pattern of kernel elongation on cooking quality in two populations of rice and determine the path of influence and contribution of other traits to kernel elongation in rice. Two rice populations derived from crosses between MR219 × Mahsuri Mutan and MR219 × Basmati 370 were used. The breeding materials included two F1 progenies from the two populations, and their respective parents were grown in four different batches at a week interval to synchronize the flowering between the female and male plants. Scaling tests and generation means analysis were carried out to determine ageing effects and estimate additive-dominance gene action and epistasis. The estimation of gene interaction was based on quantitative traits. Path coefficient analysis was done using SAS software version 9.4 to determine the path of influence (direct or indirect) of six quantitative traits on HKE. Results obtained showed that nonallelic gene interaction was observed in all traits. The results before ageing and after ageing showed significant differences in all traits, while the gene interaction changed after ageing. The HKE value improved after ageing, suggesting that ageing is an external factor that could influence gene expression. The epistasis effect for HKE obtained from the cross Mahsuri Mutan × MR219 showed duplicate epistasis while that obtained from a cross between Basmati 370 × MR219 showed complimentary epistasis. Besides, the heritability of HKE was higher in Basmati 370 × MR219 compared to that obtained in Mahsuri Mutan × MR219. The path analysis showed that the cooked grain length and length-width ratio positively significantly affected HKE. It was concluded that ageing treatment is an external factor that could improve the expression of HKE. The findings from this study would be useful to breeders in the selection and development of new specialty (HKE) rice varieties.

ACS Style

Anna Arina Bt Ab. Halim; Mohd Y. Rafii; Mohamad B. Osman; Yusuff Oladosu; Samuel C. Chukwu. Ageing Effects, Generation Means, and Path Coefficient Analyses on High Kernel Elongation in Mahsuri Mutan and Basmati 370 Rice Populations. BioMed Research International 2021, 2021, 1 -20.

AMA Style

Anna Arina Bt Ab. Halim, Mohd Y. Rafii, Mohamad B. Osman, Yusuff Oladosu, Samuel C. Chukwu. Ageing Effects, Generation Means, and Path Coefficient Analyses on High Kernel Elongation in Mahsuri Mutan and Basmati 370 Rice Populations. BioMed Research International. 2021; 2021 ():1-20.

Chicago/Turabian Style

Anna Arina Bt Ab. Halim; Mohd Y. Rafii; Mohamad B. Osman; Yusuff Oladosu; Samuel C. Chukwu. 2021. "Ageing Effects, Generation Means, and Path Coefficient Analyses on High Kernel Elongation in Mahsuri Mutan and Basmati 370 Rice Populations." BioMed Research International 2021, no. : 1-20.

Review
Published: 15 May 2021 in Sustainability
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The world’s food and agricultural schemes have gradually fallen into an alarming state due to challenges such as high population birth rates, diverse agro-climatic zones, a lack of measures to counter global warming, severe practices of sole-culture cultivation, and asset reduction. A very high dependency on limited staple food crops is associated with repetitious diets, deprivation of food, and shortages of trace minerals, which often causes dietary sicknesses. To ensure nutritious diets worldwide, a real-world and justifiable scheme is provided to garner extra attention towards variation in both agriculture/farming approaches and food habits. The EAT-Lancet statement emphasized an increase in agri-based diets as a way of attaining global generational health. Enlarging neglected crops with plenty of genomic stocks and potentially profitable attributes is a solution that could address food and nutritional security concerns. Bambara groundnut is one such imperative and neglected legume crop that contributes positively to improving global food and nutrient safety. As a “complete food”, this crop has recently been treated as a new millennium crop, and furthermore, it is more adjusted to poor soil and climatic conditions than other dominant crops. Bambara groundnut is a repository of vital nutrients that provides carbohydrates, crucial amino acids, proteins, and energy as well as minerals and vitamins to developed and low-income countries where animal proteins are not readily available. This review explores the potential of Bambara groundnut in ensuring food and nutrient security; its variables, production, processing, nutrient values, role in reducing the nutritional gap, and diverse uses; and attempts in improving its traits. To strengthen food production, an agricultural revolution is required for underutilized crop species to feed the ever-expanding population in the world. Henceforth, advanced plant-breeding procedures, such as next-generation breeding techniques, various molecular tools, TILLING, Eco-TILLING, proteomics, genomics, and transcriptomics (which has been used for major crops), also need to be practiced to intensify production. To boost productivity and to feed the most starved and malnourished populations of the world, it is assumed that the application of modern techniques will play a vital role in the advancement of the underutilized Bambara groundnut.

ACS Style

Mahmudul Hasan Khan; Mohd Rafii; Shairul Ramlee; Mashitah Jusoh; Al- Mamun. Bambara Groundnut (Vigna subterranea L. Verdc): A Crop for the New Millennium, Its Genetic Diversity, and Improvements to Mitigate Future Food and Nutritional Challenges. Sustainability 2021, 13, 5530 .

AMA Style

Mahmudul Hasan Khan, Mohd Rafii, Shairul Ramlee, Mashitah Jusoh, Al- Mamun. Bambara Groundnut (Vigna subterranea L. Verdc): A Crop for the New Millennium, Its Genetic Diversity, and Improvements to Mitigate Future Food and Nutritional Challenges. Sustainability. 2021; 13 (10):5530.

