Menopause is associated with a progressive decline in estrogen levels, which contributes to increased oxidative stress, inflammatory processes, and metabolic and bone alterations that may favor the development of chronic conditions such as osteoporosis and cardiovascular diseases. Due to the potential risks associated with long-term hormone replacement therapy, the use of nutraceutical formulations containing phytoestrogens and antioxidant compounds has emerged as a potential alternative for the management of menopausal symptoms and associated metabolic alterations. In this context, the present study aimed to conduct the pharmacotechnical and phytochemical characterization of the nutraceutical formulation Ormona^®, as well as to evaluate its antioxidant activity, cytotoxicity, anti-inflammatory potential, and molecular interactions with biological targets involved in estrogen signaling using integrated in silico and in vitro approaches. Pharmacotechnical analyses included weight variation, hardness, friability, and disintegration tests in order to evaluate the quality, uniformity, and stability of the formulation. Phytochemical screening and antioxidant activity were evaluated using the DPPH and ABTS radical-scavenging assays. In silico molecular docking studies were performed to investigate the interactions between the bioactive compounds present in the formulation and estrogen receptors and proteins involved in inflammatory pathways. In vitro assays were conducted to evaluate cytotoxicity in MRC-5 fibroblasts and anti-inflammatory activity by inhibiting nitric oxide production in macrophages. The formulation demonstrated satisfactory pharmacotechnical properties, relevant antioxidant activity, low cytotoxicity in fibroblast cells, potential anti-inflammatory activity, and favorable molecular interactions with estrogen receptors and inflammatory targets. These findings suggest that Ormona^® may represent a promising nutraceutical formulation for menopausal health, acting through antioxidant, anti-inflammatory, and estrogen receptor-related mechanisms. However, further experimental and clinical studies are necessary to confirm its biological effects and therapeutic potential.