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J. Plácido
VEDAS Corporación de Investigación e Innovación (VEDAS CII), Cl 8 B 65-261, Medellín 050024, Colombia

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Short Biography

Jersson Placido is a biological engineer and a master’s in biotechnology from the National University of Colombia and a doctorate in biological engineering from Texas A&M University. Dr. Plácido has worked as a researcher at Texas A&M University, University of Southampton and Swansea University and currently he is serving as research director at VEDAS CII. Dr. Placido is an expert in industrial and environmental biotechnology, nanotechnology and renewable energy. Dr. Plácido has participated in different projects in the transformation of agro-industrial waste into advanced nanomaterials and bioenergy and in the development of BioRefineries for the production of volatile fatty acids and biofuels from food and agro-industrial waste, additionally, he is an expert in the application of enzymatic processes for the degradation of pollutants in aqueous systems.

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Journal article
Published: 24 June 2021 in Processes
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Biochar-derived C-Dots from Picea, Molinia caerulea and Elaeis guineensis were synthesized through a hydrothermal process, and their physicochemical and optical characteristics and environmental effects were compared. These C-Dots were characterized by techniques such as Attenuated Total Reflection–Fourier Transform Infrared (ATR-FTIR), UV-Vis spectrophotometry, fluorescence spectroscopy, dynamic light scattering (DLS), Z potential, and High-Resolution Transmission Electronical Microscopy (HR-TEM). The ecotoxicity tests were performed using the Microtox™ test, making this study one of the few that use this method. The C-Dots from Molinia caerulea showed the best quantum yield (QY) of 8.39% and moderate ecotoxicity, while Elaeis guineensis has the lowest QY (2.31%) but with zero toxicity. Furthermore, the C-Dots from Picea presents good optical properties but showed high toxicity and limits its use. Finally, all C-Dots showed functional groups that could be biofunctionalized with biomolecules, especially C-Dots from Molinia caerulea and Elaeis guineensis show potential for use in the development of optical biosensors.

ACS Style

Kaory Barrientos; Maria Gaviria; Juan Arango; Jersson Placido; Sandra Bustamante; Martha Londoño; Marisol Jaramillo. Synthesis, Characterization and Ecotoxicity Evaluation of Biochar-Derived Carbon Dots from Spruce Tree, Purple Moor-Grass and African Oil Palm. Processes 2021, 9, 1095 .

AMA Style

Kaory Barrientos, Maria Gaviria, Juan Arango, Jersson Placido, Sandra Bustamante, Martha Londoño, Marisol Jaramillo. Synthesis, Characterization and Ecotoxicity Evaluation of Biochar-Derived Carbon Dots from Spruce Tree, Purple Moor-Grass and African Oil Palm. Processes. 2021; 9 (7):1095.

Chicago/Turabian Style

Kaory Barrientos; Maria Gaviria; Juan Arango; Jersson Placido; Sandra Bustamante; Martha Londoño; Marisol Jaramillo. 2021. "Synthesis, Characterization and Ecotoxicity Evaluation of Biochar-Derived Carbon Dots from Spruce Tree, Purple Moor-Grass and African Oil Palm." Processes 9, no. 7: 1095.

Review
Published: 08 January 2021 in Viruses
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Virus research has advanced significantly since the discovery of the tobacco mosaic virus (TMV), the characterization of its infection mechanisms and the factors that determine their pathogenicity. However, most viral research has focused on pathogenic viruses to humans, animals and plants, which represent only a small fraction in the virosphere. As a result, the role of most viral genes, and the mechanisms of coevolution between mutualistic viruses, their host and their environment, beyond pathogenicity, remain poorly understood. This review focuses on general aspects of viruses that interact with extremophile organisms, characteristics and examples of mechanisms of adaptation. Finally, this review provides an overview on how knowledge of extremophile viruses sheds light on the application of new tools of relevant use in modern molecular biology, discussing their value in a biotechnological context.

ACS Style

Jose F. Gil; Victoria Mesa; Natalia Estrada-Ortiz; Mauricio Lopez-Obando; Andrés Gómez; Jersson Plácido. Viruses in Extreme Environments, Current Overview, and Biotechnological Potential. Viruses 2021, 13, 81 .

AMA Style

Jose F. Gil, Victoria Mesa, Natalia Estrada-Ortiz, Mauricio Lopez-Obando, Andrés Gómez, Jersson Plácido. Viruses in Extreme Environments, Current Overview, and Biotechnological Potential. Viruses. 2021; 13 (1):81.

Chicago/Turabian Style

Jose F. Gil; Victoria Mesa; Natalia Estrada-Ortiz; Mauricio Lopez-Obando; Andrés Gómez; Jersson Plácido. 2021. "Viruses in Extreme Environments, Current Overview, and Biotechnological Potential." Viruses 13, no. 1: 81.