Chicago/Turabian Style

Mahmudul Hasan Khan; Mohd Rafii; Shairul Ramlee; Mashitah Jusoh; Al- Mamun. 2021. "Bambara Groundnut (Vigna subterranea L. Verdc): A Crop for the New Millennium, Its Genetic Diversity, and Improvements to Mitigate Future Food and Nutritional Challenges." Sustainability 13, no. 10: 5530.

Journal article
Published: 10 May 2021 in Rice Science
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Improved rice lines were developed frome three parents with the resistance or tolerance to bacterial leaf blight, blast and drought stress, respectively, using single-, double- and three-way crosses. The improved lines were assessed for agro-morphological and yield traits under non-drought stress (NS) and reproductive-stage drought stress (RS) treatments. The mean comparison of traits measured between parent plants and progenies (improved lines) were similar, and there were significant and non-significant differences among the parents and improved lines (genotypes) under NS and RS. Smilarly, there was significant and non-significant differences in the interaction among both parent varieties and improved lines for NS and RS. Cluster and 3D-model of principal component analysis did not generate categorical clusters according to crossing methods, and there were no exclusive crossing method inclined variations under the treatments. The improved lines were high-yielding, disease resistant, and drought-tolerant compared with their parents. All the crossing methods were good for this crop improvement program without preference to any, despite the number of genes introgressed.

ACS Style

Ibrahim Silas Akos; Mohd Y. Rafii; Mohd Razi Ismail; Shairul Izan Ramlee; Noraziyah Abd Aziz Shamsudin; Asfaliza Ramli; Samuel Chibuike Chukwu; Senesie Swaray; Momodu Jalloh. Evaluation of Inherited Resistance Genes of Bacterial Leaf Blight, Blast and Drought Tolerance in Improved Rice Lines. Rice Science 2021, 28, 279 -288.

AMA Style

Ibrahim Silas Akos, Mohd Y. Rafii, Mohd Razi Ismail, Shairul Izan Ramlee, Noraziyah Abd Aziz Shamsudin, Asfaliza Ramli, Samuel Chibuike Chukwu, Senesie Swaray, Momodu Jalloh. Evaluation of Inherited Resistance Genes of Bacterial Leaf Blight, Blast and Drought Tolerance in Improved Rice Lines. Rice Science. 2021; 28 (3):279-288.

Chicago/Turabian Style

Ibrahim Silas Akos; Mohd Y. Rafii; Mohd Razi Ismail; Shairul Izan Ramlee; Noraziyah Abd Aziz Shamsudin; Asfaliza Ramli; Samuel Chibuike Chukwu; Senesie Swaray; Momodu Jalloh. 2021. "Evaluation of Inherited Resistance Genes of Bacterial Leaf Blight, Blast and Drought Tolerance in Improved Rice Lines." Rice Science 28, no. 3: 279-288.

Research article
Published: 04 May 2021 in BioMed Research International
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Molecular characterization of oil palm germplasm is crucial in utilizing and conserving germplasm with promising traits. This study was conducted to evaluate the genetic diversity structures and relationships among 26 families of MPOB-Senegal oil palm germplasm using thirty-five microsatellite markers. High level of polymorphism ( P = 96.26 %), number of effective allele ( N e = 2.653 ), observed heterozygosity ( H o = 0.584 ), expected heterozygosity ( H e = 0.550 ), total heterozygosity ( H T = 0.666 ), and rare alleles (54) were observed which indicates that MPOB-Senegal germplasm has a broad genetic variation. Among the SSR markers, sMo00053 and sMg00133 were the most informative markers for discrimination among the MPOB-Senegal oil palm germplasm for having the highest private alleles and the rare alleles. For selection and conservation, oil palm populations with high rare alleles and Nei’s gene diversity index should be considered as these populations may possess unique genes for further exploitation.

ACS Style

Khin Aye Myint; Zulkifli Yaakub; Mohd Y. Rafii; Yusuff Oladosu; Mohd Yusoff Abd Samad; Shairul Izan Ramlee; Suzana Mustaffa; Fatai Arolu; Norziha Abdullah; Marhalil Marjuni; Mohd Din Amiruddin. Genetic Diversity Assessment of MPOB-Senegal Oil Palm Germplasm Using Microsatellite Markers. BioMed Research International 2021, 2021, 1 -14.

AMA Style

Khin Aye Myint, Zulkifli Yaakub, Mohd Y. Rafii, Yusuff Oladosu, Mohd Yusoff Abd Samad, Shairul Izan Ramlee, Suzana Mustaffa, Fatai Arolu, Norziha Abdullah, Marhalil Marjuni, Mohd Din Amiruddin. Genetic Diversity Assessment of MPOB-Senegal Oil Palm Germplasm Using Microsatellite Markers. BioMed Research International. 2021; 2021 ():1-14.

