Blackberry (Rubus L.) cultivation in Kazakhstan is constrained by the limited availability of certified planting material and the absence of standardized micropropagation protocols adapted to local conditions. This study aimed to optimize the key stages of in vitro culture for the cultivars ‘Natchez’, ‘Black Magic’, ‘Osage’, and ‘Heaven Can Wait’, including explant sterilization, culture initiation, shoot multiplication, and acclimatization. A sequential sterilization scheme using 70% ethanol followed by 1% sodium hypochlorite ensured high explant survival. Shoot initiation was most efficient on MS medium supplemented with 0.1 mg/L BAP, whereas multiplication was enhanced by 0.5 mg/L BAP and 0.1 mg/L GA₃. In the subsequent rooting stage, microcuttings formed stable root systems under ex vitro conditions in agroboxes, confirming that the optimized protocol ensured not only high survival during initiation but also a successful transition to the rooting phase, which is essential for further acclimatization. During ex vitro acclimatization, the application of humic acid, nanosilicon, or succinic acid improved survival under agrobox microventilation. The developed approach provides a reliable framework for producing healthy, adapted plants of the evaluated cultivars and contributes to establishing domestic propagation systems for reducing reliance on imported planting material.

The article presents a study on optimizing the conditions for micropropagation of three varieties of Amelanchier canadensis in vitro. It was found that modification of the Murashige and Skoog nutrient medium by introducing 6-benzylaminopurine (BAP) in various concentrations has a differentiated effect on the morphogenetic activity of explants. The most effective combination for Martin and Slate varieties was the use of BAP at a concentration of 1.0 mg/l and GA₃ – 0.5 mg/l, while for the Northline variety the optimal concentration was 0.5 mg/l BAP and 0.5 mg/l GA₃. Increasing the BAP concentration above 1.0 mg/l led to a decrease in both the length and the number of microshoots. The results obtained can be applied to large-scale propagation and conservation of these varieties of serviceberry.