Preprint content
Published: 05 November 2020
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In this research, operational parameters were assessed to optimise the production of volatile fatty acids (VFA) from artisanal fish waste (FW) using mixed-culture fermentation. The experiments were performed using a randomised block design with pH and Inoculum/Substrate (I/S) ratio as factors with three pH levels (5, 7 and 9) and five I/S ratios levels (0.20, 0.15, 0.10, 0.05, 0.00) with VFA production, total ammonia nitrogen (TAN), biogas and soluble chemical oxygen demand (sCOD) as response variables. The results indicated that pH and I/S ratio significantly influenced VFA production and the other variables evaluated. The highest VFA concentration (54.5 g/L) was obtained at pH 7 and with an I/S ratio of 0.05. These conditions were also optimal for TAN and sCOD. External pH control was proved as unnecessary as the highest VFA production (70.3 g VFA/L) was obtained without pH control. In these conditions, the fermentation broth was able to maintain its pH between 6.6 and 7.7. The VFA profile had acetic (35%) and butyric (32%) acids as the dominant species and propionic, iso-valeric and iso-butyric were minor species (< 10%). An economic assessment evaluating the establishment of a FW VFA biorefinery in Tumaco, Colombia was performed including capital, operational and labour cost, discounted payback period and cost curves. These results evidenced the potential of artisanal FW as a raw material for producing high concentrations of VFA and smooth the path for the establishment of a VFA and biogas biorefinery from FW.

ACS Style

Luz Stella Cadavid-Rodríguez; Viviana E. Castro-López; Jersson Placido. Evaluation of optimal fermentation conditions for volatile fatty acids production from artisanal fish waste. 2020, 1 .

AMA Style

Luz Stella Cadavid-Rodríguez, Viviana E. Castro-López, Jersson Placido. Evaluation of optimal fermentation conditions for volatile fatty acids production from artisanal fish waste. . 2020; ():1.

Chicago/Turabian Style

Luz Stella Cadavid-Rodríguez; Viviana E. Castro-López; Jersson Placido. 2020. "Evaluation of optimal fermentation conditions for volatile fatty acids production from artisanal fish waste." , no. : 1.

Preprint content
Published: 24 August 2020
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In this research, operational parameters were assessed to optimise the production of volatile fatty acids (VFA) from artisanal fish waste (FW) using mixed-culture fermentation. The experiments were performed using a randomised block design with pH and Inoculum/Substrate (I/S) ratio as factors with three pH levels (5, 7 and 9) and five I/S ratios levels (0.20, 0.15, 0.10, 0.05, 0.00) with VFA production, total ammonia nitrogen (TAN), biogas and soluble chemical oxygen demand (sCOD) as response variables. The results indicated that pH and I/S ratio significantly influenced VFA production and the other variables evaluated. The highest VFA concentration (54.5 g/L) was obtained at pH 7 and with an I/S ratio of 0.05. These conditions were also optimal for TAN and sCOD. External pH control was proved as unnecessary as the highest VFA production (70.3 g VFA/L) was obtained without pH control. In these conditions, the fermentation broth was able to maintain its pH between 6.6 and 7.7. The VFA profile had acetic (35%) and butyric (32%) acids as the dominant species, however, propionic, iso-valeric and iso-butyric were also produced in small amounts (< 10%). These results evidence the potential of artisanal FW as a raw material for producing high concentrations of VFA and smooth the path for the establishment of a VFA and biogas biorefinery from artisanal FW.

ACS Style

Luz Stella Cadavid-Rodríguez; Viviana E. Castro-López; Jersson Placido. Evaluation of optimal fermentation conditions for volatile fatty acids production from artisanal fish waste. 2020, 1 .

AMA Style

Luz Stella Cadavid-Rodríguez, Viviana E. Castro-López, Jersson Placido. Evaluation of optimal fermentation conditions for volatile fatty acids production from artisanal fish waste. . 2020; ():1.

Chicago/Turabian Style

Luz Stella Cadavid-Rodríguez; Viviana E. Castro-López; Jersson Placido. 2020. "Evaluation of optimal fermentation conditions for volatile fatty acids production from artisanal fish waste." , no. : 1.

Journal article
Published: 27 July 2019 in Fuel Processing Technology
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Three types of biochar (microalgae, rice straw and sorghum straw) from biomass thermal conversion production were tested for producing biochar-derived carbonaceous nanomaterials (BCN). BCN were obtained after using chemical depolymerisation and solvent extraction, NanoRefinery process. Microalgae biochar-derived carbonaceous nanomaterials (MAB-CN), rice straw biochar-derived carbonaceous nanomaterials (RSB-CN) and sorghum straw biochar-derived carbonaceous nanomaterials (SSB-CN) were characterised using spectroscopic and microscopic techniques. This characterisation evidenced significant differences among the three BCN with MAB-CN exhibiting greater structural differences compared to RSB-CN and SSB-CN. Biocompatibility, cellular uptake, and cellular localisation were evaluated using three yeast species, Saccharomyces cerevisiae, Candida albicans, and Yarrowia lipolytica. While all BCN were biocompatible, the degree of biocompatibility for each species was dependent on pH, BCN concentration and BCN type. Additionally, BCN were evaluated as transducers for the detection of 12 heavy metal ions. MAB-CN, RSB-CN, and SSB-CN had different responses to the 12 heavy metal ions. The SSB-CN/Cu (II) and the MAB-CN/Zn (II) combinations evidenced selectivity over the other metal ions with these combinations having limits of detection of 0.0125 μM and 9 μM, respectively. The results from this research pave the way for BCN novel applications for bioimaging and heavy metal ions sensing probes.