Chicago/Turabian Style

Khin Aye Myint; Zulkifli Yaakub; Mohd Y. Rafii; Yusuff Oladosu; Mohd Yusoff Abd Samad; Shairul Izan Ramlee; Suzana Mustaffa; Fatai Arolu; Norziha Abdullah; Marhalil Marjuni; Mohd Din Amiruddin. 2021. "Genetic Diversity Assessment of MPOB-Senegal Oil Palm Germplasm Using Microsatellite Markers." BioMed Research International 2021, no. : 1-14.

Journal article
Published: 20 April 2021 in Sustainability
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Specialty fragrant rice is sold at a premium price in both local and international trade because of its superior grain qualities. In this research, 40 advanced fragrant rice accessions were evaluated in different environments. The primary objective was to identify genotypes with high grain yield and high stability using multivariate (GGE biplot) and univariate analysis (regression slope, deviation from regression, Shukla’s stability variance, Wricke’s ecovalence, and Kang’s stability statistic). The field experiment trials were laid in a randomized complete block design in three replications. The analysis of variance showed highly significant differences among genotypes, locations, seasons, and the interactions between genotype, locations, and seasons. The environment significantly explained about 43.32% (37.01 and 6.31% for locations and seasons) of the total sum of squares. Based on average ranking generated from multivariate and univariate stability measured, rice accessions were classified into three major categories, viz., genotypes having high trait performance, and high stability as category 1. The second category consists of genotypes that exhibit high mean performance but low stability, while the third category includes genotypes with high stability but low trait performance. Our results showed that breeding for yield performance was possible, and the identified genotypes could be recommended for commercial cultivation.

ACS Style

Norainy Hashim; Mohd Rafii; Yusuff Oladosu; Mohd Ismail; Asfaliza Ramli; Fatai Arolu; Samuel Chukwu. Integrating Multivariate and Univariate Statistical Models to Investigate Genotype–Environment Interaction of Advanced Fragrant Rice Genotypes under Rainfed Condition. Sustainability 2021, 13, 4555 .

AMA Style

Norainy Hashim, Mohd Rafii, Yusuff Oladosu, Mohd Ismail, Asfaliza Ramli, Fatai Arolu, Samuel Chukwu. Integrating Multivariate and Univariate Statistical Models to Investigate Genotype–Environment Interaction of Advanced Fragrant Rice Genotypes under Rainfed Condition. Sustainability. 2021; 13 (8):4555.

Chicago/Turabian Style

Norainy Hashim; Mohd Rafii; Yusuff Oladosu; Mohd Ismail; Asfaliza Ramli; Fatai Arolu; Samuel Chukwu. 2021. "Integrating Multivariate and Univariate Statistical Models to Investigate Genotype–Environment Interaction of Advanced Fragrant Rice Genotypes under Rainfed Condition." Sustainability 13, no. 8: 4555.

Journal article
Published: 07 April 2021 in Scientific Reports
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As a crop for the new millennium Bambara groundnut (Vigna subterranea [L.] Verdc.) considered as leading legumes in the tropical regions due to its versatile advantages. The main intent of this study was to find out the high yielding potential genotypes and considering these genotypes to develop pure lines for commercial cultivation in Malaysia. Considering the 14 qualitative and 27 quantitative traits of fifteen landraces the variation and genetic parameters namely, variability, heritability, genetic advance, characters association, and cluster matrix were determined. ANOVA revealed significant variation for all the agronomic traits (except plant height). Among the accessions, highly significant differences (P ≤ 0.01) were found for almost all the traits excluding fifty percent flowering date, seed length, seed width. The 16 traits out of the 27 quantitative traits had a coefficient of variation (CV) ≥ 20%. A positive and intermediate to perfect highly significant association (r = 0.23 to 1.00; P < 0.00) was found between yield and its related traits. The trait dry seed weight per plant (g) had the highest GCV = 59.91% and PCV = 59.57% whereas the trait fresh pod weight (99.55%), dry seed weight (98.86%), and yield (98.10%) were highly heritable. The genetic advance recorded the highest for dry seed weight (122.01%) and lowest (3.97%) for plant height. To validate the genetic disparity, an unweighted pair-group produce with arithmetic mean (UPGMA), principal component analysis (PCA), heatmap, and H’-index was performed considering 27 quantitative traits. The constructed dendrogram showed five distinct groups of accessions. Genotypes G2, G3, and G9 from Group IV consider as promising lines which gave 70.05% higher mean yield compared to grand mean yield (1180 kg ha−1) with desirable traits. Group II had a maximum number of accessions while group III and group V had one of each. However, findings declared that the availability of genetic variance will be beneficial for this crop improvement through cross breeding as well as the plant breeders to prefer desirable traits in V. subterranea L. Verdc. for further breeding purposes.

ACS Style

Mahmudul Hasan Khan; Mohd Y. Rafii; Shairul Izan Ramlee; Mashitah Jusoh; Al Mamun. Genetic analysis and selection of Bambara groundnut (Vigna subterranea [L.] Verdc.) landraces for high yield revealed by qualitative and quantitative traits. Scientific Reports 2021, 11, 1 -21.

AMA Style

Mahmudul Hasan Khan, Mohd Y. Rafii, Shairul Izan Ramlee, Mashitah Jusoh, Al Mamun. Genetic analysis and selection of Bambara groundnut (Vigna subterranea [L.] Verdc.) landraces for high yield revealed by qualitative and quantitative traits. Scientific Reports. 2021; 11 (1):1-21.

