During the preparation of high dilutions, repeated external vibration (shaking) is used. We hypothesized that it was the vibration treatment, and not the negligible content of the initial substance, that underlies the activity of highly diluted preparations. In order to test this, the vibration was separated from the dilution process. After vibrating two tubes together on a vortex mixer (one containing water and the other the initial substance) the electrical conductivity and radio frequency radiation intensity of water differed from the unvibrated control, and the ability to exert a modifying effect on the target solution appeared, as assessed using ELISA and terahertz spectroscopy, appeared. Thus, the properties of the neutral carrier (water) changed after non-contact exposure to the initial substance. We have named this process ‘crossing’ and its products ‘aqueous iterations of the initial substance’. Several aqueous iterations with different physical properties were obtained, some of which have a modifying effect and others cause various chemical (catalytic) and biological (antiviral) effects similar to those of the initial substance. This indicates that during crossing, substances enter into post-vibration supramolecular interactions. At the nanoscale level, aqueous iterations and the initial substance are structurally symmetrical, which allows us to assume that the preservation of the symmetry of substances subjected to vibration treatment is the basis of the post-vibration interaction phenomenon.

Objectives. It was recently discovered that water samples with modified physicochemical properties can be obtained by successive vibration treatment of intact water together with a solution of a substance located in separate closely spaced vials. We refer to such samples as iterations. By adding the vibrational iterations into the initial substance, the physicochemical properties of the latter are changed, i.e., they demonstrate post-vibration activity. In addition, it has been shown that vibrational iterations can be obtained using water treated with a magnetic field as the initial substance. On this basis, we may hypothesize that the phenomenon of post-vibration activity is universal. To confirm this hypothesis, water treated with an electric signal having various parameters (electrochemically activated water) was used as the initial substance for the preparation of vibrational iterations.Methods. The physicochemical properties of vibrational iterations, which were obtained from electrochemically activated water, were studied by conductometry, terahertz spectroscopy, and radiometry. The effect of the initial substance or its vibrational iterations on intact water (a neutral carrier) was evaluated by dynamic light scattering. For this purpose, the intensity of light scattering by the sample and the hydrodynamic diameter of optical heterogeneities were measured. The attenuation coefficient of an additional electric signal applied to the samples was determined.Results. The obtained vibrational iterations differ from intact water and their mixtures with intact water in terms of specific electrical conductivity, power flux density of microwave radiation, as well as in the contribution of the main (Debye) relaxation process to the overall dielectric response. Mixtures of vibrational iterations with water also differ from intact water in terms of the size of optical heterogeneities. By analogy with the vibrational iterations for which solutions of high- and low-molecular-weight substances were used as the initial substance, vibrational iterations obtained using electrochemically activated water can be classified into different groups (fractions) according to their physicochemical characteristics. Different degrees of changes in the physicochemical characteristics are observed depending on the parameters of the electric signal used to obtain the initial substance. The efficiency of electrical signal propagation in these mixtures, as estimated by the signal strength attenuation coefficient, is additionally changed. The addition of the initial substance (electrochemically activated water) to intact water also leads to changes in the physicochemical properties of the resulting mixture compared to the control. Depending on the parameters of the electric signal used to obtain the initial substance, the magnitude of changes in the physicochemical characteristics of these mixtures similarly varies.Conclusions. The fundamental possibility of obtaining vibrational iterations from electrochemically activated water similarly to vibrational iterations prepared in other studies, was demonstrated. This confirms the universality of the phenomenon of post-vibration.

For many years, our research group has been studying a phenomenon initially discovered in biological models: a solution of a substance subjected to vibration treatment exerts a modifying effect on an intact sample of the same substance, altering its physicochemical and biological properties. This effect also occurs without direct contact, when the vibration-treated and intact substances are kept in separate vials. Since the emergence of this modifying effect requires minimal energy input, we suggest that it is mediated by pre-existing supramolecular mechanisms. During vibration, the molecules deviate from a specific axis of symmetry (a local stationary state), which likely activates an evolutionarily developed supramolecular control over the molecular spatial structure. While testing this hypothesis, we observed that the modifying effect can be detected at a significant distance. This finding indicates that the phenomenon cannot be explained solely by the direct electromagnetic radiation of the vibrating molecules. Instead, the vibration treatment remotely alters the electromagnetic environment of the intact substance, thereby inducing the modifying effect. While non-trivial quantum effects might underlie this process, we favor the assumption that other fundamental interactions influence the electromagnetic field.