ACS Style

J. Plácido; S. Bustamante-López; K.E. Meissner; D.E. Kelly; S.L. Kelly. Comparative study of the characteristics and fluorescent properties of three different biochar derived‑carbonaceous nanomaterials for bioimaging and heavy metal ions sensing. Fuel Processing Technology 2019, 196, 106163 .

AMA Style

J. Plácido, S. Bustamante-López, K.E. Meissner, D.E. Kelly, S.L. Kelly. Comparative study of the characteristics and fluorescent properties of three different biochar derived‑carbonaceous nanomaterials for bioimaging and heavy metal ions sensing. Fuel Processing Technology. 2019; 196 ():106163.

Chicago/Turabian Style

J. Plácido; S. Bustamante-López; K.E. Meissner; D.E. Kelly; S.L. Kelly. 2019. "Comparative study of the characteristics and fluorescent properties of three different biochar derived‑carbonaceous nanomaterials for bioimaging and heavy metal ions sensing." Fuel Processing Technology 196, no. : 106163.

Journal article
Published: 25 June 2019 in Science of The Total Environment
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This article describes an efficient method, combining chemical oxidation and acetone extraction, to produce carbonaceous nanomaterials from dairy manure biochar. The optical and mechanical properties are similar to methods previously reported carbonaceous nanomaterials from biomass. Our novel process cuts the processing time in half and drastically reduces the energy input required. The acetone extraction produced 10 fractions with dairy manure biochar-derived carbonaceous nanomaterials (DMB–CNs). The fraction with the carbonaceous nanomaterials, DMB–CN-E1, with highest fluorescence was selected for in-depth characterisation and for initial testing across a range of applications. DMB–CN-E1 was characterised using atomic force microscope, electrophoresis, and spectrophotometric methods. DMB–CN-E1 exhibited a lateral dimension between 11 and 28 nm, a negative charge, and excitation/emission maxima at 337/410 nm, respectively. The bioimaging potential of DMB–CN-E1 evidenced different locations and different interactions with the cellular models evaluated. DMB–CN-E1 was quenched by several heavy metal ions showing a future application of these materials in heavy metal ion detection and/or removal. The demonstrated capabilities in bioimaging and environmental sensing create the opportunity for generating added-value nanomaterials (NanoRefinery) from dairy manure biochar gasification and, thus, increasing the economic viability of gasification plants.

ACS Style

J. Plácido; S. Bustamante-López; K.E. Meissner; D.E. Kelly; S.L. Kelly. NanoRefinery of carbonaceous nanomaterials: Complementing dairy manure gasification and their applications in cellular imaging and heavy metal sensing. Science of The Total Environment 2019, 689, 10 -20.

AMA Style

J. Plácido, S. Bustamante-López, K.E. Meissner, D.E. Kelly, S.L. Kelly. NanoRefinery of carbonaceous nanomaterials: Complementing dairy manure gasification and their applications in cellular imaging and heavy metal sensing. Science of The Total Environment. 2019; 689 ():10-20.

Chicago/Turabian Style

J. Plácido; S. Bustamante-López; K.E. Meissner; D.E. Kelly; S.L. Kelly. 2019. "NanoRefinery of carbonaceous nanomaterials: Complementing dairy manure gasification and their applications in cellular imaging and heavy metal sensing." Science of The Total Environment 689, no. : 10-20.

Journal article
Published: 24 June 2019 in Science of The Total Environment
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This article focuses on implementing multivariate analysis to evaluate biochar-derived carbonaceous nanomaterials (BCN) from three different feedstocks for the detection and differentiation of heavy metal ions in aqueous systems. The BCN were produced from dairy manure, rice straw and sorghum straw biochar using our NanoRefinery process. The NanoRefinery process transforms biochar into advanced nanomaterials using depolymerisation/chemical oxidation and purification of nanomaterials using solvent extraction. Dairy manure biochar-derived carbonaceous nanomaterials (DMB-CN), rice straw biochar-derived carbonaceous nanomaterials (RSB-CN) and sorghum straw biochar-derived carbonaceous nanomaterials (SSB-CN) were utilised as probes for the evaluation of their fluorescent properties and the detection of heavy metal ions. The BCN fluorescence quenching and fluorescence recovery was tested with lead (Pb2+), nickel (Ni2+), copper (Cu2+) and mercury (Hg2+). Principal component analysis (PCA) and discriminant analysis were used to differentiate among heavy metal ions in water samples. The BCN from different feedstocks had different characteristics and produced different interactions with heavy metal ions. DMB-CN had the highest quenching for Hg2+ and Ni2+ while SSB-CN and RSB-CN responded best to Cu2+ and Pb2+, respectively. The fluorescence quenching was modelled using linear and empirical functions. PCA and discriminant analysis used the quenching measurements to differentiate heavy metal ions in aqueous system. A key result was that the discriminant analysis had a 100% accuracy to detect Pb2+, 66% for Ni2+ and Cu2+, and 33% for Hg2+. This study has shown that biochar-derived carbonaceous nanomaterials could be used in heavy metal ions sensing applications. This is the first step in the development of a fast and accurate method for the detection of heavy metal ions in waters using environmentally friendly BCN.