Chicago/Turabian Style

Mahmudul Hasan Khan; Mohd Y. Rafii; Shairul Izan Ramlee; Mashitah Jusoh; Al Mamun. 2021. "Genetic analysis and selection of Bambara groundnut (Vigna subterranea [L.] Verdc.) landraces for high yield revealed by qualitative and quantitative traits." Scientific Reports 11, no. 1: 1-21.

Journal article
Published: 04 March 2021 in Insects
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This study was conducted to assess the Elaeidobius kamerunicus (EK) population density among the biparental dura × pisifera hybrids’ palms on deep peat-soil. Twenty-four hybrids derived from 10 genetic sources were used. Variance analysis showed that the EK population density varies between different oil palm hybrids, with a more noticeable variation of a low population mean in the male weevil across the hybrids. The highest weevil population mean/spikelet was attained on the third day of anthesis. The maximum monthly population of EK/spikelet (12.81 ± 0.23) and population density of EK (1846.49 ± 60.69) were recorded in January. Accordingly, 41.67% of the hybrids recorded an EK population density greater than the trial means of 973.68 weevils. Hybrid ECPHP550 had the highest mean of EK/spikelet (10.25 ± 0.11) and the highest population density of EK/palm (1241.39 ± 73.74). The parental mean population was 963.24 weevils and parent Deli-Banting × AVROS recorded the highest EK population density (1173.01). The overall results showed a notable disparity in the EK population among the biparental hybrids. Parental Deli-Banting × AVROS and hybrid ECPHP550 could be more useful to optimize the weevil population for pollination improvements in palm plantations. However, we suggest that volatile production should be included as a desirable trait in oil palm selective breeding.

ACS Style

Senesie Swaray; Mohd Y. Rafii; Mohd Din Amiruddin; Mohd Firdaus Ismail; Syari Jamian; Momodu Jalloh; Yusuff Oladosu; Mohd Mustakim Mohamad; Marhalil Marjuni; Olalekan Kolapo; Samuel Chukwu. Assessment of Oil Palm Pollinating Weevil (Elaeidobius kamerunicus) Population Density in Biparental dura × pisifera Hybrids on Deep Peat-Soil in Perak State, Malaysia. Insects 2021, 12, 221 .

AMA Style

Senesie Swaray, Mohd Y. Rafii, Mohd Din Amiruddin, Mohd Firdaus Ismail, Syari Jamian, Momodu Jalloh, Yusuff Oladosu, Mohd Mustakim Mohamad, Marhalil Marjuni, Olalekan Kolapo, Samuel Chukwu. Assessment of Oil Palm Pollinating Weevil (Elaeidobius kamerunicus) Population Density in Biparental dura × pisifera Hybrids on Deep Peat-Soil in Perak State, Malaysia. Insects. 2021; 12 (3):221.

Chicago/Turabian Style

Senesie Swaray; Mohd Y. Rafii; Mohd Din Amiruddin; Mohd Firdaus Ismail; Syari Jamian; Momodu Jalloh; Yusuff Oladosu; Mohd Mustakim Mohamad; Marhalil Marjuni; Olalekan Kolapo; Samuel Chukwu. 2021. "Assessment of Oil Palm Pollinating Weevil (Elaeidobius kamerunicus) Population Density in Biparental dura × pisifera Hybrids on Deep Peat-Soil in Perak State, Malaysia." Insects 12, no. 3: 221.

Journal article
Published: 01 January 2021 in Journal of Radiation Research and Applied Sciences
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Bambara groundnut is a highly nutritious underutilized legume with enormous potential to sustain food security in resource-poor countries. However, its potential for improvement through conventional breeding (< 2% success rate) limitation due to the nature of the flowers. Thus, the most viable method of improving this crop is by creating genetic variability through induced mutagenesis. The present study was conducted to evaluate the radiosensitivity of two Bambara groundnut varieties irradiated with acute and chronic gamma irradiations to determine the lethal dose (LD) and growth reduction dose (GR). Healthy seeds of both varieties were exposed to acute gamma irradiation using Cesium-137 at 0, 25, 50, 75, 100, 125, 150, 175, 200, 250, and 300 Gy. For chronic irradiation, two-week-old seedlings of the two genotypes were exposed to accumulated doses of 0, 8.52, 17.04, 35.56, 34.09, 42.61, 59.65, 93.74, 144.87, 255.64, and 570.94 Gy, respectively, in Gamma Green House (GGH) for 60 days. The result from the variance analysis indicated highly significant differences (P < 0.01) for all evaluated traits except for internode length. A linear regression model was developed to determine the mean LD and GR of both genotypes. The established lethal doses (LD25, 50, 75) for acute gamma irradiation on Ex-Sokoto variety were 75, 160, and 250 Gy while 68, 148, and 227 Gy were recorded for Karo variety, respectively. For chronic irradiation, the established growth reduction doses for Ex-Sokoto were 47, 250, and 444 Gy, whereas 70, 264, and 452 Gy were observed in Karo. Variations were observed between the gamma-irradiated genotypes and the methods of irradiations. Generally, the growth, development, and survival rate of Bambara groundnut increase with a decrease in gamma-irradiation doses. The established LD and GR doses from this study can be utilized in large-scale mutagenesis breeding programs for generating a wide range of mutants in Bambara groundnut.

ACS Style

Ismaila Muhammad; Mohd Y. Rafii; Muhamad Hazim Nazli; Shairul Izan Ramlee; Abdul Rahim Harun; Yusuff Oladosu. Determination of lethal (LD) and growth reduction (GR)doses on acute and chronic gamma- irradiated Bambara groundnut [Vigna subterranea (L.) Verdc.]varieties. Journal of Radiation Research and Applied Sciences 2021, 14, 133 -145.

AMA Style

Ismaila Muhammad, Mohd Y. Rafii, Muhamad Hazim Nazli, Shairul Izan Ramlee, Abdul Rahim Harun, Yusuff Oladosu. Determination of lethal (LD) and growth reduction (GR)doses on acute and chronic gamma- irradiated Bambara groundnut [Vigna subterranea (L.) Verdc.]varieties. Journal of Radiation Research and Applied Sciences. 2021; 14 (1):133-145.

Chicago/Turabian Style

Ismaila Muhammad; Mohd Y. Rafii; Muhamad Hazim Nazli; Shairul Izan Ramlee; Abdul Rahim Harun; Yusuff Oladosu. 2021. "Determination of lethal (LD) and growth reduction (GR)doses on acute and chronic gamma- irradiated Bambara groundnut [Vigna subterranea (L.) Verdc.]varieties." Journal of Radiation Research and Applied Sciences 14, no. 1: 133-145.

Review
Published: 01 January 2021 in BIOCELL
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Tharangani Welegama; Mohd Y. Rafii; Khairulmazmi Ahmad; Shairul I. Ramlee; Yusuff Oladosu. Development of high yield and tomato yellow leaf curl virus (TYLCV) resistance using conventional and molecular approaches: A review. BIOCELL 2021, 45, 1069 -1079.

AMA Style

Tharangani Welegama, Mohd Y. Rafii, Khairulmazmi Ahmad, Shairul I. Ramlee, Yusuff Oladosu. Development of high yield and tomato yellow leaf curl virus (TYLCV) resistance using conventional and molecular approaches: A review. BIOCELL. 2021; 45 (4):1069-1079.

Chicago/Turabian Style

Tharangani Welegama; Mohd Y. Rafii; Khairulmazmi Ahmad; Shairul I. Ramlee; Yusuff Oladosu. 2021. "Development of high yield and tomato yellow leaf curl virus (TYLCV) resistance using conventional and molecular approaches: A review." BIOCELL 45, no. 4: 1069-1079.

Preprint content
Published: 21 December 2020
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As a crop for the new millennium Bambara groundnut (Vigna subterranea L. Verdc.) considered as leading legumes in the tropical regions due to its versatile advantages. The main intent of this study was to find out the high yielding potential genotypes and considering these genotypes to develop pure lines for commercial cultivation in Malaysia. Considering the 14 qualitative and 27 quantitative traits of fifteen landraces the variation and genetic parameters namely, variability, heritability, genetic advance, characters association, and cluster matrix were determined. ANOVA revealed significant variation for all the agronomic traits (except plant height). Among the accessions, highly significant differences (P ≤ 0.01) were found for almost all the traits excluding fifty percent flowering date, seed length, seed width. The 16 traits out of the 27 quantitative traits had a coefficient of variation (CV) ≥ 20%. A positive and intermediate to perfect highly significant association (r = 0.23 to 1.00; P < 0.00) was found between yield and its related traits. The trait dry seed weight per plant (g) had the highest GCV = 59.91% and PCV = 59.57% whereas the trait fresh pod weight (99.55%), dry seed weight (98.86%), and yield (98.10%) were highly heritable. The genetic advance recorded the highest for dry seed weight (122.01%) and lowest (3.97%) for plant height. To validate the genetic disparity, an unweighted pair-group produce with arithmetic mean (UPGMA), principal component analysis (PCA), and Hˊ-index was performed considering 27 quantitative traits. The constructed dendrogram showed five distinct groups of accessions. Genotypes G2, G3, and G9 from Group IV consider as promising lines which gave 70.05% higher mean yield compared to grand mean yield (1180 kg ha-1) with desirable traits. Group II had a maximum number of accessions while group III and group V had one of each. However, findings declared that the availability of genetic variance will be beneficial for this crop improvement and plant breeders to prefer desirable traits in V. subterranea L. Verdc. for further breeding purposes.

ACS Style

Mahmudul Hasan Khan; Mohd Y. Rafii; Shairul Izan Ramlee; Mashitah Jusoh; Al- Mamun. Genetic Inconsistency Assessment and Selection Criteria of Bambara groundnut (Vigna subterranea L.Verdc.) Landraces for High Yield Revealed by Qualitative and Quantitative traits. 2020, 1 .

AMA Style

Mahmudul Hasan Khan, Mohd Y. Rafii, Shairul Izan Ramlee, Mashitah Jusoh, Al- Mamun. Genetic Inconsistency Assessment and Selection Criteria of Bambara groundnut (Vigna subterranea L.Verdc.) Landraces for High Yield Revealed by Qualitative and Quantitative traits. . 2020; ():1.