ACS Style

J. Plácido; S. Bustamante López; K.E. Meissner; D.E. Kelly; S.L. Kelly. Multivariate analysis of biochar-derived carbonaceous nanomaterials for detection of heavy metal ions in aqueous systems. Science of The Total Environment 2019, 688, 751 -761.

AMA Style

J. Plácido, S. Bustamante López, K.E. Meissner, D.E. Kelly, S.L. Kelly. Multivariate analysis of biochar-derived carbonaceous nanomaterials for detection of heavy metal ions in aqueous systems. Science of The Total Environment. 2019; 688 ():751-761.

Chicago/Turabian Style

J. Plácido; S. Bustamante López; K.E. Meissner; D.E. Kelly; S.L. Kelly. 2019. "Multivariate analysis of biochar-derived carbonaceous nanomaterials for detection of heavy metal ions in aqueous systems." Science of The Total Environment 688, no. : 751-761.

Journal article
Published: 22 May 2019 in Sustainable Energy Technologies and Assessments
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The potential of biogas production from fish waste as source of renewable energy for fishermen communities was evaluated. Four different fish waste concentrations (1%, 1.5%, 2% and 2.5% total solids (TS)) were digested during 28 days at mesophilic conditions. Biochemical Methane Potential (BMP), volatile fatty acids (VFA) concentration and ammonia concentration were analysed during the experiment. Energy production and economic projections were performed to estimate the number of families that can benefit from the biogas production in Tumaco, Colombia. The 1% TS had the highest BMP (464.5 mL CH4/g VS) and the lowest VFA production (2515 mg/L); in contrast, the 2.5% TS had the highest VFA production (11302 mg/L) and the lowest methane production (206.86 mL CH4/g VS). The treatments with 1.5%, 2% and 2.5% TS exhibited diauxic growth as result of different solubilisation rates in the fish waste components. The energetic and economic analyses estimated a yearly energy production of 489 MWh, which can satisfy the electric energy consumption or the cooking energy demand of 230 fishermen families. The results showed that biogas production from fish waste is a viable and sustainable alternative to adequately manage this material and provide renewable energy to fishermen communities.

ACS Style

L.S. Cadavid-Rodríguez; M.A. Vargas-Muñoz; J. Plácido. Biomethane from fish waste as a source of renewable energy for artisanal fishing communities. Sustainable Energy Technologies and Assessments 2019, 34, 110 -115.

AMA Style

L.S. Cadavid-Rodríguez, M.A. Vargas-Muñoz, J. Plácido. Biomethane from fish waste as a source of renewable energy for artisanal fishing communities. Sustainable Energy Technologies and Assessments. 2019; 34 ():110-115.

Chicago/Turabian Style

L.S. Cadavid-Rodríguez; M.A. Vargas-Muñoz; J. Plácido. 2019. "Biomethane from fish waste as a source of renewable energy for artisanal fishing communities." Sustainable Energy Technologies and Assessments 34, no. : 110-115.

Journal article
Published: 21 February 2019 in Biocatalysis and Agricultural Biotechnology
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In the present work, immobilisation of Leptosphaerulina sp., a Colombian native fungus, improved the biotransformation of pollutants (Remazol black 5 (RB5) dye and cephadroxyl (CPD) antibiotic) in aqueous systems. Four different natural immobilisation matrices (charcoal, luffa sponge, wood chips and cork) were tested in order to select the most suitable for Leptosphaerulina sp. biomass augmentation. Luffa sponge was selected qualitatively as the most appropriate material for the immobilisation of Leptosphaerulina sp. CPD and RB5 biotransformation was performed with immobilised and suspended Leptosphaerulina sp. cultures on luffa sponge. The luffa sponge-immobilised fungus exhibited a considerable removal of CPD (∼100%) and RB5 (91.9%). The luffa sponge-immobilised Leptosphaerulina sp. achieved a higher CPD removal than the suspended cultures (∼100% vs 94.4%, respectively, on day 15). RB5 experiments revealed a higher removal (91.9% for immobilised fungus vs 87% for suspended fungus, on day 15) and a faster transformation of RB5 in luffa sponge-immobilised cultures than that of free cultures (26.3 decolourisation % per day for immobilised cultures vs 18.2 decolourisation % per day for suspended cultures). Additionally, luffa sponge immobilisation also improved Leptosphaerulina sp. production of laccase (Lac) and manganese peroxidase (MnP) (e.g. at day 3, Lac and MnP in immobilised culture were 84% and 76%, respectively, higher than suspended culture during CDP removal, and 83% and 5% in bio-treatment of RB5). These results evidenced the potential of Luffa sponge-immobilised Leptosphaerulina sp. as a strategy to enhance the biodegradation of recalcitrant compounds, to facilitate biomass recycling and to be used in the process scale‒up.