Chicago/Turabian Style

Mahmudul Hasan Khan; Mohd Y. Rafii; Shairul Izan Ramlee; Mashitah Jusoh; Al- Mamun. 2020. "Genetic Inconsistency Assessment and Selection Criteria of Bambara groundnut (Vigna subterranea L.Verdc.) Landraces for High Yield Revealed by Qualitative and Quantitative traits." , no. : 1.

Research article
Published: 21 December 2020 in Journal of Natural Fibers
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Understanding the genetic variability using different qualitative and quantitative traits plays a significant role in kenaf breeding program. To achieve this objective, a study was conducted on yield and yield components over two planting seasons to evaluate genetic diversity and heritability among kenaf mutant lines using multivariate analysis to identify superior genotype for future breeding program. Highly significant differences (p ≤ 0.01) was recorded among the qualitative and quantitative traits. Strong to moderately positive phenotypic correlation was observed between dry fiber weights for all the morphological traits except top diameter and days to flowering. The 31 studied genotypes were grouped into six major clusters based on the UPGMA dendrogram. Principal component analysis based on the correlation matrix of 15 quantitative traits showed that the first five components accounted for 85.16% of the total variation of the genotypes. Based on diversity pattern and morphophysiological performance, hybridization of group IV with group VI, or group III, group V and group I could be used to attain high vigor or heterosis with a comparative photo-insensitive variety with high fiber yield. Also, this evaluation could be useful in developing reliable selection criteria for future improvement in kenaf breeding programs.

ACS Style

Mohammad Al-Mamun; Mohd Rafii; Yusuff Oladosu; Azizah Binti Misran; Zulkarami Berahim; Zaiton Ahmad; Fatai Arolu; Mahmudul Hasan Khan. Genetic Diversity among Kenaf Mutants as Revealed by Qualitative and Quantitative Traits. Journal of Natural Fibers 2020, 1 -18.

AMA Style

Mohammad Al-Mamun, Mohd Rafii, Yusuff Oladosu, Azizah Binti Misran, Zulkarami Berahim, Zaiton Ahmad, Fatai Arolu, Mahmudul Hasan Khan. Genetic Diversity among Kenaf Mutants as Revealed by Qualitative and Quantitative Traits. Journal of Natural Fibers. 2020; ():1-18.

Chicago/Turabian Style

Mohammad Al-Mamun; Mohd Rafii; Yusuff Oladosu; Azizah Binti Misran; Zulkarami Berahim; Zaiton Ahmad; Fatai Arolu; Mahmudul Hasan Khan. 2020. "Genetic Diversity among Kenaf Mutants as Revealed by Qualitative and Quantitative Traits." Journal of Natural Fibers , no. : 1-18.

Research article
Published: 19 December 2020 in BioMed Research International
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Bambara groundnut (Vigna subterranea L. Verdc.) is considered an emerging crop for the future and known as a crop for the new millennium. The core intention of this research work was to estimate the variation of landraces of Bambara groundnut considering their 14 qualitative and 27 numerical traits, to discover the best genotype fitted in Malaysia. The findings of the ANOVA observed a highly significant variation ( p ≤ 0.01 ) for all the traits evaluated. There was a substantial variation (7.27 to 41.21%) coefficient value, and 14 out of the 27 numerical traits noted coefficient of variation CV ≥ 20 % . Yield (kg/ha) disclosed positively strong to perfect high significant correlation ( r = 0.75 to 1.00; p ≤ 0.001 ) with traits like fresh pod weight, dry pod weight, and dry seed weight. The topmost PCV and GCV values were estimated for biomass dry (41.09%) and fresh (40.53%) weight with high heritability (Hb) and genetic advance (GA) Hb = 95.19 %, GA = 80.57 % and Hb = 98.52 %, GA = 82.86 %, respectively. The topmost heritability was recorded for fresh pod weight (99.89%) followed by yield (99.75%) with genetic advance 67.95% and 62.03%, respectively. The traits with Hb ≥ 60 % and G A ≥ 20 % suggested the least influenced by the environment as well as governed by the additive genes and direct selection for improvement of such traits can be beneficial. To estimate the genetic variability among accessions, the valuation of variance components, coefficients of variation, heritability, and genetic advance were calculated. To authenticate the genetic inequality, an unweighted pair group produced with arithmetic mean (UPGMA) and principal component analysis was executed based on their measurable traits that could be a steadfast method for judging the degree of diversity. Based on the UPGMA cluster analysis, constructed five distinct clusters and 44 accessions from clusters II and IV consider an elite type of genotypes that produce more than one ton yield per hectare land with desirable traits. This study exposed an extensive disparity among the landraces and the evidence on genetic relatives will be imperative in using the existing germplasm for Bambara groundnut varietal improvement. Moreover, this finding will be beneficial for breeders to choose the desirable numerical traits of V. subterranea in their future breeding program.