ACS Style

María Susana Pérez Grisales; Marian Castrillón-Tobón; Ledys S. Copete-Pertuz; Jersson Placido; Amanda L. Mora-Martínez. Biotransformation of the antibiotic agent cephadroxyl and the synthetic dye Reactive Black 5 by Leptosphaerulina sp. immobilised on Luffa (Luffa cylindrica) sponge. Biocatalysis and Agricultural Biotechnology 2019, 18, 101051 .

AMA Style

María Susana Pérez Grisales, Marian Castrillón-Tobón, Ledys S. Copete-Pertuz, Jersson Placido, Amanda L. Mora-Martínez. Biotransformation of the antibiotic agent cephadroxyl and the synthetic dye Reactive Black 5 by Leptosphaerulina sp. immobilised on Luffa (Luffa cylindrica) sponge. Biocatalysis and Agricultural Biotechnology. 2019; 18 ():101051.

Chicago/Turabian Style

María Susana Pérez Grisales; Marian Castrillón-Tobón; Ledys S. Copete-Pertuz; Jersson Placido; Amanda L. Mora-Martínez. 2019. "Biotransformation of the antibiotic agent cephadroxyl and the synthetic dye Reactive Black 5 by Leptosphaerulina sp. immobilised on Luffa (Luffa cylindrica) sponge." Biocatalysis and Agricultural Biotechnology 18, no. : 101051.

Journal article
Published: 27 November 2018 in Science of The Total Environment
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This research seeks a coupled solution for managing the large amounts of biochar produced by microalgae biofuel production, and the necessity for novel, economic and accurate heavy metal sensing methods. Therefore, this study evaluated the transformation of microalgae biochar (MAB) into carbon dots (Cdots) and their subsequent application as heavy metal ion sensors in aqueous systems. The experimental phase included the transformation of MAB into microalgae biochar-derived carbon dots (MAB–Cdots), MAB–Cdot characterisation and the evaluation of the MAB–Cdots as transducers for the detection of four heavy metal ions (Pb2+, Cu2+, Cd2+, and Ni2+). MAB–Cdot fluorescence was stable over a wide range of pH and resistant to photo-bleaching, making them suitable as fluorescence probes. The MAB–Cdot fluorescence was quenched by all of the metal ions and displayed different quenching levels. Depending upon the ions involved, MAB–Cdots were used to detect the presence of heavy metal ions from concentrations of 0.012 μM up to 2 mM by measuring the reduction in fluorescence intensity. Neutral and slightly alkaline pHs were optimal for Cu2+ Ni2+ and Pb2+ heavy metal quenching. To quantify the concentration of the heavy metal ions, linear and logarithmic functions were used to model the MAB–Cdot fluorescence quenching. The sensing mechanism was determined to be reversible and purely collisional with some fluorophores less accessible than the others. This work demonstrated the ability to produce Cdots from microalgae biochar, examined their application as a transducer for detecting heavy metal ions in aqueous systems and paves the way for novel sensing systems using MAB-Cdots.

ACS Style

J. Plácido; S. Bustamante-López; K.E. Meissner; D.E. Kelly; S.L. Kelly. Microalgae biochar-derived carbon dots and their application in heavy metal sensing in aqueous systems. Science of The Total Environment 2018, 656, 531 -539.

AMA Style

J. Plácido, S. Bustamante-López, K.E. Meissner, D.E. Kelly, S.L. Kelly. Microalgae biochar-derived carbon dots and their application in heavy metal sensing in aqueous systems. Science of The Total Environment. 2018; 656 ():531-539.

Chicago/Turabian Style

J. Plácido; S. Bustamante-López; K.E. Meissner; D.E. Kelly; S.L. Kelly. 2018. "Microalgae biochar-derived carbon dots and their application in heavy metal sensing in aqueous systems." Science of The Total Environment 656, no. : 531-539.

Journal article
Published: 29 July 2018 in Science of The Total Environment
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This work investigated fungal co–culture as inducer of ligninolytic enzymes and decolourising activity in the Colombian strain Leptosphaerulina sp., an ascomycete white-rot fungus isolated from lignocellulosic material. Aspergillus niger, Aspergillus fumigatus, Aspergillus terreus, Trichoderma viride, Fusarium sp. and Penicillium chrysogenum were tested as Leptosphaerulina sp. inducers. The best fungal combinations in terms of enzyme production, fungal growth and decolourising activity were selected from solid media experiments. Response surface methodology (RSM) was utilised to optimise enzyme production and decolourising activity in liquid media. Solid media assays evidenced T. viride and A. terreus as the best Leptosphaerulina sp. inducers. The RSM identified a triple co–culture inoculated with T. viride (1000 μL) and A. terreus (1000 μL) into a 7–day culture of Leptosphaerulina sp. as the best treatment. This triple combination significantly improved ligninolytic enzymes production and Reactive Black 5 dye removal when compared to the Leptosphaerulina sp. monoculture and previously used chemical inducers. These results demonstrated the potential of fungal co–culture as an environmentally–friendly method to enhance Leptosphaerulina sp. enzymes production and decolourising activity.

ACS Style

Ledys S. Copete-Pertuz; Felipe Alandete-Novoa; Jersson Plácido; Guillermo A. Correa-Londoño; Amanda L. Mora-Martínez. Enhancement of ligninolytic enzymes production and decolourising activity in Leptosphaerulina sp. by co–cultivation with Trichoderma viride and Aspergillus terreus. Science of The Total Environment 2018, 646, 1536 -1545.

AMA Style

Ledys S. Copete-Pertuz, Felipe Alandete-Novoa, Jersson Plácido, Guillermo A. Correa-Londoño, Amanda L. Mora-Martínez. Enhancement of ligninolytic enzymes production and decolourising activity in Leptosphaerulina sp. by co–cultivation with Trichoderma viride and Aspergillus terreus. Science of The Total Environment. 2018; 646 ():1536-1545.

Chicago/Turabian Style

Ledys S. Copete-Pertuz; Felipe Alandete-Novoa; Jersson Plácido; Guillermo A. Correa-Londoño; Amanda L. Mora-Martínez. 2018. "Enhancement of ligninolytic enzymes production and decolourising activity in Leptosphaerulina sp. by co–cultivation with Trichoderma viride and Aspergillus terreus." Science of The Total Environment 646, no. : 1536-1545.

Journal article
Published: 01 July 2018 in Science of The Total Environment
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In this work, Leptosphaerulina sp. (a Colombian native fungus) significantly removed three Isoxazolyl-Penicillin antibiotics (IP): oxacillin (OXA, 16000 μg L−1), cloxacillin (CLX, 17500 μg L−1) and dicloxacillin (DCX, 19000 μg L−1) from water. The biological treatment was performed at pH 5.6, 28 °C, and 160 rpm for 15 days. The biotransformation process and lack of toxicity of the final solutions (antibacterial activity (AA) and cytotoxicity) were tested. The role of enzymes in IP removal was analysed through in vitro studies with enzymatic extracts (crude and pre-purified) from Leptosphaerulina sp., commercial enzymes and enzymatic inhibitors. Furthermore, the applicability of mycoremediation process to a complex matrix (simulated hospital wastewater) was evaluated. IP were considerably abated by the fungus, OXA was the fastest degraded (day 6), followed by CLX (day 7) and DCX (day 8). Antibiotics biodegradation was associated to laccase and versatile peroxidase action. Assays using commercial enzymes (i.e. laccase from Trametes versicolor and horseradish peroxidase) and inhibitors (EDTA, NaCl, sodium acetate, manganese (II) ions) confirmed the significant role of enzymatic transformation. Whereas, biomass sorption was not an important process in the antibiotics elimination. Evaluation of AA against Staphylococcus aureus ATCC 6538 revealed that Leptosphaerulina sp. also eliminated the AA. In addition, the cytotoxicity assay (MTT) on the HepG2 cell line demonstrated that the IP final solutions were non-toxic. Finally, Leptosphaerulina sp. eliminated OXA and its AA from synthetic hospital wastewater at 6 days. All these results evidenced the potential of Leptosphaerulina sp. mycoremediation as a novel environmentally friendly process for the removal of IP from aqueous systems.

ACS Style

Ledys S. Copete-Pertuz; Jersson Placido; Efraim A. Serna-Galvis; Ricardo A. Torres-Palma; Amanda Mora. Elimination of Isoxazolyl-Penicillins antibiotics in waters by the ligninolytic native Colombian strain Leptosphaerulina sp. considerations on biodegradation process and antimicrobial activity removal. Science of The Total Environment 2018, 630, 1195 -1204.

AMA Style

Ledys S. Copete-Pertuz, Jersson Placido, Efraim A. Serna-Galvis, Ricardo A. Torres-Palma, Amanda Mora. Elimination of Isoxazolyl-Penicillins antibiotics in waters by the ligninolytic native Colombian strain Leptosphaerulina sp. considerations on biodegradation process and antimicrobial activity removal. Science of The Total Environment. 2018; 630 ():1195-1204.