ACS Style

Mahmudul Hasan Khan; Mohd Y. Rafii; Shairul Izan Ramlee; Mashitah Jusoh; Al Mamun. Genetic Variability, Heritability, and Clustering Pattern Exploration of Bambara Groundnut (Vigna subterranea L. Verdc) Accessions for the Perfection of Yield and Yield-Related Traits. BioMed Research International 2020, 2020, 1 -31.

AMA Style

Mahmudul Hasan Khan, Mohd Y. Rafii, Shairul Izan Ramlee, Mashitah Jusoh, Al Mamun. Genetic Variability, Heritability, and Clustering Pattern Exploration of Bambara Groundnut (Vigna subterranea L. Verdc) Accessions for the Perfection of Yield and Yield-Related Traits. BioMed Research International. 2020; 2020 ():1-31.

Chicago/Turabian Style

Mahmudul Hasan Khan; Mohd Y. Rafii; Shairul Izan Ramlee; Mashitah Jusoh; Al Mamun. 2020. "Genetic Variability, Heritability, and Clustering Pattern Exploration of Bambara Groundnut (Vigna subterranea L. Verdc) Accessions for the Perfection of Yield and Yield-Related Traits." BioMed Research International 2020, no. : 1-31.

Journal article
Published: 16 November 2020 in Agronomy
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This research was conducted to study the performance of biparental dura × pisifera (D × P) progenies and their parental genetic origins on fruit set and yield components. Twenty-four D × P progenies developed from 10 genetic origins were used for this study. Analysis of variance showed that there was genetic variability based on the evaluation of individual progenies. Deli Ulu Remis × Nigeria of progeny ECPHP500 recorded the highest bunch number (22.91), and fresh fruit bunch (184.62 kg palm−1 year−1) and Deli Banting dura × AVROS pisifera (ECPHP550) had the highest average bunch weight (10.36 kg palm−1 year−1). Progenies PK4674 (61.12%) and PK4465 (60.93%) had the highest fruit set, and the highest oil yield of 52.66 kg palm−1 year−1 was noticed by progeny PK4674. Estimation of variance components, coefficients of variation, heritability, and genetic gain were calculated to establish the genetic variability. To validate the genetic disparity among the progenies, an unweighted pair-group procedure with arithmetic mean (UPGMA) and principal component was employed based on their quantitative traits. Through the UPGMA and principal component, the 24 progenies were clustered into 7 clusters, whereas cluster V had the highest fruit set (60.62%) and cluster IV had the highest oil yield (43.71 kg palm−1 year−1). For oil palm tissue culture and breeding programs, progeny PK4674 will be more useful for developing planting materials of high oil yielding with stable performance. However, we recommend that future studies incorporate molecular studies with conventional breeding.

ACS Style

Senesie Swaray; Mohd Din Amiruddin; Mohd Y. Rafii; Syari Jamian; Mohd Firdaus Ismail; Momodu Jalloh; Marhalil Marjuni; Mohd Mustakim Mohamad; Oladosu Yusuff. Influence of Parental Dura and Pisifera Genetic Origins on Oil Palm Fruit Set Ratio and Yield Components in Their D × P Progenies. Agronomy 2020, 10, 1793 .

AMA Style

Senesie Swaray, Mohd Din Amiruddin, Mohd Y. Rafii, Syari Jamian, Mohd Firdaus Ismail, Momodu Jalloh, Marhalil Marjuni, Mohd Mustakim Mohamad, Oladosu Yusuff. Influence of Parental Dura and Pisifera Genetic Origins on Oil Palm Fruit Set Ratio and Yield Components in Their D × P Progenies. Agronomy. 2020; 10 (11):1793.

Chicago/Turabian Style

Senesie Swaray; Mohd Din Amiruddin; Mohd Y. Rafii; Syari Jamian; Mohd Firdaus Ismail; Momodu Jalloh; Marhalil Marjuni; Mohd Mustakim Mohamad; Oladosu Yusuff. 2020. "Influence of Parental Dura and Pisifera Genetic Origins on Oil Palm Fruit Set Ratio and Yield Components in Their D × P Progenies." Agronomy 10, no. 11: 1793.

Journal article
Published: 15 November 2020 in Plants
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Grafting is regarded as an integral component of sustainable vegetable production. It is important in the management of soil-borne diseases, and reports suggest that grafting with viable rootstocks can enhance crop growth and yield. This research was conducted using splices and cleft grafting techniques to investigate graft compatibility among varieties of high yielding eggplant scion (MCV1, MCV2, CCV1, CCV2, CCV3, NCV, and TCV) grafted onto wild rootstocks (MWR, BWR, and TWR) to study their morphophysiological and yield characteristics. High yielding scions grafted onto wild relative rootstocks were compared with two controls including self-grafted and non-grafted. All the scion had a high rate of germination (≥95%) and remarkable graft success (100%) was recorded in MCV1, MCV2, and TCV using the cleft techniques. Generally, the use of rootstocks resulted in higher total and marketable fruit yield compared to the non-grafted and self-grafted scion plants, respectively. In particular, MWR and TWR rootstock conferred the highest vigour to the scion, resulting in the highest values recorded for total and marketable fruit yield, number of fruits per plant and average fruit weight. A similar result was obtained in fruit length and diameter, where long and wide fruits were observed in scions grafted onto MWR and TWR rootstocks, respectively. Grafting of high yielding eggplant scion onto resistant MWR, BWR and TWR eggplant rootstock was found to be beneficial for eggplant cultivation. The remarkable compatibility and vigour of the rootstock with scion led to the improvement in total and marketable yield of the fruits. As such, it can be concluded that the use of wild relative rootstocks of eggplant species can be a valuable method of improving eggplant production.