Chicago/Turabian Style

Ledys S. Copete-Pertuz; Jersson Placido; Efraim A. Serna-Galvis; Ricardo A. Torres-Palma; Amanda Mora. 2018. "Elimination of Isoxazolyl-Penicillins antibiotics in waters by the ligninolytic native Colombian strain Leptosphaerulina sp. considerations on biodegradation process and antimicrobial activity removal." Science of The Total Environment 630, no. : 1195-1204.

Original article
Published: 05 May 2018 in Biomass Conversion and Biorefinery
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The volatile fatty acid (VFA) production potential from animal blood and the factors affecting this process were investigated in this study. In order to simulate an industrial process different operation modes, batch, fed batch and semi-continuous, were also evaluated. Due to high ammonia concentration in fermentation broth, VFA concentration up to 100 g L−1 was achieved without addition of buffer and methanogen inhibitor. In general, acetic, n-butyric and iso-valeric acids were the most predominant species, although different operational conditions affected the VFA concentration, profile, production rate and yield. The microbial community analysis was conducted on the reactors with the best performance, revealing that 70–90% of the microbial population was from the Clostridiales order with a strong presence from the Sporanaerobacter genus. These results demonstrated the feasibility of a VFA platform bio-refinery using high-protein wastes as substrate via mixed-culture fermentation under non-sterilised conditions.

ACS Style

Jersson Plácido; Yue Zhang. Production of volatile fatty acids from slaughterhouse blood by mixed-culture fermentation. Biomass Conversion and Biorefinery 2018, 8, 621 -634.

AMA Style

Jersson Plácido, Yue Zhang. Production of volatile fatty acids from slaughterhouse blood by mixed-culture fermentation. Biomass Conversion and Biorefinery. 2018; 8 (3):621-634.

Chicago/Turabian Style

Jersson Plácido; Yue Zhang. 2018. "Production of volatile fatty acids from slaughterhouse blood by mixed-culture fermentation." Biomass Conversion and Biorefinery 8, no. 3: 621-634.

Review
Published: 11 June 2017 in Bioengineering
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Sustainable biofuels, biomaterials, and fine chemicals production is a critical matter that research teams around the globe are focusing on nowadays. Polyhydroxyalkanoates represent one of the biomaterials of the future due to their physicochemical properties, biodegradability, and biocompatibility. Designing efficient and economic bioprocesses, combined with the respective social and environmental benefits, has brought together scientists from different backgrounds highlighting the multidisciplinary character of such a venture. In the current review, challenges and opportunities regarding polyhydroxyalkanoate production are presented and discussed, covering key steps of their overall production process by applying pure and mixed culture biotechnology, from raw bioprocess development to downstream processing.

ACS Style

Constantina Kourmentza; Jersson Plácido; Nikolaos Venetsaneas; Anna Burniol-Figols; Cristiano Varrone; Hariklia N. Gavala; Maria A. M. Reis. Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production. Bioengineering 2017, 4, 55 .

AMA Style

Constantina Kourmentza, Jersson Plácido, Nikolaos Venetsaneas, Anna Burniol-Figols, Cristiano Varrone, Hariklia N. Gavala, Maria A. M. Reis. Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production. Bioengineering. 2017; 4 (4):55.

Chicago/Turabian Style

Constantina Kourmentza; Jersson Plácido; Nikolaos Venetsaneas; Anna Burniol-Figols; Cristiano Varrone; Hariklia N. Gavala; Maria A. M. Reis. 2017. "Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production." Bioengineering 4, no. 4: 55.

Original paper
Published: 11 May 2017 in Waste and Biomass Valorization
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Two routes, esterification and membrane based solvent extraction, were tested for their efficiency to extract volatile fatty acids (VFA) (acetic, propionic, butyric, iso-butyric, iso-valeric and valeric acids) produced from the anaerobic-mixed fermentation of slaughterhouse blood. A range of operational parameters and reagents were assessed to optimize each system. Esterification recovered VFA as methyl esters with the production of ammonium sulphate as a value-added by-product of the esterification reaction. A membrane extraction system, comprised of a hydrophobic membrane and extractant using octanol/TOA (trioctylamine), was efficient to separate butyric and iso-valeric acids in favour of acetic acid from the fermentation broth. These results provided important information for the development of a carboxylate-platform bio-refinery using high-protein wastes as substrate.

ACS Style

Jersson Plácido; Yue Zhang. Evaluation of Esterification and Membrane Based Solvent Extraction as Methods for the Recovery of Short Chain Volatile Fatty Acids from Slaughterhouse Blood Anaerobic Mixed Fermentation. Waste and Biomass Valorization 2017, 9, 1767 -1777.

AMA Style

Jersson Plácido, Yue Zhang. Evaluation of Esterification and Membrane Based Solvent Extraction as Methods for the Recovery of Short Chain Volatile Fatty Acids from Slaughterhouse Blood Anaerobic Mixed Fermentation. Waste and Biomass Valorization. 2017; 9 (10):1767-1777.