ACS Style

Ibrahim Musa; Mohd Y. Rafii; Khairulmazmi Ahmad; Shairul Izan Ramlee; Muhammad Asyraf Md Hatta; Yusuff Oladosu; Isma’Ila Muhammad; Samuel Chibuike Chukwu; Nur Nadzirah Mat Sulaiman; Arolu Fatai Ayanda; Jamilu Halidu. Effects of Grafting on Morphophysiological and Yield Characteristic of Eggplant (Solanum melongena L.) Grafted onto Wild Relative Rootstocks. Plants 2020, 9, 1583 .

AMA Style

Ibrahim Musa, Mohd Y. Rafii, Khairulmazmi Ahmad, Shairul Izan Ramlee, Muhammad Asyraf Md Hatta, Yusuff Oladosu, Isma’Ila Muhammad, Samuel Chibuike Chukwu, Nur Nadzirah Mat Sulaiman, Arolu Fatai Ayanda, Jamilu Halidu. Effects of Grafting on Morphophysiological and Yield Characteristic of Eggplant (Solanum melongena L.) Grafted onto Wild Relative Rootstocks. Plants. 2020; 9 (11):1583.

Chicago/Turabian Style

Ibrahim Musa; Mohd Y. Rafii; Khairulmazmi Ahmad; Shairul Izan Ramlee; Muhammad Asyraf Md Hatta; Yusuff Oladosu; Isma’Ila Muhammad; Samuel Chibuike Chukwu; Nur Nadzirah Mat Sulaiman; Arolu Fatai Ayanda; Jamilu Halidu. 2020. "Effects of Grafting on Morphophysiological and Yield Characteristic of Eggplant (Solanum melongena L.) Grafted onto Wild Relative Rootstocks." Plants 9, no. 11: 1583.

Journal article
Published: 22 October 2020 in Plants
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The most vital aspect of marker-assisted backcross breeding is the recurrent parent genome recovery. This enables the selection of only parents with recovered recipient/recurrent parent genome in addition to the targeted genes. The recurrent parent genome recovery (RPGR) ensures that non-desirable genomic segments are removed while the gene of interest is sustained in the recombined progenies without further segregations. This work was aimed at quantifying the RPGR of backcross populations with introgression of bacterial leaf blight resistance genes. Putra-1, a Malaysian elite variety, high yielding with inherent resistance to blast but susceptible to bacterial leaf blight (BLB), was crossed with IRBB60 which is resistant to BLB disease. The IRBB60 has four Xoo resistance genes—Xa4, xa5, xa13 and Xa21. Tightly linked polymorphic functional and SSR markers were used for foreground selection at every stage of backcrossing to select progenies with introgressed target genes. Background selection was done to quantify the percentage of RPGR in the selected lines using 79 confirmed polymorphic microsatellites. Result obtained showed that the percentage of RPGR was 80.11% at BC1F1, 95.30% at BC2F1 and 95.9% at BC2F2. The introgression of Xa4, xa5, xa13 and Xa21 Xoo resistance genes were faster through the adopted marker-assisted backcross breeding compared to what could be obtained through conventional breeding. All the 16 selected lines displayed resistance to BLB with three lines showing high resistance to the disease. The blast resistance contained in the genetic background of Putra-1 was also sustained in all the selected lines. The newly developed lines were recommended as new rice varieties for commercial cultivation.

ACS Style

Samuel Chukwu; Mohd Rafii; Shairul Ramlee; Siti Ismail; Yusuff Oladosu; Isma’Ila Muhammad; Ibrahim Musa; Muideen Ahmed; Muhammed Jatto; Bashir Yusuf. Recovery of Recurrent Parent Genome in a Marker-Assisted Backcrossing Against Rice Blast and Blight Infections Using Functional Markers and SSRs. Plants 2020, 9, 1411 .

AMA Style

Samuel Chukwu, Mohd Rafii, Shairul Ramlee, Siti Ismail, Yusuff Oladosu, Isma’Ila Muhammad, Ibrahim Musa, Muideen Ahmed, Muhammed Jatto, Bashir Yusuf. Recovery of Recurrent Parent Genome in a Marker-Assisted Backcrossing Against Rice Blast and Blight Infections Using Functional Markers and SSRs. Plants. 2020; 9 (11):1411.

Chicago/Turabian Style

Samuel Chukwu; Mohd Rafii; Shairul Ramlee; Siti Ismail; Yusuff Oladosu; Isma’Ila Muhammad; Ibrahim Musa; Muideen Ahmed; Muhammed Jatto; Bashir Yusuf. 2020. "Recovery of Recurrent Parent Genome in a Marker-Assisted Backcrossing Against Rice Blast and Blight Infections Using Functional Markers and SSRs." Plants 9, no. 11: 1411.