Chicago/Turabian Style

Jersson Plácido; Yue Zhang. 2017. "Evaluation of Esterification and Membrane Based Solvent Extraction as Methods for the Recovery of Short Chain Volatile Fatty Acids from Slaughterhouse Blood Anaerobic Mixed Fermentation." Waste and Biomass Valorization 9, no. 10: 1767-1777.

Journal article
Published: 17 May 2016 in Bioresources and Bioprocessing
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Biodiesel, one of the most important sources of renewable energy, is produced in large quantities around the world; however, its production generates different kinds of residues and by-products which raise economic and environmental concerns. This review presents a compilation of the data on current state of transformation of residues and by-products of biodiesel industry into products that are suitable for bio-refining. The review has analyzed glycerol, biodiesel washing wastewaters, and solid residues. The technologies were described and the most significant experimental results and variables were summarized to allow researchers an easy access to this information. Keywords Biodiesel Waste glycerol Biodiesel washing wastewaters Fermentation Thermal conversion

ACS Style

Jersson Placido; Sergio Capareda. Conversion of residues and by-products from the biodiesel industry into value-added products. Bioresources and Bioprocessing 2016, 3, 733 .

AMA Style

Jersson Placido, Sergio Capareda. Conversion of residues and by-products from the biodiesel industry into value-added products. Bioresources and Bioprocessing. 2016; 3 (1):733.

Chicago/Turabian Style

Jersson Placido; Sergio Capareda. 2016. "Conversion of residues and by-products from the biodiesel industry into value-added products." Bioresources and Bioprocessing 3, no. 1: 733.

Journal article
Published: 10 February 2016 in Bioresources and Bioprocessing
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Jersson Plácido; Xiomara Chanagá; Santiago Ortiz-Monsalve; María Yepes; Amanda Mora. Degradation and detoxification of synthetic dyes and textile industry effluents by newly isolated Leptosphaerulina sp. from Colombia. Bioresources and Bioprocessing 2016, 3, 1 .

AMA Style

Jersson Plácido, Xiomara Chanagá, Santiago Ortiz-Monsalve, María Yepes, Amanda Mora. Degradation and detoxification of synthetic dyes and textile industry effluents by newly isolated Leptosphaerulina sp. from Colombia. Bioresources and Bioprocessing. 2016; 3 (1):1.

Chicago/Turabian Style

Jersson Plácido; Xiomara Chanagá; Santiago Ortiz-Monsalve; María Yepes; Amanda Mora. 2016. "Degradation and detoxification of synthetic dyes and textile industry effluents by newly isolated Leptosphaerulina sp. from Colombia." Bioresources and Bioprocessing 3, no. 1: 1.

Journal article
Published: 01 February 2016 in Sustainable Energy Technologies and Assessments
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Jersson Placido; Sergio Capareda; Raghuparty Karthikeyan. Production of humic substances from cotton stalks biochar by fungal treatment with Ceriporiopsis subvermispora. Sustainable Energy Technologies and Assessments 2016, 13, 31 -37.

AMA Style

Jersson Placido, Sergio Capareda, Raghuparty Karthikeyan. Production of humic substances from cotton stalks biochar by fungal treatment with Ceriporiopsis subvermispora. Sustainable Energy Technologies and Assessments. 2016; 13 ():31-37.

Chicago/Turabian Style

Jersson Placido; Sergio Capareda; Raghuparty Karthikeyan. 2016. "Production of humic substances from cotton stalks biochar by fungal treatment with Ceriporiopsis subvermispora." Sustainable Energy Technologies and Assessments 13, no. : 31-37.

Journal article
Published: 19 May 2015 in Bioresources and Bioprocessing
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Jersson Placido; Sergio Capareda. Ligninolytic enzymes: a biotechnological alternative for bioethanol production. Bioresources and Bioprocessing 2015, 2, 23 .

AMA Style

Jersson Placido, Sergio Capareda. Ligninolytic enzymes: a biotechnological alternative for bioethanol production. Bioresources and Bioprocessing. 2015; 2 (1):23.

Chicago/Turabian Style

Jersson Placido; Sergio Capareda. 2015. "Ligninolytic enzymes: a biotechnological alternative for bioethanol production." Bioresources and Bioprocessing 2, no. 1: 23.

Journal article
Published: 01 May 2015 in Industrial Crops and Products
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Jersson Plácido; Sergio Capareda. Production of silicon compounds and fulvic acids from cotton wastes biochar using chemical depolymerization. Industrial Crops and Products 2015, 67, 270 -280.

AMA Style

Jersson Plácido, Sergio Capareda. Production of silicon compounds and fulvic acids from cotton wastes biochar using chemical depolymerization. Industrial Crops and Products. 2015; 67 ():270-280.

Chicago/Turabian Style

Jersson Plácido; Sergio Capareda. 2015. "Production of silicon compounds and fulvic acids from cotton wastes biochar using chemical depolymerization." Industrial Crops and Products 67, no. : 270-280.